Abstract：
BACKGROUND AND OBJECTIVE:During cyclosporine-induced gingival overgrowth, the homeostatic balance of gingival connective tissue is disrupted leading to fibrosis. Galectins are glycan-binding proteins that can modulate a variety of cellular processes including fibrosis in several organs. Here, we study the role of galectin-8 (Gal-8) in the response of gingival connective tissue cells to cyclosporine. METHODS:We used human gingival fibroblasts and mouse NIH3T3 cells treated with recombinant Gal-8 and/or cyclosporine for analyzing specific mRNA and protein levels through immunoblot, real-time polymerase chain reaction, ELISA and immunofluorescence, pull-down with Gal-8-Sepharose for Gal-8-to-cell surface glycoprotein interactions, short hairpin RNA for Gal-8 silencing and Student's t test and ANOVA for statistical analysis. RESULTS:Galectin-8 stimulated type I collagen and fibronectin protein levels and potentiated CTGF protein levels in TGF-β1-stimulated human gingival fibroblasts. Gal-8 interacted with α5β1-integrin and type II TGF-β receptor. Gal-8 stimulated fibronectin protein and mRNA levels, and this response was dependent on FAK activity but not Smad2/3 signaling. Cyclosporine and tumor necrosis factor alpha (TNF-α) increased Gal-8 protein levels. Finally, silencing of galectin-8 in NIH3T3 cells abolished cyclosporine-induced fibronectin protein levels. CONCLUSION:Taken together, these results reveal for the first time Gal-8 as a fibrogenic stimulus exerted through β1-integrin/FAK pathways in human gingival fibroblasts, which can be triggered by cyclosporine. Further studies should explore the involvement of Gal-8 in human gingival tissues and its role in drug-induced gingival overgrowth.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Smith PC,Metz C,de la Peña A,Oyanadel C,Avila P,Arancibia R,Vicuña L,Retamal C,Barake F,González A,Soza Adoi
10.1111/jre.12761subject
Has Abstractpub_date
2020-10-01 00:00:00pages
724-733issue
5eissn
0022-3484issn
1600-0765journal_volume
55pub_type
杂志文章abstract：BACKGROUND AND OBJECTIVE:Porphyromonas gingivalis has been shown to actively invade endothelial cells and induce vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) overexpression. Nucleotide-binding oligomerization domain 1 (NOD1) is an intracellular pattern recognition reporter, ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12192
更新日期：2015-04-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:The aim of this study was to evaluate the effect of radiotherapy on the osseointegration of dental implants in the tibia and the action of platelet-rich plasma (PRP) as an adjuvant therapy. MATERIAL AND METHODS:A total of 18 rabbits received two implants in each tibial epiphysis, totalizing 7...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12416
更新日期：2017-06-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Long noncoding RNAs (lncRNAs) play critical and complex roles in regulating various biological processes of periodontitis. This bioinformatic study aims to construct a putative competing endogenous RNA (ceRNA) network by integrating lncRNA, miRNA and mRNA expression, based on high-throughput R...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12539
更新日期：2018-08-01 00:00:00
abstract：BACKGROUND:Porphyromonas gingivalis, a black-pigmented, gram-negative anaerobe, is found in periodontitis lesions and its presence in subgingival plaque significantly increases the risk for periodontitis. We have previously shown that patients with aggressive forms of periodontitis that are seropositive for P. gingival...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2004.00735.x
更新日期：2004-08-01 00:00:00
abstract：:The aim of this study was to conduct a systematic review to compare the clinical outcomes of two different resorbable collagen membranes in terms of regenerated bone volume, postoperative complications and membrane degradation during bone regeneration procedures. Randomized controlled trials (RCT) or controlled trials...
