Abstract:
AIMS:In this work, the 'hi-tech' complex biocleaning and restoration of the 14th-century fresco Triumph of Death (5·6 × 15·0 m) at the Camposanto Monumental Cemetery (Pisa, Italy) is reported. Since 2000, the restoration based on the biological cleaning of noble medieval frescoes, has been successfully utilized in this site. METHODS AND RESULTS:The novelty of this study is the two-steps biocleaning process using Pseudomonas stutzeri A29 viable cells, previously applied for recovering other valuable frescoes. In this case, after the fresco detachment from the asbestos-cement support (eternity), both the animal glue and the residues of calcium caseinate were biologically removed respectively from the front and from the back of the fresco in 3 h as indicated by GC-MS and PY/GC-MS analyses. The data obtained during the monitoring of the biorestoration process confirmed that the adopted procedure does not leave residual cells on the fresco surfaces as showed by plate count method, ATP determination and also SEM observation. In addition, to avoid the risk of condensation phenomena after the relocation of the restored fresco sections onto the original walls, the use of a new support has been set up together with the design of a control system that allows a continuous monitoring of environmental parameters for prevention and conservation purposes. CONCLUSIONS:This large-scale biorestoration work clearly shows and confirms that this biotechnology is highly efficient, safe, noninvasive, risk-free and very competitive compared to the traditional cleaning methods, offering an unusual 'resurrection' of the degraded artworks also in very complicated and delicate conditions such as the Triumph of Death fresco, defined for its dimension and artistic importance the 'Pisa's Sistina frescoes'. SIGNIFICANCE AND IMPACT OF THE STUDY:These findings can be of significant importance for other future new restoration activities and they are crucial for determining preservation strategies in this field.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Ranalli G,Zanardini E,Andreotti A,Colombini MP,Corti C,Bosch-Roig P,De Nuntiis P,Lustrato G,Mandrioli P,Rampazzi L,Giantomassi C,Zari Ddoi
10.1111/jam.13913subject
Has Abstractpub_date
2018-09-01 00:00:00pages
800-812issue
3eissn
1364-5072issn
1365-2672journal_volume
125pub_type
杂志文章abstract:AIMS:Growth of the lactic acid bacterium (LAB) Oenococcus oeni, which is involved in malolactic fermentation during the winemaking process, is stimulated by peptides originating from yeast. In this study, we investigated the impact of peptides on O. oeni growth, peptidase activity and the expression of genes encoding t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04032.x
更新日期:2009-03-01 00:00:00
abstract:AIMS:To develop a DNA microarray for easy and fast detection of trichothecene- and moniliformin-producing Fusarium species. METHOD AND RESULTS:A DNA microarray was developed for detection and identification of 14 trichothecene- and moniliformin-producing species of the fungal genus Fusarium. The array could also diffe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03165.x
更新日期:2007-04-01 00:00:00
abstract:AIMS:Evaluation of the efficiency of peracetic acid in the disinfection of wastewater in a large treatment plant. METHODS AND RESULTS:Over a period of 18 months 30 sample collections were made, each consisting of three samples taken from: raw incoming sewage, secondary effluent (after 10-12 h) and secondary effluent d...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01451.x
更新日期:2001-11-01 00:00:00
abstract:AIMS:The aims of this study were to characterize the antibacterial activity and the chemotype of Litsea cubeba leaf essential oil (EO) harvested in North Vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (Cyprinus carpio) challenged ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13160
更新日期:2016-08-01 00:00:00
abstract::The influence of growth phase on the evolution of cellular fatty acids (CFA) and survival of Sinorhizobium and Bradyrhizobium during desiccation and storage at different levels of relative humidity (R. H.) was studied. Lactobacillic, cis vaccenic and palmitic acids were the major fatty acids of S. meliloti RCR 2011, B...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00860.x
更新日期:1999-10-01 00:00:00
abstract:AIMS:The purpose of this study was to develop a laboratory biofilm growth reactor system that simulated the toilet bowl environment and which could be used for biocide efficacy testing. METHODS AND RESULTS:A microbial biofilm reactor system incorporating intermittent flow and nutrient provision was designed. The react...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01342.x
更新日期:2001-07-01 00:00:00
