Comparison of peptide-coating conditions in solid phase plate assays for detection of anti-peptide antibodies.

abstract：:Food allergy is an important and common health issue, and there is a need to identify and characterize the sensitizing mechanisms. One of the common causes of food allergy is ovalbumin (OVA), a dietary antigen from eggs. We hypothesized that OVA-induced food allergy in the gut involves the activation of the chemokine ...

journal_title：Immunological investigations

authors： Lee JB,Matsumoto T,Shin YO,Yang HM,Min YK,Timothy O,Bae SJ,Quan FS

更新日期：2004-02-01 00:00:00

abstract：:In order to characterize the immune response to BK virus, a human polyomavirus containing dsDNA and host cell histones, we followed the appearance of antibodies in five outbred rabbits after intravenous inoculation with purified infectious BK virus without any adjuvant. The animals were followed for 15 weeks after the...

journal_title：Immunological investigations

authors： Fredriksen K,Traavik T,Flaegstad T,Rekvig OP

更新日期：1990-04-01 00:00:00

abstract：:Inoculation of metallic tin powder into Lewis rats resulted in marked enlargement of the draining lymph nodes. Plasma cells were the major contributors to the increase in cell mass. Certain immunologic aspects of this plasma cell lymphadenopathy were investigated. Immunoglobulin isotypes were distinguished and quantit...

journal_title：Immunological investigations

authors： Levine S,Saad A,Rappaport I

更新日期：1987-05-01 00:00:00

abstract：:Infections are a major cause of illness and death amongst elderly people. Peripheral blood CD8+ T lymphocytes --which play a crucial role in host defence against viral infections--, are divided in subsets based upon the expression of several cell and activation markers. In senescence changes and variations in peripher...

journal_title：Immunological investigations

authors： Esparza B,Rodriguez A,Puente M,Sabino E,Merino F

更新日期：1998-07-01 00:00:00

abstract：:Corneal transplant is the most common solid tissue transplant in humans. Advances in microsurgical techniques, eye banking and the use of corticosteroids have improved the success of corneal transplants. Over 65,000 corneal transplants are being performed worldwide annually. Most of these transplants are performed in ...

journal_title：Immunological investigations

authors： Kumar V,Kumar A

更新日期：2014-01-01 00:00:00

abstract：UNLABELLED:Cystic fibrosis (CF) lung disease is characterized by dysregulated inflammatory response in the airways. CD4(+)CD25(+) regulatory T cells play a crucial role in maintaining the immune homeostasis. However their role in the disease pathogenesis of CF remains unexplored. AIM:To determine the percentage of cir...

journal_title：Immunological investigations

authors： Anil N,Singh M

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:Grass pollen is a major cause of allergy throughout the world. The only treatment targeting the causes and not only the symptoms of allergy is specific immunotherapy (IT). A number of controlled trials demonstrated the efficacy of IT in grass pollen allergic subjects, most using extracts of multiple grasses ...

journal_title：Immunological investigations

authors： Marcucci F,Sensi L,Di Cara G,Incorvaia C,Puccinelli P,Scurati S,Frati F

更新日期：2010-01-01 00:00:00

abstract：:Woodchucks infected with woodchuck hepatitis virus (WHV) represent the closest natural animal model to study the immunopathogenesis of liver injury caused by essentially noncytopathic, highly human specific hepatitis B virus (HBV). The importance of antiviral T cell response in induction of hepatitis and in control of...

journal_title：Immunological investigations

authors： Gujar SA,Michalak TI

更新日期：2005-01-01 00:00:00

abstract：:The objective of this study was to analyze how Staphylococcus epidermidis SCV and WT strains manipulate the PI3K/Akt/mTOR signaling pathway. Six S. epidermidis strains with normal phenotype (WT) and six S. epidermidis strains with SCV phenotype were isolated in parallel from six patients with the prosthetic hip joint ...

journal_title：Immunological investigations

authors： Magryś A,Bogut A,Kiełbus M,Olender A

更新日期：2018-04-01 00:00:00

abstract：:An enzyme-linked immunoassay (EIA) is described and evaluated which quantitates human antibodies to serotype specific S. pneumoniae polysaccharide (PnPs) in human sera. Based on the observations previously described by Koskela (1), native PnPs are used as coating antigens and sera are absorbed with a soluble pneumococ...

journal_title：Immunological investigations

authors： Quataert S,Martin D,Anderson P,Giebink GS,Henrichsen J,Leinonen M,Granoff DM,Russell H,Siber G,Faden H,Barnes D,Madore DV

