当前位置： SCI文献检索 > JOURNAL OF PERIODONTAL RESEARCH期刊下所有文献 > Expression and intracellular localization of progesterone receptors in cultured human gingival fibroblasts.

Expression and intracellular localization of progesterone receptors in cultured human gingival fibroblasts.

Abstract：

OBJECTIVE:The aim of this immunocytochemical study was to characterize the expression and distribution of the progesterone receptor (PR) and estrogen receptor (ER) in gingival fibroblasts using culture cells derived from people at various ages. BACKGROUND:The reaction of female hormones is tissue or cell specific, and receptor availability in the cell is one of the major causes for the different reactions. Gingiva is a target tissue for female hormones; however, the characteristics of PR and ER in both the fibroblasts and the other component cells remain largely unknown. MATERIALS:Gingival tissue was obtained from six people at various ages and culture fibroblasts were established. At least three passages of each cell line were strained for PR and ER with monoclonal antibodies (Clone 1A6, Clone 1D5, respectively). RESULTS:PR positive cells were detected in all six cell lines through early passages to late ones, but ER were only observed in two of six samples with faint reactions. The staining intensity for PR was greater than for ER, but less than that shown in the MCF-7 breast cancer cells, positive control. In every positive control test, ER reactivity was equal to or higher than that of PR. During the interphase, significantly fewer positive fibroblasts occurred compared with negative fibroblasts, and positive nuclei were even fewer. Meanwhile, most of the mitotic cells were PR positive, showing intense localization around chromosomes and on microtubules. These findings suggest that gingival fibroblasts are fundamentally capable of expressing PR and transmit the signal to target genes. CONCLUSIONS:The present study may conclude that in either gender or at any age, gingival fibroblasts express PR rather low in level and do not necessarily localize PR in a nuclear dominant fashion, which is an essential feature for reproductive organ cells. The poor ER reactivity shown in the gingival fibroblasts was discussed in view of the receptor subtype.

journal_name

J Periodontal Res

journal_title

Journal of periodontal research

authors

Kawahara K,Shimazu A

doi

10.1034/j.1600-0765.2003.00654.x

subject

Has Abstract

pub_date

2003-06-01 00:00:00

pages

242-6

issue

3

eissn

0022-3484

issn

1600-0765

pii

654

journal_volume

38

pub_type

杂志文章

相关文献

JOURNAL OF PERIODONTAL RESEARCH文献大全
  • Significance of elevated gingival crevicular fluid tumor necrosis factor-alpha and interleukin-8 levels in chronic hemodialysis patients with periodontal disease.

    abstract：BACKGROUND AND OBJECTIVE:The prevalence of chronic renal disease in industrialized countries is increasing, and chronic renal disease and periodontitis can have significant, reciprocal effects. The aim of this study was to evaluate the associations between specific clinical parameters and the levels of tumor necrosis f...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2009.01252.x

    authors： Dağ A,Firat ET,Kadiroğlu AK,Kale E,Yilmaz ME

    更新日期：2010-08-01 00:00:00

  • Effects of TNFalpha and prostaglandin E2 on the expression of MMPs in human periodontal ligament fibroblasts.

    abstract：:Accumulating evidence indicates that TNFalpha plays an important role in the pathogenesis of periodontitis, but the effect of TNFalpha on the degradation of the periodontal ligament is not well understood. This study used reverse transcriptase-PCR to investigate the effects of TNFalpha on matrix metalloproteinase (MMP...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1034/j.1600-0765.2002.00656.x

    authors： Nishikawa M,Yamaguchi Y,Yoshitake K,Saeki Y

    更新日期：2002-06-01 00:00:00

  • Immunolocalization of proteins specific for adhaerens junctions in human gingival epithelial cells grown on differently processed titanium surfaces.

    abstract：:The localization of desmoplakins 1 and 2 (DP 1&2), components of desmosomes, vinculin, and actin, was studied in gingival epithelial cells grown on cell culture glass and on titanium plates with various surface topography. The results showed that epithelial cells attached and spread more readily on smooth than on roug...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1991.tb01800.x

    authors： Hormia M,Könönen M,Kivilahti J,Virtanen I

    更新日期：1991-11-01 00:00:00

  • Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277.

