Abstract:
:By using reverse transcription-polymerase chain reaction technology (RT-PCR) and Northern blot analysis, the tissue-specific mRNA expression patterns of seven mouse lipoxygenases (LOX)--including 5S-, 8S-, three isoforms of 12S-, 12R-LOX, and a LOX of an as-of-yet unknown specificity, epidermis-type LOX-3 (e-LOX-3)--were investigated in NMRI mice. Among the various tissues tested epidermis and forestomach were found to express the broadest spectrum of LOX. With the exception of 5S- and platelet-type 12S-LOX (p12S-LOX) the remaining LOX showed a preference to exclusive expression in stratifying epithelia of the mouse, in particular the integumental epidermis. The expression of the individual LOX in mouse epidermis was found to depend on the state of terminal differentiation of the keratinocytes. mRNA of epidermis-type 12S-LOX (e12S-LOX) was detected in all layers of neonatal and adult NMRI mouse skin, whereas expression of p12S-LOX, 12R-LOX, and e-LOX-3 was restricted to suprabasal epidermal layers of neonatal and adult mice. 8S-LOX mRNA showed a body-site-dependent expression in that it was detected in stratifying epithelia of footsole and forestomach but not in back skin epidermis. In the latter, 8S-LOX mRNA was strongly induced upon treatment with phorbol esters. With the exception of e12S-LOX and p12S-LOX, the isozymes that are preferentially expressed in stratifying epithelia are structurally related and may be grouped together into a distinct subgroup of epidermis-type LOX.
journal_name
Lipidsjournal_title
Lipidsauthors
Heidt M,Fürstenberger G,Vogel S,Marks F,Krieg Pdoi
10.1007/s11745-000-0576-0subject
Has Abstractpub_date
2000-07-01 00:00:00pages
701-7issue
7eissn
0024-4201issn
1558-9307journal_volume
35pub_type
杂志文章相关文献
LIPIDS文献大全abstract::The catfish, Ictalurus punctatus, is an important model for studying the biochemical mechanisms of taste at the peripheral level. The type, amount and metabolic activity of the lipids within this tissue play important roles in taste transduction by forming the matrix in which the receptors for taste stimuli are imbedd...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535745
更新日期:1990-04-01 00:00:00
abstract::A protocol using a dry column method was modified for the extraction of total lipids and the simultaneous separation and quantitation of neutral and polar lipids in human milk. The triacylglycerol, cholesterol, phospholipid and vitamin E contents of the lipid extracts were determined and compared with lipids extracted...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535216
更新日期:1989-08-01 00:00:00
abstract::Mycobacterium fortuitum utilizes certain stereoisometric mixtures of individual multimethyl branched alkanes as sole carbon source, including 2,6(R), 10(S), 14(RS)-tetramethylhexadecane; 2.6(R), 10(S), 14(RS)-tetramethylheptadecane; 2,6(RS), 10(RS)-trimethyltetradecane, and 2,6(R), 10(S)-trimethylpentadecane. Products...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532587
更新日期:1976-01-01 00:00:00
abstract::The effects of increasing concentrations of eicosapentaenoic acid (20:5n-3; EPA) and oleic acid (18:1n-9; OA) on esterification to triacylglycerols (TG) and phospholipids (PL), and the relationship to formation and secretion of the very low density lipoproteins (VLDL) were compared in the isolated perfused rat liver. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535940
更新日期:1993-05-01 00:00:00
abstract::The beneficial roles of dietary fish oil in lowering serum TAG levels in animals and humans have been attributed in part to the high content of two n-3 polyunsaturated very long-chain FA, EPA, and DHA. Recent studies show that EPA induces mitochondrial beta-oxidation in hepatocytes, which might contribute to the syste...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-005-1443-8
更新日期:2005-08-01 00:00:00
abstract::The aim of this study was to develop a simple method for simultaneous determination of selected cis/cis PUFA-LNA (18:2), ALA (18:3), GLA (18:3), EPA (20:5), and DHA (22:6) by silver ion high-performance liquid chromatography coupled to a diode array detector (Ag-HPLC-DAD). The separation was performed on three Luna SC...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-016-4133-1
更新日期:2016-04-01 00:00:00
abstract::The effects of different dietary fats on ovine adipose tissue metabolism have been investigated. Six-month old sheep were fed for 6 weeks a control diet or diets supplemented with either tallow or a mixture of sunflower seed oil and soybean oil, treated to protect the fats from hydrolysis and hydrogenation in the rume...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02532883
更新日期:1976-09-01 00:00:00
abstract::Observational evidence suggests that in populations consuming low levels of n-3 highly unsaturated fatty acids, women have higher blood levels of docosahexaenoic acid (DHA; 22:3n-6) as compared with men. Increased conversion of alpha-linolenic acid (ALA; 18:3n-3) to DHA by females has been confirmed in fatty acid stab...
