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Factors affecting human supragingival biofilm composition. I. Plaque mass.

Abstract：

BACKGROUND AND OBJECTIVE:Little is known about the factors that affect the microbial composition of supragingival biofilms. This study was designed to examine the relationship between total DNA probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition. MATERIAL AND METHODS:Supragingival plaque samples were taken from 187 systemically healthy adult subjects (n = 4745 samples). All samples were individually analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. The relationship between total DNA probe counts and microbial composition was examined by subsetting the data into 10 groups based on 10 percentile increments of the total DNA probe counts. Differences among groups in terms of species counts and proportions were sought, as well as relationships of total plaque DNA probe count and clinical parameters. RESULTS:There was a wide distribution in mean total DNA probe counts among the 187 subjects. With increasing total plaque levels there was a change in the proportions of individual species and microbial complexes. 'Small plaques' were characterized by high proportions of species in the yellow, orange, purple and 'other' complexes; plaques of moderate mass were characterized by high proportions of Actinomyces and purple complex species, while 'large plaques' exhibited increased proportions of green and orange complex species. Measures of gingival inflammation, pocket depth and recession were significantly positively associated with total DNA probe counts. Increased plaque numbers were related to increased pocket depth irrespective of presence or absence of gingival inflammation. CONCLUSION:The proportions of individual species and microbial complexes in supragingival biofilms are influenced by the total numbers of organisms in the biofilm.

journal_name

J Periodontal Res

journal_title

Journal of periodontal research

authors

Haffajee AD,Teles RP,Patel MR,Song X,Veiga N,Socransky SS

doi

10.1111/j.1600-0765.2008.01154.x

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

511-9

issue

eissn

0022-3484

issn

1600-0765

pii

JRE1154

journal_volume

pub_type

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Factors affecting human supragingival biofilm composition. I. Plaque mass.