Abstract:
:Cortical (immature) thymocytes are widely reported to express intermediate to high levels of receptors for the lectin, peanut agglutinin (PNA). Light-scatter studies of murine fetal thymocytes stained with PNA or anti-mouse CD4 and CD8 monoclonal antibodies indicated, however, that the most immature CD4-8- (DN) thymocyte subpopulation binds levels of PNA commonly described as PNAlo. Evaluation of the PNA binding characteristics of fetal thymocytes negative for the CD8 antigen confirmed the existence of a major population (approximately 20% of total cells) of CD4-8- PNAlo fetal thymocytes. The majority of these DN thymocytes were subsequently found to bind sub-agglutinating levels of PNA, similar to mature CD4+ or CD8+ single positive (SP) thymocytes. Given this information, an immunomodulating compound (2,3,7,8-tetrachlorodibenzo-p-dioxin; TCDD) known to produce a maturational delay in murine thymocytes was tested for a possible concurrent effect on thymocyte PNA lectin binding. A TCDD-induced increase in DN thymocytes was found to be paralleled by an increase of equal magnitude in PNAlo thymocytes. Taken together, these data provide evidence that acquisition of the PNA receptor may be a maturational event occurring during the DN stage of thymocyte ontogeny. Further, these results suggest that separation of thymocytes into mature (medullary) and immature (cortical) subpopulations by PNA agglutination may result in contamination of medullary cells by the most immature (DN) subpopulation of thymocytes.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
Holladay S,Blaylock B,Smith B,Luster Mdoi
10.3109/08820139309084180subject
Has Abstractpub_date
1993-12-01 00:00:00pages
517-29issue
8eissn
0882-0139issn
1532-4311journal_volume
22pub_type
杂志文章abstract::The complement system which is a critical mediator of innate immunity plays diverse roles in the neuropathogenesis of HIV-1 infection such as clearing HIV-1 and promoting productive HIV-1 replication. In the development of HIV-1 associated neurological disorders (HAND), there may be an imbalance between complement act...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1371891
更新日期:2017-11-01 00:00:00
abstract::The lectin from Amaranthus leucocarpus (ALL) is specific for N-acetyl-D-galactosamine and inhibits phagocytosis of Leishmania mexicana promastigotes in Balb/c mice peritoneal macrophages by 38%. The lipophosphoglycan (LPG) purified from L. mexicana inhibits penetration of promastigotes into peritoneal macrophages by 3...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::It has become apparent that extreme environmental conditions of Antarctic continent alters many immune responses. The present study was conducted on 28th Indian Antarctic expeditioners. The investigations were carried out to explore the effect of multiple stresses like isolation, cold and UV exposure on human immunity...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2010.494194
更新日期:2010-01-01 00:00:00
abstract::The antiviral activity of cell-associated, non-elutable recombinant human gamma interferon (rHuIFN-gamma) was neutralized by antibody. The neutralization of cell-associated rHuIFN-gamma was maximal through 2 h (60-100%) and declined through 8 h (20-40%). Concomitantly, the antiviral activity of cell-associated [Met-Gl...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139009052977
更新日期:1990-10-01 00:00:00
abstract:BACKGROUND:Grass pollen is a major cause of allergy throughout the world. The only treatment targeting the causes and not only the symptoms of allergy is specific immunotherapy (IT). A number of controlled trials demonstrated the efficacy of IT in grass pollen allergic subjects, most using extracts of multiple grasses ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820131003796876
更新日期:2010-01-01 00:00:00
abstract::The objectives were to determine the prevalence and clinical significance of anti-cyclic citrullinated peptide (anti-CCP) antibodies in patients with juvenile idiopathic arthritis (JIA). Anti-CCP antibodies were checked by ELISA in 68 children with JIA, 38 males and 30 females with mean age of 10.6 (+/-4.02) years and...
