A paper chromatoelectrophoresis method for the determination of bilirubin binding to serum protein.

Abstract:

:A technique is described in which paper chromatoelectrophoresis is used to measure the bilirubin binding capacity of albumin in serum. It is equivalent to the Sephadex G-25 method and its results are highly reproducible. From a single pool of serum, multiple determinations indicated the mean bilirubin binding capacity to be 23.7 +/- 0.76 (SD) mg/dl and the greatest difference between any two values was 2 mg/dl. This new technique can determine the bilirubin binding capacity of a serum sample in 15 min. Highly skilled technicians are not needed and very small quantities of serum are required, which gives it a definite advantage over the Sephadex G-25 method.

journal_name

Pediatr Res

journal_title

Pediatric research

authors

Ainbender E,Brown EG,Lee YJ,Sweet AY

doi

10.1203/00006450-197707000-00006

subject

Has Abstract

pub_date

1977-07-01 00:00:00

pages

808-11

issue

7

eissn

0031-3998

issn

1530-0447

journal_volume

11

pub_type

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