Abstract:
BACKGROUND AND OBJECTIVE:Osteonecrosis of the jaw (ONJ) is associated with bisphosphonate (BP) therapy. BPs alter osteoblast production of mediators of osteoclastogenesis, including interleukin (IL)-6, RANKL and osteoprotegerin (OPG), a RANKL antagonist. This can inhibit bone turnover and lead to necrosis. There is little information on the contribution of gingival fibroblasts, near bone-resorption sites, to the IL-6/RANKL/OPG network, the effects of BPs, or fibroblast involvement in ONJ pathogenesis. Therefore, the objective of this study was to determine the effects of alendronate and pamidronate on the constitutive production, or the lipopolysaccharide (LPS)- or IL-1β-stimulated production, of IL-6, RANKL and OPG by human gingival fibroblasts. MATERIAL AND METHODS:Human gingival fibroblasts were derived from explants obtained from healthy individuals with noninflamed gingiva. Cytotoxicity was determined by measuring the activity of a mitochondrial enzyme. Fibroblasts were pre-incubated or not with BPs (0.01 nm-1 μm), then incubated or not with LPS or IL-1β. The concentrations of IL-6, OPG and RANKL were measured using ELISA. Data were analyzed using analysis of variance (ANOVA) and Scheffé's F procedure. RESULTS:LPS and BPs were not cytotoxic. The cells produced IL-6, OPG and RANKL, all of which were stimulated by IL-1β or LPS (p ≤ 0.04). BPs generally increased the production of IL-6 and OPG (p ≤ 0.04) and decreased the production of RANKL (p ≤ 0.02). BPs generally further increased the production of LPS- or IL-1β-stimulated IL-6 (p ≤ 0.04) and had no effect on, or further increased, the production of LPS- or IL-1β-stimulated OPG (p ≤ 0.04). BPs decreased the production of LPS- or IL-1β-stimulated RANKL (p ≤ 0.04) and decreased constitutive, LPS-stimulated and IL-1β-stimulated RANKL/OPG ratios (p ≤ 0.02). CONCLUSION:The action of alendronate and pamidronate on human gingival fibroblasts, through altering the production of RANKL and OPG, appears to contribute to a microenvironment favoring the inhibition of bone resorption and ONJ.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Tipton DA,Seshul BA,Dabbous MKhdoi
10.1111/j.1600-0765.2010.01306.xsubject
Has Abstractpub_date
2011-02-01 00:00:00pages
39-47issue
1eissn
0022-3484issn
1600-0765pii
JRE1306journal_volume
46pub_type
杂志文章abstract::Effects of lathyrogens such as aminoacetonitrile (AAN), beta-aminopropionitrile (BAPN) and cysteamine--known inhibitors of cross-linking of collagen--on the mechanical strength of the periodontal ligament of the rat mandibular first molar were examined by measuring the ultimate load required to extract the tooth from ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1989.tb00880.x
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The field of salivary diagnostics lacks an accepted and validated biomarker of alveolar bone remodeling. To address this, we examined levels of salivary biomolecules specifically associated with biological aspects of bone remodeling in subjects with chronic periodontitis in a case-control study...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01445.x
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Diabetes mellitus is believed to increase the risk and severity of periodontitis. However, less evidence is available on the converse effects of periodontitis on diabetes. The objective of the study was to investigate to what degree experimental periodontitis induced by Porphyromonas gingivalis...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12041
更新日期:2013-10-01 00:00:00
abstract::The aim of this study was to compare the percentage of vital microorganisms (= microbial vitality) of saliva with that of supragingival plaque both collected at various times during the early phases of de novo plaque formation. Between intervals of optimal oral hygiene, 14 healthy participants refrained from all oral ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb00529.x
更新日期:1997-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The jaw bone, unlike most other bones, is derived from neural crest stem cells, so we hypothesized that it may have different characteristics to bones from other parts of the body, especially in the nature of its periosteum. The periosteum exhibits osteogenic potential and has received consider...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12229
更新日期:2015-08-01 00:00:00
abstract:BACKGROUND/OBJECTIVES:PTH plays an important role in bone remodeling, and different actions have been reported depending on its administration method. iPSCs are promising as a cell source for regeneration of periodontal tissue due to their ability of proliferation and pluripotency. However, the effects of PTH on iPSCs ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12762
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Factors such as vascularization of the periodontium, inflammatory reactions and immune response affect the oral environment and ecology, decreasing host resistance and promoting the development of symptoms and the advancement of periodontal disease. Fatigue also influences the hypothalamic-pitu...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12189
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Recent data have suggested that in the past 15 years there has been a dramatic increase in the incidence of diabetes mellitus in the USA. However, evidence suggests that approximately one-third of diabetes cases remain undiagnosed. Because 60% of Americans see a dentist at least once per year f...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2007.00983.x
更新日期:2007-12-01 00:00:00
abstract::Deepened periodontal pockets exert a significant pathological burden on the host and its immune system, particularly in a patient with generalized moderate to severe periodontitis. This burden is extensive and longitudinal, occurring over decades of disease development. Considerable diagnostic and prognostic successes...
