当前位置： SCI文献检索 > JOURNAL OF PERIODONTAL RESEARCH期刊下所有文献 > Biochemical comparison of proteolytic enzymes present in rough- and smooth-surfaced capnocytophagas isolated from the subgingival plaque of periodontitis patients.

Biochemical comparison of proteolytic enzymes present in rough- and smooth-surfaced capnocytophagas isolated from the subgingival plaque of periodontitis patients.

Abstract：

:Four rough-surfaced (R) and three smooth-surfaced (S) clinical isolates of Capnocytophaga obtained from the subgingival plaque of periodontitis patients were studied for their peptidase and protease profiles. The results were compared with those obtained with C. gingivalis (which has a smooth morphology). All cell extracts obtained by ultrasonic treatment displayed high peptidase activity toward N-aminoacyl-2-naphthylamines, the best substrates being the arginyl, aspartyl, and leucyl derivatives. The R and S isolates did not differ in these enzyme activities. Also the protease profiles studies with 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-proly l-D-arginine (PZ-PLPGA) and casein were similar. All extracts also hydrolyzed furylacryloyl-L-leucylglycyl-L-prolyl-L-alanine (FALGPA), reconstituted type I [3H]-collagen, and gelatin. N alpha-Benzoyl-DL-rginyl-2-naphthylamine was hydrolyzed faster by the R than the S strains. Comparison between cell suspensions and cell extracts of C. gingivalis showed the suspensions to be enzymatically more active than the extracts. In general, peptidase substrates and PZ-PLGPA were hydrolyzed at a higher rate by suspensions than by extracts, while protease substrates (such as casein) were hydrolyzed faster by the extracts. Gelatin and FALGPA were hydrolyzed by cell extracts only. Fast protein liquid chromatography of peptidases on a gel column was found to be a suitable method to differentiate between R and S isolates in diagnostics, while the chromatographic profiles of proteases were not suitable for this purpose.

journal_name

J Periodontal Res

journal_title

Journal of periodontal research

authors

Söderling E,Mäkinen PL,Syed S,Mäkinen KK

doi

10.1111/j.1600-0765.1991.tb01621.x

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

17-23

issue

1

eissn

0022-3484

issn

1600-0765

journal_volume

26

pub_type

杂志文章

相关文献

JOURNAL OF PERIODONTAL RESEARCH文献大全
  • Levels of specific immunoglobulin G to the forsythia detaching factor of Tannerella forsythia in gingival crevicular fluid are related to the periodontal status.

    abstract：BACKGROUND AND OBJECTIVE:Forsythia detaching factor (FDF) is a putative virulence factor of Tannerella forsythia that induces detachment of adherent cells and interleukin-8 production in human fibroblasts. The objective of the present study was to clarify the relationship between anti-FDF IgG levels in gingival crevicu...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2010.01283.x

    authors： Onishi H,Arakawa S,Nakajima T,Izumi Y

    更新日期：2010-10-01 00:00:00

  • Isolation and characterization of a 180-kiloDalton salivary glycoprotein which mediates the attachment of Actinomyces naeslundii to human buccal epithelial cells.

    abstract：:The adherence of Actinomyces naeslundii to human buccal mucosa is mediated by specific interactions between the bacterial cell surface fimbriae and complementary beta-linked galactoside receptors on the epithelial cell surface. The buccal mucosa and the bacteria that colonize its surface are constantly bathed in saliv...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1991.tb01632.x

    authors： Babu JP,Dabbous MK,Abraham SN

    更新日期：1991-03-01 00:00:00

  • Alendronate regulates cytokine production induced by lipid A through nuclear factor-κB and Smad3 activation in human gingival fibroblasts.

