Abstract:
:In the present study, we evaluated the effectiveness of a levamisole-based adjuvant (ADL) to enhance the ability of the Ag85B-ESAT6 fusion protein to boost immune responses after primary vaccination with recombinant bacillus Calmette-Guerin (rBCG) in Balb/c mice. The results were compared with that of the control adjuvant formulation of dimethyl dioctadecylammonium bromide (DDA) and monophosphoryl lipid A (MPL), which has previously been shown to induce T-helper type 1 (Th1)-biased responses. Enzyme-linked immunospot (ELISPOT) assay with Ag85B and ESAT6 derived peptides corresponding to CD4+ and CD8+ T cell restricted epitopes and cell surface immunostaining indicated that Ag85B-ESAT6/ADL predominantly triggered activation of CD4+ T cells. Functional CD8+ T cells with interferon (IFN)-γ production or cytotoxicity were undetectable all vaccinated mice. The ADL adjuvant modified T-helper (Th) subtypes by up-regulating multiple signature cytokines. Furthermore, profiles of the immunoglobulin G (IgG) subtypes indicated ADL enhanced the secretion of Th1-associated IgG2a antibodies and decreased the yield of Th2-associated IgG1 subtype. These observations suggest that the ADL adjuvant formulated with a protein booster may induce Th1-biased cellular and humoral immune responses to primary vaccination with a live attenuated bacterial TB vaccine.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
You Q,Wu Y,Jiang D,Wu Y,Wang C,Wei W,Yu X,Zhang X,Kong W,Jiang Cdoi
10.3109/08820139.2012.658940subject
Has Abstractpub_date
2012-01-01 00:00:00pages
412-28issue
4eissn
0882-0139issn
1532-4311journal_volume
41pub_type
杂志文章abstract::A. actinomycetemcomitans is a facultative Gram-negative coccobacillus which has been implicated in the etiology and pathogenesis of localized juvenile periodontitis and has also been recognized for its potential to cause serious extraoral infections, particularly endocarditis. The polymorphonuclear neutrophil has been...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820138909112237
更新日期:1989-01-01 00:00:00
abstract::Prolonged survival of C57BL/6 (B6) mice bearing syngenic EL-4 leukemia cells resulted from immunization with irradiated EL-4 cells on the day of inoculation of live leukemia cells. No prolongation of survival was observed if the irradiated cells were injected 6 or 13 days after live cell inoculation. Protection also w...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138509022676
更新日期:1985-12-01 00:00:00
abstract::In this study we examined a panel of CD4+ antigen specific/MHC restricted T cell clones for their ability to secrete IL-2, IL-4, and IFN-gamma upon stimulation with con A, three lymphokines which are diagnostic for the TH1 and TH2 subtypes of helper T cells. Eight of the twelve clones we analyzed did not fit the class...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109054925
更新日期:1991-02-01 00:00:00
abstract::Results of recent studies indicated that a monoclonal ART18 antibody recognizes the receptor sites or closely related structures for IL-2 on activated rat T-cells. The major histocompatibility complex (MHC) class II (Ia) antigens (RT1B and RT1D in rat), detected with OX6 antibody, and OX17 antibody, may be present on ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709087087
更新日期:1987-06-01 00:00:00
abstract::We tested by Western blot several thousand antibody-secreting human cell lines immortalized by hybridoma fusion or Epstein-Barr virus transformation of peripheral blood lymphocytes from patients with systemic lupus erythematosus or mixed connective tissue disease. The blots utilized total human Jurkat cell extract as ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809041420
更新日期:1988-06-01 00:00:00
abstract::CD44 comprises a family of type I transmembrane glycoproteins that is expressed on a wide range of cells including those of epithelial, lymphoid and myeloid lineage. Although expression of CD44 in the small intestine is typically localised in the crypts of Lieberkuhn, we have reported the expression of CD44 on mature,...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820130009060867
更新日期:2000-08-01 00:00:00
abstract::The purpose of this presentation was to demonstrate that the red blood cell is not a hapless sac of hemoglobin and that much research is still needed for better understanding of its complexities. After a brief historical introduction the following subjects are presented: 1). Phosphofructokinase is the rate limiting st...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139509062760