abstract::A rhamnolipid-producing strain of Pseudomonas aeruginosa GL1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (PAH) as sole carbon source. Strain GL1 did not grow on PAH but grew on known degradation metabolites of phenanthrene (O-phthalic acid) and of naphthalene (salic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00412.x
更新日期:1998-05-01 00:00:00
abstract:AIMS:Coagulase-negative Staphylococcus xylosus strains are used as starter organisms for sausage fermentation. As those strains have to cope with low pH-values during fermentation, the aim of this study was to identify the acid adaptation mechanisms of S. xylosus TMW 2.1523 previously isolated from salami. METHODS AND...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14224
更新日期:2019-05-01 00:00:00
abstract:AIMS:Hypersensitivity pneumonitis of machinists associated with metalworking fluids (MWF) was recently linked to Mycobacterium immunogenum. In addition to Mycobacterium, impacts of continuous and massive contact to other micro-organisms, such as Pseudomonas, were little studied. This report intended to quantify and cha...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04433.x
更新日期:2010-02-01 00:00:00
abstract:AIMS:The aim of this study was to develop a method based on immunomagnetic capture and polymerase chain reaction (IC-PCR assay) for detection of Cryptosporidium parvum and Giardia intestinalis in sewage sludge. METHODS AND RESULTS:The detection limit of the IC-PCR assay for both organisms was 625 oocysts and cysts ml(...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01468.x
更新日期:2001-12-01 00:00:00
abstract:AIMS:The goals of this study were to determine if β-acid from hops (Humulus lupulus L.) could be used to control fructan fermentation by equine hindgut micro-organisms, and to verify the antimicrobial mode of action on Streptococcus bovis, which has been implicated in fructan fermentation, hindgut acidosis and pasture-...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12532
更新日期:2014-08-01 00:00:00
abstract:AIMS:Investigating mechanisms of lethality enhancement when Escherichia coli O157:H7, and selected E. coli mutants, were exposed to tert-butylhydroquinone (TBHQ) during ultra-high pressure (UHP) treatment. METHODS AND RESULTS:Escherichia coli O157:H7 EDL-933, and 14 E. coli K12 strains with mutations in selected genes...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03973.x
更新日期:2008-12-01 00:00:00
abstract:AIM:Survival of Escherichia coli O157:H7 and Salmonella enterica in animal waste-based composts was studied with different compost types, storage conditions and inoculum levels. METHODS AND RESULTS:A cocktail of three E. coli O157:H7 or S. enterica strains was inoculated into dairy manure-based composts (A and B) or p...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13719
更新日期:2018-05-01 00:00:00
abstract:AIMS:The isolation of high-quality RNA from cheese is a prerequisite for analysis of in situ gene expression of dairy micro-organisms. METHODS AND RESULTS:A method for rapid isolation of bacterial cells from cheese using cold citrate buffer followed by mechanical cell disruption was developed. RNA was extracted from e...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03869.x
更新日期:2008-11-01 00:00:00
abstract:AIMS:To analyse the germination and its heterogeneity of individual spores of Clostridium perfringens. METHODS AND RESULTS:Germination of individual wild-type Cl. perfringens spores was followed by monitoring Ca-dipicolinic acid (CaDPA) release and by differential interference contrast (DIC) microscopy. Following the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05135.x
更新日期:2011-11-01 00:00:00
abstract:AIMS:To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. METHODS AND RESULTS:After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated Bacillu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05395.x
更新日期:2012-10-01 00:00:00
abstract:AIMS:To investigate the microbiota in marinated, vacuum-packed pork and to characterize isolated bacteria with regard to their spoilage potential. METHODS AND RESULTS:Laboratory marinated pork meat and commercial products from three Norwegian producers were examined. Lactic acid bacteria dominated in all products at t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04183.x
更新日期:2009-06-01 00:00:00
abstract:AIMS:This study measured the survival of Listeria monocytogenes and Salmonella enterica subsp. enterica serovar Javiana over a 10-day period on four soil-free cultivation matrix (SFCM) types in the absence of microgreens and fertilizers. METHODS AND RESULTS:Coco coir (CC), a Sphagnum peat/vermiculite mix, Biostrate® a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14696
更新日期:2020-12-01 00:00:00
