Abstract:
BACKGROUND:We previously demonstrated that the proliferative response to lipopolysaccharide (LPS) in cord blood mononuclear cells (CBMCs) is negatively correlated with the induced expression of interleukin (IL)-4. Our aim, therefore, was to examine whether an impaired cellular response to LPS in infancy is associated with the risk for asthma. METHODS:In a prospective cohort study, the relationship between the CBMC response to LPS and the risk of atopy and wheezing after the age of 4 y was evaluated. RESULTS:LPS-induced CBMC proliferative responses varied markedly among the 102 infants studied (range, one- to fivefold increase over cells with diluent alone). Ninety-five infants (93%) were followed longitudinally. A higher CBMC proliferative response to LPS was noted in offspring born to nonatopic parents compared with those with at least one atopic parent (P = 0.008). Using a proliferative index cutoff of 2 separated patients into high and low induced IL-4 mRNA responders (P = 0.001). Significantly more children who never wheezed had a greater than twofold LPS-induced CBMC proliferative response compared to those with persistent atopic wheezing (P = 0.046). CONCLUSION:These results demonstrate that CBMC proliferative responses to LPS is impaired in infants born to atopic parents and may be a risk factor for asthma later in life.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Goldberg MR,Elizur A,Luknar-Gabor N,Koch P,Tovbin J,Katz Ydoi
10.1038/pr.2013.74subject
Has Abstractpub_date
2013-08-01 00:00:00pages
211-6issue
2eissn
0031-3998issn
1530-0447pii
pr201374journal_volume
74pub_type
杂志文章abstract::Phagocytosis of apoptotic cells, e.g., neutrophils, by monocytes is essential for resolution of inflammation. Delayed removal leads to secondary necrosis, perpetuating inflammation, and tissue destruction. Common histologic features in neonatal chronic inflammatory disorders are an accumulation of apoptotic cells in i...
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journal_title:Pediatric research
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1996-09-01 00:00:00
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journal_title:Pediatric research
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更新日期:2011-05-01 00:00:00
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pub_type: 杂志文章,多中心研究
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abstract::Apoptosis of CD4+ and CD8+ T cells has been shown in peripheral blood mononuclear cells (PBMCs) from HIV-infected adults analyzed after overnight culture. Because cell death may be an artifact of in vitro culture, and because there is little information on apoptosis in pediatric HIV disease, we undertook a cross-secti...
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abstract::The pathophysiology of kwashiorkor, a severe edematous manifestation of malnutrition, is still poorly understood. The syndrome is, however, known to be associated with alterations in redox metabolism. To further elucidate the role of oxidative stress in kwashiorkor, we carried out a longitudinal study on the major blo...
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abstract::Human and ovine placental tissue homogenates were assayed for dipeptidase activity in vitro. Glycyl-L-leucine, L-leucyl glycine, glycyl-L-lysine, and L-lysyl glycine were hydrolyzed by placental homogenates. The pH optimum for the reaction was 8.0. The relationship between enzyme activity and concentration was linear ...
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doi:10.1203/00006450-199910000-00018
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章
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