    abstract：:The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcomitans, Porphyromonas ging...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1996.tb01412.x

    authors： Johansson A,Bergenholtz A,Holm SE

    更新日期：1996-10-01 00:00:00

  • Role of cytokines and inflammatory mediators in tissue destruction.

    abstract：:Colonization or emergence of microbial pathogens may result in tissue destruction by activation of one or more of five distinct host degradative pathways (matrix metalloproteinase pathway, plasminogen-dependent pathway, phagocytic pathway, PMN-serine proteinase pathway and osteoclastic bone resorption) or by direct cl...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章,评审

    doi：10.1111/j.1600-0765.1993.tb02113.x

    authors： Birkedal-Hansen H

    更新日期：1993-11-01 00:00:00

  • Attachment, proliferation and differentiation of periodontal ligament cells on various guided tissue regeneration membranes.

    abstract：:The purpose of this study was to evaluate the biological effects of guided tissue regeneration (GTR) membrane materials, per se, on the periodontal tissue regeneration. Rat periodontal ligament (PDL)-derived cells were used to study the attachment, proliferation and differentiation, in vitro, on various GTR membranes....

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1034/j.1600-0765.2001.360508.x

    authors： Takata T,Wang HL,Miyauchi M

    更新日期：2001-10-01 00:00:00

  • A tumor necrosis factor-alpha antagonist inhibits inflammatory bone resorption induced by Porphyromonas gingivalis infection in mice.

    abstract：BACKGROUND:A tumor-necrosis factor-alpha (TNF-alpha) antagonist, the WP9QY peptide, was designed based on the crystal structure of the TNF-beta/TNF-receptor complex in order to overcome the disadvantages of macromolecules such as antibodies or soluble receptors by reducing the molecular size of TNF-alpha antagonists. I...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2005.00812.x

    authors： Suzuki Y,Aoki K,Saito H,Umeda M,Nitta H,Baron R,Ohya K

    更新日期：2006-04-01 00:00:00

  • Vital microorganisms in early supragingival dental plaque and in stimulated human saliva.

    abstract：:The aim of this study was to compare the percentage of vital microorganisms (= microbial vitality) of saliva with that of supragingival plaque both collected at various times during the early phases of de novo plaque formation. Between intervals of optimal oral hygiene, 14 healthy participants refrained from all oral ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1997.tb00529.x

    authors： Weiger R,von Ohle C,Decker E,Axmann-Krcmar D,Netuschil L

    更新日期：1997-02-01 00:00:00

  • Tetracycline release from tripolyphosphate-chitosan cross-linked sponge: a preliminary in vitro study.

    abstract：BACKGROUND AND OBJECTIVE:The aim of this study was to design a tripolyphosphate-chitosan cross-linked tetracycline-containing (TPP-TC) sponge that slowly releases tetracycline, for future periodontal applications. MATERIAL AND METHODS:Chitosan sponge was made by freezing and drying 2.5% chitosan solution. Tripolyphosp...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2007.01045.x

    authors： Shen EC,Wang C,Fu E,Chiang CY,Chen TT,Nieh S

    更新日期：2008-12-01 00:00:00

  • Lipopolysaccharide-regulated production of bone sialoprotein and interleukin-8 in human periodontal ligament fibroblasts: the role of toll-like receptors 2 and 4 and the MAPK pathway.

    abstract：BACKGROUND AND OBJECTIVE:Lipopolysaccharide (LPS) on the cell wall of periodontal pathogens is a major mediator of the inflammatory response and can enhance alveolar bone resorption in periodontitis. Bone sialoprotein is an early marker of osteoblast differentiation. The proinflammatory cytokine, interleukin-8 (IL-8), ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12193

    authors： Zhang Y,Li X

    更新日期：2015-04-01 00:00:00

  • Effect of 1,25(OH)2 D3 and 20(OH)D3 on interleukin-1β-stimulated interleukin-6 and -8 production by human gingival fibroblasts.

