Abstract:
BACKGROUND AND OBJECTIVES:Retinoic acid (RA) exerts a wide variety of effects on development, cellular differentiation and homeostasis in various tissues. However, little is known about the effects of RA on the differentiation of periodontal ligament cells. In this study, we investigated whether RA can affect the dexamethasone-induced differentiation of periodontal ligament cells. METHODS AND RESULTS:Human periodontal ligament cells were differentiated via culturing in the presence of dexamethasone, ascorbic acid, and beta-glycerophosphate for mineralized nodule formation, as characterized by von Kossa staining. Continuous treatment with all-trans-RA inhibited the mineralization in a dose-dependent manner, with complete inhibition over 1 microm RA. Other RA analogs, 9-cis-RA and 13-cis-RA, were also effective. Furthermore, addition of RA for just the first 4 days completely inhibited the mineralization; however, as RA was added at later stages of culture, the inhibitory effect was diminished, suggesting that RA had a phase-dependent inhibition of mineralization. RA receptor (RAR)-alpha agonist (AM-580), but not retinoid X receptor agonist (methoprene acid), inhibited the mineralization, and reverse transcription-polymerase chain reaction analysis revealed that RAR-alpha was expressed on the cells, suggesting that RAR-alpha was involved in the inhibitory mechanism. This inhibition was accompanied by inhibition of alkaline phosphatase activity; however, neither expression of platelet-derived growth factor (PDGF) receptor-alpha, PDGF receptor-beta, or epidermal growth factor (EGF) receptor, nor phosphorylation of extracellular signal-regulated kinases triggered by PDGF-ascorbic acid or PDGF-BB was changed, as assessed by flow cytometry or western blot analyses. CONCLUSIONS:These findings suggest that RA is a potential negative regulator for differentiation of human periodontal ligament cells.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Shibuya N,Nemoto E,Kanaya S,Kunii R,Shimauchi Hdoi
10.1111/j.1600-0765.2005.00811.xsubject
Has Abstractpub_date
2005-12-01 00:00:00pages
432-40issue
6eissn
0022-3484issn
1600-0765pii
JRE811journal_volume
40pub_type
杂志文章abstract::The cytolethal distending toxin (Cdt) of Actinobacillus actinomycetemcomitans, a periodontal pathogen, is a newly described cytotoxin with immunosuppressive properties, capable of causing cell cycle arrest of lymphocytes. The objectives of this study were to investigate the occurrence of A. actinomycetemcomitans with ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2002.01618.x
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The aim of this study was to investigate reactions of periodontal ligament epithelial cell clusters and major histocompatibility complex class II (OX6)-immunopositive cells to simultaneously induced tooth movement and periodontitis employing Waldo's method. MATERIAL AND METHODS:Elastic gums we...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01376.x
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:Reactive oxygen species (ROS) are implicated in the destruction of the periodontium during inflammatory periodontal diseases. The imbalance in oxidant/antioxidant activity may be a key factor in the damaging effects of ROS. This study aimed to determine the lipid peroxidation levels in gingival crevicular fl...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2005.00818.x
更新日期:2005-10-01 00:00:00
abstract::The formation of new cementum is an important issue in clinical periodontology, as cementum is required to provide attachment for newly formed periodontal tissues to the root surface. In this study a model of cementogenesis in vitro was used in order to test the effects of root surface demineralization on the migratio...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb00546.x
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:For ethical reasons it is becoming increasingly more difficult to obtain, from clinical studies, histological data on infrabony defects treated with guided tissue regeneration (GTR) techniques. The aim of this systematic review was to find the value of extrapolating animal data on treatment of ...
journal_title:Journal of periodontal research
pub_type: 杂志文章,meta分析,评审
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更新日期:2014-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Neutrophil extracellular traps (NETs) are a recently discovered antimicrobial mechanism used by neutrophils that have been proposed as an intervention in the pathogenesis of periodontitis. The objective of our study was to characterize the expression of NETs in gingival tissues with periodontit...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12621
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Fibronectin (FN) is an important cell adhesion molecule that is used widely to characterize cell behavior. Preparations of FN purified from human plasma by gelatin-Sepharose affinity chromatography typically also contain gelatin-binding gelatinases that may cleave FN, reduce its stability and a...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2009.01241.x
更新日期:2010-04-01 00:00:00
abstract::The stepwise approach to the determination of periodontal attachment loss involves fitting linear, logarithmic, and exponential models to individual site data and concluding that the form of loss is consistent with the model that has the greatest r-value, provided that the model predicts loss in excess of a site-speci...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1996.tb00458.x
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Metabolic syndrome (MetS) is a combination of risk factors (e.g. impaired glucose tolerance, hypertension, and dyslipidaemia) that significantly contribute to the development of cardiovascular diseases. The aim of the study was to compare the effects of nonsurgical periodontal treatment (NSPT) ...
