Abstract:
BACKGROUND AND OBJECTIVE:Although induction of heme oxygenase-1 by H2O2 has been reported, the protective role of heme oxygenase-1 against the cytotoxic and osteoclastogenic effects of H2O2 have not been elucidated in human periodontal ligament cells. The aim of this work was to investigate the defense mechanism of heme oxygenase-1 on H2O2-induced cytotoxicity and to analyze the expression of receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin as markers for osteoclast differentiation in periodontal ligament cells. MATERIAL AND METHODS:Using human periodontal ligament cells, cytotoxicity was measured by the 3,4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) assay, and expression of heme oxygenase-1, RANKL, and osteoprotegerin mRNA was determined by reverse transcription-polymerase chain reaction. RESULTS:H2O2 produced a cytotoxic effect by reducing the cell viability and enhancing the expression of heme oxygenase-1 and RANKL mRNAs in a concentration- and time-dependent manner. Additional experiments revealed that heme oxygenase-1 inducer (hemin), a membrane-permeable cGMP analog (8-bromo-cGMP), carbon monoxide, extracellular signal-regulated kinase, p38 mitogen-activated protein kinase inhibitor, protein kinase inhibitor (KT5823), and nuclear factor-kappaB inhibitor (pyrrolidine dithiocarbamate) also blocked the effects of H2O2 on cell viability and RANKL mRNA expression in periodontal ligament cells. CONCLUSION:These data suggest that heme oxygenase-1 induction plays a protective role in periodontal ligament cells against the cytotoxic and RANKL-inducing effects of H2O2, through multiple signaling pathways.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Pi SH,Kim SC,Kim HT,Lee HJ,Lee SK,Kim ECdoi
10.1111/j.1600-0765.2006.00953.xsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
331-9issue
4eissn
0022-3484issn
1600-0765pii
JRE953journal_volume
42pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVE:The jaw bone, unlike most other bones, is derived from neural crest stem cells, so we hypothesized that it may have different characteristics to bones from other parts of the body, especially in the nature of its periosteum. The periosteum exhibits osteogenic potential and has received consider...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12229
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The field of salivary diagnostics lacks an accepted and validated biomarker of alveolar bone remodeling. To address this, we examined levels of salivary biomolecules specifically associated with biological aspects of bone remodeling in subjects with chronic periodontitis in a case-control study...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01445.x
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Dental rubber dams (RDs) were used as barrier membranes in guided tissue regeneration for the treatment of periodontal intraosseous defects with acceptable clinical results. The aim of the present study was to investigate the effects of autoclave sterilization on properties of RD as related to ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2003.00693.x
更新日期:2003-10-01 00:00:00
abstract:OBJECTIVE:To study the expression and role in vigabatrin (VGB)-induced gingival enlargement of Ki-67 antigen and p27KIP1, p21WAF1, and p53, proteins that activate or inhibit cell-cycle progression. MATERIALS AND METHODS:Six patients treated with VGB for partial epileptic seizures refractory to classic anticonvulsant t...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2004.00711.x
更新日期:2004-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Ciclosporin A (CsA)-induced gingival overgrowth is attributed to an exaggerated accumulation of extracellular matrix, which is mainly due to an increased expression of transforming growth factor-β1 (TGF-β1). Herein, the in vitro investigation of effects of overexpression of Smad7, a TGF-β1 sign...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01412.x
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Interleukin-6 (IL-6) is a key proinflammatory cytokine that has been considered to be important in the pathogenesis of periodontal disease. Therefore, host-modulatory agents directed at inhibiting IL-6 appear to be beneficial in terms of attenuating periodontal disease progression and potential...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12054
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND/OBJECTIVES:PTH plays an important role in bone remodeling, and different actions have been reported depending on its administration method. iPSCs are promising as a cell source for regeneration of periodontal tissue due to their ability of proliferation and pluripotency. However, the effects of PTH on iPSCs ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12762
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Periodontitis may alter the systemic condition in patients with diabetes and hence interfere with glycemic control. The objective of this study was to determine the quantifiable changes in glycated hemoglobin (HbA1C) after periodontal non-surgical therapy plus azithromycin in a mixed population...