    abstract：BACKGROUND AND OBJECTIVE:Vitamin D-1,25(OH)2 D3 or 1,25D3-maintains healthy osseous tissue, stimulates the production of the antimicrobial peptide cathelicidin and has anti-inflammatory effects, but it can cause hypercalcemia. Evidence links diminished serum levels of 1,25D3 with increased gingival inflammation. Period...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12452

    authors： Nakashyan V,Tipton DA,Karydis A,Livada R,Stein SH

    更新日期：2017-10-01 00:00:00

  • Roxithromycin inhibits tumor necrosis factor-alpha-induced matrix metalloproteinase-1 expression through regulating mitogen-activated protein kinase phosphorylation and Ets-1 expression.

    abstract：BACKGROUND AND OBJECTIVE:In periodontitis, matrix metalloproteinases (MMPs) are upregulated in response to locally released inflammatory cytokines, resulting in pathologic processes. Roxithromycin is a 14-membered ring macrolide antibiotic with broad-spectrum antibacterial effects against oral pathogens and immunomodul...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2006.00914.x

    authors： Oyama T,Matsushita K,Sakuta T,Tokuda M,Tatsuyama S,Nagaoka S,Torii M

    更新日期：2007-02-01 00:00:00

  • Periodontitis as a risk factor for cerebrovascular accident: a case-control study in the Indian population.

    abstract：BACKGROUND AND OBJECTIVE:Chronic infections have been reported to be risk factors for coronary heart disease and ischemic stroke. However, the association of coronary heart disease and ischemic stroke with periodontal disease, which is also a chronic inflammatory disease, needs to be evaluated. The present case-control...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2009.01220.x

    authors： Pradeep AR,Hadge P,Arjun Raju P,Shetty SR,Shareef K,Guruprasad CN

    更新日期：2010-04-01 00:00:00

  • The effect of orally administered probiotic Lactobacillus reuteri-containing tablets in peri-implant mucositis: a double-blind randomized controlled trial.

    abstract：BACKGROUND AND OBJECTIVES:Probiotics create a biofilm and protect the oral tissues against the action of periodontal pathogenic bacteria. The aim of this study was to evaluate the effects of the oral probiotic Lactobacillus reuteri Prodentis upon the peri-implant health of edentulous patients with dental implants and p...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章,随机对照试验

    doi：10.1111/jre.12264

    authors： Flichy-Fernández AJ,Ata-Ali J,Alegre-Domingo T,Candel-Martí E,Ata-Ali F,Palacio JR,Peñarrocha-Diago M

    更新日期：2015-12-01 00:00:00

  • Anti-inflammatory properties of enamel matrix derivative in human blood.

    abstract：BACKGROUND AND OBJECTIVE:Enamel matrix derivative (EMD), extracted from porcine tooth buds, has been shown to promote periodontal healing in patients with severe periodontitis. This involves modulation of the inflammatory response followed by the onset of periodontal regeneration. Based on these observations, we examin...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2005.00863.x

    authors： Myhre AE,Lyngstadaas SP,Dahle MK,Stuestøl JF,Foster SJ,Thiemermann C,Lilleaasen P,Wang JE,Aasen AO

    更新日期：2006-06-01 00:00:00

  • Irsogladine maleate counters the interleukin-1 beta-induced suppression in gap-junctional intercellular communication but does not affect the interleukin-1 beta-induced zonula occludens protein-1 levels in human gingival epithelial cells.

    abstract：BACKGROUND AND OBJECTIVE:Irsogladine maleate counters gap junctional intercellular communication reduction induced by interleukin-8 or Actinobacillus actinomycetemcomitans in cultured human gingival epithelial cells. Interleukin-1 beta is involved in periodontal disease. Little is known, however, about the effect of in...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2007.01000.x

    authors： Fujita T,Ashikaga A,Shiba H,Kajiya M,Kishimoto A,Hirata R,Tsunekuni N,Hirono C,Kawaguchi H,Shiba Y,Kurihara H

    更新日期：2008-02-01 00:00:00

  • Periodontal disease and quality of life: Umbrella review of systematic reviews.