    abstract：BACKGROUND AND OBJECTIVE:Nitrogen-containing bisphosphonates (NBPs) are widely used as anti-bone-resorptive drugs. However, use of NBPs results in inflammatory side-effects, including jaw osteomyelitis. In the present study, we examined the effects of alendronate, a typical NBP, on cytokine production by human peripher...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2010.01302.x

    authors： Tamai R,Sugiyama A,Kiyoura Y

    更新日期：2011-02-01 00:00:00

  • Electron-immunocytochemistry of laminin and type-IV collagen in the junctional epithelium of rat molar gingiva.

    abstract：:The present study was carried out to examine the ultrastructural localization of laminin and type-IV collagen in the junctional epithelium of rat molar gingiva by means of the indirect immunoperoxidase method. Intense laminin reaction occurred in both the internal and the external basal laminae. Laminin reaction produ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1990.tb00930.x

    authors： Sawada T,Yamamoto T,Yanagisawa T,Takuma S,Hasegawa H,Watanabe K

    更新日期：1990-11-01 00:00:00

  • Mechanisms involved in regulation of periodontal ligament cell production of pro-inflammatory cytokines: Implications in periodontitis.

    abstract：:It is well recognized that human periodontal ligament cells (PDL cells) may represent local immune cells of the periodontal tissues. However, it is unclear whether they represent "true" immune cells, since they can produce pro-inflammatory cytokines not only after stimulation with bacterial lipopolysaccharides but als...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12823

    authors： Nilsson BO

    更新日期：2020-12-10 00:00:00

  • Factors affecting human supragingival biofilm composition. I. Plaque mass.

    abstract：BACKGROUND AND OBJECTIVE:Little is known about the factors that affect the microbial composition of supragingival biofilms. This study was designed to examine the relationship between total DNA probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition. MATERIAL AND METHOD...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2008.01154.x

    authors： Haffajee AD,Teles RP,Patel MR,Song X,Veiga N,Socransky SS

    更新日期：2009-08-01 00:00:00

  • Chemical sympathectomy inhibits periodontal disease in Fischer 344 rats.

    abstract：OBJECTIVE:The responsiveness of the sympathetic nervous system (SNS) and the hypothalamic--pituitary--adrenal (HPA) axis plays a major role in immune regulation and for the outcome of infections and inflammatory disorders. This study was designed to investigate whether chemical SNS denervation with the noradrenaline-se...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2005.00803.x

    authors： Breivik T,Gundersen Y,Opstad PK,Fonnum F

    更新日期：2005-08-01 00:00:00

  • Characterization of hyperbranched core-multishell nanocarriers as an innovative drug delivery system for the application at the oral mucosa.

    abstract：BACKGROUND AND OBJECTIVES:In the oral cavity, the mucosal tissues may develop a number of different pathological conditions, such as inflammatory diseases (gingivitis, periodontitis) and autoimmune disorders (eg, oral lichen planus) that require therapy. The application of topical drugs is one common therapeutic approa...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12487

    authors： Jager J,Obst K,Lohan SB,Viktorov J,Staufenbiel S,Renz H,Unbehauen M,Haag R,Hedtrich S,Teutloff C,Meinke MC,Danker K,Dommisch H

    更新日期：2018-02-01 00:00:00

  • Strong cytotoxicity to human gingival fibroblasts by Porphyromonas gingivalis ATCC 33277.

    abstract：:The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcomitans, Porphyromonas ging...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1996.tb01412.x

    authors： Johansson A,Bergenholtz A,Holm SE

    更新日期：1996-10-01 00:00:00

  • Enhanced collagen phagocytosis by rat molar periodontal fibroblasts after topical application of lipopolysaccharide--ultrastructural observations and morphometric analysis.

    abstract：:To investigate the effect of lipopolysaccharide (LPS) on phagocytic activity of collagen fibrils by periodontal fibroblasts, we studied rat molar gingival connective tissue and periodontal ligament under light and electron microscopy after topical application of LPS (5 mg/ml in physiological salt solution (PS)) on the...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1992.tb01665.x

    authors： Ijuhin N,Miyauchi M,Ito H,Takata T,Ogawa I,Nikai H

    更新日期：1992-05-01 00:00:00

  • Periodontal disease and percentage of calories from fat using national data.

