Abstract:
BACKGROUND AND OBJECTIVE:The transplantation of cell sheets of mesenchymal stem cells (MSCs) is expected to be the next generation of periodontal regenerative therapy. An adequate method of multilayering MSCs has yet to be established. When cell sheets proliferate, they usually contract and detach from culture dishes and then the proliferation of cells in the contracted areas is arrested. ROCK-mediated contractile force causes cell contraction. Although multilayer formation medium (MFM) stimulated the proliferation of growth-arrested confluent MSCs, MSCs detached from the culture dish. Therefore, we investigated the effects of ROCK inhibitor Y-27632 on the proliferation and detachment of confluent MSCs, and examined the ability of cells to differentiate within the cell sheets. MATERIAL AND METHODS:Confluent MSCs were cultured in MFM containing transforming growth factor-β1, ascorbic acid and fetal bovine serum either with or without Y-27632. Cell proliferation was examined by bromodeoxyuridine incorporation assays and total DNA measurement. Sheet contractions were examined by light microscopy and stereomicroscopy. Multilayer formations and focal adhesion assembly were observed with confocal microscopy. Characteristic of cells were examined by flow cytometric analysis. Osteoblast lineage differentiation was observed with alkaline phosphatase and alizarin red S staining. Adipocyte lineage differentiation was observed with oil red O staining. RESULTS:The addition of Y-27632 to MFM prevented the cell sheets from detaching and did not inhibit MSC growth. The cell numbers cultured with MFM/Y-27632 were significantly higher than that obtained with MFM-only on day 4. Cell sheets detached from the culture dish on day 4, and the number of bromodeoxyuridine-positive cells in the detached area decreased. Cells in the cell sheets had similar characteristics to primary MSCs, and differentiated into osteoblast and adipocyte lineages. CONCLUSION:Y-27632 both prevented the MSC sheets from detaching and maintained the multilayered proliferation of confluent MSCs by MFM, and then cells in the sheets had differentiation potency.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Nakamura K,Yoshimura A,Kaneko T,Sato K,Hara Ydoi
10.1111/jre.12114subject
Has Abstractpub_date
2014-06-01 00:00:00pages
363-70issue
3eissn
0022-3484issn
1600-0765journal_volume
49pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVE:Biofilm formation occurs through the events of cooperative growth and competitive survival among multiple species. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans are important periodontal pathogens. The aim of this study was to demonstrate competitive or cooperative interact...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12006
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Studies have shown that bacterial plaque and the associated gingival inflammation increase the severity of gingival overgrowth induced by cyclosporine-A (CsA). This in vitro study aimed to evaluate the effect of CsA on the activities of MMPs from the co-culture of human gingival fibroblasts and...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01450.x
更新日期:2012-08-01 00:00:00
abstract::The present study was carried out to examine the ultrastructural localization of laminin and type-IV collagen in the junctional epithelium of rat molar gingiva by means of the indirect immunoperoxidase method. Intense laminin reaction occurred in both the internal and the external basal laminae. Laminin reaction produ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1990.tb00930.x
更新日期:1990-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Enamel matrix proteins are involved in the development and regeneration of root cementum and in its attachment to dentin; however, the mechanisms through which this occurs have yet to be elucidated. The present study was therefore carried out to evaluate the mitogenic and proliferative response...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2010.01313.x
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Dental rubber dams (RDs) were used as barrier membranes in guided tissue regeneration for the treatment of periodontal intraosseous defects with acceptable clinical results. The aim of the present study was to investigate the effects of autoclave sterilization on properties of RD as related to ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2003.00693.x
更新日期:2003-10-01 00:00:00
abstract::The adherence of Actinomyces naeslundii to human buccal mucosa is mediated by specific interactions between the bacterial cell surface fimbriae and complementary beta-linked galactoside receptors on the epithelial cell surface. The buccal mucosa and the bacteria that colonize its surface are constantly bathed in saliv...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1991.tb01632.x
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Several studies have hypothesized that periodontal disease may increase the risk of pre-eclampsia. The correlation between the two diseases would probably be based on hypertension-related cytokine release in the local periodontal environment. The aim of this study was to evaluate the associatio...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01368.x
更新日期:2011-08-01 00:00:00
abstract::An epidemiological association between periodontitis and cardiovascular disease has been reported in multiple studies. Various mechanisms have been proposed as potential explanations for this association, including a common factor that predisposes certain individuals to a hyper-responsive inflammatory response. Variat...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1999.tb02265.x
更新日期:1999-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:During cyclosporine-induced gingival overgrowth, the homeostatic balance of gingival connective tissue is disrupted leading to fibrosis. Galectins are glycan-binding proteins that can modulate a variety of cellular processes including fibrosis in several organs. Here, we study the role of galec...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12761
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Current epidemiological works have suggested that chronic infections, such as periodontitis, are associated with an increased risk of cardiovascular diseases, including hypertrophy and heart failure. However, mechanisms behind the association are not known. The aim of this study was to evaluate...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12380
更新日期:2017-04-01 00:00:00
abstract::This study compared a rapid, colorimetric DNA probe assay designed to be performed in a dental office within 40 min, with anaerobic culture and indirect immunofluorescence microscopy (IFM) for detection of Bacteroides forsythus and Porphyromonas gingivalis in subgingival plaque samples. The DNA probe assay used the Pe...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1998.tb02299.x
更新日期:1998-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Cigarette smoking has detrimental effects on periodontal tissue, and is known to be a risk factor for periodontal disease, including the loss of alveolar bone and ligament tissue. However, the direct effects of cigarette smoking on periodontal tissue remain unclear. Recently, we demonstrated th...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12355
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:One of the major pathologic patterns in periodontitis represents an imbalance among the production of free radicals and local antioxidants resulting in periodontal tissue destruction. The objective of the study was to investigate the influence of non-surgical periodontal treatment on salivary a...
