Abstract:
:Up to 20% of pregnant women smoke and there is indirect evidence that certain tobacco-specific metabolites can cross the placental barrier and are genotoxic to the fetus. The presence of micronuclei results from chromosome damage and reflects the degree of underlying genetic instability. Fetal blood was obtained from the cord blood of 143 newborns (102 from nonsmoking mothers and 41 from mothers smoking >10 cigarettes/d during pregnancy). The micronucleus assay was performed following the guidelines established by the Human MicroNucleus project with modifications. To test the micronucleus assay, we evaluated the effect of a range of benzopyrene-7,8-diol-9,10-epoxide concentrations (from 3.125 nM to 4 microM) on cord blood from nonsmoking mothers. This validation showed that the number of micronuclei and apoptotic cells increased with benzopyrene-7,8-diol-9,10-epoxide dose (p < 0.0001 and p = 0.001, respectively); the minimal detectable effect was induced by 12.5 nM benzopyrene-7,8-diol-9,10-epoxide. In our sample, the number of MN was significantly higher in the 41 cord blood samples from mothers who smoked during pregnancy [smokers: 4 (1; 10.5); nonsmokers: 3 (0; 8); p = 0.016]. Therefore, the data reported herein support the hypothesis that tobacco compounds are able to induce chromosomal losses and breaks that are detectable as an increased number of micronuclei.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Zalacain M,Sierrasesumaga L,Larrañnaga C,Patiñno-García Adoi
10.1203/01.pdr.0000228327.63820.41subject
Has Abstractpub_date
2006-08-01 00:00:00pages
180-4issue
2eissn
0031-3998issn
1530-0447pii
60/2/180journal_volume
60pub_type
杂志文章abstract::Twelve-h overnight urine and serum samples obtained simultaneously at 20-min intervals were assayed for growth hormone (GH). Ninety-one children, 5 to 16 y (Tanner stage 1 to 3) participated; group 1 were healthy children, group 2 were children with organic GH deficiency, and group 3 had idiopathic growth failure and ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199106010-00019
更新日期:1991-06-01 00:00:00
abstract::The IQ's of Jamaican boys aged 6-10 were associated significantly with the presence or absence of severe malnutrition in infancy, with height at time of IQ testing, and with a measure of the boys' social background. A multiple correlation coefficient of 0.674 was obtained between IQ and the three factors. Social backg...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197601000-00011
更新日期:1976-01-01 00:00:00
abstract::The interrelationship of 2,3-diphosphoglycerate (2,3-DPG) and P50 levels during the fetal and postnatal life were determined in two mammalian species which do not have a switchover of hemoglobin type at the end of their fetal development. In the guinea pig and rabbit, the 2,3-DPG levels remain low during fetal life an...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197903000-00006
更新日期:1979-03-01 00:00:00
abstract::The objective of this study was to obtain insight into bone formation and resorption in children with newly diagnosed untreated acute lymphoblastic leukemia (ALL). In 23 consecutive children with ALL, a bone biopsy was taken from the crista iliaca posterior under ketamine anesthesia, together with the diagnostic marro...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000090929.16450.DE
更新日期:2003-12-01 00:00:00
abstract::High-frequency oscillatory ventilation (HFOV) may improve pulmonary outcome in very preterm infants, but the effects on the brain are largely unknown. We hypothesized that early prolonged HFOV compared with low volume positive pressure ventilation (LV-PPV) would not increase the risk of delayed brain growth or injury ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181bb0cc1
更新日期:2009-11-01 00:00:00
abstract::In spontaneously breathing newborn lambs, alveolar hypoxia increases lung microvascular pressure, which causes lung lymph flow to increase and the concentration of protein in lymph to decrease. To see if this response derives from hypoxia itself rather than from the change in breathing pattern that occurs during hypox...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198405000-00009
更新日期:1984-05-01 00:00:00
abstract::Cardiovascular responses to feeding have been observed in several species during various periods of development and have been implicated in the development of cardiovascular regulation. In the rat, these responses are characterized by short-lasting, large increases in blood pressure (BP) and moderate increases in hear...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199207000-00007
更新日期:1992-07-01 00:00:00
