Abstract：
AIMS:To develop a gel formulation to trigger a visual signal for rapid disclosure of the location and extent of surface contamination with viable Bacillus anthracis spores. METHODS AND RESULTS:Methylumbelliferyl-α-d-glucopyranoside was combined with hyaluronic acid to produce a gel that could be applied to a surface as a coating. It remained hydrated for a sufficient time for α-glucosidase activity present in intact B. anthracis spores to cleave the substrate and release the fluorescent product, methylumbelliferone. The presence of B. anthracis spores could be disclosed at 5 × 104 CFU per reaction test well (0·32 cm2 ) both visually and using fluorescence detection equipment. CONCLUSIONS:The disclosure gel provides a rapid, visual response to the presence of B. anthracis spores on a surface. SIGNIFICANCE AND IMPACT OF THE STUDY:The disclosure gel demonstrates the first steps towards the development of a formulation that can provide nonspecialist users with a visual alert to the presence of B. anthracis spores on a surface. It is envisioned that such a formulation would be beneficial in scenarios where exposure to spore release is a risk, and could be used in the initial assessment of equipment to aid prioritization and localized execution of a decontamination strategy.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Robinson CV,Bishop AHdoi
10.1111/jam.14226subject
Has Abstractpub_date
2019-06-01 00:00:00pages
1700-1707issue
6eissn
1364-5072issn
1365-2672journal_volume
126pub_type
杂志文章abstract：AIMS:To identify active phenolic constituents in muscadine grape skin (MGS) extracts and determine interactions among compounds while further exploring their anti-Helicobacter pylori potential in vitro. METHODS AND RESULTS:The inhibitory effects of quercetin and resveratrol, active polyphenols identified in MGS extrac...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12129
更新日期：2013-04-01 00:00:00
abstract：AIMS:To develop a method that is able to determine the microbial reduction in different dishwasher cleaning cycles and differentiate between different program parameters used. METHODS AND RESULTS:Stainless steel biomonitors were contaminated with Micrococcus luteus or Entereococcus faecium and cleaned in a specially p...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14564
更新日期：2020-05-01 00:00:00
abstract：:A total of 21 plant-associated Serratia plymuthica strains were characterized phenotypically by their nutritional patterns, susceptibility to antibiotics, antifungal and haemolytic properties, and genotypically by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA, PCR fingerprints using BOX prim...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2000.01064.x
更新日期：2000-06-01 00:00:00
abstract：AIMS:The aim of this study was to elucidate whether grape-associated fungi exert an influence on gushing by their production of surface-active compounds. METHODS AND RESULTS:In preliminary experiments, 58 grape-associated isolates of species within Penicillium and Aspergillus genera were tested for their ability to mo...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13366
更新日期：2017-03-01 00:00:00
abstract：AIM:The main aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines. METHODS AND RESULTS:Seventeen strains of V. parahaemolyticus isolated from shrimps (Penaeus monodon) and from the environments where t...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2005.02571.x
更新日期：2005-01-01 00:00:00
abstract：AIMS:The suitability of composting for disposal of livestock mortalities due to Bacillus anthracis was assessed by measuring viability of surrogate spores from two strains each of Bacillus licheniformis and Bacillus thuringiensis after a heating cycle modelled on a cattle composting study. METHODS AND RESULTS:Sporulat...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12991
更新日期：2016-01-01 00:00:00
abstract：AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12456
更新日期：2014-06-01 00:00:00
abstract：AIMS:To provide molecular fingerprinting evidence of the contribution of wildlife vectors in the on-farm epidemiology of Salmonella Enteritidis infections. METHODS AND RESULTS:Salmonella Enteritidis strains were isolated from wildlife and from farm environment samples collected in 10 egg layer farms. Isolates were typ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.01924.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:Thymol-rich medicinal plants have been used in traditional medicine to relieve infectious diseases. However, the application of essential oils as medicine is limited by its low water solubility and high vapour pressure. The objective of this study was to produce stable nanoemulsions of Thymus daenensis oil in wate...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13541
更新日期：2017-10-01 00:00:00
abstract：AIMS:To determine the effects of supplemented copper (Cu2+) on growth and viability of strains used as starters and adjunct cultures for Emmental cheese manufacture. METHODS AND RESULTS:Thirteen strains belonging to Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus rhamnosus, Streptococcus thermophilu...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03849.x
更新日期：2008-10-01 00:00:00
abstract：AIMS:To evaluate the in vitro activity of egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus. METHODS AND RESULTS:Specific IgY was produced by immunizing hens with formaldehyde-killed Staph. aureus, using a bacterial strain known to cause mastitis. The IgY, of 94% purity, was obtained from yo...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03920.x
更新日期：2008-11-01 00:00:00
abstract：AIMS:Isolate and characterize a laccase-encoding gene (lac I) of Phlebia brevispora BAFC 633, as well as cloning and expressing cDNA of lac I in Pichia pastoris. And to obtain a purified and characterized recombinant laccase to analyse the biotechnological application potential. METHODS AND RESULTS:Lac I was cloned an...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13720
更新日期：2018-06-01 00:00:00
abstract：AIMS:To characterize the bioemulsifier produced by a nonfluorescent strain of Pseudomonas putida isolated from a polluted sediment and to determine the influence of pH, temperature, media composition, and carbon and nitrogen source on growth and emulsifying activity. METHODS AND RESULTS:Different indexes were employed...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02480.x
更新日期：2005-01-01 00:00:00
