Co-overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis.

Abstract:

AIMS:To improve lincomycin A production and decrease the content of byproduct lincomycin B in an industrial lincomycin-producing strain. METHODS AND RESULTS:The in silico analysis indicated that LmbW could be involved in propylproline biosynthesis of lincomyin A. In this study, we constructed an lmbW deletion mutant and found that the mutant lost the ability to produce lincomycin A, but increased the accumulation of lincomycin B. The loss of lincomycin A production can be restored by complementing the mutant with the expression of lmbW gene. When lmbW and metK (encoding S-adenosylmethionine synthetase) was co-overexpressed, lincomycin A titre was 1744·6 mg l(-1) , a 35·83% improvement over the original strain. Meanwhile, the content of lincomycin B was reduced to 4·41%, a remarkable decrease of 34·76%, compared to that of the original strain. CONCLUSIONS:lmbW encodes a C-methyltransferase involved in the biosynthesis of lincomycin A but not lincomycin B. Co-overexpression of lmbW and metK improved lincomycin A production and decreased the content of lincomycin B. SIGNIFICANCE AND IMPACT OF THE STUDY:The engineered Streptomyces lincolnensis strain shows promising application in the fermentation production of lincomycin A, which may help cut production costs and simplify downstream separation processes.

journal_name

J Appl Microbiol

authors

Pang AP,Du L,Lin CY,Qiao J,Zhao GR

doi

10.1111/jam.12919

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

1064-74

issue

4

eissn

1364-5072

issn

1365-2672

journal_volume

119

pub_type

杂志文章
  • Response to high-pressure, low-temperature treatment in vegetables: determination of survival rates of microbial populations using flow cytometry and detection of peroxidase activity using confocal microscopy.

    abstract::Application of high hydrostatic pressure (200, 300, 350 and 400 MPa) at 5 degrees C for 30 min to different micro-organisms, including Gram-positive and Gram-negative bacteria, moulds and yeasts, proved to be more effective in inactivating these organisms than treatments at 20 degrees C for 10 min and at 10 degrees C ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00701.x

    authors: Arroyo G,Sanz PD,Préstamo G

    更新日期:1999-03-01 00:00:00

  • Arbuscular mycorrhizal fungus alleviates alfalfa leaf spots caused by Phoma medicaginis revealed by RNA-seq analysis.

    abstract:AIMS:One of the major limitations to the production of alfalfa (Medicago sativa) is the fungus Phoma medicaginis, which infects alfalfa and causes leaf spots. This study aims to understand alfalfa's response to P. medicaginis infection, the colonization of arbuscular mycorrhizal fungus (AMF) and the effect of AMF on pl...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14387

    authors: Li Y,Duan T,Nan Z,Li Y

    更新日期:2019-07-16 00:00:00

  • The effect of Pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (Oreochromis niloticus).

    abstract:AIM:To assess Pediococcus acidilactici as a dietary supplement for on-growing red tilapia (Oreochromis niloticus). METHODS AND RESULTS:Tilapia were fed either a control diet or control diet supplemented with Ped. acidilactici at 10(7) CFU g(-1) for 32 days. Ped. acidilactici colonized the intestinal tract and signific...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04713.x

    authors: Ferguson RM,Merrifield DL,Harper GM,Rawling MD,Mustafa S,Picchietti S,Balcázar JL,Davies SJ

    更新日期:2010-09-01 00:00:00

  • Genotypic diversity and antifungal susceptibility pattern of Candida albicans species isolated from hospitalized pediatric patients with urinary tract infection in Iran.

    abstract:AIMS:The present study aimed to determine the microsatellite length polymorphism (MLP) genotypic patterns and antifungal susceptibility profiles of Candida albicans isolated from patients with candiduria. METHODS AND RESULTS:DNA of 50 C. albicans isolates was used for molecular identification based on the ITS1 -5.8s-I...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.15006

    authors: Gharaghani M,Rezaei-Matehkolaei A,Hardani AK,Zarei Mahmoudabadi A

    更新日期:2021-01-18 00:00:00

  • Cytotoxicity and apoptosis induction of some selected marine bacteria metabolites.

    abstract:AIMS:To study the potential apoptosis effects of cytotoxic marine bacterial metabolites on human HeLa cell line. METHODS AND RESULTS:After HeLa cells were routinely cultured, tetrazolium-based colorimetric assay for cytotoxicity was performed to screen the marine bacteria extracts showing 12 strains active. To find th...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02741.x

    authors: Lin J,Yan XJ,Zheng L,Ma HH,Chen HM

    更新日期:2005-01-01 00:00:00

  • Modelling of Reactive Black 5 decolourization in the presence of heavy metals by the newly isolated Pseudomonas aeruginosa strain Gb30.