abstract::Studies were conducted to assess the efficacy and safety of a synthetic peptide-containing surfactant in the treatment of respiratory distress syndrome (RDS) in preterm (approximately 80% of normal gestation) infant rhesus monkeys. Surfactant was prepared consisting of the phospholipids dipalmitoylphosphatidyl choline...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199604000-00025
更新日期:1996-04-01 00:00:00
abstract::Essential fatty acid deficiency (EFAD) occurs in infants fed fat-free mixtures of glucose and amino acids. Although infusion of lipid emulsion rapidly reverses clinical symptoms, little is known about effects on tissue fatty acids. To study this question, five groups (n = 4/group) of neonatal pigs were studied. Three ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198412000-00027
更新日期:1984-12-01 00:00:00
abstract::Mucoid Pseudomonas aeruginosa is an important respiratory pathogen in patients with cystic fibrosis, and once acquired is virtually impossible to eradicate. Although mucoid P. aeruginosa is generally believed to be resistant to phagocytosis, the mechanism is not understood fully. We studied the nonopsonic phagocytosis...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198710000-00013
更新日期:1987-10-01 00:00:00
abstract::Diarrheal disease is a worldwide problem that still causes significant morbidity and mortality among children. Currently, oral rehydration solution (ORS) is the standard of care for acute diarrhea in pediatric patients. Although effective in reducing mortality, ORS does not alleviate diarrheal symptoms, thus reducing ...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/pr.2017.295
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Staphylococcus aureus is the leading cause of skin and soft tissue infections (SSTIs). To develop interventions to prevent recurrent infections, household attributes and individual practices influencing S. aureus colonization must be discerned. METHODS:Households of healthy children with methicillin-resista...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-018-0113-x
更新日期:2018-11-01 00:00:00
abstract::BackgroundAcute kidney injury (AKI) is common in pediatric inpatients and is associated with increased morbidity, mortality, and length of stay. Its early identification can reduce severity.MethodsTo create and validate an electronic health record (EHR)-based AKI screening tool, we generated temporally distinct develo...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2017.116
更新日期:2017-09-01 00:00:00
abstract::An Epstein-Barr virus-transformed B-cell line derived from a patient with severe combined immunodeficiency who died of a lymphoreticular malignancy has been characterized. The line derived from bone marrow cultures and designated DV-1 shows surface and cytoplasmic IgM and staining with fluorescent monoclonal antibodie...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198704000-00003
更新日期:1987-04-01 00:00:00
abstract::The intranasal live attenuated influenza vaccine (LAIV), FluMist, has been widely appreciated by pediatricians, parents, and children alike for its ease of administration. However, concerns regarding lack of effectiveness in recent influenza seasons led to the CDC Advisory Committee on Immunization Practices (ACIP) re...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/pr.2017.239
更新日期:2018-01-01 00:00:00
abstract::A series of in vivo experiments is described in which [1-13C]phytanic acid was given as an oral substrate to a healthy subject and two patients showing an impairment in phytanic acid degradation, one with Refsum's disease and one with chondrodysplasia punctata. After intake of the substrate by the control in a dose of...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199211000-00016
更新日期:1992-11-01 00:00:00
abstract::A child presented with obstructive sleep apnea (OSA) and a near-fatal airway obstruction and respiratory arrest shortly after receiving chloral hydrate (CH). We, therefore, hypothesize that CH might selectively depress upper airway maintaining muscles such as the genioglossus and so predispose to airway obstruction. G...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198406000-00006
更新日期:1984-06-01 00:00:00
abstract::N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) and N-formyl-L-methionyl-L-phenylalanine (FMP) were used to investigate neutrophil (PMN) aggregation. Neutrophils were isolated from healthy adult volunteers and term newborn cord blood. Neutrophil aggregation was measured after the addition of FMLP and FMP. Adult P...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198312000-00013
更新日期:1983-12-01 00:00:00
abstract::Girls with Turner syndrome (TS) are treated with supraphysiological doses of growth hormone (GH) to improve final height; however in some girls, the growth response can be poor. This may reflect aberrations in GH and/or IGF-I actions at the cellular level, and thus this study compared the response of skin fibroblasts ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31821b570b
