Abstract：
:At the time of this writing, searching Google Scholar for 'light-sheet microscopy' returns almost 8500 results; over three-quarters of which were published in the last 5 years alone. Searching for other advanced imaging methods in the last 5 years yields similar results: 'super-resolution microscopy' (>16 000), 'single-molecule imaging' (almost 10 000), SPIM (Single Plane Illumination Microscopy, 5000), and 'lattice light-sheet' (1300). The explosion of new imaging methods has also produced a dizzying menagerie of acronyms, with over 100 different species of 'light-sheet' alone, from SPIM to UM (Ultra microscopy) to SiMView (Simultaneous MultiView) to iSPIM (inclined SPIM, not to be confused with iSPIM, inverted SPIM). How then is the average biologist, without an advanced degree in physics, optics, or computer science supposed to make heads or tails of which method is best suited for their needs? Let us also not forget the plight of the optical physicist, who at best might need help with obtaining healthy samples and keeping them that way, or at worst may not realize the impact their newest technique could have for biologists. This review will not attempt to solve all these problems, but instead highlight some of the most recent, successful mergers between biology and advanced imaging technologies, as well as hopefully provide some guidance for anyone interested in journeying into the world of live-cell imaging.
journal_name
Curr Opin Cell Bioljournal_title
Current opinion in cell biologyauthors
Lemon WC,McDole Kdoi
10.1016/j.ceb.2020.04.008subject
Has Abstractpub_date
2020-10-01 00:00:00pages
34-42eissn
0955-0674issn
1879-0410pii
S0955-0674(20)30054-5journal_volume
66pub_type
杂志文章,评审abstract：:Protein O-mannosylation and N-glycosylation are essential post-translational modifications, which initiate in the endoplasmic reticulum (ER). In yeast, the two glycosylation machineries act at the Sec61 translocon complex where they can even compete for certain substrate proteins. N-linked glycans play a crucial role ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2016.04.010
更新日期：2016-08-01 00:00:00
abstract：:Evolutionarily conserved from yeast to man, mitogen-activated protein kinase (MAPK) pathways respond to a variety of disparate signals which induce differentiation, proliferation, or changes in intracellular enzyme regulation. Recent advances have identified two new mammalian MAPK relatives, JNK1 and p38, and the path...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(95)80063-8
更新日期：1995-12-01 00:00:00
abstract：:Two key processes are in the basis of morphogenesis: the spatial allocation of cell types in fields of naïve cells and the regulation of growth. Both are controlled by morphogens, which activate target genes in the growing tissue in a concentration-dependent manner. Thus the morphogen model is an intrinsically quantit...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2008.01.008
更新日期：2008-04-01 00:00:00
abstract：:The activation of the serine/threonine kinase Raf-1 is proving to be an intricate multistep process. Recent advances in elucidating how Raf-1 becomes activated in response to signaling events have emphasized the role of phosphorylation and protein interactions in Raf-1 regulation. The picture clearly emerging is that ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(97)80060-9
更新日期：1997-04-01 00:00:00
abstract：:Recent evidence corroborates the idea that the structure of the nucleolus need not be strictly maintained for proper function, suggesting that the organelle is composed of supramolecular assemblies formed during rRNA synthesis. More controversial is whether the nucleolus exists in the absence of rRNA synthesis and whe...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(95)80085-9
更新日期：1995-06-01 00:00:00
abstract：:Vesicle and tubule transport containers move proteins and lipids from one membrane system to another. Newly forming transport containers frequently have electron-dense coats. Coats coordinate the accumulation of cargo and sculpt the membrane. Recent advances have shown that components of both COP1 and clathrin-adaptor...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2004.06.009
更新日期：2004-08-01 00:00:00
abstract：:The principal subunits of a large superfamily of plasma-membrane cation channels are functionally autonomous in ion conductance and in gating by membrane potential and intracellular ligands. Recent work has located the structural elements responsible for the ion conductance and gating of these channels. These studies ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(94)90083-3
更新日期：1994-08-01 00:00:00