    abstract:AIM:The effect of heavy metals presence on the decolourization of Reactive Black 5 by Pseudomonas aeruginosa was evaluated. METHODS AND RESULTS:In the current study, a newly isolated strain identified as P. aeruginosa strain Gb 30 was selected for its ability to remove high concentration of Reactive Black 5 and resist...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14262

    authors: Louati I,Hadrich B,Nasri M,Belbahri L,Woodward S,Mechichi T

    更新日期:2019-06-01 00:00:00

  • Changes in bacterial community during fermentative hydrogen and acid production from organic waste by thermophilic anaerobic microflora.

    abstract:AIMS:Changes in fermentation pattern during the treatment of organic wastes containing solid materials by thermophilic anaerobic microflora were investigated with respect to product formation and bacterial community structure during hydrogen production. METHODS AND RESULTS:Anaerobic microflora enriched from sludge com...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02939.x

    authors: Ueno Y,Sasaki D,Fukui H,Haruta S,Ishii M,Igarashi Y

    更新日期:2006-08-01 00:00:00

  • A new real-time PCR method for the identification of Salmonella Dublin.

    abstract:AIMS:Development of a real-time PCR method for the specific detection of Salmonella Dublin. METHODS AND RESULTS:The method was directed towards a Salm. Dublin-specific sequence of the vagC gene on the Salmonella virulence plasmid (pSDV) and towards Salmonella genus-specific sequence of the invA gene, serving as an int...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05378.x

    authors: Persson S,Jacobsen T,Olsen JE,Olsen KE,Hansen F

    更新日期:2012-09-01 00:00:00

  • Stenotrophomonas and Lysobacter: ubiquitous plant-associated gamma-proteobacteria of developing significance in applied microbiology.

    abstract::The exploration of new source materials and the use of alternative isolation and identification methods have led to rapid expansion in the knowledge of diversity; in Lysobacter, 11 new species having been described since 2005, and in Stenotrophomonas with six new species since 2000. The new species of Lysobacter, isol...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2672.2009.04471.x

    authors: Hayward AC,Fegan N,Fegan M,Stirling GR

    更新日期:2010-03-01 00:00:00

  • Comparison of the sensitivity of manual and automated immunomagnetic separation methods for detection of Shiga toxin-producing Escherichia coli O157:H7 in milk.

    abstract:AIM:To determine the sensitivity of methods for detection of injured and uninjured Escherichia coli O157:H7 (E. coli O157) in raw and pasteurized milk. METHODS AND RESULTS:Raw milk, pasteurized milk with 1.5% fat content and pasteurized milk with 3.5% fat content were spiked with E. coli O157 at low levels. The sample...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01646.x

    authors: Reinders RD,Barna A,Lipman LJ,Bijker PG

    更新日期:2002-01-01 00:00:00

  • Campylobacter contamination in French chicken production from farm to consumers. Use of a PCR assay for detection and identification of Campylobacter jejuni and Camp. coli.

    abstract:AIMS:Campylobacter contamination in French chicken production from the farm to the consumer was determined using a PCR assay for bacteria detection and identification. METHODS AND RESULTS:Samples were bird droppings from poultry houses, neck skins, livers, hearts, gizzards, wings, legs and escalopes from slaughterhous...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01380.x

    authors: Denis M,Refrégier-Petton J,Laisney MJ,Ermel G,Salvat G

    更新日期:2001-08-01 00:00:00

  • Highly diverse variable number tandem repeat loci in the E. coli O157:H7 and O55:H7 genomes for high-resolution molecular typing.

    abstract:AIM:Evaluation of the Escherichia coli genome for variable number tandem repeat (VNTR) loci in order to provide a subtyping tool with greater discrimination and more efficient capacity. METHODS AND RESULTS:Twenty-nine putative VNTR loci were identified from the E. coli genomic sequence. Their variability was validated...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02532.x

    authors: Keys C,Kemper S,Keim P

    更新日期:2005-01-01 00:00:00

  • A PCR-DGGE method for detection and identification of Campylobacter, Helicobacter, Arcobacter and related Epsilobacteria and its application to saliva samples from humans and domestic pets.

    abstract:AIMS:To develop a PCR-denaturing gradient gel electrophoresis (PCR-DGGE) method for the detection and identification of Campylobacter, Helicobacter and Arcobacter species (Epsilobacteria) in clinical samples and evaluate its efficacy on saliva samples from humans and domestic pets. METHODS AND RESULTS:A semi-nested PC...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03515.x

    authors: Petersen RF,Harrington CS,Kortegaard HE,On SL

    更新日期:2007-12-01 00:00:00

  • Soil microbiota respond to green manure in organic vineyards.