更新日期:1995-01-01 00:00:00
abstract::Although the T cell costimulatory molecules CD2 and CD28 are enriched within the immunological synapse (IS), it has been suggested that costimulatory molecules need not be localized to the contact site between a T cell and an antigen-presenting cell (APC) in order to costimulate T cell activation. To determine whether...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701555035
更新日期:2008-01-01 00:00:00
abstract::Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically s...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109082633
更新日期:1991-08-01 00:00:00
abstract::The susceptibility of cataract Shionogi (CTS) mice as young as 8 to 10 weeks of age to passive anaphylactic shock mediated by anti-benzylpenicilloyl IgE and IgG1 monoclonal antibodies was compared with those of other strains of the same age including sister strains such as nonobese-diabetic (NOD) and nonobese-nondiabe...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109026232
更新日期:1991-06-01 00:00:00
abstract:BACKGROUND:The objective of this study was to investigate serum levels of immunosuppressive cytokines TGF beta 1 and VEGF and count of immune cells in peripheral blood in stage II and III colorectal cancer patients. METHODS:Blood samples were collected from 22 colorectal patients and 25 healthy controls before the sta...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2018.1480030
更新日期:2018-10-01 00:00:00
abstract::The phenomenon of apoptosis observed in lymphoid cells from HIV+ subjects is an important factor contributing to their massive depletion. Several studies have identified nitric oxide (NO) as one of the molecules involved in the apoptosis phenomenon observed during HIV infection. It has been shown that HIV-derived gp12...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-100108163
更新日期:2001-11-01 00:00:00
abstract::Cortical (immature) thymocytes are widely reported to express intermediate to high levels of receptors for the lectin, peanut agglutinin (PNA). Light-scatter studies of murine fetal thymocytes stained with PNA or anti-mouse CD4 and CD8 monoclonal antibodies indicated, however, that the most immature CD4-8- (DN) thymoc...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139309084180
更新日期:1993-12-01 00:00:00
abstract::A rapid, quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of Chagas' disease is described. The ELISA was standardized with the Falcon assay screening test (F.A.S.T.) system using a purified Trypanosoma cruzi epimastigote fraction (PEF) obtained by ion exchange chromatography. The ELISA te...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139809070892
更新日期:1998-01-01 00:00:00
abstract::Factors that suppress natural killer (NK) cell activity were examined in a random sample of 73 schizophrenic patients. NK activity in these patients were compared with 25 healthy age, sex and race matched controls. The mean percent of NK activity was 21% in the schizophrenic group compared with 30% percent in the cont...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139409066822
更新日期:1994-08-01 00:00:00
abstract::Exposure to laboratory animals poses a hazard for development of occupational allergy. Identification of antigenic determinants of allergenic proteins may be valuable for immunotherapeutic purposes. Overlapping peptides of the major allergen in rat urine, Rat n 1.02, corresponding to the protein alpha2u-globulin were ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139909062266
更新日期:1999-09-01 00:00:00
abstract::The rat small intestinal epithelial cell (enterocyte) has an average life span of 48h. Undifferentiated stem cells in the lower crypt region undergo division and differentiation into enterocytes as the cell moves on a basement membrane up the villus to reach the tip and be extruded into the lumen. The mechanism of thi...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138909112253
更新日期:1989-01-01 00:00:00
abstract::Food allergy is an important and common health issue, and there is a need to identify and characterize the sensitizing mechanisms. One of the common causes of food allergy is ovalbumin (OVA), a dietary antigen from eggs. We hypothesized that OVA-induced food allergy in the gut involves the activation of the chemokine ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120027682
更新日期:2004-02-01 00:00:00
abstract::Monocyte chemoattractant protein 1 (MCP-1) plays an important role in the development of periodontitis. The purpose of this investigation was to evaluate association of the MCP-1 gene polymorphism with chronic periodontitis in patients with end-stage renal disease (ESRD). One hundred fifty ESRD patients with chronic ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1702052
