Abstract：
BACKGROUND AND OBJECTIVE:Enamel matrix proteins are involved in the development and regeneration of root cementum and in its attachment to dentin; however, the mechanisms through which this occurs have yet to be elucidated. The present study was therefore carried out to evaluate the mitogenic and proliferative responses of human periodontal fibroblast (HPLF) cells to Emdogain (EMD), and the potential role of cyclooxygenase 2 (COX-2) in this process. MATERIAL AND METHODS:We investigated the effects of EMD on 5-bromo-2'-deoxyuridine (BrdU) incorporation, colchicine freezing of mitosis, XTT [2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction and Trypan Blue dye exclusion, with or without celecoxibe, a selective cyclooxygenase-2 (COX-2) inhibitor; we also evaluated the expression of COX-2 mRNA and COX-2 protein in response to EMD. RESULTS:EMD significantly enhanced mitosis in, and proliferation of, human periodontal ligament fibroblasts in a dose-dependent manner; however, there was a small increase of DNA synthesis only in response to a high dose of EMD (200 μg/mL). EMD (100 and 200 μg/mL) elicited an increase in COX-2 expression (p ≤ 0.05). Celecoxibe (20 μm) diminished the EMD-induced mitosis and proliferation of HPLF cells (p ≤ 0.05). CONCLUSION:Celecoxibe hampered EMD-induced mitosis and proliferation, which, in association with EMD-increased COX-2 expression, indicates that COX-2 may be involved in the proliferative response of HPLF cells to EMD.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Khedmat S,Seyedabadi M,Ghahremani MH,Ostad SNdoi
10.1111/j.1600-0765.2010.01313.xsubject
Has Abstractpub_date
2011-02-01 00:00:00pages
67-73issue
1eissn
0022-3484issn
1600-0765journal_volume
46pub_type
杂志文章abstract：:The 3 human salivary cystatins S, SA and SN are multifunctional proteins that possess a cysteine protease inhibitory property, but their ability to act as such is very different (SN > SA > S). One form, S, also appears to possess antibacterial properties towards the bacterium Porphyromonas gingivalis, often associated...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1999.tb02279.x
更新日期：1999-11-01 00:00:00
abstract：:The three-dimensional physiological tooth movement synchronized with heartbeat, periodontal pulsation, has already been demonstrated by previous researchers. The purpose of this study was to estimate physiological tooth movements within clinically normal periodontal tissues, thus establishing periodontal pulsation sta...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2001.00665.x
更新日期：2002-04-01 00:00:00
abstract：:This study reports the immunohistochemical localization of TGF-beta receptor type II (T beta R-II) and type III (T beta R-III) in cells of the forming periodontal ligament (PDL) in rat first molar roots. Mandibular periodontium was obtained from 3, 6 and 12-wk-old rats. This represented tissue from the initial, pre-ma...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1999.tb02230.x
更新日期：1999-02-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Current epidemiological works have suggested that chronic infections, such as periodontitis, are associated with an increased risk of cardiovascular diseases, including hypertrophy and heart failure. However, mechanisms behind the association are not known. The aim of this study was to evaluate...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12380
更新日期：2017-04-01 00:00:00
abstract：:Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. In the present study we analysed both the cystatin activity and the different cystatin isoforms in gingival crevicular fluid and saliva samples of nine periodontitis patients. All crevicular fluid s...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1997.tb00935.x
更新日期：1997-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Retinoic acid (RA) exerts a wide variety of effects on development, cellular differentiation and homeostasis in various tissues. However, little is known about the effects of RA on the differentiation of periodontal ligament cells. In this study, we investigated whether RA can affect the dexam...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2005.00811.x
更新日期：2005-12-01 00:00:00
