Abstract:
:The processing of antigenic peptides for presentation by MHC molecules to T cells, may depend upon the function of a second, consensus sequence in or near the T cell-presented epitope. One such processing-regulating sequence appears to be composed of amino acids Leu, Ile, Val, Phe, and Met recurring in a fashion to form a longitudinal, hydrophobic strip when the excised peptide is coiled as an alpha-helix. Such a hydrophobic strip-of-helix may: (a) scavenge peptides from lumens onto lipid membranes of digestion vesicles, (b) stabilize peptides there as protease-resistant helices, (c) specify recognition by the antigenic peptide-binding sites of chaperonin proteins, transmembranal transporters, or MHC molecules. By circular dichroism and electron paramagnetic resonance, we demonstrated that peptides with recurrent hydrophobic residues potentially forming longitudinal strips adsorbed to, and partially coiled as helices on, di-O-hexadecyl, D-L-alpha-phosphatidylcholine (DHPC) vesicles. Cathepsin B or cathepsin D cleavages of three such peptides were identified. With either enzyme, it made no significant difference whether a peptide substrate was in solution or bound to vesicles in terms of efficiency and specificity of peptide bond cleavages. We conclude that protease resistance, per se, of membrane-adsorbed, helically coiled peptides is not a major factor in the selection for T cell presentation of epitopes in peptides which have a motif with a longitudinal hydrophobic strip.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
Goldschmidt TG,Reyes VE,You G,Nelson DJ,Reisert PS,Anderson J,Mole J,Humphreys REdoi
10.3109/08820139309066191subject
Has Abstractpub_date
1993-02-01 00:00:00pages
25-40issue
1eissn
0882-0139issn
1532-4311journal_volume
22pub_type
杂志文章abstract::Competitive binding to polystyrene-bound antigens showed that human rye Group I (rye I)-specific IgG recognize the same three distinct epitopes of rye I as three monoclonal antibodies directed against the same antigens. Anti-idiotypic antibodies against one of the monoclonal antibodies (290A-167) inhibit completely th...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138609036364
更新日期:1986-12-01 00:00:00
abstract::We investigated the functional and subset surface marker characteristics of supramammary lymph node T cell populations at sites proximal and distal to the mammary region of goats repeatedly injected with heat-treated Staphylococcus aureus antigen (HK-SAC). Flow cytometric studies showed quantitative differences in CD4...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139209066184
更新日期:1992-04-01 00:00:00
abstract::Thrombocytopenia as a complicating event in human immunodeficiency infection is a rather common hematological disorder and in many aspects resembles idiopathic(immune) thrombocytopenic purpura (ITP). Consequently, treatment of severe cases is similar to treating ITP, but steroids should be administered with particular...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139109050791
更新日期:1991-04-01 00:00:00
abstract::Vitamin D deficiency and vitamin D receptor (VDR) gene polymorphisms have been reported in autoimmune diseases. However, their role in Behçet's disease (BD) and scleroderma remains inconclusive. Our aim was to evaluate vitamin D receptor (ApaI, TaqI) gene polymorphisms in relation to Behçet's disease and scleroderma i...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1180302
更新日期:2016-08-01 00:00:00
abstract::In the present study, we evaluated the effectiveness of a levamisole-based adjuvant (ADL) to enhance the ability of the Ag85B-ESAT6 fusion protein to boost immune responses after primary vaccination with recombinant bacillus Calmette-Guerin (rBCG) in Balb/c mice. The results were compared with that of the control adju...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2012.658940
更新日期:2012-01-01 00:00:00
abstract::Anti-galactocerebroside (GalC) antibodies have been reported to inhibit myelin formation, cause demyelination, and block HIV-I infection of neural cells. We examined the binding of 3 monoclonal and polyclonal anti-GalC antibodies to a panel of purified glycolipids by ELISA and by an immunospot assay on nitrocellulose ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139509066860
更新日期:1995-05-01 00:00:00
abstract::IL-2 production by the phytohemaglutinin (PHA)-stimulated mononuclear cells (MNC) of peripheral blood (PB) and synovial fluid (SF) from patients with rheumatoid arthritis (RA), other arthritic diseases (OAD) and age-matched normal controls were studied, and the activity of IL-2 inhibitor in sera of studied subject was...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139509060720
更新日期:1995-11-01 00:00:00