更新日期:2020-11-01 00:00:00
abstract::The bacterial superantigen Staphylococcal enterotoxin-A (SEA), produced by some strains of Staphylococcus aureus, causes proliferation of cytotoxic T-lymphocytes and cytokine production in vivo. SEA has been shown to be highly efficient for antibody-targeted superantegen immunotherapy for different tumor models. A can...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120003218
更新日期:2002-02-01 00:00:00
abstract::Mice were immunized with 14 free (i.e. not conjugated to any carrier) synthetic peptides representing the entire human hemoglobin alpha-chain. Antibodies against each peptide were determined using solid phase radioimmunoassay, both with free peptides and peptides coupled to a protein carrier as the coating antigen. It...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138909050764
更新日期:1989-08-01 00:00:00
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journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-100105064
更新日期:2001-08-01 00:00:00
abstract::Flow cytometric analyses of immune cell proliferation, differentiation, and function are limited by the number of different fluorochromes that can be resolved simultaneously. Additional colors to expand functional analytic capability will facilitate higher dimensional analyses of heterogeneous cell populations by basi...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701712461
更新日期:2007-01-01 00:00:00
abstract::Many proteins of various origins are able to form complexes with Immunoglobulin G using reaction sites formed by residues of constant domains. Studies of IgG complexes of non-immune nature are important as biologically active proteins could escape from the circulation forming complexes with IgG as well as with other s...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701854339
更新日期:2008-01-01 00:00:00
abstract::Objectives: Type 1 diabetes mellitus (T1D) has been disclosed to be associated with an elevated risk of cardiovascular disease (CVD), as well as increased risks of losing bone mass and progression of osteoporosis (OP). Osteoprotegerin (OPG), as a decoy receptor, has been demonstrated to play a critical role in bone me...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1710531
更新日期:2021-02-01 00:00:00
abstract::We examined the blocking ability of poly(vinyl alcohol) (PVA) in enzyme immunoassays by coating polystyrene microtiter wells with PVA of different molecular weights (MW) and percent hydrolysis (%Hyd). Blocking ability was measured by the differences in non-specific binding of an anti-rabbit IgG-horseradish peroxidase ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139409066836
更新日期:1994-11-01 00:00:00
abstract::The aim of the study was to assess whether genotypes in the Toll-like receptor 4 gene and in the promoter of the interleukin-18 gene are associated with the susceptibility to chronic periodontitis. 108 chronic periodontitis patients and 76 controls were genotyped for c.896A>G/1196C>T (TLR4 gene) and for c.-368G>C/ c.-...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130902846290
更新日期:2009-01-01 00:00:00
abstract::Competitive binding to polystyrene-bound antigens showed that human rye Group I (rye I)-specific IgG recognize the same three distinct epitopes of rye I as three monoclonal antibodies directed against the same antigens. Anti-idiotypic antibodies against one of the monoclonal antibodies (290A-167) inhibit completely th...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138609036364
更新日期:1986-12-01 00:00:00
abstract::The role of innate immunity, e.g., complement activation and cytokine release in the hemodynamic alterations in the course of human septic shock is largely unknown. We prospectively studied 14 consecutive septic shock patients with a pulmonary artery catheter in place. For 3 days after admission, hemodynamic variables...
journal_title:Immunological investigations
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
abstract::The effect of two Chinese traditional drugs, Dang Gui injection prepared from Angelica sinensis and C 21 Ester glucoside (GB) extracted from Cynanchus auriculatus on in vitro production of IL-2 has been studied. The IL-2 was produced by Con A stimulation of mouse spleen mononuclear cells. The IL-2 activity was assayed...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709030565
更新日期:1987-04-01 00:00:00