    abstract：:This umbrella review appraised existing systematic reviews and meta-analysis to establish the impact of periodontal disease and therapy on general and oral health-related quality of life. A systematic electronic literature search was carried out in accordance with the PRISMA guideline up to January 2020 using PubMed, ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章,meta分析,评审

    doi：10.1111/jre.12805

    authors： Wong LB,Yap AU,Allen PF

    更新日期：2021-01-01 00:00:00

  • Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells.

    abstract：BACKGROUND AND OBJECTIVES:The expression of A Disintegrin and Metalloproteinase 8 (ADAM8) is associated with several inflammatory diseases. Elevated ADAM8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate ADAM8 expression in chroni...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12426

    authors： Aung WPP,Chotjumlong P,Pata S,Montreekachon P,Supanchart C,Khongkhunthian S,Sastraruji T,Krisanaprakornkit S

    更新日期：2017-06-01 00:00:00

  • Five parameters of gingival crevicular fluid from eight surfaces in periodontal health and disease.

    abstract：:Volume and amounts of myeloperoxidase (MPO), lactoferrin (LF), aryl sulfatase (AS) and lactate dehydrogenase (LDH) were measured in gingival crevicular fluid (GCF) collected from the mesial and distal proximal surfaces of the premolars and first and second molars of 3 subject groups. Group assignment was based on subj...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1992.tb01819.x

    authors： Smith QT,Au GS,Freese PL,Osborn JB,Stoltenberg JL

    更新日期：1992-09-01 00:00:00

  • Effect of bisphosphonates on human gingival fibroblast production of mediators of osteoclastogenesis: RANKL, osteoprotegerin and interleukin-6.

    abstract：BACKGROUND AND OBJECTIVE:Osteonecrosis of the jaw (ONJ) is associated with bisphosphonate (BP) therapy. BPs alter osteoblast production of mediators of osteoclastogenesis, including interleukin (IL)-6, RANKL and osteoprotegerin (OPG), a RANKL antagonist. This can inhibit bone turnover and lead to necrosis. There is lit...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2010.01306.x

    authors： Tipton DA,Seshul BA,Dabbous MKh

    更新日期：2011-02-01 00:00:00

  • Effects of vascular endothelial cells on osteogenic differentiation of noncontact co-cultured periodontal ligament stem cells under hypoxia.

    abstract：BACKGROUND AND OBJECTIVE:During periodontitis or orthodontic tooth movement, the periodontal vasculature is severely impaired by chronic inflammation or excessive mechanical force. This leads to a hypoxic microenvironment of the periodontal cells and enhances the expression of various cytokines and growth factors that ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2012.01503.x

    authors： Wu Y,Cao H,Yang Y,Zhou Y,Gu Y,Zhao X,Zhang Y,Zhao Z,Zhang L,Yin J

    更新日期：2013-02-01 00:00:00

  • Porphyromonas gingivalis displays a competitive advantage over Aggregatibacter actinomycetemcomitans in co-cultured biofilm.

    abstract：BACKGROUND AND OBJECTIVE:Biofilm formation occurs through the events of cooperative growth and competitive survival among multiple species. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans are important periodontal pathogens. The aim of this study was to demonstrate competitive or cooperative interact...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12006

    authors： Takasaki K,Fujise O,Miura M,Hamachi T,Maeda K

    更新日期：2013-06-01 00:00:00

  • Alendronate regulates cytokine production induced by lipid A through nuclear factor-κB and Smad3 activation in human gingival fibroblasts.

    abstract：BACKGROUND AND OBJECTIVE:Nitrogen-containing bisphosphonates (NBPs) are widely used as anti-bone-resorptive drugs. However, use of NBPs results in inflammatory side-effects, including jaw osteomyelitis. In the present study, we examined the effects of alendronate, a typical NBP, on cytokine production by human peripher...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2010.01302.x

    authors： Tamai R,Sugiyama A,Kiyoura Y

    更新日期：2011-02-01 00:00:00

  • From manual periodontal probing to digital 3-D imaging to endoscopic capillaroscopy: Recent advances in periodontal disease diagnosis.