    abstract:AIMS:The aim of this work was to investigate the effects of biodynamic management with and without the addition of green manure, in comparison with organic management, on the microbiota in vineyards soil. METHODS AND RESULTS:High throughput sequencing was used to compare the taxonomic structure of the soil bacterial a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13606

    authors: Longa CMO,Nicola L,Antonielli L,Mescalchin E,Zanzotti R,Turco E,Pertot I

    更新日期:2017-12-01 00:00:00

  • A sensitive method for the recovery of Escherichia coli serogroup O55 including Shiga toxin-producing variants for potential use in outbreaks.

    abstract:AIM:Shiga toxin-producing Escherichia coli (STEC) cause bloody diarrhoea, kidney failure and occasionally death. However, identifying the source of infection caused by STEC other than serogroup O157 is hampered by the availability of sensitive methods for detecting these pathogens. In this study, we developed novel too...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14345

    authors: Kirchner M,Sayers E,Cawthraw S,Duggett N,Gosling R,Jenkins C,Dallman TJ,Mueller-Doblies D,Anjum MF

    更新日期:2019-09-01 00:00:00

  • Involvement of a rhamnolipid-producing strain of Pseudomonas aeruginosa in the degradation of polycyclic aromatic hydrocarbons by a bacterial community.

    abstract::A rhamnolipid-producing strain of Pseudomonas aeruginosa GL1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (PAH) as sole carbon source. Strain GL1 did not grow on PAH but grew on known degradation metabolites of phenanthrene (O-phthalic acid) and of naphthalene (salic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00412.x

    authors: Arino S,Marchal R,Vandecasteele JP

    更新日期:1998-05-01 00:00:00

  • Purification of a thermostable endochitinase from Streptomyces RC1071 isolated from a cerrado soil and its antagonism against phytopathogenic fungi.

    abstract:AIMS:The chitinolytic activity of an actinomycete, isolated from a tropical acidic ferrasol (FAO) under cerrado (savanna) vegetation, is reported. METHODS AND RESULTS:Selection of the strain was based on spot inoculation on solid colloidal chitin medium. The use of chemotaxonomic, morphological and physiological proce...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01294.x

    authors: Gomes RC,Sêmedo LT,Soares RM,Linhares LF,Ulhoa CJ,Alviano CS,Coelho RR

    更新日期:2001-04-01 00:00:00

  • Pathogenic factors in Candida biofilm-related infectious diseases.

    abstract::Candida albicans is a commonly found member of the human microflora and is a major human opportunistic fungal pathogen. A perturbation of the microbiome can lead to infectious diseases caused by various micro-organisms, including C. albicans. Moreover, the interactions between C. albicans and bacteria are considered t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13330

    authors: Hirota K,Yumoto H,Sapaar B,Matsuo T,Ichikawa T,Miyake Y

    更新日期:2017-02-01 00:00:00

  • Lactate is mainly fermented to butyrate by human intestinal microfloras but inter-individual variation is evident.

    abstract:AIM:To assess the role of lactate as a precursor for butyrate biosynthesis in human colonic microflora. METHODS AND RESULTS:Three human faecal microfloras were incubated in vitro with media supplemented with 30 mmol l(-1) unenriched or 13C-enriched lactate. Lactate metabolism and short-chain fatty acid (SCFA) producti...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02605.x

    authors: Bourriaud C,Robins RJ,Martin L,Kozlowski F,Tenailleau E,Cherbut C,Michel C

    更新日期:2005-01-01 00:00:00

  • Aerosol-phase activity of iodine captured from a triiodide resin filter on fine particles containing an infectious virus.

    abstract:AIMS:To avoid interference by water-iodine disinfection chemistry and measure directly the effect of iodine, captured from a triiodide complex bound to a filter medium, on viability of penetrating viral particles. METHODS AND RESULTS:Aerosols of MS2 coli phage were passed through control P100 or iodinated High-Efficie...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12788

    authors: Heimbuch BK,Harnish DA,Balzli C,Lumley A,Kinney K,Wander JD

    更新日期:2015-06-01 00:00:00

  • Crenarchaeota and Euryarchaeota in temperate estuarine sediments.

    abstract:AIMS:Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large vari...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01297.x

    authors: Abreu C,Jurgens G,De Marco P,Saano A,Bordalo AA

    更新日期:2001-05-01 00:00:00

  • Antimicrobial activity of trans-cinnamic acid and commonly used antibiotics against important fish pathogens and nonpathogenic isolates.