journal_title：Journal of periodontal research
pub_type： 杂志文章,评审
doi：10.1111/jre.12470
更新日期：2017-12-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to evaluate the presence of factor XIIIa+ dendritic cells and S-100 protein+ Langerhans' cells in the gingival epithelium and connective tissue of periodontal pockets, before and after non-surgical periodontal therapy. BACKGROUND:The microbial flora in periodontal pockets provok...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2004.00764.x
更新日期：2004-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:As epithelial cells function as a mechanical barrier, the permeability of the gingival epithelial cell layer indicates a defensive capability against invasion by periodontal pathogens. We have reported the expression of claudin-1 and E-cadherin, key regulators of permeability, in the gingival j...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2011.01404.x
更新日期：2012-02-01 00:00:00
abstract：:The matrix metalloproteinases (MMPs) collagenase, gelatinase A (72 kDa gelatinase), stromelysin, and their specific inhibitor TIMP-1 (tissue inhibitor of metalloproteinases), were immunolocalized using specific polyclonal antisera in gingival tissues from 21 patients with chronic inflammatory periodontal disease. Mono...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1994.tb01100.x
更新日期：1994-03-01 00:00:00
abstract：:Vascularization of the periodontal ligament was examined in developing upper first molars of rats from 5 to 30 d after birth with light and scanning electron microscopy. Formation of the vascular network in the periodontal ligament (PDL) started with the beginning of root formation. The PDL vessels derived from the ba...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2000.035004201.x
更新日期：2000-08-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Urokinase-plasminogen activator (uPA) is a serine protease expressed at high basal level in normal gingival cervical fluid. Despite its known pathologic role in tissue proteolysis in periodontitis, little is known concerning uPA physiological function in oral tissue. Recent evidence in cancer c...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12576
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Retinoic acid (RA) exerts a wide variety of effects on development, cellular differentiation and homeostasis in various tissues. However, little is known about the effects of RA on the differentiation of periodontal ligament cells. In this study, we investigated whether RA can affect the dexam...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2005.00811.x
更新日期：2005-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Although induction of heme oxygenase-1 by H2O2 has been reported, the protective role of heme oxygenase-1 against the cytotoxic and osteoclastogenic effects of H2O2 have not been elucidated in human periodontal ligament cells. The aim of this work was to investigate the defense mechanism of hem...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2006.00953.x
更新日期：2007-08-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Cigarette smoking has detrimental effects on periodontal tissue, and is known to be a risk factor for periodontal disease, including the loss of alveolar bone and ligament tissue. However, the direct effects of cigarette smoking on periodontal tissue remain unclear. Recently, we demonstrated th...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12355
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Lipopolysaccharide (LPS) on the cell wall of periodontal pathogens is a major mediator of the inflammatory response and can enhance alveolar bone resorption in periodontitis. Bone sialoprotein is an early marker of osteoblast differentiation. The proinflammatory cytokine, interleukin-8 (IL-8), ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12193
更新日期：2015-04-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration, and it has been reported that EMD can induce the formation of osteoclasts in mouse marrow cultures. In the present study, we investigated the mechanisms of EMD-induced osteoclast formation using a mous...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2005.00868.x
更新日期：2006-08-01 00:00:00
abstract：:We investigated the co-localization of lysosomal cathepsins B, H and L, and horseradish peroxidase (HRP) in junctional epithelial (JE) cells both as a fluid-phase endocytotic marker to demonstrate the fluid-phase endocytotic capacity of JE cells, and to understand the morphological relationships of the endocytosed for...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1997.tb00575.x
更新日期：1997-11-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Cigarette smoking is a major risk factor for periodontitis, and smoking perturbs neutrophil reactive oxygen species production. This study tested the hypothesis that cigarette smoke extract (CSE) and its components/metabolites nicotine, cotinine and thiocyanate (SCN-), may influence neutrophil ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12542
更新日期：2018-08-01 00:00:00
abstract：:The present study was carried out to examine the ultrastructural localization of laminin and type-IV collagen in the junctional epithelium of rat molar gingiva by means of the indirect immunoperoxidase method. Intense laminin reaction occurred in both the internal and the external basal laminae. Laminin reaction produ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1990.tb00930.x
更新日期：1990-11-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Porphyromonas gingivalis lipopolysaccharide (LPS) is a ligand for cell surface toll-like receptors (TLR), TLR2 and TLR4 while stimulation of either leads to cardioprotection. We hypothesized that: (1) pretreatment with P. gingivalis LPS at appropriate concentrations would induce cardioprotectio...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2009.01206.x
更新日期：2010-02-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Periodontitis may alter the systemic condition in patients with diabetes and hence interfere with glycemic control. The objective of this study was to determine the quantifiable changes in glycated hemoglobin (HbA1C) after periodontal non-surgical therapy plus azithromycin in a mixed population...