更新日期:2011-07-01 00:00:00
abstract::Inhibition of IGF-I action by circulating IGF-I receptor autoantibodies is a potential mechanism of IGF-I resistance in growing children. To define the prevalence of IGF-I receptor antibodies in short-statured children, we have examined serum and plasma samples from a well-characterized group of 34 short, prepubertal,...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199210000-00016
更新日期:1992-10-01 00:00:00
abstract::Birth is characterized by swift and complex transitions in hemodynamic and respiratory variables. Unrecognized pathologies or incidents may quickly become fatal or cause permanent damage. This article introduces an essential component of an acute perinatal care simulator, namely a model for educational simulation of n...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181c2def3
更新日期:2010-02-01 00:00:00
abstract:BACKGROUND:Neonatal seizures can result in chronic epilepsy and long-term behavioral and cognitive deficits. Levetiracetam (LEV), an antiepileptic drug that binds to the synaptic vesicle protein 2A (SV2A), has been increasingly used off-label for the therapy of neonatal seizures. Preclinical data regarding the acute or...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.151
更新日期:2013-01-01 00:00:00
abstract::We evaluated bone mineralization by single photon absorptiometry at 2 y in a cohort of preterm infants studied since birth. Infants were fed human milk fortified with Ca [to achieve 80 mg/dL (19.96 mmol/L)] and P [40 mg/dL (12.91 mmol/L)] from wk 2 through 8 after birth. After hospital discharge, infants were divided ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199206000-00009
更新日期:1992-06-01 00:00:00
abstract::Accurate interpretation of the results of GH stimulation tests is of pivotal importance not only in the evaluation of the etiology of growth retardation in children but also in the selection of the best candidates for GH therapy. We performed this study to test a novel immunofunctional GH ( IFGH) assay that makes use ...
journal_title:Pediatric research
pub_type: 评论,杂志文章
doi:10.1203/00006450-200011000-00010
更新日期:2000-11-01 00:00:00
abstract::Murine hybridoma antibodies directed against the capsule and O-side chain determinants of the Escherichia coli strain Bort (018ac:K1:H7) were evaluated for their ability to enhance bactericidal activity of cord blood against K1 E. coli strains possessing O antigens common in neonatal E. coli infection, i.e. 018, 07, a...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199012000-00025
更新日期:1990-12-01 00:00:00
abstract:BACKGROUND:Infantile hemangioma (IH) is the most frequent vascular tumor of early childhood. Recently, propranolol, a nonselective β1- and β2-adrenoceptor inhibitor, was introduced into the therapy of severe proliferating IH with excellent results. However, the underlying mechanism of action of propranolol is still unc...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2013.16
更新日期:2013-04-01 00:00:00
abstract::Prolonged unconjugated hyperbilirubinemia in infants associated with breast milk feeding is a common pediatric problem known as breast milk jaundice (BMJ). A polymorphic mutation (G71R) of bilirubin UDP-glucuronosyltransferase (UGT1A1) is a known cause of BMJ on the infantile side, but the responsible components of br...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31822f242e
更新日期:2011-11-01 00:00:00
abstract::We report a study on the effect of herpes simplex virus 1 (HSV-1) infection on apoptosis of neutrophils from both adults and neonates and present evidence showing that HSV-1 enhances apoptosis in neonatal, but not adult, neutrophils. HSV-1 enhanced the expression of both Fas and Fas ligand on the surface of neonatal n...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000191816.57544.b4
更新日期:2006-01-01 00:00:00
abstract::Twelve critically ill neonates mechanically ventilated for respiratory failure (mean weight 1.33 kg, mean gestation 31 wk) were studied to quantitate the effects of radiant power from a radiant warming device, body weight, and body surface area on insensible water loss. Radiant power density (Mw/cm2) was measured usin...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198112000-00008
更新日期:1981-12-01 00:00:00
abstract:INTRODUCTION:The Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) serves 50% of infants and 25% of preschool-aged children in the United States and collects height and weight measurements from eligible children every 6 mo, making WIC data a valuable resource for studying childhood growth an...
journal_title:Pediatric research
pub_type: 杂志文章,多中心研究
doi:10.1038/pr.2011.37
更新日期:2012-03-01 00:00:00