journal_title:Journal of periodontal research
pub_type: 杂志文章,随机对照试验
doi:10.1111/jre.12058
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Diabetes mellitus is believed to increase the risk and severity of periodontitis. However, less evidence is available on the converse effects of periodontitis on diabetes. The objective of the study was to investigate to what degree experimental periodontitis induced by Porphyromonas gingivalis...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12041
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Osteonecrosis of the jaw (ONJ) is associated with bisphosphonate (BP) therapy. BPs alter osteoblast production of mediators of osteoclastogenesis, including interleukin (IL)-6, RANKL and osteoprotegerin (OPG), a RANKL antagonist. This can inhibit bone turnover and lead to necrosis. There is lit...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2010.01306.x
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The association between periodontal disease and nutrient intake was examined using linked data from the 2005 National Health and Nutrition Survey, the Comprehensive Survey of Living Conditions and the Survey of Dental Diseases from the same year 'using linked data from the National Health and N...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12375
更新日期:2017-02-01 00:00:00
abstract::Growth factors are known to play a major role in the regeneration of the periodontium. Basic fibroblast growth factor (bFGF) is a polypeptide growth factor considered to have a role in chemotaxis and mitogenesis of periodontal ligament (PDL) cells. The aim of this study was to assess the effect of bFGF on the transcri...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2001.360201.x
更新日期:2001-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Periodontal ligament stem cells (PDLSCs) have potential for osteogenic differentiation and show a great foreground in treating bone diseases. Histone three lysine 27 (H3K27) demethylase lysine demethylase 6A (KDM6A) is a critical epigenetic modifier and plays an important role in regulating ost...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12830
更新日期:2020-12-24 00:00:00
abstract::Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. In the present study we analysed both the cystatin activity and the different cystatin isoforms in gingival crevicular fluid and saliva samples of nine periodontitis patients. All crevicular fluid s...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb00935.x
更新日期:1997-10-01 00:00:00
abstract:BACKGROUND:The role of water in the etiology of periodontal disease is poorly understood. OBJECTIVES:The objective of this study was to examine the association amongst water softness, risk for periodontitis, and smoking status. METHODS:We examined the association between use of water 'softening and conditioning syste...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2004.00769.x
更新日期:2004-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Statins are inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase and have anti-inflammatory effects independent of cholesterol lowering. Recent clinical studies have indicated that statin intake has a beneficial effect on periodontal disease. However, the underlying mechanisms have not...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12132
更新日期:2014-08-01 00:00:00
abstract::Regeneration of cementum, ligament, bone and new attachment was achieved by introducing mixtures of recombinant human platelet-derived growth factor and insulin-like growth factor into debrided lesions of experimentally induced periodontitis in monkeys. This growth factor therapeutic regimen induced the regeneration o...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1992.tb01679.x
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Cyclosporine A treatment is important in the therapy of a number of medical conditions; however, alveolar bone loss is an important negative side-effect of this drug. As such, we evaluated whether concomitant administration of simvastatin would minimize cyclosporine A-associated alveolar bone l...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2008.01143.x
更新日期:2009-08-01 00:00:00
abstract::This study investigates a potential role for TGF beta 1 in the pathogenesis of cyclosporin A-induced gingival overgrowth (CsA-OG). TGF beta 1 was localized immunohistochemically in the connective tissue of both normal gingiva and CsA-OG. Intense staining for TGF beta 1 was detected at the tips of the dermal papillae o...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1998.tb02290.x
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Salvia miltiorrhiza Bunge (Labiatae), or Danshen, is a Chinese medicine used for treatment of cardiac diseases by improving blood circulation and inhibiting inflammatory responses. In this study, we aimed to determine whether an ethanol extract of S. miltiorrhiza can ameliorate tissue damage ca...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12292
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Adipocyte fatty acid-binding protein (A-FABP) is expressed in adipocytes, macrophages and microvascular endothelial cells, and it plays a central role in inflammation, atherosclerosis and metabolic responses. This pilot study investigated the effect of nonsurgical periodontal therapy on the ser...
journal_title:Journal of periodontal research
pub_type: 临床试验,杂志文章
doi:10.1111/jre.12009
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND:A tumor-necrosis factor-alpha (TNF-alpha) antagonist, the WP9QY peptide, was designed based on the crystal structure of the TNF-beta/TNF-receptor complex in order to overcome the disadvantages of macromolecules such as antibodies or soluble receptors by reducing the molecular size of TNF-alpha antagonists. I...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2005.00812.x
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:As epithelial cells function as a mechanical barrier, the permeability of the gingival epithelial cell layer indicates a defensive capability against invasion by periodontal pathogens. We have reported the expression of claudin-1 and E-cadherin, key regulators of permeability, in the gingival j...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01404.x
更新日期:2012-02-01 00:00:00
abstract::Interleukin-8 (IL-8) is believed to play an important role in the pathogenesis of various forms of periodontitis. In addition, the anti-IL-8 autoantibody has been recently recognized as a potent modulator of IL-8 function. In the current study, the concentrations of IL-8 and its autoantibody in gingival crevicular flu...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2003.02001.x
更新日期:2003-02-01 00:00:00
abstract::Karyometric and cytophotometric measurements of DNA and RNA have been evaluated in gingival graft epithelium. The study was conducted during the first 20 hours from the time of grafting when the graft epithelium undergoes a process of autolysis. Both basal cell and prickle cell layers were used. The results showed an ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1975.tb00002.x
更新日期:1975-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Neutrophil extracellular traps (NETs) are a recently discovered antimicrobial mechanism used by neutrophils that have been proposed as an intervention in the pathogenesis of periodontitis. The objective of our study was to characterize the expression of NETs in gingival tissues with periodontit...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12621
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effects of non-surgical periodontal therapy on gingival crevicular fluid levels of matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and prostaglandin E2 (PGE2 ) in patients with rheumatoid arthritis (RA) with periodontal disease. MATERIAL AND METHODS:Twenty-s...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12337
更新日期:2016-10-01 00:00:00
abstract::Colonization or emergence of microbial pathogens may result in tissue destruction by activation of one or more of five distinct host degradative pathways (matrix metalloproteinase pathway, plasminogen-dependent pathway, phagocytic pathway, PMN-serine proteinase pathway and osteoclastic bone resorption) or by direct cl...
journal_title:Journal of periodontal research
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0765.1993.tb02113.x
更新日期:1993-11-01 00:00:00
abstract::The formation of new cementum is an important issue in clinical periodontology, as cementum is required to provide attachment for newly formed periodontal tissues to the root surface. In this study a model of cementogenesis in vitro was used in order to test the effects of root surface demineralization on the migratio...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb00546.x
更新日期:1997-05-01 00:00:00
abstract::Black-pigmented bacteria which produce cytotoxic metabolic end-products and cell membrane-associated proteases have been reported to play an important role in the pathogenesis of periodontal diseases. These bacterial virulence factors can be modified by the environmental conditions including nutrients supplied various...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2001.360602.x
更新日期:2001-12-01 00:00:00