journal_title:Journal of periodontal research
pub_type: 杂志文章,评审
doi:10.1111/jre.12585
更新日期:2019-02-01 00:00:00
abstract::Prevotella intermedia has recently been re-defined and a new species, Prevotella nigrescens has been proposed. However, there is little data available on the incidence of these new species in periodontal health or disease. Black-pigmented anaerobes isolated from diseased and healthy subgingival sites were identified b...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1995.tb02129.x
更新日期:1995-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:During cyclosporine-induced gingival overgrowth, the homeostatic balance of gingival connective tissue is disrupted leading to fibrosis. Galectins are glycan-binding proteins that can modulate a variety of cellular processes including fibrosis in several organs. Here, we study the role of galec...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12761
更新日期:2020-10-01 00:00:00
abstract::Recent reports suggest that specific serum IgG subclasses are a feature of several forms of periodontitis. GCF antibodies are both serum-derived and locally produced by the abundant plasma cells of the diseased periodontal tissue. Previous work has shown that crevicular fluid (GCF) levels of IgG may be reduced in acti...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb01401.x
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Forsythia detaching factor (FDF) is a putative virulence factor of Tannerella forsythia that induces detachment of adherent cells and interleukin-8 production in human fibroblasts. The objective of the present study was to clarify the relationship between anti-FDF IgG levels in gingival crevicu...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2010.01283.x
更新日期:2010-10-01 00:00:00
abstract::The exact cell type and site(s) involved in interleukin-1 (IL-1) production during gingival inflammation was determined by combining immunohistochemistry and in situ hybridization. IL-1 messenger RNA (mRNA)-expressing cells in human inflamed gingiva were identified as macrophages. The rate of IL-alpha mRNA expression ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1993.tb01048.x
更新日期:1993-01-01 00:00:00
abstract::The cytolethal distending toxin (Cdt) of Actinobacillus actinomycetemcomitans, a periodontal pathogen, is a newly described cytotoxin with immunosuppressive properties, capable of causing cell cycle arrest of lymphocytes. The objectives of this study were to investigate the occurrence of A. actinomycetemcomitans with ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2002.01618.x
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Probiotics create a biofilm and protect the oral tissues against the action of periodontal pathogenic bacteria. The aim of this study was to evaluate the effects of the oral probiotic Lactobacillus reuteri Prodentis upon the peri-implant health of edentulous patients with dental implants and p...