    abstract：:Deepened periodontal pockets exert a significant pathological burden on the host and its immune system, particularly in a patient with generalized moderate to severe periodontitis. This burden is extensive and longitudinal, occurring over decades of disease development. Considerable diagnostic and prognostic successes...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章,评审

    doi：10.1111/jre.12585

    authors： Elashiry M,Meghil MM,Arce RM,Cutler CW

    更新日期：2019-02-01 00:00:00

  • Expression and function of laminin and integrins on adhesion/migration of primary culture cells derived from rat oral epithelium.

    abstract：BACKGROUND AND OBJECTIVE:It remains controversial whether or not the junctional epithelium cells that are directly attached to teeth migrate on the enamel surface, as those cells are able to adhere firmly to the enamel. The aim of this study was to investigate the expression patterns of laminin gamma(2), integrin beta(...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2009.01231.x

    authors： Sugisawa M,Masaoka T,Enokiya Y,Muramatsu T,Hashimoto S,Yamada S,Shimono M

    更新日期：2010-04-01 00:00:00

  • Urokinase-plasminogen activator protects periodontal ligament fibroblast from oxidative induced-apoptosis and DNA damage.

    abstract：BACKGROUND AND OBJECTIVE:Urokinase-plasminogen activator (uPA) is a serine protease expressed at high basal level in normal gingival cervical fluid. Despite its known pathologic role in tissue proteolysis in periodontitis, little is known concerning uPA physiological function in oral tissue. Recent evidence in cancer c...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12576

    authors： Ahmad Akhoundi MS,Rokn A,Bagheri R,Momeni N,Hodjat M

    更新日期：2018-10-01 00:00:00

  • On the discovery of cementum.

    abstract：:Though cementum of the tooth root is critical for periodontal structure and tooth attachment and function, this tissue was not discovered and characterized on human teeth until a full century later than enamel and dentin. Early observations from the seventeenth to the nineteenth centuries by Marcello Malpighi, Antonie...

    journal_title：Journal of periodontal research

    pub_type： 历史文章,杂志文章,评审

    doi：10.1111/jre.12444

    authors： Foster BL

    更新日期：2017-08-01 00:00:00

  • Periodontal conditions among adults in Southern Thailand.

    abstract：OBJECTIVE:To describe the periodontal conditions among 30-39- and 50-59-year-old rural Thais from the Province of Songkhla, Southern Thailand. METHODS:A total of 359 dentate persons were given a clinical examination comprising recordings of plaque and calculus in six teeth, and bleeding on probing, attachment level an...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1034/j.1600-0765.2003.00367.x

    authors： Baelum V,Pisuithanakan S,Teanpaisan R,Pithpornchaiyakul W,Pongpaisal S,Papapanou PN,Dahlén G,Fejerskov O

    更新日期：2003-04-01 00:00:00

  • Isolation and characterization of a 180-kiloDalton salivary glycoprotein which mediates the attachment of Actinomyces naeslundii to human buccal epithelial cells.

    abstract：:The adherence of Actinomyces naeslundii to human buccal mucosa is mediated by specific interactions between the bacterial cell surface fimbriae and complementary beta-linked galactoside receptors on the epithelial cell surface. The buccal mucosa and the bacteria that colonize its surface are constantly bathed in saliv...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1991.tb01632.x

    authors： Babu JP,Dabbous MK,Abraham SN

    更新日期：1991-03-01 00:00:00

  • Gingival crevicular interleukin-1 and interleukin-1 receptor antagonist levels in periodontally healthy and diseased sites.

    abstract：:Interleukin-1 (IL-1) molecules, IL-1 alpha and IL-1 beta are cytokines involved in the acute-phase response against infection and in the pathogenesis of periodontal destruction. Administration of exogenous IL-1 receptor antagonist (IL-1ra) is effective in reducing the inflammatory reactions mediated by IL-1. However, ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1997.tb00568.x

    authors： Ishihara Y,Nishihara T,Kuroyanagi T,Shirozu N,Yamagishi E,Ohguchi M,Koide M,Ueda N,Amano K,Noguchi T

    更新日期：1997-08-01 00:00:00