abstract：:HOM/Hox genes are master regulatory switches that specify axial identity and control the growth and differentiation of groups of cells related by position. HOM/Hox genes function combinatorially and hierarchically to specify cell fate. Some of the genes they regulate and that mediate specific identify functions have b...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(93)90086-6
更新日期：1993-12-01 00:00:00
abstract：:Polarity is defined by the segregation of cellular components along a defined axis. To polarize robustly, cells must be able to break symmetry and subsequently amplify these nascent asymmetries. Finally, asymmetric localization of signaling molecules must be translated into functional regulation of downstream effector...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2019.05.002
更新日期：2019-10-01 00:00:00
abstract：:Classical heterochromatin chromosomal landmarks, such as centromeres and telomeres, are characterized by specific chromatin signatures. Among these, the incorporation of histone variants has recently emerged as an important feature. Using the centromere as a paradigm, we consider the role of histone variant dynamics i...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2011.03.006
更新日期：2011-06-01 00:00:00
abstract：:The epithelial apical-junctional complex is a key regulator of cellular functions. In addition, it is an important target for microbial pathogens that manipulate the cell to survive, proliferate and sometimes persist within a host. Out of a myriad of potential molecular targets, some bacterial and viral pathogens have...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2003.12.002
更新日期：2004-02-01 00:00:00
abstract：:Force arising from actin polymerization and myosin activity drives a number of different actin-based cell movements. Several new reports support previous data suggesting that actin polymerization drives lamellipodial protrusion and bacterial propulsion, and one report describes a more indirect role for actin assembly ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(94)90120-1
更新日期：1994-02-01 00:00:00
abstract：:Cyclic AMP regulates a myriad of cellular processes through the cAMP-dependent protein kinase A. In many cases, activation of protein kinase A leads to altered patterns of gene transcription. Identification of the cAMP-response-element-binding-protein as a major nuclear substrate for protein kinase A accounts for many...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(91)90113-d
更新日期：1991-12-01 00:00:00
abstract：:Cell size control and homeostasis is a long-standing subject in biology. Recent experimental work provides extensive evidence for a simple, quantitative size homeostasis principle coined adder (as opposed to sizer or timer). The adder principle provides unexpected insights into how bacteria maintain their size without...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2016.02.004
更新日期：2016-02-01 00:00:00
abstract：:The assembly of intermediate filament (IF) arrays involves the recruitment of a complex set of cell-type-specific IF-associated proteins. Some of them are integral membrane proteins, others act as crosslinking proteins with vectorial binding activities, and yet others comprise motor proteins. In vivo IFs appear to be ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(99)00060-5
更新日期：2000-02-01 00:00:00
abstract：:Recently there have been advances in studies of the molecular biology of the receptor for CNTF. In contrast with the receptors for other known neurotrophic factors, which belong to the family of receptor tyrosine kinases, the CNTF receptor belongs to the family of cytokine receptors. This review will describe the stru...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(93)90117-9
更新日期：1993-04-01 00:00:00
abstract：:Mammalian Unc-51-like kinases 1 and 2 (ULK1 and ULK2) belong to the ULK/Atg1 family of serine/threonine kinases, which are conserved from yeast to mammals. Although ULK/Atg1 is best known for regulating flux through the autophagy pathway, it has evolutionarily conserved noncanonical functions in protein trafficking th...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2017.02.011
更新日期：2017-04-01 00:00:00
abstract：:The multimolecular WAVE regulatory (WRC) and Arp2/3 complexes are primarily responsible to generate pushing forces at migratory leading edges by promoting branch elongation of actin filaments. The architectural complexity of these units betrays the necessity to impose a tight control on their activity. This is exerted...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2013.04.001
更新日期：2013-10-01 00:00:00
abstract：:Analysis of complex and redundant pathways that control proliferation, differentiation, apoptosis and DNA damage response by global genome wide analysis is an intensive area of investigation aimed at identifying unique molecular signatures of prognostic significance in cancer. An alternative approach is to focus on th...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2007.10.005