journal_title：Journal of periodontal research
pub_type： 杂志文章,随机对照试验
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更新日期：2013-12-01 00:00:00
abstract：OBJECTIVES:Chronic periodontitis is a bone destructive inflammatory disease with an adverse impact on general health and suggested underlying factors in common with osteoporosis. A few studies have examined the possible relationship between chronic periodontitis and osteoporosis; however, the results remain inconclusiv...
journal_title：Journal of periodontal research
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doi：10.1111/jre.12464
更新日期：2017-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:It remains controversial whether or not the junctional epithelium cells that are directly attached to teeth migrate on the enamel surface, as those cells are able to adhere firmly to the enamel. The aim of this study was to investigate the expression patterns of laminin gamma(2), integrin beta(...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2009.01231.x
更新日期：2010-04-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:The effect of enamel matrix derivative (EMD) on bone differentiation remains unclear. Transforming growth factor beta1 (TGF-beta1) is reported to be contained in EMD. The aim of this study was to clarify the effect of EMD on osteoblastic cell differentiation and the possible role of TGF-beta1. ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2007.01010.x
更新日期：2008-04-01 00:00:00
abstract：:Multinucleated cells (MNCs) that appeared after hydroxyapatite (HAP) implantation into experimentally-produced bone defects in rat periodontal tissues were investigated both ultrastructurally and ultracytochemically. At day 5 after implantation, MNCs first appeared along the HAP surface. They had no features of typica...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1992.tb02085.x
更新日期：1992-01-01 00:00:00
abstract：:The effectiveness of an ideal antimicrobial agent depends on its ability to kill microbes with minimal toxicity to host cells. Depending on the treatment regimen, antimicrobial agents come into contact with host cells for various intervals of time. Sanguinarium (SANG), chlorhexidine (CHX) and tetracycline (TET) are 3 ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1997.tb00542.x
更新日期：1997-04-01 00:00:00
abstract：:Fibroblast function in gingival tissue is thought to be regulated by the local cellular environment--both the extracellular matrix and soluble factors. In an attempt to artificially re-create this situation fibroblasts have been cultured within 3-dimensional collagen gels in an environment more physiologically compara...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1994.tb01227.x
更新日期：1994-09-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Adipocyte fatty acid-binding protein (A-FABP) is expressed in adipocytes, macrophages and microvascular endothelial cells, and it plays a central role in inflammation, atherosclerosis and metabolic responses. This pilot study investigated the effect of nonsurgical periodontal therapy on the ser...
journal_title：Journal of periodontal research
pub_type： 临床试验,杂志文章
doi：10.1111/jre.12009
更新日期：2013-06-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Long-term tooth retention is the ultimate goal of periodontal therapy. Aim of this study was to evaluate tooth loss (TL) during 10 years of supportive periodontal therapy (SPT) in periodontal compromised patients and to identify factors influencing TL on patient level. MATERIAL AND METHODS:Pat...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12786
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:After tooth formation is complete, it is suggested that continuity exists between the epithelial cell rests of Malassez (ERM), reduced enamel epithelium (REE) and subsequently the junctional epithelium. However, the junctional epithelium was reported to differ from REE and ERM. The developmenta...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12272
更新日期：2015-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Few studies estimated the joint effect of implant design (length and diameter), peri-implant, and occlusal variables on early marginal bone loss. The monitoring of these factors during the first year after implant loading may be effective in preventing early implant failure. This prospective lo...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12699
更新日期：2020-04-01 00:00:00