journal_title:Immunological investigations
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doi:10.1080/08820130802438057
更新日期:2008-01-01 00:00:00
abstract::The transferrin receptor (TR), the HLA DR antigen (DR), and the antigen binding OKT10 (T10) are present on activated lymphocyte populations. The authors have studied their expression and that of antigens defined by eight commercial monoclonal antibodies on peripheral blood lymphocytes of 50 healthy hospital workers ag...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709087089
更新日期:1987-06-01 00:00:00
abstract::The induction of immune tolerance is the most common consequence of protein feeding, i.e., "oral tolerance". In this study we investigated the genetic basis of oral tolerance using various kinds of recombinant and congenic mice, and the cells involved in the development of this phenomenon in mice. The footpad swelling...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139309063423
更新日期:1993-08-01 00:00:00
abstract::Studies demonstrated that deficiency in 17β-estradiol (E2) in postmenopausal women influences their immune system. However, few studies have reported alterations in immunologic presentation during nonnatural menopause in young females. Here we compared the differences in immune response between young C57BL/6N mice wit...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1267203
更新日期:2017-04-01 00:00:00
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journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2012.658940
更新日期:2012-01-01 00:00:00
abstract::Interleukin (IL)-10 response is associated with mortality in patients with sepsis. IL-10 is primarily produced by monocytes and type 2 T helper (Th2) cells. The aim of this study was to investigate differences in IL-10 production between monocytes and Th2 cells in patients with sepsis. Forty patients with sepsis and 3...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1288237
更新日期:2017-05-01 00:00:00
abstract::Objectives: The aim of this study was to assess the genetic association between vascular endothelial growth factor (VEGF) gene polymorphisms and the risk of pre-eclampsia (PE).Methods: A systematic literature search of several databases (PubMed, Embase, and the China National Knowledge Infrastructure (CNKI)) was condu...
journal_title:Immunological investigations
pub_type: 杂志文章,meta分析
doi:10.1080/08820139.2019.1659812
更新日期:2020-02-01 00:00:00
abstract::Recently, we reported the preparation and antigen-presenting properties of hybridoma B-cell clones obtained after fusing non-secreting, non-antigen presenting Balb/c 653-myeloma cells with non-immune SJL spleen cells. It was found that antigen presentation at the clonal level can be specific or non-specific, depending...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138909045785
更新日期:1989-10-01 00:00:00
abstract::A simple and rapid method of ABH phenotyping baboons has been developed which takes advantage of the fact that blood group antigens are expressed on epithelial cells. Use of readily available monoclonal antibodies and phycoerythrin-conjugated second antibody permits rapid identification of ABH phenotypes directly on b...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709055712
更新日期:1987-03-01 00:00:00
abstract:OBJECTIVE:This study determined whether tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10) polymorphisms are associated with susceptibility to dengue. METHODS:a systematic review with meta-analysis was conducted of the associations between the TNF-α (-308G/A) and IL-10 (-819C/T) polymorphisms and dengue. ...
