Effect of loading on the migration of periodontal fibroblasts in the rat incisor.

Abstract:

:The influence of occlusal loading on periodontal fibroblasts was investigated in hypoloaded (shortened out of occlusion), functionally loaded and hyperloaded (constant linguointrusive mechanical loads of 9.4 +/- 0.06 g) lower left rat incisors. One hour following injection of 3H-thymidine, half of the animals in each group were killed, while the remaining rats were killed 2 weeks later. The decalcified incisors were embedded in glycolmethacrylate and sectioned (2 microns) serially, perpendicularly to the long tooth axis. Labeled and unlabeled fibroblasts in the tooth-related periodontal ligament were counted in 8 x 80 microns consecutive layers. Cell density (CD) and labeling index (LI) were plotted according to their location on the apico-incisal and cementum-bone axes. Loading caused a decrease in CD and a shift of cells from the cementum towards the middle of the ligament, proportionally to load intensity and duration. The average tooth-to-bone movement of the cells was 2 microns/day in the hypoloaded and 4 microns/day in the two loaded groups. The mean daily tooth eruption rate was 975 +/- 60 microns, 499 +/- 18 microns and 103 +/- 27 microns in the hypo-, functionally- and hyperloaded teeth, respectively. The respective concomitant average daily cell migration rates in the incisal direction were 786 microns, 500 microns, and 500 microns, i.e. 80%, 100% and 485% of the tooth eruption rates. The gross disparity between cell velocity and tooth movement under conditions of restrained eruption indicates active motility of the fibroblasts, rather than their passive tooth-eruption dependent translation.

journal_name

J Periodontal Res

authors

Michaeli Y,Shamir D,Weinreb M,Steigman S

doi

10.1111/j.1600-0765.1994.tb01087.x

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

25-34

issue

1

eissn

0022-3484

issn

1600-0765

journal_volume

29

pub_type

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