    abstract：BACKGROUND AND OBJECTIVE:The association between periodontal disease and nutrient intake was examined using linked data from the 2005 National Health and Nutrition Survey, the Comprehensive Survey of Living Conditions and the Survey of Dental Diseases from the same year 'using linked data from the National Health and N...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12375

    authors： Hamasaki T,Kitamura M,Kawashita Y,Ando Y,Saito T

    更新日期：2017-02-01 00:00:00

  • Alpha-terpineol complexed with beta-cyclodextrin reduces damages caused by periodontitis in rats.

    abstract：BACKGROUND AND OBJECTIVE:This study aimed to assess the effectiveness of the treatment with alpha-terpineol (αTPN) complexed with beta-cyclodextrin (βCD) on oral, blood, and hepatic parameters in ligature-induced periodontitis. MATERIAL AND METHODS:Forty female rats were distributed among the following groups: control...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12780

    authors： Vasconcelos ACCG,Vasconcelos DFP,da Silva FRP,França LFC,Alves EHP,Di Lenardo D,Pessoa LDS,Nascimento HMS,Carvalho ADS,Sousa FBM,Barbosa ALDR,Medeiros JR,Novaes PD,Mariano FS,Lima BDS,Araujo AAS,Júnior LJQ,de Oliveira A

    更新日期：2020-12-01 00:00:00

  • Variance components of gingival thickness.

    abstract：OBJECTIVES:Distinct periodontal phenotypes have been identified by cluster analysis, which is an explorative method with very low external validity. The aim of the present study was to investigate variance components of facial gingival thickness in young adults with mild gingivitis. MATERIAL AND METHODS:Thirty-three n...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2005.00798.x

    authors： Müller HP,Könönen E

    更新日期：2005-06-01 00:00:00

  • On the discovery of cementum.

    abstract：:Though cementum of the tooth root is critical for periodontal structure and tooth attachment and function, this tissue was not discovered and characterized on human teeth until a full century later than enamel and dentin. Early observations from the seventeenth to the nineteenth centuries by Marcello Malpighi, Antonie...

    journal_title：Journal of periodontal research

    pub_type： 历史文章,杂志文章,评审

    doi：10.1111/jre.12444

    authors： Foster BL

    更新日期：2017-08-01 00:00:00

  • Interleukin-8 and anti-interleukin-8 autoantibodies in gingival crevicular fluid from patients with periodontitis.

    abstract：:Interleukin-8 (IL-8) is believed to play an important role in the pathogenesis of various forms of periodontitis. In addition, the anti-IL-8 autoantibody has been recently recognized as a potent modulator of IL-8 function. In the current study, the concentrations of IL-8 and its autoantibody in gingival crevicular flu...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1034/j.1600-0765.2003.02001.x

    authors： Kurdowska AK,Noble JM,Adcock JE

    更新日期：2003-02-01 00:00:00

  • Bradykinin-2 receptor-mediated release of 3H-arachidonic acid and formation of prostaglandin E2 in human gingival fibroblasts.

    abstract：:Bradykinin stimulated production of prostaglandin E2 (PGE2) and the release of 3H-arachidonic acid by gingival fibroblasts in a time- and dose-dependent manner. The effect on PGE2 biosynthesis was seen already after 15 seconds and was maximal after 5 minutes. Several structurally unrelated inhibitors of arachidonic ac...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1990.tb00928.x

    authors： Modéer T,Ljunggren O,Lerner UH

    更新日期：1990-11-01 00:00:00

  • Patterns of cytokeratin expression in monkey and human periodontium following regenerative and conventional periodontal surgery.

    abstract：:The pattern of cytokeratin expression has been extensively described in the normal and inflamed periodontium. However, there is no information regarding the pattern of cytokeratin expression in the periodontium which has been reformed following regenerative periodontal surgery. The aim of the present investigation was...