journal_title:Journal of periodontal research
pub_type: 杂志文章,随机对照试验
doi:10.1111/jre.12088
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The aim of this study was to investigate reactions of periodontal ligament epithelial cell clusters and major histocompatibility complex class II (OX6)-immunopositive cells to simultaneously induced tooth movement and periodontitis employing Waldo's method. MATERIAL AND METHODS:Elastic gums we...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01376.x
更新日期:2011-10-01 00:00:00
abstract::A single-blind investigation was designed to study the effects of piroxicam in preventing gingival inflammation and plaque formation in beagle dogs. Twelve 1-year-old beagles were brought to optimum oral hygiene and gingival health. Thereafter, they were fed a moist plaque-promoting diet and were divided into three gr...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1991.tb01643.x
更新日期:1991-05-01 00:00:00
abstract:OBJECTIVE:To study the expression and role in vigabatrin (VGB)-induced gingival enlargement of Ki-67 antigen and p27KIP1, p21WAF1, and p53, proteins that activate or inhibit cell-cycle progression. MATERIALS AND METHODS:Six patients treated with VGB for partial epileptic seizures refractory to classic anticonvulsant t...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2004.00711.x
更新日期:2004-02-01 00:00:00
abstract::It is well recognized that human periodontal ligament cells (PDL cells) may represent local immune cells of the periodontal tissues. However, it is unclear whether they represent "true" immune cells, since they can produce pro-inflammatory cytokines not only after stimulation with bacterial lipopolysaccharides but als...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12823
更新日期:2020-12-10 00:00:00
abstract::Interleukin-1 (IL-1) molecules, IL-1 alpha and IL-1 beta are cytokines involved in the acute-phase response against infection and in the pathogenesis of periodontal destruction. Administration of exogenous IL-1 receptor antagonist (IL-1ra) is effective in reducing the inflammatory reactions mediated by IL-1. However, ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb00568.x
更新日期:1997-08-01 00:00:00
abstract::Quantitative assessment of osseous changes attributable to periodontal disease is made possible by digital subtraction radiography. Tissues through which x rays travel to produce dental radiographs essential to this process alter the energy spectrum of the beam such that calibration errors result when densitometry is ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1989.tb00857.x
更新日期:1989-01-01 00:00:00
abstract::Fibroblast function in gingival tissue is thought to be regulated by the local cellular environment--both the extracellular matrix and soluble factors. In an attempt to artificially re-create this situation fibroblasts have been cultured within 3-dimensional collagen gels in an environment more physiologically compara...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1994.tb01227.x
更新日期:1994-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Neutrophils are the primary white blood cells that are recruited to fight the initial phases of microbial infections. While healthy norms have been determined for circulating blood neutrophil counts in order to identify patients with suspected systemic infections, the levels of oral neutrophils...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12211
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND:The role of water in the etiology of periodontal disease is poorly understood. OBJECTIVES:The objective of this study was to examine the association amongst water softness, risk for periodontitis, and smoking status. METHODS:We examined the association between use of water 'softening and conditioning syste...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2004.00769.x
更新日期:2004-12-01 00:00:00
abstract::The goal of a single step therapy has been an important consideration in the development of guided tissue regeneration devices. It would spare the patient from the need for repeated surgery and eliminate many problems associated with a non-resorbable barrier. Animal studies of a collagen membrane extracted from porcin...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1998.tb02196.x
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Nicotine, the main psychoactive component of tobacco, affects cell metabolism, proliferation, adhesion and, importantly, the osteogenic differentiation of fibroblasts. Approximately 15% of all orthodontic patients are adults among who one-fifth are smokers. Hence, it is necessary to have insig...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12545
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Obesity has been implicated as a risk factor for several chronic health conditions. Recent studies have reported a relationship between obesity and periodontitis, but few studies have investigated this relationship in adolescents. The purpose of the present study was to investigate the relation...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2007.01063.x
更新日期:2008-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:It has been suggested that aggressive periodontitis has a genetic basis. Monocyte chemoattractant protein 1 (MCP-1) plays a critical role in the recruitment of monocytes and the development of periodontitis. The -2518MCP-1(A/G) polymorphism has been implicated as a risk or susceptibility factor...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01426.x
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:Mast cells are a prominent cell type in the gingival infiltrate in periodontitis. In this study we examined the expression by gingival mast cells of matrix metalloproteinases, MMP-1, MMP-2, MMP-8 and the tissue inhibitors of metalloproteinases, TIMP-1 and TIMP-2. METHODS:Gingival specimens from 12 human imm...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2003.00687.x
更新日期:2003-12-01 00:00:00
abstract::Prevotella intermedia has recently been re-defined and a new species, Prevotella nigrescens has been proposed. However, there is little data available on the incidence of these new species in periodontal health or disease. Black-pigmented anaerobes isolated from diseased and healthy subgingival sites were identified b...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1995.tb02129.x
更新日期:1995-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Gingival recession is defined as soft and hard tissue displacement resulting in root surface exposure. The optimal outcome of gingival recession treatment is complete, predictable and long-lasting root coverage with a significant level of tissue regeneration. Tissue engineering, which applies a...
journal_title:Journal of periodontal research
pub_type: 杂志文章,随机对照试验
doi:10.1111/jre.12215
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The purpose of this in vitro investigation was to study the effects of human cytomegalovirus infection on the mRNA expression for collagens I and III and for matrix metalloproteinases 1 and 2 in gingival fibroblasts. MATERIAL AND METHODS:Gingival fibroblasts were experimentally infected with t...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2007.01053.x
更新日期:2008-12-01 00:00:00