journal_title:Immunological investigations
pub_type: 杂志文章,meta分析
doi:10.1080/08820139.2016.1248560
更新日期:2017-02-01 00:00:00
abstract::Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically s...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109082633
更新日期:1991-08-01 00:00:00
abstract::In this study, we aimed to explore the association among gene variants of five cytokines, tumor necrosis factor alpha (TNF-α), transforming growth factor beta-1 (TGF-β1), interferon gamma (IFN-γ), interleukin-6 (IL-6), and interleukin-10 (IL-10), and clinical parameters and prognosis in patients with multiple myeloma ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1208219
更新日期:2017-01-01 00:00:00
abstract::The purpose of this presentation was to demonstrate that the red blood cell is not a hapless sac of hemoglobin and that much research is still needed for better understanding of its complexities. After a brief historical introduction the following subjects are presented: 1). Phosphofructokinase is the rate limiting st...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139509062760
更新日期:1995-01-01 00:00:00
abstract::The aim of the study was to assess whether genotypes in the Toll-like receptor 4 gene and in the promoter of the interleukin-18 gene are associated with the susceptibility to chronic periodontitis. 108 chronic periodontitis patients and 76 controls were genotyped for c.896A>G/1196C>T (TLR4 gene) and for c.-368G>C/ c.-...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130902846290
更新日期:2009-01-01 00:00:00
abstract::Background: Immunosuppressive agents are necessary to enhance allograft tolerance after transplantation and the treatment of autoimmune disorders. Regulatory T cells (Tregs) play a pivotal role in improving allograft tolerance and determining the fate of transplanted organs. Therefore, the aim of this study was to inv...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1571506
更新日期:2019-07-01 00:00:00
abstract::In this study we demonstrate a differential transcription of H-2K and H-2D class-I genes in two different tumor cell clones; one is highly metastatic (IE-7) and the other is not metastatic (IC-9), both derived from the same fibrosarcoma, T-10, induced in an (H-2b x H-2k)F1 mouse. The expression of the two parental H-2...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109082628
更新日期:1991-08-01 00:00:00
abstract::Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system. Matrix metalloproteinases (MMPs) play an important role in breakdown of blood-brain barrier, transmigration, and invasion of immune cells and formation of MS lesions. The aim of present study was to investigate the influence of MMP-...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1180303
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND:Association studies of genes encoding cytokines that play an important role in inflammatory response represent one approach to finding type 1 diabetes (T1D) disease genes. The aim of this study was to investigate the association of single nucleotide polymorphisms (SNPs) within cytokine genes with T1D in a co...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1416398
更新日期:2018-04-01 00:00:00
abstract::Intravenous immunoglobulins (IVIg) have been used as an immunomodulatory therapy in a variety of diseases. Several mechanisms of action have been proposed, one of which is interference with the cytokine network. We have investigated the effect of IVIg on the cytotoxicity of human TNF alpha. IVIg was capable of protect...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139709088541
更新日期:1997-08-01 00:00:00
abstract::Previous work had implicated T cells within the stimulating population as having a significant role in the MLR. We now report that depletion of T cells from the stimulators caused a reduction in proliferative response in mixed leukocyte reaction (MLR) by 50% or more. This response could be restored by adding T-enriche...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138609042020
更新日期:1986-03-01 00:00:00
abstract::Although the T cell costimulatory molecules CD2 and CD28 are enriched within the immunological synapse (IS), it has been suggested that costimulatory molecules need not be localized to the contact site between a T cell and an antigen-presenting cell (APC) in order to costimulate T cell activation. To determine whether...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701555035
更新日期:2008-01-01 00:00:00
abstract::Thrombocytopenia as a complicating event in human immunodeficiency infection is a rather common hematological disorder and in many aspects resembles idiopathic(immune) thrombocytopenic purpura (ITP). Consequently, treatment of severe cases is similar to treating ITP, but steroids should be administered with particular...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139109050791
更新日期:1991-04-01 00:00:00
abstract::Mice were immunized with 14 free (i.e. not conjugated to any carrier) synthetic peptides representing the entire human hemoglobin alpha-chain. Antibodies against each peptide were determined using solid phase radioimmunoassay, both with free peptides and peptides coupled to a protein carrier as the coating antigen. It...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138909050764
更新日期:1989-08-01 00:00:00
abstract::Patients with metastatic breast carcinoma (BCa) have defective peripheral blood lymphocyte (PBL) responsiveness to mitogenic and antigenic stimulation. In the following experiments, glycopeptides were isolated from the pronase digests of breast carcinoma tissue. After elution from Sephadex G-50, glycopeptides from BCa...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809055715
更新日期:1988-03-01 00:00:00
abstract::There is a continuing need for alternatives to current human adjuvants. Recombinant protein vaccines, which target antigen to human Fc gamma receptor type I (hFcgammaRI) on hFcgammaRI-expressing antigen presenting cells, provide one potential alternative. Using a recombinant anti-hFcgammaRI-antigen fusion protein and ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130500265372
更新日期:2005-01-01 00:00:00