abstract::Hematological disorders like Aplastic anemia are quite frequent in Pakistan. Human leukocyte antigen (HLA) system, have been implicated in the development of Aplastic anemia in various population-based studies, The aim of this study was to determine the role of the HLA Class I and Class II alleles in genetic susceptib...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820130903271415
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Association studies of genes encoding cytokines that play an important role in inflammatory response represent one approach to finding type 1 diabetes (T1D) disease genes. The aim of this study was to investigate the association of single nucleotide polymorphisms (SNPs) within cytokine genes with T1D in a co...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1416398
更新日期:2018-04-01 00:00:00
abstract::Corneal transplant is the most common solid tissue transplant in humans. Advances in microsurgical techniques, eye banking and the use of corticosteroids have improved the success of corneal transplants. Over 65,000 corneal transplants are being performed worldwide annually. Most of these transplants are performed in ...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139.2014.910024
更新日期:2014-01-01 00:00:00
abstract::DNA vaccine represents a powerful approach for prevention of avian H5N1 influenza infection. Yet, DNA vaccine-induced immune responses might be limited by the short duration of antigen expression. As a strategy to enhance adaptive immune responses elicited by a hemagglutinin 5 (H5) DNA vaccine, we explored the effect ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2014.988718
更新日期:2015-01-01 00:00:00
abstract::Conventional plate ML-ELISA technique for diagnosis of recent cases of amoebiasis was converted into a simple polyvinyl chloride strip multilayer-enzyme linked immunosorbent assay (PVC strip ML-ELISA). This report details the development and preliminary characterization of the PVC strip ML-ELISA method for Entamoeba h...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109026242
更新日期:1991-12-01 00:00:00
abstract:OBJECTIVE:To study the relation between CD226 rs763361 gene polymorphism and CD226 serum level and to evaluate their role in susceptibility and disease activity of RA in a cohort of Egyptian individuals. METHODS:The serum level of CD226 was measured using a suitable ELISA kit and the CD226 rs763361 gene polymorphism w...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2018.1423570
更新日期:2018-04-01 00:00:00
abstract::The purpose of this presentation was to demonstrate that the red blood cell is not a hapless sac of hemoglobin and that much research is still needed for better understanding of its complexities. After a brief historical introduction the following subjects are presented: 1). Phosphofructokinase is the rate limiting st...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139509062760
更新日期:1995-01-01 00:00:00
abstract::Seven commercially available intravenous immunoglobulins (IVIG) preparations, a gamma globulin prepared by ethanol fractionation, and an experimental IgG isolated by a chromatographic procedure were compared in several tests. Split products were present in preparations manufactured by procedures involving protease tre...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809055724
更新日期:1988-04-01 00:00:00
abstract::The aim of the study was to assess whether genotypes in the Toll-like receptor 4 gene and in the promoter of the interleukin-18 gene are associated with the susceptibility to chronic periodontitis. 108 chronic periodontitis patients and 76 controls were genotyped for c.896A>G/1196C>T (TLR4 gene) and for c.-368G>C/ c.-...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130902846290
更新日期:2009-01-01 00:00:00
abstract::Endomyocardial biopsy is generally used to quantify heart allograft rejection and guide immunotherapy. Biopsy, however, is invasive, costly, and risky. Since rejection requires lymphocyte activation, the purpose of this study was to assess alternative methods to evaluate rejection dynamics by investigating serum level...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139609059287
更新日期:1996-01-01 00:00:00
abstract::Immunological parameters previously shown to constitute an increased risk for progression towards AIDS have been observed in clinically asymptomatic individuals considered to be at risk for this syndrome. These parameters include severely decreased numbers of T helper cells (count below 400/mm3 blood for CD4+ cells we...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809055726
更新日期:1988-04-01 00:00:00
abstract::Spleen cells of C57BL/6 mice injected with corticosterone were compared with cells from mice injected with saline in their ability to respond to the mitogens PHA, Con A, and LPS. Both unseparated cells and cells separated into T-enriched and B-enriched fractions were studied. After either seven daily injections or fou...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138909045782