journal_title:Lipids
pub_type: 杂志文章,评审
doi:10.1007/s11745-010-3391-6
更新日期:2010-03-01 00:00:00
abstract::The tissue distribution of newly synthesized 22:6n-3 and intermediate PUFA was examined in rainbow trout to further our understanding of the metabolism of this EFA in fish. Rainbow trout were fed a pulse of deuterated linolenic acid (D5-17,17,18,18,18-18:3n-3), and the tissue distribution of deuterated anabolites was ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-003-1146-1
更新日期:2003-09-01 00:00:00
abstract::We have previously reported that the relative content of 18:3 omega 3 in adipose triglyceride (TG) of women was reduced following major weight loss while on a very low calorie diet (VLCD). In an attempt to prevent this loss of 18:3 omega 3 reserves, we have tested two VLCD supplemented with varying amounts of 18:3 ome...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537119
更新日期:1993-11-01 00:00:00
abstract::The particular interest in supplementing human foods with n-3 fatty acids has arisen from the findings that this series of polyunsaturated fatty acids (PUFA) have an impact on neuronal functions. Indeed vertebrates, including humans, preferentially use docosahexaenoic acid (DHA, 22:6n-3) over other long-chain n-3 PUFA...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-012-3686-x
更新日期:2012-08-01 00:00:00
abstract::Two simple methods for the synthesis of phosphatidylcholine (lecithin) isotopically labeled in the methyl group of the choline moiety are described. Phosphatidylcholine is converted to phosphatidyl-ethanolamine by enzymic transphosphatidylation, and this is methylated using either methyl iodide or diazomethane to give...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533373
更新日期:1978-01-01 00:00:00
abstract::Two unusual lipid classes were detected by thin-layer chromatography in the neutral lipids derived from goat cauda-epididymal sperm plasma membrane. The lipids were identified as wax esters and 1-O-alkyl-2,3-diacylglycerols based on chromatographic properties, identity of their hydrolysis products, and infrared/1H nuc...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02537065
更新日期:1992-01-01 00:00:00
abstract::Lipids were extracted from four dog adipose tissue sites, perirenal, pericardial, inguinal subcutaneous, and mesenteric. The triglycerides were purified by thin-layer chromatography and the sub-classes separated by silver nitrate-silica gel plates. Gas chromatography was used to delineate the fatty acid composition of...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02530899
更新日期:1968-11-01 00:00:00
abstract::The farnesoid X receptor (FXR, NR1H4) has been recognized as an attractive therapeutic target because it is a nuclear hormone receptor that controls the expression level of cholesterol-7alpha-hydroxylase, which in turn regulates bile acid production and cholesterol excretion. To compare receptor activity between each ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-003-1173-y
更新日期:2003-11-01 00:00:00
abstract::The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7 +/- 0.2% (2...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-000-0619-6
更新日期:2000-10-01 00:00:00
abstract::Because bile contains substantial amounts of cholesterol precursors, e.g., squalene and different methyl sterols, the fate of biliary squalene was studied by incubating isolated jejunal loops of the rat in vivo with bile containing 3H-squalene and 14C-cholesterol. After 90 min, no radioactivity was found in plasma lip...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535392
更新日期:1983-08-01 00:00:00
abstract::The effects of 2-hexadecynoic acid on the growth and lipid metabolism of cultured 7288 (HTC) cells have been evaluated. Growth was inhibited by the acetylenic acid: the LD50 was 35-85 microM as determined by two methods at low and high cell densities. Reduced growth did not result from damaged plasma membranes as dete...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534955
更新日期:1981-05-01 00:00:00
abstract::To test the effects of eicosapentaenoic acid (EPA) infusion on pulmonary edema induced by coronary ligation and reperfusion, extravascular lung water (EVLW) was measured in situ by the thermal-dye double indicator dilution method in dogs. In the control group of five dogs, 30 mL of a 10% soybean oil emulsion was infus...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-997-0015-2
更新日期:1997-01-01 00:00:00
