Abstract:
:The relationship between antibody avidity, measured by the dissociation of the antigen-antibody bond in antigen excess, and antibody affinity was studied. Complexes of radiolabelled antigen and antibody of known affinity were prepared in vitro and allowed to stand for seven days to reach equilibrium. Then nonlabelled antigen in one hundred fold excess was added to dissociate the complexes. After an appropriate incubation the fraction of antigen bound to antibody was measured by the ammonium sulfate precipitation method. The dissociation index was the fraction bound in the experimental sample divided by the fraction bound in the control. The correlation coefficient between the dissociation index and the antibody binding constant was 0.92 for early dissociation and 0.98 for late dissociation. The regression equation relating the binding constant to the dissociation index was K = 6.4(DI) + 6.25, where DI is the late dissociation index and K is the logarithm to the base 10 of the binding constant. There is a high correlation between avidity and affinity of antibody. Antibody affinity can be estimated from avidity data. The stability of antigen-antibody complexes can be predicted from antibody affinity.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
Griswold WRdoi
10.3109/08820138709030567subject
Has Abstractpub_date
1987-04-01 00:00:00pages
97-106issue
2eissn
0882-0139issn
1532-4311journal_volume
16pub_type
杂志文章abstract::Th17 cells of the intestine and colon can produce several important cytokines during mucosal inflammation. However, few studies have focused on the role of IL-26 in intestinal inflammations. Colonic epithelial cells express receptors for IL-26, and this cytokine has been shown to induce the HT-29 colonic epithelial ce...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1594247
更新日期:2019-11-01 00:00:00
abstract::Bromelain has been reported to have anti-inflammatory and immunomodulatory effects. However, the anti-inflammatory mechanism of bromelain is unclear. Therefore, we investigated the effect of bromelain on cytokine production from lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) and mo...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130802083622
更新日期:2008-01-01 00:00:00
abstract::We investigated the functional and subset surface marker characteristics of supramammary lymph node T cell populations at sites proximal and distal to the mammary region of goats repeatedly injected with heat-treated Staphylococcus aureus antigen (HK-SAC). Flow cytometric studies showed quantitative differences in CD4...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139209066184
更新日期:1992-04-01 00:00:00
abstract::The contribution of interleukin-2 (IL-2)-responsive bystander cells to the proliferative responses of human peripheral blood T lymphocytes to antigens used for sensitization such as Purified Protein Derivative (PPD), Tetanus toxoid (T T) and Influenza virus was investigated. Marked proliferation of the unfractionated ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809055720
更新日期:1988-03-01 00:00:00
abstract::There has been a dramatic increase recently in the number of reports of septic episodes associated with both red cell and platelet concentrate transfusions. These reports suggest that transfusion-associated septic reactions may occur as often as 1 per 4000 platelet transfusions, however, the true incidence of the bact...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139509062770
更新日期:1995-01-01 00:00:00
abstract::Adenoid hypertrophy is the most common cause of upper airway obstruction and sleep-disordered breathing in children, yet its pathogenesis remains unclear. The identification of the novel helper T cell subsets, Th17 cells and regulatory T cells (Tregs) could provide new insight into our understanding of the mechanisms ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2011.575426
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Loquat leaf is an herb that is commonly used in traditional Chinese medicine (TCM) for its anti-inflammatory properties. Numerous studies have demonstrated that Th17 cells play a fundamental role in mediating SLE pathological deterioration. In our study, we investigated the inhibitory effect of pentacyclic t...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1698599
更新日期:2020-08-01 00:00:00
abstract::DNA vaccine represents a powerful approach for prevention of avian H5N1 influenza infection. Yet, DNA vaccine-induced immune responses might be limited by the short duration of antigen expression. As a strategy to enhance adaptive immune responses elicited by a hemagglutinin 5 (H5) DNA vaccine, we explored the effect ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2014.988718
更新日期:2015-01-01 00:00:00
abstract::To investigate whether phospholipase D1 (PLD1) is involved in autoimmune damage to the eyes, we used Western blotting and immunohistochemistry to examine the expression and distribution of PLD1 in the retinas of Lewis rats with experimental autoimmune uveoretinitis (EAU). Western blot analysis showed that the level of...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The expression of peripheral antigens in the thymus, known as promiscuous gene expression, has been implicated in T cell tolerance and autoimmunity. Here we identified thymic epithelial cells (TECs) as the main cell type that expresses a diverse range of tissue-restricted antigens (TRAs). The TECs of a common autoimmu...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130801967841
更新日期:2008-01-01 00:00:00
abstract::The bacterial superantigen Staphylococcal enterotoxin-A (SEA), produced by some strains of Staphylococcus aureus, causes proliferation of cytotoxic T-lymphocytes and cytokine production in vivo. SEA has been shown to be highly efficient for antibody-targeted superantegen immunotherapy for different tumor models. A can...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120003218
更新日期:2002-02-01 00:00:00
abstract::The aim of this study was to evaluate the possible association of IL12B gene polymorphisms with serum levels of IL-12p40, IL-23 and genetic susceptibility to rheumatoid arthritis (RA) in the Bulgarian population. Genotyping for IL12Bpro (rs17860508) and IL12B A/C - 3' UTR (rs3212227) polymorphisms was performed by pol...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1622561
更新日期:2020-02-01 00:00:00
abstract::High altitude may be defined as elevations as low as 1500 meters to those as high as 8800 meters. Very few studies have been conducted so far at high altitude for the specific purpose of monitoring different immune parameters in humans. Military personnel and mountaineers may be required to perform in environmental ex...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820131003681144
更新日期:2010-01-01 00:00:00
abstract::Flow cytometric analyses of immune cell proliferation, differentiation, and function are limited by the number of different fluorochromes that can be resolved simultaneously. Additional colors to expand functional analytic capability will facilitate higher dimensional analyses of heterogeneous cell populations by basi...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701712461
更新日期:2007-01-01 00:00:00
abstract::Objectives: The aim of this study was to assess the genetic association between vascular endothelial growth factor (VEGF) gene polymorphisms and the risk of pre-eclampsia (PE).Methods: A systematic literature search of several databases (PubMed, Embase, and the China National Knowledge Infrastructure (CNKI)) was condu...