abstract：:Staphylococcus aureus is an important agent of contagious bovine intramammary infections in dairy cattle. Its ability to persist inside the udder is based on the presence of important mechanisms such as its ability to form biofilms, polysaccharide capsules small colony variants, and their ability to invade professiona...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/jam.14706
更新日期：2020-11-01 00:00:00
abstract：AIMS:To study the individual and combined contribution of catechin, protocatechuic and vanillic acids to inhibit the adhesion of uropathogenic Escherichia coli (UPEC) on the surface of silicone catheters. METHODS AND RESULTS:The adhesion of UPEC to silicone catheters during the exposure to nonlethal concentrations of ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14472
更新日期：2020-02-01 00:00:00
abstract：AIMS:The influence of the cheese-making process, ripening conditions and primary starter on the viability and proteolytic activity of an adjunct culture of Lactobacillus plantarum I91 was assessed in two miniature cheese models, representative of Cremoso Argentino and Cheddar cheeses. METHODS AND RESULTS:Cheeses with ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03813.x
更新日期：2008-09-01 00:00:00
abstract：AIMS:This paper describes optimization of electrotransformation of Mu transposition complexes into Lactococcus lactis cells and identification of genes affecting nisin production. METHODS AND RESULTS:The highest transformation efficiency, 1.1 x 10(2) transformants microg(-1) of input transposon DNA, was achieved when ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03962.x
更新日期：2009-01-01 00:00:00
abstract：AIMS:The aim was to evaluate (i) the resistance of Escherichia coli BJ4 to citral in a buffer system as a function of citral concentration, treatment medium pH, storage time and initial inoculum size, (ii) the role of the sigma factor RpoS on citral resistance of E. coli, (iii) the role of the cell envelope damage in t...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04597.x
更新日期：2010-06-01 00:00:00
abstract：AIM:To assess the role of lactate as a precursor for butyrate biosynthesis in human colonic microflora. METHODS AND RESULTS:Three human faecal microfloras were incubated in vitro with media supplemented with 30 mmol l(-1) unenriched or 13C-enriched lactate. Lactate metabolism and short-chain fatty acid (SCFA) producti...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2005.02605.x
更新日期：2005-01-01 00:00:00
abstract：AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14520
更新日期：2020-03-01 00:00:00
abstract：AIMS:To validate an in vitro model for the analysis of physiological and ecological responses to sugar challenge in bacterial populations, and subsequent changes in enamel mineralization. METHODS AND RESULTS:A seven-organism bacterial consortium was grown in a biofilm mode on enamel and hydroxyapatite (HA) surfaces in...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2001.01263.x
更新日期：2001-03-01 00:00:00
abstract：AIMS:To relate sensory shelf-life of iced whole and gutted squid to bacterial growth and chemical changes. METHODS AND RESULTS:Cooked mantles from whole and gutted individuals were rejected after 10 and 12 days of storage, respectively, due to ammoniacal off-odours. Rate of production of both ammonia and trimethylamin...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2002.01604.x
更新日期：2002-01-01 00:00:00
abstract：AIMS:The current study aimed to assess the potential of a new high dose ultraviolet (UV) disinfection device to inactivate methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile and a norovirus surrogate on handheld mobile devices, and to compare the efficacy of the UV-C device to hydrogen peroxide d...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14308
更新日期：2019-08-01 00:00:00
abstract：AIMS:To develop descriptive models for the combined effect of temperature (10-40 degrees C) and water activity (0.850-0.980) on the growth of two ochratoxin A producing strains of Aspergillus carbonarius from Greek wine grapes on a synthetic grape juice medium. METHODS AND RESULTS:Fungal growth was measured as changes...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03480.x
更新日期：2007-12-01 00:00:00
abstract：:In order to characterize the subtypes of Salmonella typhi which cause sporadic disease in Taiwan, 55 isolates of Salm. typhi obtained from unrelated patients of sporadic cases during 1992-96 were subjected to chromosomal DNA digestion and pulsed field gel electrophoresis (PFGE). When DNAs of these 55 Salm. typhi strai...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1999.00720.x
更新日期：1999-05-01 00:00:00
abstract：AIM:Campylobacter species are significantly implicated in human gastrointestinal infections. Of 20 species of Campylobacter, C. jejuni, C. coli and C. lari have been considered as the most important causative agents of human infections. In order to better understand the occurrence and epidemiology of these thermophilic...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03511.x
更新日期：2007-12-01 00:00:00
abstract：AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2010.04870.x
更新日期：2011-01-01 00:00:00
abstract：AIMS:To determine the prevalence of Arcobacter in various food, animal and water sources in Turkey and to subtype the isolated strains using enterobacterial repetitive intergenic consensus (ERIC)-PCR. METHODS AND RESULTS:A total of 806 samples consisting of chicken (100) and turkey meat (100); minced beef (27); rectal...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03240.x
更新日期：2007-07-01 00:00:00
abstract：AIMS:Bacillus anthracis strains of various origins were analysed with the view to describe intrinsic and persistent structural components of the Bacillus collagen-like protein of anthracis glycoprotein associated anthrose containing tetrasaccharide in the exosporium. METHODS AND RESULTS:The tetrasaccharide consists of...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.04129.x
更新日期：2009-05-01 00:00:00
abstract：AIMS:The aims of this study were to identify the structure of antibacterial lipopeptide (LP) produced by Bacillus amyloliquefaciens strain FJAT-2349, to analyse the effects of the culture medium and temperature on LP production and to assess the biocontrol efficiency of the LPs against tomato bacterial wilt. METHODS A...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14213
更新日期：2019-05-01 00:00:00