abstract:AIMS:To determine if nisin F-loaded self-setting brushite cement could control the growth of Staphylococcus aureus in vivo. METHODS AND RESULTS: Brushite cement was prepared by mixing equimolar concentrations of β-tricalcium phosphate and monocalcium phosphate monohydrate. Nisin F was added at 5·0, 2·5 and 1·0% (w/w)...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05241.x
更新日期:2012-04-01 00:00:00
abstract:AIMS:To identify and characterize adhesion-associated proteins in the potential probiotic Lactobacillus fermentum BCS87. METHODS AND RESULTS:Protein suspensions obtained from the treatment of Lact. fermentum BCS87 with 1 mol 1(-1) LiCl were analysed by Western blotting using HRP-labelled porcine mucus and mucin. Two a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04368.x
更新日期:2009-12-01 00:00:00
abstract::A number of bacterial strains used for meat fermentations were screened for proteolytic activity. A strain of Micrococcus which was found to be proteolytic was evaluated for the effects of environmental conditions on its proteolytic activity against pork myofibrillar proteins using response surface methodology. Three ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00829.x
更新日期:1999-12-01 00:00:00
abstract::The effect of divalent cations on the inactivation of Escherichia coli by high hydrostatic pressure was investigated. The presence of 0.5 mmol l-1 of CaCl2, MgCl2, MnCl2 and FeCl2 reduced pressure inactivation of E. coli MG1655, while 0.5 mmol l-1 of ZnCl2, NiCl2, CuCl2 and CoCl2 increased inactivation. Baroprotection...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.1998.00577.x
更新日期:1998-10-01 00:00:00
abstract:AIM:Investigation of antifungal mechanism of phenazine 1-carboxamide (PC) produced by a Pseudomonas strain MCC2142. METHODS AND RESULTS:An antifungal metabolite produced by a Pseudomonas was purified and identified as PC. Human pathogenic fungi such as Candida albicans, Candida glabrata, Cryptococcus neoformans, Fusar...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12675
更新日期:2015-01-01 00:00:00
abstract:AIMS:A survey was conducted on the ecological distribution of bacteriocin-producing lactic acid bacteria in Miso-pastes, a typical fermented food in Japan. METHODS AND RESULTS Nine Miso-pastes were sampled for isolation of bacteriocin-producers. Almost all isolated enterococcal strains produced bacteriocins but no isol...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01573.x
更新日期:2002-01-01 00:00:00
abstract::The effect of yoghurt culture Rx on the survival of Staphylococcus aureus CCM 5984 added to milk in various concentrations was observed during the fermentation and storage of yoghurt. The end of the fermentation process (3.5 h) was only accompanied by a slight reduction. During the storage of yoghurt at 4 degrees C a ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract:AIMS:To determine the major components of total and metabolically active microbial communities of yellow and grey colonizations threatening the conservation of palaeolithic paintings in Altamira Cave (Spain). METHODS AND RESULTS:Micro-organisms present in yellow and grey colonizations were determined from DNA analysis...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03594.x
更新日期:2008-03-01 00:00:00
abstract:AIMS:The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth. METHODS AND RESULTS:Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager bre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02472.x
更新日期:2005-01-01 00:00:00
abstract:AIM:To analyse the physiological response of Sphingobium sp. 22B to water stress. METHODS AND RESULTS:The strain was grown under excess of carbon source and then subjected to low (60RH) and high (18RH) water stress conditions for 96 h. Quantification of trehalose, glycogen, polyhydroxybutyrate (PHB) and transmission e...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13742
更新日期:2018-06-01 00:00:00
abstract:AIMS:This study examined the tolerance of various species of the genus Bifidobacterium to heat and oxygen and evaluated the survival of selected strains following spray drying and during storage. METHODS AND RESULTS:Nine Bifidobacterium species were considered to be relatively tolerant to both heat and oxygen and most...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02648.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To isolate and characterize copper-resistant halophilic bacteria from the polluted Maruit Lake, Egypt and identify the role of plasmids in toxic metal resistance. METHODS AND RESULTS:We isolated strain MA2, showing high copper resistance up to the 1.5 mmol l(-1) concentration; it was also resistant to other metal...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04574.x
更新日期:2010-04-01 00:00:00