abstract：:Bursts of periodontal destruction are modelled as percolation-driven phase shift events. Percolation theory, though widely used to study the flow of liquids through porous nonliving materials (e.g., soils and rocks), can be applied to bacterial or toxicological penetration of living tissue in only an approximate sense...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:The effect of enamel matrix derivative (EMD) on bone differentiation remains unclear. Transforming growth factor beta1 (TGF-beta1) is reported to be contained in EMD. The aim of this study was to clarify the effect of EMD on osteoblastic cell differentiation and the possible role of TGF-beta1. ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2007.01010.x
更新日期：2008-04-01 00:00:00
abstract：:Using the high iron diamine thiocarbohydrazide silver proteinate (HID-TCH-SP) staining technique, we investigated ultrastructural localization of sulfated glycosaminoglycans (GAGs) in the rat gingiva shortly after eruption, especially those associated with internal and external basal laminae. In the apical portion of ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1989.tb02006.x
更新日期：1989-05-01 00:00:00
abstract：:Polymorphonuclear neutrophils (PMNs) are essential in host defense against periodontopathic bacteria such as Porphyromonas gingivalis. The uptake of immunoglobulin G (IgG)-opsonized bacteria via IgG Fc receptors (Fc gamma R) on PMN constitutes a central defense mechanism in periodontium. Fc gamma RIIIb is the most abu...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2000.035002065.x
更新日期：2000-04-01 00:00:00
abstract：:Thirteen periodontally healthy subjects were monitored clinically for 6-12 months. Clinical measurements at 6-weekly intervals included duplicate PD measurements, presence of plaque, redness, and bleeding on probing. Baseline measurements consisted of 2 visits 1 wk apart. Microbial samples were taken from 11 of the su...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1996.tb00484.x
更新日期：1996-04-01 00:00:00
abstract：:Recently, nitric oxide (NO) has been shown to be vital in inflammatory processes. Nitric oxide synthase (NOS) exists in three different isoforms, two constitutively produced with physiological roles, and an inducible form, iNOS, which is involved in inflammation. This study examined the localisation of iNOS in biopsie...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2000.035006369.x
更新日期：2000-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Methylglyoxal is a toxic product derived from glucose metabolism that plays a role in inflammation, diabetes and aging. In addition, the periodontal pathogen Tannerella forsythensis may also generate this compound. However, the effects of methylglyoxal on gingival cells are still poorly unders...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12365
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Recent data have suggested that in the past 15 years there has been a dramatic increase in the incidence of diabetes mellitus in the USA. However, evidence suggests that approximately one-third of diabetes cases remain undiagnosed. Because 60% of Americans see a dentist at least once per year f...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2007.00983.x
更新日期：2007-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Neutrophil extracellular traps (NETs) are a recently discovered antimicrobial mechanism used by neutrophils that have been proposed as an intervention in the pathogenesis of periodontitis. The objective of our study was to characterize the expression of NETs in gingival tissues with periodontit...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12621
更新日期：2019-06-01 00:00:00
abstract：:Accumulating evidence indicates that TNFalpha plays an important role in the pathogenesis of periodontitis, but the effect of TNFalpha on the degradation of the periodontal ligament is not well understood. This study used reverse transcriptase-PCR to investigate the effects of TNFalpha on matrix metalloproteinase (MMP...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2002.00656.x
更新日期：2002-06-01 00:00:00
abstract：OBJECTIVES AND BACKGROUND:Endothelin-1 (ET-1) is a 21-amino acid peptide with multifunctional regulation. ET-1 expresses in various cells during inflammation. The present study aimed to examine the ET-1 expression in oral epithelial cells after infection with the periodontal pathogen and to investigate the presence of ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2003.00668.x
更新日期：2003-08-01 00:00:00
abstract：:The diagnosis of periodontal disease is commonly based on objective evaluations of the patient's medical/dental history as well as clinical and radiographic examinations. However, periodontal disease should also be evaluated subjectively through measures that quantify its impact on oral health-related quality of life....