    abstract:AIMS:Antibiotics and several other chemicals have been used to prevent fish diseases. However, this situation results in economic loss for the companies in the aquaculture industry and most importantly it pollutes the environment. Cinnamic acid is a naturally occurring aromatic acid and is considered to be safe for hum...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14097

    authors: Yilmaz S,Sova M,Ergün S

    更新日期:2018-09-04 00:00:00

  • Characterization of sourdough lactic acid bacteria based on genotypic and cell-wall protein analyses.

    abstract:AIMS:To evaluate the effectiveness of two independent methods in differentiating a large population of lactic acid bacteria (LAB) isolated from wheat flours and sourdoughs and to correlate eventual differences/similarities among strains with their geographical origin and/or process parameters. METHODS AND RESULTS:One ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01874.x

    authors: Corsetti A,De Angelis M,Dellaglio F,Paparella A,Fox PF,Settanni L,Gobbetti M

    更新日期:2003-01-01 00:00:00

  • A putative new endophytic nitrogen-fixing bacterium Pantoea sp. from sugarcane.

    abstract:AIMS:To isolate and identify endophytic nitrogen-fixing bacteria in sugarcane growing in Cuba without chemical fertilizers. METHODS AND RESULTS:Two N2-fixing isolates, 9C and T2, were obtained from surface-sterilized stems and roots, respectively, of sugarcane variety ML3-18. Both isolates showed acetylene reduction a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02329.x

    authors: Loiret FG,Ortega E,Kleiner D,Ortega-Rodés P,Rodés R,Dong Z

    更新日期:2004-01-01 00:00:00

  • Characterization of chlorobenzoate degraders isolated from polychlorinated biphenyl-contaminated soil and sediment in the Czech Republic.

    abstract::Two polychlorinated biphenyl-contaminated sites in the Czech Republic, a soil at Zamberk and a sediment sludge at Milevsko, were screened for the presence of chlorobenzoate degraders. Sixteen different chlorobenzoate degraders were isolated from the soil compared with only three strains isolated from the sediment. Fro...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00830.x

    authors: Pavlû L,Vosáhlová J,Klierová H,Prouza M,Demnerová K,Brenner V

    更新日期:1999-09-01 00:00:00

  • Production of massoia lactone by Aureobasidium pullulans YTP6-14 isolated from the Gulf of Thailand and its fragrant biosurfactant properties.

    abstract:AIMS:In order to add to the existing knowledge about structural diversity of biosurfactants, marine environment was chosen to discover a new type of biosurfactant-producing fungus. METHODS AND RESULTS:A number of fungi were collected from the Gulf of Thailand and examined for biosurfactant productivities. A dimorphic ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13598

    authors: Luepongpattana S,Thaniyavarn J,Morikawa M

    更新日期:2017-12-01 00:00:00

  • Solar UV reduces Cryptosporidium parvum oocyst infectivity in environmental waters.

    abstract:AIMS:To determine the effect of solar radiation on Cryptosporidium parvum in tap and environmental waters. METHODS AND RESULTS:Outdoor tank experiments and a cell culture infectivity assay were used to measure solar inactivation of C. parvum oocysts in different waters. Experiments conducted on days with different lev...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03658.x

    authors: King BJ,Hoefel D,Daminato DP,Fanok S,Monis PT

    更新日期:2008-05-01 00:00:00

  • Novel antibacterial silver-silica surface coatings prepared by chemical vapour deposition for infection control.

    abstract:AIMS:Environmental contamination plays an important role in the transmission of infections, especially healthcare-associated infections. Disinfection transiently reduces contamination, but surfaces can rapidly become re-contaminated. Antimicrobial surfaces may partially overcome that limitation. The antimicrobial activ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12308

    authors: Varghese S,Elfakhri S,Sheel DW,Sheel P,Bolton FJ,Foster HA

    更新日期:2013-11-01 00:00:00

  • Survival of Salmonella enterica subsp. enterica serovar Javiana and Listeria monocytogenes is dependent on type of soil-free microgreen cultivation matrix.

    abstract:AIMS:This study measured the survival of Listeria monocytogenes and Salmonella enterica subsp. enterica serovar Javiana over a 10-day period on four soil-free cultivation matrix (SFCM) types in the absence of microgreens and fertilizers. METHODS AND RESULTS:Coco coir (CC), a Sphagnum peat/vermiculite mix, Biostrate® a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14696

    authors: Misra G,Gibson KE

    更新日期:2020-12-01 00:00:00

  • Application of bacteria from non-cultivated plants to promote growth, alter root architecture and alleviate salt stress of cotton.

    abstract:AIMS:Cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. This process also eliminates beneficial bacteria on the seed surface. The goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt st...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13414

    authors: Irizarry I,White JF

    更新日期:2017-04-01 00:00:00