    journal_title：Journal of periodontal research

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1034/j.1600-0765.2001.036004260.x

    authors： Sculean A,Berakdar M,Pahl S,Windisch P,Brecx M,Reich E,Donos N

    更新日期：2001-08-01 00:00:00

  • Activated KCNQ1 channel promotes fibrogenic response in hereditary gingival fibromatosis via clustering and activation of Ras.

    abstract：BACKGROUND AND OBJECTIVE:Activated potassium channels were found to be strongly correlated with gingival overgrowth (GO) phenotype as we reviewed syndromic hereditary gingival fibromatosis (HGF). Nevertheless, the functional roles of potassium channels in gingival fibrosis or gingival overgrowth remained uncovered. The...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12836

    authors： Gao Q,Yang C,Meng L,Wang Z,Chen D,Peng Y,Yang K,Bian Z

    更新日期：2020-12-31 00:00:00

  • Vital microorganisms in early supragingival dental plaque and in stimulated human saliva.

    abstract：:The aim of this study was to compare the percentage of vital microorganisms (= microbial vitality) of saliva with that of supragingival plaque both collected at various times during the early phases of de novo plaque formation. Between intervals of optimal oral hygiene, 14 healthy participants refrained from all oral ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1997.tb00529.x

    authors： Weiger R,von Ohle C,Decker E,Axmann-Krcmar D,Netuschil L

    更新日期：1997-02-01 00:00:00

  • Relation between periodontal disease and arterial stiffness.

    abstract：BACKGROUND AND OBJECTIVE:Periodontal disease has been described as playing a role in the atherosclerosis process, and its relation with intimal thickness and vascular endothelial function (EF) has been investigated. The present study sought to determine whether there are differences in parameters of arterial stiffness ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12376

    authors： Nicolosi LN,Lewin PG,Rudzinski JJ,Pompeo M,Guanca F,Rodríguez P,Gelpi RJ,Rubio MC

    更新日期：2017-02-01 00:00:00

  • Patients with chronic periodontitis present increased risk for osteoporosis: A population-based cohort study in Taiwan.

    abstract：OBJECTIVES:Chronic periodontitis is a bone destructive inflammatory disease with an adverse impact on general health and suggested underlying factors in common with osteoporosis. A few studies have examined the possible relationship between chronic periodontitis and osteoporosis; however, the results remain inconclusiv...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12464

    authors： Mau LP,Kuan YC,Tsai YC,Lin JJ,Huynh-Ba G,Weng PW,Shieh YS,Cheng WC,Huang RY

    更新日期：2017-10-01 00:00:00

  • Surface characteristics of cells from different layers of keratinized and non-keratinized oral epithelia.

    abstract：:Portions of clinically healthy non-keratinized and keratinized oral epithelia were removed from adult vervet monkeys (Cercopithecus aethiops). The epithelium was separated from the underlying lamina propria by trypsin digestion, following which the epithelial cells were separated from the various epithelial layers. ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1975.tb00011.x

    authors： Cleaton-Jones P

    更新日期：1975-05-01 00:00:00

  • Expression of TIMP-1, TIMP-2 and collagenase mRNA in periodontitis-affected human gingival tissue.

    abstract：:Collagenolysis in periodontitis is thought to be modulated by the expression of three genes, collagenase, tissue inhibitors of metalloproteinases-1 and -2 (TIMP-1 and -2). We assessed the possible difference in TIMP-1, TIMP-2 and collagenase mRNA levels between gingival samples from patients with periodontitis and tho...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.1993.tb01079.x

    authors： Nomura T,Takahashi T,Hara K

    更新日期：1993-09-01 00:00:00

  • Effects of combined use of recombinant human fibroblast growth factor-2 and β-tricalcium phosphate on ridge preservation in dehiscence bone defects after tooth extraction: A split-mouth study in dogs.

