Abstract:
BACKGROUND AND OBJECTIVE:Regulation of epithelial cell behavior associated with periodontitis is not well elucidated but many responses will ultimately be regulated by growth factor receptors. Using a rat experimental periodontitis model, protein and gene expression of select growth factor receptors in junctional and pocket epithelium were examined. MATERIAL AND METHODS:Periodontal disease was induced by daily topical application of lipopolysaccharide using an established protocol. Animals were killed at time 0 (control), and at 2 and 8 wk. Frozen tissue samples were collected from the right palatal gingival soft tissue, and the left periodontal tissues were decalcified and embedded in paraffin. Laser microdissection and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to quantify keratinocyte growth factor receptor (KGFR), hepatocyte growth factor receptor (HGFR), epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor 1 (FGFR1) gene expression, and in situ RT-PCR localized these increases to specific epithelial cells. Receptor protein expression was examined immunohistochemically. In cell culture, induction of HGFR and KGFR protein expression by serum, lipopolysaccharide and pro-inflammatory cytokines were examined using flow cytometry. RESULTS:Eight-week tissue samples exhibited histological changes consistent with periodontitis. KGFR and HGFR gene and protein expression were significantly induced at the 8 wk time point. KGFR expression was significantly up-regulated in basal and parabasal pocket epithelial cells, but HGFR was up-regulated throughout the pocket epithelium. In cell culture serum, lipopolysaccharide and pro-inflammatory cytokines, interleukin-1beta and tumour necrosis factor-alpha significantly induced KGFR protein receptor expression, but HGFR expression was only induced by serum. CONCLUSION:KGFR and HGFR are highly up-regulated in this model of periodontal disease and may play a significant role in regulating the proliferation and migration of pocket epithelium.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Ekuni D,Firth JD,Putnins EEdoi
10.1111/j.1600-0765.2006.00881.xsubject
Has Abstractpub_date
2006-08-01 00:00:00pages
340-9issue
4eissn
0022-3484issn
1600-0765pii
JRE881journal_volume
41pub_type
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journal_title:Journal of periodontal research
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doi:10.1111/j.1600-0765.1999.tb02253.x
更新日期:1999-07-01 00:00:00
abstract:OBJECTIVE:Smoking causes pathological changes in all tissues, including gingiva and alveolar bone. The aim of present study was to evaluate apoptotic tissue alterations and tissue destruction in smoker and non-smoker periodontitis patients and healthy individuals. METHODS:Gingival biopsy samples from 15 systemically a...
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journal_title:Journal of periodontal research
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doi:10.1111/j.1600-0765.1999.tb02279.x
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abstract::Accumulating evidence indicates that TNFalpha plays an important role in the pathogenesis of periodontitis, but the effect of TNFalpha on the degradation of the periodontal ligament is not well understood. This study used reverse transcriptase-PCR to investigate the effects of TNFalpha on matrix metalloproteinase (MMP...
journal_title:Journal of periodontal research
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abstract::Effects of lathyrogens such as aminoacetonitrile (AAN), beta-aminopropionitrile (BAPN) and cysteamine--known inhibitors of cross-linking of collagen--on the mechanical strength of the periodontal ligament of the rat mandibular first molar were examined by measuring the ultimate load required to extract the tooth from ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1989.tb00880.x
更新日期:1989-09-01 00:00:00
abstract:OBJECTIVES:Chronic periodontitis is a bone destructive inflammatory disease with an adverse impact on general health and suggested underlying factors in common with osteoporosis. A few studies have examined the possible relationship between chronic periodontitis and osteoporosis; however, the results remain inconclusiv...
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abstract:BACKGROUND AND OBJECTIVE:Periodontitis may alter the systemic condition in patients with diabetes and hence interfere with glycemic control. The objective of this study was to determine the quantifiable changes in glycated hemoglobin (HbA1C) after periodontal non-surgical therapy plus azithromycin in a mixed population...
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abstract::The adherence of Actinomyces naeslundii to human buccal mucosa is mediated by specific interactions between the bacterial cell surface fimbriae and complementary beta-linked galactoside receptors on the epithelial cell surface. The buccal mucosa and the bacteria that colonize its surface are constantly bathed in saliv...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1991.tb01632.x
更新日期:1991-03-01 00:00:00
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journal_title:Journal of periodontal research
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journal_title:Journal of periodontal research
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更新日期:2010-10-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2000.035006369.x
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abstract::Collagenolysis in periodontitis is thought to be modulated by the expression of three genes, collagenase, tissue inhibitors of metalloproteinases-1 and -2 (TIMP-1 and -2). We assessed the possible difference in TIMP-1, TIMP-2 and collagenase mRNA levels between gingival samples from patients with periodontitis and tho...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1993.tb01079.x
更新日期:1993-09-01 00:00:00
abstract::In general, randomized clinical trials (RCT) in dentistry involve longitudinal observations. In such studies, the total cost is a function of the number of study subjects and visits, the study duration, and the type and number of examinations at each visit. In this paper, we derived the minimum cost design for longitu...
journal_title:Journal of periodontal research
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doi:10.1111/j.1600-0765.1999.tb02233.x
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abstract::This study was designed to study wound strength at the dentine/connective tissue interface and at the bone/connective tissue interface following full thickness flap surgery. Flaps of uniform dimension were outlined in four young adult beagle dogs using a standardised double bladed knife and vertical incisions 10 mm ap...
journal_title:Journal of periodontal research
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doi:10.1034/j.1600-0765.2002.01375.x
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journal_title:Journal of periodontal research
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更新日期:2011-02-01 00:00:00
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journal_title:Journal of periodontal research
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更新日期:2020-10-01 00:00:00
abstract::We investigated the co-localization of lysosomal cathepsins B, H and L, and horseradish peroxidase (HRP) in junctional epithelial (JE) cells both as a fluid-phase endocytotic marker to demonstrate the fluid-phase endocytotic capacity of JE cells, and to understand the morphological relationships of the endocytosed for...
journal_title:Journal of periodontal research
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abstract:BACKGROUND AND OBJECTIVES:The expression of A Disintegrin and Metalloproteinase 8 (ADAM8) is associated with several inflammatory diseases. Elevated ADAM8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM8 expression in chroni...
journal_title:Journal of periodontal research
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更新日期:2017-06-01 00:00:00
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journal_title:Journal of periodontal research
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更新日期:2017-12-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb01401.x
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journal_title:Journal of periodontal research
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更新日期:2017-06-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1999.tb02215.x
更新日期:1999-01-01 00:00:00
abstract::The exact cell type and site(s) involved in interleukin-1 (IL-1) production during gingival inflammation was determined by combining immunohistochemistry and in situ hybridization. IL-1 messenger RNA (mRNA)-expressing cells in human inflamed gingiva were identified as macrophages. The rate of IL-alpha mRNA expression ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1993.tb01048.x
更新日期:1993-01-01 00:00:00
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journal_title:Journal of periodontal research
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1989.tb02009.x
更新日期:1989-05-01 00:00:00
abstract::Regeneration of cementum, ligament, bone and new attachment was achieved by introducing mixtures of recombinant human platelet-derived growth factor and insulin-like growth factor into debrided lesions of experimentally induced periodontitis in monkeys. This growth factor therapeutic regimen induced the regeneration o...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1992.tb01679.x
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Porphyromonas gingivalis has been shown to actively invade endothelial cells and induce vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) overexpression. Nucleotide-binding oligomerization domain 1 (NOD1) is an intracellular pattern recognition reporter, ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00