更新日期：2001-08-01 00:00:00

abstract：:Conventional plate ML-ELISA technique for diagnosis of recent cases of amoebiasis was converted into a simple polyvinyl chloride strip multilayer-enzyme linked immunosorbent assay (PVC strip ML-ELISA). This report details the development and preliminary characterization of the PVC strip ML-ELISA method for Entamoeba h...

journal_title：Immunological investigations

authors： Agarwal RK,Rawat R,Malaviya B,Das SR

更新日期：1991-12-01 00:00:00

abstract：:Exposure to laboratory animals poses a hazard for development of occupational allergy. Identification of antigenic determinants of allergenic proteins may be valuable for immunotherapeutic purposes. Overlapping peptides of the major allergen in rat urine, Rat n 1.02, corresponding to the protein alpha2u-globulin were ...

journal_title：Immunological investigations

authors： Bayard C,Siddique AB,Berzins K,Troye-Blomberg M,Hellman U,Vesterberg O

更新日期：1999-09-01 00:00:00

abstract：:A group of 11 medicinal plants, including Lavandula pubescens, Trigonella foenugricium, Salsola schweinforthi, Calligonum comosum, Silene succulenta, Silene villosa, Bogonvillea glabra, Cakile maritime, Gomphrene celesoids, Mirabilis jalaba, and Silene nocturna growing in Egypt, were extracted and examined for their i...

journal_title：Immunological investigations

authors： Ghonime M,Emara M,Shawky R,Soliman H,El-Domany R,Abdelaziz A

更新日期：2015-01-01 00:00:00

abstract：:Alveolar macrophages (AM) freshly obtained by bronchoalveolar lavage suppressed significantly, in a dose-dependent fashion, lung interstitial lymphocytes cytotoxicity against the NK-sensitive target cells, YAC-1. Kinetic experiments revealed that AM-mediated suppression of NK activity was seen following short-term inc...

journal_title：Immunological investigations

authors： Lemaire I,St-Jean M

更新日期：1990-02-01 00:00:00

abstract：:Two cell lines secreting Paul-Bunnell antibodies were established by means of Epstein-Barr virus-induced immortalization of B lymphocytes from patients with infectious mononucleosis. Progressive loss of antibody production, most probably due to a shutdown of antibody secretion by individual cells was, however, observe...

journal_title：Immunological investigations

authors： Wasik M,Milgrom F

更新日期：1986-06-01 00:00:00

abstract：:Flow cytometric analyses of immune cell proliferation, differentiation, and function are limited by the number of different fluorochromes that can be resolved simultaneously. Additional colors to expand functional analytic capability will facilitate higher dimensional analyses of heterogeneous cell populations by basi...

journal_title：Immunological investigations

authors： Bantly AD,Gray BD,Breslin E,Weinstein EG,Muirhead KA,Ohlsson-Wilhelm BM,Moore JS

更新日期：2007-01-01 00:00:00

abstract：:Spleen cells of C57BL/6 mice injected with corticosterone were compared with cells from mice injected with saline in their ability to respond to the mitogens PHA, Con A, and LPS. Both unseparated cells and cells separated into T-enriched and B-enriched fractions were studied. After either seven daily injections or fou...

journal_title：Immunological investigations

authors： Stevenson JR,Kreiling JA,Taylor R

更新日期：1989-10-01 00:00:00

abstract：:Many proteins of various origins are able to form complexes with Immunoglobulin G using reaction sites formed by residues of constant domains. Studies of IgG complexes of non-immune nature are important as biologically active proteins could escape from the circulation forming complexes with IgG as well as with other s...

journal_title：Immunological investigations

authors： Nezlin R,Bengtsson AA

更新日期：2008-01-01 00:00:00

abstract：:Anti-galactocerebroside (GalC) antibodies have been reported to inhibit myelin formation, cause demyelination, and block HIV-I infection of neural cells. We examined the binding of 3 monoclonal and polyclonal anti-GalC antibodies to a panel of purified glycolipids by ELISA and by an immunospot assay on nitrocellulose ...

journal_title：Immunological investigations

authors： McAlarney T,Ogino M,Apostolski S,Latov N

更新日期：1995-05-01 00:00:00

abstract：OBJECTIVE:The aim of this study was to determine whether caspase recruitment domain-containing protein 8 (CARD8) rs2043211 polymorphism was associated with susceptibility to inflammatory bowel disease (IBD). METHODS:Relevant studies were searched using PubMed and Embase up to February 2014. A meta-analysis was conduct...