journal_title:Immunological investigations
pub_type: 杂志文章,meta分析
doi:10.1080/08820139.2019.1659812
更新日期:2020-02-01 00:00:00
abstract::Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically s...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109082633
更新日期:1991-08-01 00:00:00
abstract::Plasmodium falciparum CTL epitope minigenes containing HLA-A2 and HLA-B7 subtype supermotifs were cloned into a plasmid expression vector; this expression was measured in eight human HLA class I molecule specific cell lines. Three assays for in vitro antigen presentation analysis were developed to examine the cross-bi...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120019206
更新日期:2003-02-01 00:00:00
abstract::Identification and characterization of antigenic proteins of Setaria cervi (bovine filarial parasite) adults and microfilariae was done by immunoblotting technique using hyperimmune rabbit sera against S. cervi and Brugia malayi. The antigens recognized by these sera were detected by using 125I protein-A followed by a...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709030571
更新日期:1987-04-01 00:00:00
abstract:OBJECTIVE:Diabetic peripheral neuropathy (DPN) is one of late complications of diabetes mellitus. The aim of this study was to evaluate the association between variable number tandem repeat (VNTR) polymorphism in intron 3 of interleukin-4 gene and risk of DPN. METHODS:We examined 926 T2DM patients and 420 healthy cont...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1407334
更新日期:2018-02-01 00:00:00
abstract::In order to characterize the immune response to BK virus, a human polyomavirus containing dsDNA and host cell histones, we followed the appearance of antibodies in five outbred rabbits after intravenous inoculation with purified infectious BK virus without any adjuvant. The animals were followed for 15 weeks after the...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139009042033
更新日期:1990-04-01 00:00:00
abstract::Infections are a major cause of illness and death amongst elderly people. Peripheral blood CD8+ T lymphocytes --which play a crucial role in host defence against viral infections--, are divided in subsets based upon the expression of several cell and activation markers. In senescence changes and variations in peripher...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139809070903
更新日期:1998-07-01 00:00:00
abstract::The processing of antigenic peptides for presentation by MHC molecules to T cells, may depend upon the function of a second, consensus sequence in or near the T cell-presented epitope. One such processing-regulating sequence appears to be composed of amino acids Leu, Ile, Val, Phe, and Met recurring in a fashion to fo...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139309066191
更新日期:1993-02-01 00:00:00
abstract::Interleukin-31 (IL-31) has diverse biological functions. Increased IL-31 has been found in some skin and autoimmune diseases. There has been no study reporting the association between IL-31 and primary biliary cholangitis (PBC). This study was designed to determine serum IL-31 level and to explore its diagnostic value...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2020.1785490
更新日期:2020-07-02 00:00:00
abstract::In recent studies, using a swine model of single lung transplantation, we demonstrated that IRI alone increased MHC II expression in the host's peripheral T lymphocytes. The inhibition of increased MHC II expression with TCV-309, a specific platelet-activating factor (PAF) antagonist suggested that PAF might play a ro...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139909060857
更新日期:1999-07-01 00:00:00
abstract::Experimental autoimmune uveoretinitis (EAU) was induced in rats by immunization with bovine interphotoreceptor retinoid-binding protein (IRBP) and studied by immunohistochemistry. In general, the IRBP induction of inflammatory cellular components and expression of immune-related antigens on various non-lymphoid cells ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709055713
更新日期:1987-03-01 00:00:00
abstract::Electroeluted outer membrane proteins [(EOmp), (35-37 KDa, porins)] were highly immunogenic in New Zealand White rabbits. An ELISA peak titer of 51,200 to EOmp as compared to 6,400 for non-eluted outer membrane protein (Omp) was demonstrated. EOmp enhanced the antigenicity of Omp possibly due to epitopes which were ma...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139409087802
更新日期:1994-04-01 00:00:00
abstract::The relationships between polymorphisms in pro-inflammatory cytokines and the risk of inflammatory bowel disease (IBD) remain discrepant. Therefore, the authors conducted a meta-analysis to robustly explore relationships between polymorphisms in pro-inflammatory cytokines and the risk of IBD by integrating the results...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2020.1787438
更新日期:2020-08-26 00:00:00
abstract::DNA vaccines have been widely used to induce immune responses against molecular targets. In this study, we explored the possibility of using DNA vaccine combined with the immunosuppressant FK506 (tacrolimus) to antigen-specifically suppress unwanted immune responses and prevent autoimmune ovarian disease. To that end,...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2011.622828
更新日期:2012-01-01 00:00:00
abstract::It has become apparent that extreme environmental conditions of Antarctic continent alters many immune responses. The present study was conducted on 28th Indian Antarctic expeditioners. The investigations were carried out to explore the effect of multiple stresses like isolation, cold and UV exposure on human immunity...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2010.494194
更新日期:2010-01-01 00:00:00
abstract::Interleukin (IL)-10 response is associated with mortality in patients with sepsis. IL-10 is primarily produced by monocytes and type 2 T helper (Th2) cells. The aim of this study was to investigate differences in IL-10 production between monocytes and Th2 cells in patients with sepsis. Forty patients with sepsis and 3...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1288237
更新日期:2017-05-01 00:00:00