Automated determination of neutrophil VCS parameters in diagnosis and treatment efficacy of neonatal sepsis.

Abstract:

INTRODUCTION:The Coulter LH780 hematology analyzer can evaluate mean neutrophil volume (MNV), conductivity (MNC), scatter (MNS), and distribution width (DW). We sought to investigate the value of volume, conductivity, and scatter (VCS) parameters in diagnosis and treatment efficacy of neonatal sepsis. RESULTS:We observed significant increases in MNV, volume distribution width (VDW), conductivity distribution width (CDW), and significant decreases in MNC and MNS in septic newborns. There were significant decreases in MNV, VDW, and CDW, whereas MNC and MNS increased at the end of the treatment. Gram-negative sepsis caused higher MNV and VDW than Gram-positive sepsis. DISCUSSION:This is the largest reported study seeking to determine cutoff levels of neutrophil VCS parameters in diagnosis of sepsis, and the first study in the evaluation of treatment efficacy and the effects of sepsis onset time and birth weight. We suggest that neutrophil VCS parameters and their DWs are useful both for early diagnosis and evaluation of treatment efficacy in neonatal sepsis without requirement for any extra blood collection. METHODS:Peripheral blood samples from 304 newborns, 206 in group I (76 proven and 130 clinical sepsis) and 98 in group II (control group), were studied on diagnosis, 3rd day, and at the end of the treatment.

journal_name

Pediatr Res

journal_title

Pediatric research

authors

Celik IH,Demirel G,Aksoy HT,Erdeve O,Tuncer E,Biyikli Z,Dilmen U

doi

10.1038/pr.2011.16

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

121-5

issue

1

eissn

0031-3998

issn

1530-0447

pii

pr201116

journal_volume

71

pub_type

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