更新日期:1989-10-01 00:00:00
abstract::Electroeluted outer membrane proteins [(EOmp), (35-37 KDa, porins)] were highly immunogenic in New Zealand White rabbits. An ELISA peak titer of 51,200 to EOmp as compared to 6,400 for non-eluted outer membrane protein (Omp) was demonstrated. EOmp enhanced the antigenicity of Omp possibly due to epitopes which were ma...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139409087802
更新日期:1994-04-01 00:00:00
abstract::Each functional gene illustrates the complexity of genetic predisposition to disease; however, it is difficult to bring out these traits with reference to autoimmune diseases like type 1 diabetes (T1D). To find out the genetic contribution of CTLA-4 + 49A/G, CTLA-4 -318C/T and CD28 + 17T/C polymorphisms toward T1D, th...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1590395
更新日期:2019-08-01 00:00:00
abstract::Many proteins of various origins are able to form complexes with Immunoglobulin G using reaction sites formed by residues of constant domains. Studies of IgG complexes of non-immune nature are important as biologically active proteins could escape from the circulation forming complexes with IgG as well as with other s...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701854339
更新日期:2008-01-01 00:00:00
abstract::In the last 15 years, it has become apparent that ovarian cancer is recognized by the immune system, taking into account that T cell infiltration can be associated with increased overall survival. Several studies indicate that a correct combination of cluster of differentiation 8 and cluster of differentiation 4 T cel...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.1080/08820139.2016.1186689
更新日期:2016-11-01 00:00:00
abstract::The critical contribution of CD4+CD25+Foxp3+ T-regulatory cells (Treg) to immune suppression in the tumor microenvironment is well-established. Whereas the mechanisms that drive the generation and accumulation of Treg in tumors have been an active area of study, the information on their origin and population dynamics ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1220390
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:This study aimed to examine the association of three functional IRF5 rs10954213, rs3757385, and rs41298401 polymorphisms with susceptibility to unexplained recurrent pregnancy loss (RPL) among Iranian women from south of Iran. METHODS:176 women with unexplained RPL and 173 healthy postmenopausal controls wer...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1225759
更新日期:2017-01-01 00:00:00
abstract::The occurrence of apoptosis in cultured blood cells from HIV+ patients is well-documented. However, the relationship of this process to cytokine production is still undefined. We measured the production of TNF-beta by mitogen-stimulated PBLs from 33 HIV+ patients, while simultaneously assessing their development of ap...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139609059311
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:Loquat leaf is an herb that is commonly used in traditional Chinese medicine (TCM) for its anti-inflammatory properties. Numerous studies have demonstrated that Th17 cells play a fundamental role in mediating SLE pathological deterioration. In our study, we investigated the inhibitory effect of pentacyclic t...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1698599
更新日期:2020-08-01 00:00:00
abstract::VH4-34 gene encoded autoantibodies are elevated in systemic lupus erythematosus (SLE) and in other diseases associated with B-cell hyperproliferation/dysfunction. One of the autoantigens recognized by VH4-34-encoded antibodies are branched/linear poly N-acetyl lactosamine chains. Since the anti-carbohydrate response i...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2015.1015682
更新日期:2015-01-01 00:00:00
abstract::The phenomenon of apoptosis observed in lymphoid cells from HIV+ subjects is an important factor contributing to their massive depletion. Several studies have identified nitric oxide (NO) as one of the molecules involved in the apoptosis phenomenon observed during HIV infection. It has been shown that HIV-derived gp12...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-100108163
更新日期:2001-11-01 00:00:00
abstract::A group of 11 medicinal plants, including Lavandula pubescens, Trigonella foenugricium, Salsola schweinforthi, Calligonum comosum, Silene succulenta, Silene villosa, Bogonvillea glabra, Cakile maritime, Gomphrene celesoids, Mirabilis jalaba, and Silene nocturna growing in Egypt, were extracted and examined for their i...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2014.988720
更新日期:2015-01-01 00:00:00