abstract::The uptake of lipid from the yolk by the yolk sac membrane of the chick embryo is accompanied by the rapid esterification of a large proportion of the yolk cholesterol. This could arise from enhanced acyl-CoA:cholesterol acyltransferase (ACAT) activity and/or inhibition of cholesteryl ester hydrolase (CEH) activity. T...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536056
更新日期:1993-07-01 00:00:00
abstract::Cholesterol absorption is frequently determined using the plasma dual stable-isotope ratio method (PDSIRM). However, this method involves intravenous injection of stable-isotope-labeled cholesterol with simultaneous oral administration of differently labeled cholesterol, which results in high study costs and involves ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-004-1206-6
更新日期:2004-01-01 00:00:00
abstract::Earlier studies showed that during the first 20 to 25 seconds of aggregation induced by thrombin (0.1 U/mL) or adenosine diphosphate (ADP) (2 microM) of rabbit or human platelets prelabeled with [3H]palmitic acid, labeled lipid became associated with the cytoskeleton (isolated after lysis with 1% Triton X-100, 5 mM EG...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02536590
更新日期:1991-07-01 00:00:00
abstract::Plaques and white matter from brains of multiple sclerosis (MS) patients were analyzed for lipid content, class composition, and fatty acid composition of total lipid, together with the fatty acid composition of plaque glycerophospholipids, and the results were compared with white matter from normal brain. Plaques con...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02544017
更新日期:1991-01-01 00:00:00
abstract::In animals, non-sterol metabolites of the mevalonate pathway act independently from receptor-mediated cholesterol uptake in the multivalent feedback regulation of mevalonate biosynthesis. Studies leading to the isolation and characterization of plant-borne suppressors of mevalonate biosynthesis are reviewed. We propos...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02534407
更新日期:1985-11-01 00:00:00
abstract::This study evaluated the effects of conjugated linoleic acids (CLA) on tissue fatty acid composition and ex vivo prostaglandin E2 (PGE2) production in rats given diets varying in n-6 and n-3 fatty acids. Four groups of rats were given a basal semipurified diet (AIN-93G) containing 70 g/kg of added fat for 42 d. The fa...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-998-0223-9
更新日期:1998-04-01 00:00:00
abstract::The stereochemistry of fat digestion and absorption was investigated in rats with thoracic duct fistulas, after feeding synthetic triacylglycerol or alkyldiacylglycerol. After feeding 1,2-dilauroyl-3-oleoyl-sn-glycerol, dilauroyloleoylglycerol and lauroyldioleoylglycerol were the most abundant chyle triacylglycerols. ...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02533725
更新日期:1978-05-01 00:00:00
abstract::We recently reported the presence of various cyclopropane fatty acids-among them, cyclopropaneoctanoic acid 2-hexyl-in the adipose tissue of obese women. The aim of this study was to verify whether the presence of cyclopropaneoctanoic acid 2-hexyl in human serum was associated with obesity or chronic kidney disease (b...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/s11745-016-4141-1
更新日期:2016-07-01 00:00:00
abstract::31P Nuclear magnetic resonance (NMR) spectra of human erythrocyte lysates dissolved in sodium cholate were acquired. The narrow resonances of phospholipids were mostly well resolved, allowing identification and accurate quantitative analysis of phospholipid classes of the erythrocyte membranes. The ether-linked phosph...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02522893
更新日期:1996-07-01 00:00:00
abstract::Bile acid contents were determined for isolated rat hepatocytes. During the course of isolating the hepatocytes, perfusion of rat liver with buffer containing 2% albumin eluted a significant amount of bile acids. The elution was proportional to the volume of the buffer and attributable to albumin in the buffer. The is...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02535094
更新日期:1982-03-01 00:00:00
abstract::The effect of hydroperoxy and hydroxy derivatives of various fatty acids on human platelet aggregation was determined to delineate potencies and structure-activity function. In this regard, the 22-carbon n-3 fatty acids are the most potent inhibitors in comparison to the n-6 lipoxygenase derivatives. Submicromolar lev...
journal_title:Lipids
pub_type: 杂志文章
doi:10.1007/BF02637097
更新日期:1996-03-01 00:00:00