更新日期：2007-12-01 00:00:00
abstract：:The actin supergene family encodes a number of structurally related, but perhaps functionally distinct, protein isoforms that regulate contractile potential in muscle tissues and help to control the shape as well as the motility of non-muscle cells. In spite of the documented conservation amongst isoactin genes and th...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(05)80007-9
更新日期：1993-02-01 00:00:00
abstract：:Cellular asymmetries have been proposed to play a role in plant embryogenesis. Genetic studies of Arabidopsis and other experimental approaches in several plant species have addressed the origins of cellular asymmetry in specific cases. Although zygote polarity, which precedes the formation of the apical-basal axis of...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(97)80087-7
更新日期：1997-12-01 00:00:00
abstract：:One of the most notable trends in human evolution is the dramatic increase in brain size that has occurred in the great ape clade, culminating in humans. Of particular interest is the vast expanse of the cerebral cortex, which is believed to have resulted in our ability to perform higher cognitive functions. Recent in...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2005.11.004
更新日期：2006-02-01 00:00:00
abstract：:Members of the kinesin superfamily of microtubule-based motors and the myosin superfamily of actin-based motors that move 'backwards' have been identified. As the core catalytic domains of myosins and kinesins are similar in structure, this raises the intriguing questions of how direction reversal is accomplished and ...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(00)00170-8
更新日期：2001-02-01 00:00:00
abstract：:Recent advances in the study of protein translocation across the membrane of the endoplasmic reticulum include insights into the mechanism of signal-sequence function. Biochemical and genetic studies have provided further evidence that lumenal proteins perform direct roles in secretory protein translocation and in the...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(02)00353-8
更新日期：2002-08-01 00:00:00
abstract：:Studies of yeast transcription factors have contributed greatly to understanding basic molecular mechanisms of eukaryotic gene regulation, largely due to powerful genetic approaches that are unavailable in other organisms. The broad outlines of these mechanisms are fairly well understood, and there is an increasing nu...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(93)90018-l
更新日期：1993-06-01 00:00:00
abstract：:Mitochondria are classically known as the essential energy producers in cells. As such, the activation of mitochondrial metabolism upon cellular differentiation was deemed a necessity to fuel the high metabolic needs of differentiated cells. However, recent studies have revealed a direct role for mitochondrial activit...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2017.11.003
更新日期：2017-12-01 00:00:00
abstract：:Modulation of energy metabolism is emerging as a key aspect associated with cell fate transition. The establishment of a correct metabolic program is particularly relevant for neural cells given their high bioenergetic requirements. Accordingly, diseases of the nervous system commonly involve mitochondrial impairment....
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2017.12.004
更新日期：2017-12-01 00:00:00
abstract：:Recently, findings regarding a group of cancer predisposition and chromosome instability syndromes, Nijmegen breakage syndrome (NBS), the ataxia-telangiectasia-like disorder (A-TLD) and ataxia telangiectasia have shed light on the unexpected role of recombinational DNA repair proteins in DNA-damage-dependent cell-cycl...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0955-0674(00)00091-0
更新日期：2000-06-01 00:00:00
abstract：:The Drosophila decapentaplegic gene, the Xenopus activin genes and the genes encoding the mouse bone morphogenetic proteins are transforming growth factor-beta-related genes whose roles in development are the focus of current studies. They exhibit elaborate patterns of expression during development, and the protein pr...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/0955-0674(91)90112-c
更新日期：1991-12-01 00:00:00
abstract：:Throughout their lifetimes, all cells experience force. These forces are sensed by cell surface adhesion receptors, such as the cadherins and integrins. Much attention has focused on identifying how these adhesion receptors transmit force. In contrast, less is known regarding how these force-activated pathways are int...
journal_title：Current opinion in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.ceb.2018.05.004
更新日期：2018-10-01 00:00:00
