Abstract：
:Success in synthetic biology and metabolic engineering is quickly becoming 'test' limited within the design-build-test cycle. Commonly used methods for high-throughput screening, including fluorescence-activated cell sorting (FACS) and microtiter plates, have intracellular product and throughput limitations. A growing alternative to these challenges is the use of microfluidic microdroplet-based methods, which offer the advantages of microtiter plates with the throughput and ease of flow-based approaches. In this review, we examine available microdroplet technologies and their applications from droplet generation to sensing and finally sorting and evaluation for metabolic engineering applications. Additionally, we cover recent microdroplet advances, including the ability to perform mass spectrometry (MS) on individual microdroplets and dispense them into microtiter plates after sorting via fluorescence-activated droplet sorting (FADS).
journal_name
Trends Biotechnoljournal_title
Trends in biotechnologyauthors
Bowman EK,Alper HSdoi
10.1016/j.tibtech.2019.11.002subject
Has Abstractpub_date
2020-07-01 00:00:00pages
701-714issue
7eissn
0167-7799issn
1879-3096pii
S0167-7799(19)30269-0journal_volume
38pub_type
杂志文章,评审abstract：:Type 1 Diabetes (T1D) is an autoimmune disease resulting from the destruction of pancreatic insulin-producing beta cells that most frequently occurs in genetically predisposed children. Recent observations illustrating the regenerative capability of the endocrine pancreas in addition to advances in stem cell and gene ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2006.08.007
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abstract：:Nanotechnology is currently driving the dental materials industry to substantial growth, thus reflecting on improvements in materials available for oral prevention and treatment. The present review discusses new developments in nanotechnology applied to dentistry, focusing on the use of nanomaterials for improving the...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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abstract：:The current productivity crisis in drug discovery has prompted the pharmaceutical industry to decentralize R&D, which is now more responsive, more flexible and better connected to research in academia and biotechnology firms. Organizational changes are also under way in academia. Universities are expanding their techn...
journal_title：Trends in biotechnology
pub_type： 杂志文章
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abstract：:Hydrophobic charge induction chromatography using 4-mercapto-ethyl-pyridine as the ligand is an effective method for the separation of antibodies from a variety of feedstocks. Antibodies are adsorbed in physiological conditions without preliminary concentration. Desorption occurs when the pH is lowered, thus inducing ...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0167-7799(02)01980-7
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abstract：:Rice is the staple diet of more than three billion people. Yields must double over the next 40 years if we are to sustain the nutritional needs of the ever-expanding global population. Between 10% and 30% of the annual rice harvest is lost due to infection by the rice blast fungus Magnaporthe oryzae. Evaluation of gen...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2008.12.002
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abstract：:Unbalanced production of atmospheric CO(2) constitutes a major challenge to global sustainability. Technologies have thus been developed for enhanced biological carbon fixation (also referred to as CO(2) mitigation), and one of the most promising capitalizes on microalgae. However, the "best bioreactor", which would b...
journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2020.04.006
更新日期：2020-12-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2016.06.003
更新日期：2016-12-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/0167-7799(93)90156-4
更新日期：1993-08-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2013.01.015
更新日期：2013-06-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2012.07.003
更新日期：2012-11-01 00:00:00
abstract：:Bioelectrochemical power-to-gas (BEP2G) is considered a potentially convenient way of storing renewable surplus electricity in the form of methane. In methane-producing bioelectrochemical systems (BESs), carbon dioxide and electrical energy are converted into methane, using electrodes that supply either electrons or h...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2016.08.010
更新日期：2016-11-01 00:00:00
abstract：:Microbial consortia have been used in biotechnology processes, including fermentation, waste treatment, and agriculture, for millennia. Today, synthetic biologists are increasingly engineering microbial consortia for diverse applications, including the bioproduction of medicines, biofuels, and biomaterials from inexpe...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.11.002
更新日期：2019-02-01 00:00:00
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journal_title：Trends in biotechnology
pub_type： 杂志文章
doi：10.1016/j.tibtech.2009.09.002
更新日期：2009-12-01 00:00:00
abstract：:Vascularisation is key to developing large transplantable tissue constructs capable of providing therapeutic benefits. The vascularised tissue engineering chamber originates from surgical concepts in tissue prefabrication and microsurgery. It serves as an in vivo bioreactor in the form of a closed, protected space sur...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2018.05.009
更新日期：2018-10-01 00:00:00
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journal_title：Trends in biotechnology
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journal_title：Trends in biotechnology
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doi：10.1016/j.tibtech.2010.09.004
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abstract：:Protein engineers traditionally rely on amino acid substitutions to alter the functional properties of biomacromolecules, yet have largely overlooked the potential benefits of reorganizing the polypeptide chain of a protein by circular permutation (CP). By connecting the native protein termini via a covalent linker an...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.10.004
更新日期：2011-01-01 00:00:00
abstract：:Microalgal production technologies are seen as increasingly attractive for bioenergy production to improve fuel security and reduce CO(2) emissions. Photosynthetically derived fuels are a renewable, potentially carbon-neutral and scalable alternative reserve. Microalgae have particular promise because they can be prod...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2011.11.003
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journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
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journal_title：Trends in biotechnology
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abstract：:Proteins found in nature have traditionally been the most frequently used biocatalysts to produce numerous natural products ranging from commodity chemicals to pharmaceuticals. Protein engineering has emerged as a powerful biotechnological toolbox in the development of metabolic engineering, particularly for the biosy...
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2019.12.008
更新日期：2020-07-01 00:00:00
abstract：:The ability to detect single-base changes is of fundamental importance in molecular genetics. This is particularly true in human genetics, where interest in linking mutations of identified genes to particular disease phenotypes is most intense, and where a demand exists for clinical diagnosis of defined mutations. In ...
journal_title：Trends in biotechnology
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abstract：:Microbial contamination of polymer surfaces has become a significant challenge in domestic, industrial, and biomedical applications. Recent progress in our understanding of how topographical features of different length scales can be used to effectively and selectively control the attachment and proliferation of diffe...
journal_title：Trends in biotechnology
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更新日期：2020-05-01 00:00:00
abstract：:Biohacking is a do-it-yourself citizen science merging body modification with technology. The motivations of biohackers include cybernetic exploration, personal data acquisition, and advocating for privacy rights and open-source medicine. The emergence of a biohacking community has influenced discussions of cultural v...
journal_title：Trends in biotechnology
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更新日期：2018-08-01 00:00:00
abstract：:Anywhere water is in the liquid state, bacteria will exist as biofilms, which are complex communities of cells that are cemented together. Although frequently associated with disease and biofouling, biofilms are also important for engineering applications, such as bioremediation, biocatalysis and microbial fuel cells....
journal_title：Trends in biotechnology
pub_type： 杂志文章,评审
doi：10.1016/j.tibtech.2010.11.001
更新日期：2011-02-01 00:00:00
