Abstract:
:The degradation of exogenous radioactively labeled fatty acids by 5-day-old barley shoots was examined. [1-(14)C] Linoleic acid was observed to be degraded 7 times faster than [1-(14)C] oleic acid and 5 times faster than [1-(14)C] palmitic acid. The pathway of degradation was determined by identifying the water-soluble products and determined to be β-oxidation. During a 15 min incubation, the barley shoots took up 0.91 nmol/g fresh wt of linoleic acid, of which 0.16 nmol/g fresh wt was incorporated into glutamic acid, 0.07 nmol/g fresh wt into succinic acid and 0.002 nmol/g fresh wt into carbohydrates. By 30 min, additional TCA cycle intermediates, especially malic acid, were detected. Palmitic acid and oleic acid were broken down to the same products. The rates of uptake and the distribution of label into lipids were determined. The uptake of label by the tissue was similar for all 3 fatty acid substrates. Label from linoleic, oleic and palmitic acids was found to be incorporated into similar lipids, primarily phosphatidylcholine (PC), and the extent of incorporation was comparable. Although all 3 fatty acid substrates were broken down by β-oxidation, the reason for the more rapid degradation of linoleic acid was not established. It does not appear to be related to uptake of substrate or incorporation of label into lipids.
journal_name
Lipidsjournal_title
Lipidsauthors
Nabors LA,Morgan MS,Newman DW,Jaworski JGdoi
10.1007/BF02534483subject
Has Abstractpub_date
1984-07-01 00:00:00pages
507-14issue
7eissn
0024-4201issn
1558-9307journal_volume
19pub_type
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