journal_title:Journal of periodontal research
pub_type: 杂志文章,随机对照试验
doi:10.1111/jre.12264
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The prevalence of chronic renal disease in industrialized countries is increasing, and chronic renal disease and periodontitis can have significant, reciprocal effects. The aim of this study was to evaluate the associations between specific clinical parameters and the levels of tumor necrosis f...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2009.01252.x
更新日期:2010-08-01 00:00:00
abstract::It has been assumed that there is a relationship between periodontal diseases and diabetes mellitus, however the putative periodontal microorganisms in non-diabetes mellitus (non-DM) individuals and non-insulin-dependent diabetes mellitus (NIDDM) patients have not been well studied. In this study, the detection rates ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2001.90613.x
更新日期:2001-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The gingiva is the first oral tissue directly exposed to cigarette smoke (CS). Exposure to CS compromises the structure and function of gingival tissue. Damaging or altering the gingival epithelium leads to a compromised protective barrier of the periodontium, resulting in several diseases. The...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12343
更新日期:2016-10-01 00:00:00
abstract::Growth factors are known to play a major role in the regeneration of the periodontium. Basic fibroblast growth factor (bFGF) is a polypeptide growth factor considered to have a role in chemotaxis and mitogenesis of periodontal ligament (PDL) cells. The aim of this study was to assess the effect of bFGF on the transcri...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2001.360201.x
更新日期:2001-04-01 00:00:00
abstract::The adherence of Actinomyces naeslundii to human buccal mucosa is mediated by specific interactions between the bacterial cell surface fimbriae and complementary beta-linked galactoside receptors on the epithelial cell surface. The buccal mucosa and the bacteria that colonize its surface are constantly bathed in saliv...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1991.tb01632.x
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Long noncoding RNAs (lncRNAs) play critical and complex roles in regulating various biological processes of periodontitis. This bioinformatic study aims to construct a putative competing endogenous RNA (ceRNA) network by integrating lncRNA, miRNA and mRNA expression, based on high-throughput R...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12539
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:Alveolar bone resorption is a characteristic feature of periodontal diseases and involves the removal of both the mineral and organic constituents of the bone matrix, which is caused by either multinucleated osteoclast cells or matrix metalloproteinases (MMPs). The gram-negative bacterium, Porphyromonas ging...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2004.00743.x
更新日期:2004-10-01 00:00:00
abstract:OBJECTIVES:Chronic periodontitis is a bone destructive inflammatory disease with an adverse impact on general health and suggested underlying factors in common with osteoporosis. A few studies have examined the possible relationship between chronic periodontitis and osteoporosis; however, the results remain inconclusiv...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12464
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Occlusal trauma is an important factor that influences the progression of periodontitis, but it is unclear whether occlusal trauma influences periodontal destruction at the onset of periodontitis. We established an experimental periodontitis model with both site-specific loss of attachment and ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12109
更新日期:2014-06-01 00:00:00
abstract::The matrix metalloproteinases (MMPs) collagenase, gelatinase A (72 kDa gelatinase), stromelysin, and their specific inhibitor TIMP-1 (tissue inhibitor of metalloproteinases), were immunolocalized using specific polyclonal antisera in gingival tissues from 21 patients with chronic inflammatory periodontal disease. Mono...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1994.tb01100.x
更新日期:1994-03-01 00:00:00
abstract::Recently, nitric oxide (NO) has been shown to be vital in inflammatory processes. Nitric oxide synthase (NOS) exists in three different isoforms, two constitutively produced with physiological roles, and an inducible form, iNOS, which is involved in inflammation. This study examined the localisation of iNOS in biopsie...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2000.035006369.x
更新日期:2000-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The expression of A Disintegrin and Metalloproteinase 8 (ADAM8) is associated with several inflammatory diseases. Elevated ADAM8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM8 expression in chroni...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12426
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration, and it has been reported that EMD can induce the formation of osteoclasts in mouse marrow cultures. In the present study, we investigated the mechanisms of EMD-induced osteoclast formation using a mous...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2005.00868.x
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:In the oral cavity, the mucosal tissues may develop a number of different pathological conditions, such as inflammatory diseases (gingivitis, periodontitis) and autoimmune disorders (eg, oral lichen planus) that require therapy. The application of topical drugs is one common therapeutic approa...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12487
更新日期:2018-02-01 00:00:00