Abstract:
BACKGROUND AND OBJECTIVE:It remains controversial whether or not the junctional epithelium cells that are directly attached to teeth migrate on the enamel surface, as those cells are able to adhere firmly to the enamel. The aim of this study was to investigate the expression patterns of laminin gamma(2), integrin beta(4) and integrin alpha(3), and to examine their potential function in cell migration. MATERIAL AND METHODS:Oral epithelium cells obtained from Sprague-Dawley rats were established in primary culture. We employed a wound-healing assay to characterize the direction of cell extension at the start of cell migration, and observed different localizations of laminin and integrins using immunofluorescence. For functional analyses of integrins, we employed a phosphatidylinositol-3-kinase (PI3K) activator to promote integrin beta(4) function and used P1B5 to inhibit integrin alpha(3) function, and we analyzed the percentage of re-epithelialization as the migration function. RESULTS:Marked accumulation of laminin gamma(2) was detected in the peripheral cytoplasm of cells adjacent to the wound area, as shown by the results of the migration assay. Integrin beta(4) was detected in the distal cell processes of actively migrating cells, while integrin alpha(3) was found in cell membranes of cells adjacent to the wound area. In the functional analyses, the percentage of re-epithelialization was significantly lower in the PI3K-activator group and in the P1B5-treated group (2.5% and 7.2%, respectively) than in the control group (39.0%) (p < 0.01). CONCLUSION:The results suggest that laminin gamma(2) is secreted as a foothold for cell migration, that integrin beta(4) participates in cell adhesion and that integrin alpha(3) is involved in cell migration in the primary culture cells.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Sugisawa M,Masaoka T,Enokiya Y,Muramatsu T,Hashimoto S,Yamada S,Shimono Mdoi
10.1111/j.1600-0765.2009.01231.xsubject
Has Abstractpub_date
2010-04-01 00:00:00pages
284-91issue
2eissn
0022-3484issn
1600-0765pii
JRE1231journal_volume
45pub_type
杂志文章abstract::The aim of this study was to compare the percentage of vital microorganisms (= microbial vitality) of saliva with that of supragingival plaque both collected at various times during the early phases of de novo plaque formation. Between intervals of optimal oral hygiene, 14 healthy participants refrained from all oral ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb00529.x
更新日期:1997-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:In the oral cavity, the mucosal tissues may develop a number of different pathological conditions, such as inflammatory diseases (gingivitis, periodontitis) and autoimmune disorders (eg, oral lichen planus) that require therapy. The application of topical drugs is one common therapeutic approa...
journal_title:Journal of periodontal research
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更新日期:2018-02-01 00:00:00
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journal_title:Journal of periodontal research
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journal_title:Journal of periodontal research
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doi:10.1111/j.1600-0765.2006.00881.x
更新日期:2006-08-01 00:00:00
abstract::Though cementum of the tooth root is critical for periodontal structure and tooth attachment and function, this tissue was not discovered and characterized on human teeth until a full century later than enamel and dentin. Early observations from the seventeenth to the nineteenth centuries by Marcello Malpighi, Antonie...
journal_title:Journal of periodontal research
pub_type: 历史文章,杂志文章,评审
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更新日期:2017-08-01 00:00:00
abstract::This study uses cytokeratins (CK) as markers to investigate the phenotype of the junctional epithelium (JE) in partially erupted human teeth. The gingival samples, which were clinically healthy, were carefully dissected from the teeth. Cryostat sections were cut for histological staining, immunofluorescence microscopy...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1994.tb01212.x
更新日期:1994-05-01 00:00:00
abstract::Recently, a relationship was demonstrated between the thickness of the cementum layer in rat molars and the activity of alkaline phosphatase (ALP) in the adjoining periodontal ligament (PDL). It was the aim of the present study to investigate whether such a relationship also exists in the periodontium of man. Healthy ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1999.tb02215.x
更新日期:1999-01-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12365
更新日期:2016-12-01 00:00:00
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journal_title:Journal of periodontal research
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journal_title:Journal of periodontal research
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2005.00803.x
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journal_title:Journal of periodontal research
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更新日期:2016-10-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章,评审
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journal_title:Journal of periodontal research
pub_type: 杂志文章
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journal_title:Journal of periodontal research
pub_type: 杂志文章
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更新日期:2018-06-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1990.tb00907.x
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE: The junctional epithelium provides the front-line defense against periodontal bacterial infection. The migration of neutrophils into the junctional epithelium might represent a protective reaction against bacterial infections. However, neutrophils penetrate into the junctional epithelium even...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2012.01490.x
更新日期:2012-12-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12075
更新日期:2014-02-01 00:00:00
abstract::An accumulation of elevated numbers of macrophages (M phi) and Ig producing cells is associated with localized and chronically inflamed gingiva of patients with adult periodontitis. When gingival lymphocytes were isolated from inflamed tissues and examined by flow cytometry, approximately 20-30% of lymphocytes were CD...
journal_title:Journal of periodontal research
pub_type: 杂志文章
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journal_title:Journal of periodontal research
pub_type: 杂志文章
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2007.01081.x
更新日期:2009-02-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1994.tb01100.x
更新日期:1994-03-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
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更新日期:2017-02-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1995.tb02129.x
更新日期:1995-07-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1992.tb01746.x
更新日期:1992-11-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2002.01375.x
更新日期:2002-10-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2012.01501.x
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Periodontal ligament stem cells (PDLSCs) have potential for osteogenic differentiation and show a great foreground in treating bone diseases. Histone three lysine 27 (H3K27) demethylase lysine demethylase 6A (KDM6A) is a critical epigenetic modifier and plays an important role in regulating ost...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12830
更新日期:2020-12-24 00:00:00