journal_title：Immunological investigations

authors： Liu J,Liu YY,Liu J,Li BZ,Cen H,Xu WD,Leng RX,Pan HF,Ye DQ

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Adult chronic immune thrombocytopenic purpura (chronic ITP) is an autoimmune multifactorial bleeding disorder that occurs because of enhanced peripheral platelet destruction. Treatment decisions can be challenging because the goal of treatment is to prevent severe bleeding, but the risk of bleeding can be di...

journal_title：Immunological investigations

authors： El Ghannam D,Fawzy IM,Azmy E,Hakim H,Eid I

更新日期：2015-01-01 00:00:00

abstract：:Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically s...

journal_title：Immunological investigations

authors： Matta KL,Piskorz CF,Abbas SA,Diakun KR

更新日期：1991-08-01 00:00:00

abstract：:The aim of this study was to evaluate the possible association of IL12B gene polymorphisms with serum levels of IL-12p40, IL-23 and genetic susceptibility to rheumatoid arthritis (RA) in the Bulgarian population. Genotyping for IL12Bpro (rs17860508) and IL12B A/C - 3' UTR (rs3212227) polymorphisms was performed by pol...

journal_title：Immunological investigations

authors： Manolova I,Ivanova M,Vasilev G,Stoilov R,Miteva L,Stanilova S

更新日期：2020-02-01 00:00:00

abstract：:The relationship between antibody avidity, measured by the dissociation of the antigen-antibody bond in antigen excess, and antibody affinity was studied. Complexes of radiolabelled antigen and antibody of known affinity were prepared in vitro and allowed to stand for seven days to reach equilibrium. Then nonlabelled ...

journal_title：Immunological investigations

authors： Griswold WR

更新日期：1987-04-01 00:00:00

abstract：OBJECTIVE:This study aimed to examine the association of three functional IRF5 rs10954213, rs3757385, and rs41298401 polymorphisms with susceptibility to unexplained recurrent pregnancy loss (RPL) among Iranian women from south of Iran. METHODS:176 women with unexplained RPL and 173 healthy postmenopausal controls wer...

journal_title：Immunological investigations

authors： Amiri Jahromi R,Nasiri M,Jahromi AR

更新日期：2017-01-01 00:00:00

abstract：:Studies demonstrated that deficiency in 17β-estradiol (E2) in postmenopausal women influences their immune system. However, few studies have reported alterations in immunologic presentation during nonnatural menopause in young females. Here we compared the differences in immune response between young C57BL/6N mice wit...

journal_title：Immunological investigations

authors： Shao MJ,Zhu YJ,Qiu YE,Hu M,He YQ

更新日期：2017-04-01 00:00:00

abstract：:There is a continuing need for alternatives to current human adjuvants. Recombinant protein vaccines, which target antigen to human Fc gamma receptor type I (hFcgammaRI) on hFcgammaRI-expressing antigen presenting cells, provide one potential alternative. Using a recombinant anti-hFcgammaRI-antigen fusion protein and ...

journal_title：Immunological investigations

authors： Adamova E,Walsh MC,Gosselin DR,Hale K,Preissler MT,Graziano RF,Gosselin EJ

更新日期：2005-01-01 00:00:00

abstract：:CD44 comprises a family of type I transmembrane glycoproteins that is expressed on a wide range of cells including those of epithelial, lymphoid and myeloid lineage. Although expression of CD44 in the small intestine is typically localised in the crypts of Lieberkuhn, we have reported the expression of CD44 on mature,...

journal_title：Immunological investigations

authors： Reid SD,Pockley AG

更新日期：2000-08-01 00:00:00

abstract：:The role of ICAM-1 in contact activation of the bronchial epithelial cells is elucidated. Direct contact between epithelial cells and leukocytes is required to change transepithelial electrical resistance (TER) of the epithelium. Migration of human neutrophils across the layers of cultured human airway epithelial cell...

journal_title：Immunological investigations

authors： Choi H,Fleming NW,Serikov VB

更新日期：2007-01-01 00:00:00

abstract：:Studies presented examine the origin of IgA plasma cells in B lineage chimeric mice constructed by reconstituting lethally irradiated mice with a mixture of syngeneic bone marrow cells and peritoneal cells from Ig heavy chain allotype congenic donors. In these mice, essentially all B cells in spleen and Peyer's patche...

journal_title：Immunological investigations

authors： Kroese FG,Butcher EC,Stall AM,Herzenberg LA

更新日期：1989-01-01 00:00:00