journal_title：Journal of periodontal research
pub_type： 杂志文章,评审
doi：10.1111/jre.12436
更新日期：2017-08-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:The jaw bone, unlike most other bones, is derived from neural crest stem cells, so we hypothesized that it may have different characteristics to bones from other parts of the body, especially in the nature of its periosteum. The periosteum exhibits osteogenic potential and has received consider...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12229
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVES:Previous studies have suggested an association between chronic infection and cardiovascular disease, and combined infection/inflammation is frequently related with hypertriglyceridemia, which may be a risk factor of cardiovascular disease. In fact, experimental transient or intermittent administration of lip...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2004.00716.x
更新日期：2004-04-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Salvia miltiorrhiza Bunge (Labiatae), or Danshen, is a Chinese medicine used for treatment of cardiac diseases by improving blood circulation and inhibiting inflammatory responses. In this study, we aimed to determine whether an ethanol extract of S. miltiorrhiza can ameliorate tissue damage ca...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12292
更新日期：2016-02-01 00:00:00
abstract：:The influence of occlusal loading on periodontal fibroblasts was investigated in hypoloaded (shortened out of occlusion), functionally loaded and hyperloaded (constant linguointrusive mechanical loads of 9.4 +/- 0.06 g) lower left rat incisors. One hour following injection of 3H-thymidine, half of the animals in each ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1994.tb01087.x
更新日期：1994-01-01 00:00:00
abstract：:This study uses cytokeratins (CK) as markers to investigate the phenotype of the junctional epithelium (JE) in partially erupted human teeth. The gingival samples, which were clinically healthy, were carefully dissected from the teeth. Cryostat sections were cut for histological staining, immunofluorescence microscopy...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1994.tb01212.x
更新日期：1994-05-01 00:00:00
abstract：:The role of bacteria in the initiation of periodontitis is well-documented and the end result, destruction of the alveolar bone and periodontal connective tissue, is readily observed; but the events occurring between these two points in time remain obscure and are the focus of this paper. Bacteria induce tissue destru...
journal_title：Journal of periodontal research
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0765.1991.tb01649.x
更新日期：1991-05-01 00:00:00
abstract：:Quantitative assessment of osseous changes attributable to periodontal disease is made possible by digital subtraction radiography. Tissues through which x rays travel to produce dental radiographs essential to this process alter the energy spectrum of the beam such that calibration errors result when densitometry is ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1989.tb00857.x
更新日期：1989-01-01 00:00:00
abstract：:Growth factors are known to play a major role in the regeneration of the periodontium. Basic fibroblast growth factor (bFGF) is a polypeptide growth factor considered to have a role in chemotaxis and mitogenesis of periodontal ligament (PDL) cells. The aim of this study was to assess the effect of bFGF on the transcri...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2001.360201.x
更新日期：2001-04-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Factors such as vascularization of the periodontium, inflammatory reactions and immune response affect the oral environment and ecology, decreasing host resistance and promoting the development of symptoms and the advancement of periodontal disease. Fatigue also influences the hypothalamic-pitu...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12189
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVES:Chronic periodontitis is a bone destructive inflammatory disease with an adverse impact on general health and suggested underlying factors in common with osteoporosis. A few studies have examined the possible relationship between chronic periodontitis and osteoporosis; however, the results remain inconclusiv...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12464
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to evaluate the effects of non-surgical periodontal therapy on gingival crevicular fluid levels of matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and prostaglandin E2 (PGE2 ) in patients with rheumatoid arthritis (RA) with periodontal disease. MATERIAL AND METHODS:Twenty-s...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12337
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVE:The responsiveness of the sympathetic nervous system (SNS) and the hypothalamic--pituitary--adrenal (HPA) axis plays a major role in immune regulation and for the outcome of infections and inflammatory disorders. This study was designed to investigate whether chemical SNS denervation with the noradrenaline-se...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2005.00803.x
更新日期：2005-08-01 00:00:00