journal_title:Journal of periodontal research
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doi:10.1111/jre.12328
更新日期:2016-08-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12186
更新日期:2015-02-01 00:00:00
abstract::The exact cell type and site(s) involved in interleukin-1 (IL-1) production during gingival inflammation was determined by combining immunohistochemistry and in situ hybridization. IL-1 messenger RNA (mRNA)-expressing cells in human inflamed gingiva were identified as macrophages. The rate of IL-alpha mRNA expression ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1993.tb01048.x
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Gingival crevicular fluid has been of major interest for many decades as a valuable body fluid that may serve as a source of biomarkers for both periodontal and systemic diseases. Owing to its very small sample size, submicroliter volumes, identification of its protein composition by classical ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01429.x
更新日期:2012-04-01 00:00:00
abstract::Quantitative assessment of osseous changes attributable to periodontal disease is made possible by digital subtraction radiography. Tissues through which x rays travel to produce dental radiographs essential to this process alter the energy spectrum of the beam such that calibration errors result when densitometry is ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1989.tb00857.x
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Porphyromonas gingivalis has been shown to actively invade endothelial cells and induce vascular cell adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) overexpression. Nucleotide-binding oligomerization domain 1 (NOD1) is an intracellular pattern recognition reporter, ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12192
更新日期:2015-04-01 00:00:00
abstract::Materials used in guided tissue regeneration were examined in an in vitro model for regenerating human junctional epithelium. The extent of epithelial migration from gingival explants (epibolus) between the explant and an experimental substratum was significantly greater with a Millipore filter (HA) than with a teflon...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1990.tb00907.x
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Cannabinoid receptor CB2, expressed in osteoblasts and osteoclasts, plays a crucial role in the regulation of bone metabolism. Since periodontal ligament (PDL) cells can differentiate into osteoblasts, this study was undertaken to investigate CB2 expression and the effect of CB2 activation on o...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2009.01265.x
更新日期:2010-08-01 00:00:00
abstract::Four rough-surfaced (R) and three smooth-surfaced (S) clinical isolates of Capnocytophaga obtained from the subgingival plaque of periodontitis patients were studied for their peptidase and protease profiles. The results were compared with those obtained with C. gingivalis (which has a smooth morphology). All cell ext...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1991.tb01621.x
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:As epithelial cells function as a mechanical barrier, the permeability of the gingival epithelial cell layer indicates a defensive capability against invasion by periodontal pathogens. We have reported the expression of claudin-1 and E-cadherin, key regulators of permeability, in the gingival j...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01404.x
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Membrane type 1-matrix metalloproteinase (MT1-MMP) is a collagenolytic enzyme involved in connective tissue remodeling. In periodontal tissues, either cytokines or growth factors regulate the production of proteolytic enzymes. Mice deficient in epidermal growth factor receptor (EGFR) show a re...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2007.01081.x
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The gingiva is the first oral tissue directly exposed to cigarette smoke (CS). Exposure to CS compromises the structure and function of gingival tissue. Damaging or altering the gingival epithelium leads to a compromised protective barrier of the periodontium, resulting in several diseases. The...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12343
更新日期:2016-10-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1997.tb00529.x
更新日期:1997-02-01 00:00:00
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journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12535
更新日期:2018-06-01 00:00:00
abstract::To document the effect of hypophysectomy and growth hormone replacement on the ultrastructure of cementogenesis in the developing rat third molar, 12 female Wistar rats were randomly allocated to normal control, hypophysectomized or hypophysectomized plus human growth hormone (for 10 days) treatment groups. The result...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1994.tb01221.x
更新日期:1994-07-01 00:00:00
abstract::The localization of desmoplakins 1 and 2 (DP 1&2), components of desmosomes, vinculin, and actin, was studied in gingival epithelial cells grown on cell culture glass and on titanium plates with various surface topography. The results showed that epithelial cells attached and spread more readily on smooth than on roug...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1991.tb01800.x
更新日期:1991-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Cigarette smoking is a major risk factor for periodontitis, and smoking perturbs neutrophil reactive oxygen species production. This study tested the hypothesis that cigarette smoke extract (CSE) and its components/metabolites nicotine, cotinine and thiocyanate (SCN-), may influence neutrophil ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12542
更新日期:2018-08-01 00:00:00
abstract::Colonization or emergence of microbial pathogens may result in tissue destruction by activation of one or more of five distinct host degradative pathways (matrix metalloproteinase pathway, plasminogen-dependent pathway, phagocytic pathway, PMN-serine proteinase pathway and osteoclastic bone resorption) or by direct cl...
journal_title:Journal of periodontal research
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0765.1993.tb02113.x
更新日期:1993-11-01 00:00:00
abstract::The 3 human salivary cystatins S, SA and SN are multifunctional proteins that possess a cysteine protease inhibitory property, but their ability to act as such is very different (SN > SA > S). One form, S, also appears to possess antibacterial properties towards the bacterium Porphyromonas gingivalis, often associated...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1999.tb02279.x
更新日期:1999-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Cyclosporine A treatment is important in the therapy of a number of medical conditions; however, alveolar bone loss is an important negative side-effect of this drug. As such, we evaluated whether concomitant administration of simvastatin would minimize cyclosporine A-associated alveolar bone l...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2008.01143.x
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVE:The aim of this immunocytochemical study was to characterize the expression and distribution of the progesterone receptor (PR) and estrogen receptor (ER) in gingival fibroblasts using culture cells derived from people at various ages. BACKGROUND:The reaction of female hormones is tissue or cell specific, and...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2003.00654.x
更新日期:2003-06-01 00:00:00
abstract::Vascularization of the periodontal ligament was examined in developing upper first molars of rats from 5 to 30 d after birth with light and scanning electron microscopy. Formation of the vascular network in the periodontal ligament (PDL) started with the beginning of root formation. The PDL vessels derived from the ba...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2000.035004201.x
更新日期:2000-08-01 00:00:00