    abstract：BACKGROUND AND OBJECTIVE:Following tooth extraction, bone resorption is especially severe in cases complicated with buccal dehiscence bone defects. To minimize this, various bone graft materials have been used for alveolar ridge preservation. This study aimed to evaluate additional effects of the concomitant use of rec...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12818

    authors： Fukuba S,Akizuki T,Matsuura T,Okada M,Nohara K,Hoshi S,Shujaa Addin A,Iwata T,Izumi Y

    更新日期：2020-12-12 00:00:00

  • Human gingival glycosaminoglycans in cyclosporin-induced overgrowth.

    abstract：:Glycosaminoglycans in normal and cyclosporin-induced gingival overgrowth were extracted by papain digestion and purified by Mono Q-FPLC chromatography. The purified glycosaminoglycans were analyzed by agarose gel electrophoresis and by the pattern of degradation products formed by chondroitin lyases on HPLC chromatogr...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1034/j.1600-0765.2000.035003158.x

    authors： Rocha LA,Martins RC,Werneck CC,Feres-Filho EJ,Silva LC

    更新日期：2000-06-01 00:00:00

  • Evaluation of apoptosis and hypoxia-related factors in gingival tissues of smoker and non-smoker periodontitis patients.

    abstract：OBJECTIVE:Smoking causes pathological changes in all tissues, including gingiva and alveolar bone. The aim of present study was to evaluate apoptotic tissue alterations and tissue destruction in smoker and non-smoker periodontitis patients and healthy individuals. METHODS:Gingival biopsy samples from 15 systemically a...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/jre.12723

    authors： Karatas O,Balci Yuce H,Tulu F,Taskan MM,Gevrek F,Toker H

    更新日期：2020-06-01 00:00:00

  • Expression and intracellular localization of progesterone receptors in cultured human gingival fibroblasts.

    abstract：OBJECTIVE:The aim of this immunocytochemical study was to characterize the expression and distribution of the progesterone receptor (PR) and estrogen receptor (ER) in gingival fibroblasts using culture cells derived from people at various ages. BACKGROUND:The reaction of female hormones is tissue or cell specific, and...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1034/j.1600-0765.2003.00654.x

    authors： Kawahara K,Shimazu A

    更新日期：2003-06-01 00:00:00

  • Bursts of periodontal destruction and remission, percolation phase shifts, and chaos.

    abstract：:Bursts of periodontal destruction are modelled as percolation-driven phase shift events. Percolation theory, though widely used to study the flow of liquids through porous nonliving materials (e.g., soils and rocks), can be applied to bacterial or toxicological penetration of living tissue in only an approximate sense...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：

    authors： Cohen ME

    更新日期：1993-11-01 00:00:00

  • Impact of periodontal disease on quality of life: a systematic review.

    abstract：:The diagnosis of periodontal disease is commonly based on objective evaluations of the patient's medical/dental history as well as clinical and radiographic examinations. However, periodontal disease should also be evaluated subjectively through measures that quantify its impact on oral health-related quality of life....

    journal_title：Journal of periodontal research

    pub_type： 杂志文章,评审

    doi：10.1111/jre.12436

    authors： Ferreira MC,Dias-Pereira AC,Branco-de-Almeida LS,Martins CC,Paiva SM

    更新日期：2017-08-01 00:00:00

  • Salivary infectious agents and periodontal disease status.

    abstract：BACKGROUND AND OBJECTIVES:The potential of salivary microorganisms to diagnose periodontal disease and to guide periodontal treatment is a research topic of current interest. This study aimed to determine whether the salivary counts of periodontopathic microbes correlated with the periodontal pocket counts of the same ...

    journal_title：Journal of periodontal research

    pub_type： 杂志文章

    doi：10.1111/j.1600-0765.2010.01335.x

    authors： Saygun I,Nizam N,Keskiner I,Bal V,Kubar A,Açıkel C,Serdar M,Slots J

    更新日期：2011-04-01 00:00:00