Abstract:
:Recently, we reported the preparation and antigen-presenting properties of hybridoma B-cell clones obtained after fusing non-secreting, non-antigen presenting Balb/c 653-myeloma cells with non-immune SJL spleen cells. It was found that antigen presentation at the clonal level can be specific or non-specific, depending on the particular B-cell clone. In the present work, one specific and one general presenter B-cell clones were tested for their epitope presentation ability to SJL T-cells that were specific to lysozyme or myoglobin. B-cell clone A1G12, a general presenter which presented both lysozyme and myoglobin to their respective T-cell lines, was found to present all five myoglobin epitopes while clone A1L16, a lysozyme specific presenter presented only one of the three epitopes of lysozyme. The latter reveals a hitherto unknown submolecular specificity (to a given epitope within a protein) for antigen presenting cells at the clonal level. Therefore, the specificity of T-cell recognition does not only derive from the T-cell but may also be dependent on the epitope specificity of the antigen-presenting B-cell.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
Cohly HH,Morrison DR,Atassi MZdoi
10.3109/08820138909045785subject
Has Abstractpub_date
1989-10-01 00:00:00pages
987-92issue
8eissn
0882-0139issn
1532-4311journal_volume
18pub_type
杂志文章abstract::Functional defects of polymorphonuclear leukocytes can lead to clinical immunodeficiency. Understanding these conditions requires a knowledge of normal polymorph physiology and the means to analyse various aspects of polymorph function. Primary diseases of polymorph function are rare, whereas disorders secondary to ot...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820138809055721
更新日期:1988-04-01 00:00:00
abstract::Plasmodium falciparum CTL epitope minigenes containing HLA-A2 and HLA-B7 subtype supermotifs were cloned into a plasmid expression vector; this expression was measured in eight human HLA class I molecule specific cell lines. Three assays for in vitro antigen presentation analysis were developed to examine the cross-bi...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120019206
更新日期:2003-02-01 00:00:00
abstract:BACKGROUND:Association studies of genes encoding cytokines that play an important role in inflammatory response represent one approach to finding type 1 diabetes (T1D) disease genes. The aim of this study was to investigate the association of single nucleotide polymorphisms (SNPs) within cytokine genes with T1D in a co...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1416398
更新日期:2018-04-01 00:00:00
abstract::VH4-34 gene encoded autoantibodies are elevated in systemic lupus erythematosus (SLE) and in other diseases associated with B-cell hyperproliferation/dysfunction. One of the autoantigens recognized by VH4-34-encoded antibodies are branched/linear poly N-acetyl lactosamine chains. Since the anti-carbohydrate response i...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2015.1015682
更新日期:2015-01-01 00:00:00
abstract::The effect of two Chinese traditional drugs, Dang Gui injection prepared from Angelica sinensis and C 21 Ester glucoside (GB) extracted from Cynanchus auriculatus on in vitro production of IL-2 has been studied. The IL-2 was produced by Con A stimulation of mouse spleen mononuclear cells. The IL-2 activity was assayed...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709030565
更新日期:1987-04-01 00:00:00
abstract::Identification and characterization of antigenic proteins of Setaria cervi (bovine filarial parasite) adults and microfilariae was done by immunoblotting technique using hyperimmune rabbit sera against S. cervi and Brugia malayi. The antigens recognized by these sera were detected by using 125I protein-A followed by a...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709030571
更新日期:1987-04-01 00:00:00
abstract::Immunological parameters previously shown to constitute an increased risk for progression towards AIDS have been observed in clinically asymptomatic individuals considered to be at risk for this syndrome. These parameters include severely decreased numbers of T helper cells (count below 400/mm3 blood for CD4+ cells we...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809055726
更新日期:1988-04-01 00:00:00
abstract::Flow cytometric analyses of immune cell proliferation, differentiation, and function are limited by the number of different fluorochromes that can be resolved simultaneously. Additional colors to expand functional analytic capability will facilitate higher dimensional analyses of heterogeneous cell populations by basi...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701712461
更新日期:2007-01-01 00:00:00
abstract::A simple and rapid method of ABH phenotyping baboons has been developed which takes advantage of the fact that blood group antigens are expressed on epithelial cells. Use of readily available monoclonal antibodies and phycoerythrin-conjugated second antibody permits rapid identification of ABH phenotypes directly on b...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709055712
更新日期:1987-03-01 00:00:00
abstract::The occurrence of apoptosis in cultured blood cells from HIV+ patients is well-documented. However, the relationship of this process to cytokine production is still undefined. We measured the production of TNF-beta by mitogen-stimulated PBLs from 33 HIV+ patients, while simultaneously assessing their development of ap...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139609059311
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:Changes in cytokine expression have been frequently found in patients with inflammatory bowel disease (IBD). Cytokine values outside the normal range may be somewhat related to common polymorphisms within cytokine genes. OBJECTIVE:The present study was designed to investigate the possible association betwee...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1360343
更新日期:2017-10-01 00:00:00
abstract::Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system. Matrix metalloproteinases (MMPs) play an important role in breakdown of blood-brain barrier, transmigration, and invasion of immune cells and formation of MS lesions. The aim of present study was to investigate the influence of MMP-...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1180303
更新日期:2016-08-01 00:00:00
abstract::Studies presented examine the origin of IgA plasma cells in B lineage chimeric mice constructed by reconstituting lethally irradiated mice with a mixture of syngeneic bone marrow cells and peritoneal cells from Ig heavy chain allotype congenic donors. In these mice, essentially all B cells in spleen and Peyer's patche...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138909112226
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:Infections are usually involved in the pathogenesis of spondyloarthritis (SpA). Mannose-binding lectin (MBL) is a component of the innate immune system with an important role in microbial defense. OBJECTIVE:To study the prevalence of MBL deficiency in SpA patients as well as its influence in the clinical pr...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1237525
更新日期:2017-02-01 00:00:00
abstract::Champedak (Artocarpus integer) lectin-M is a lectin with high specificity and affinity for the core-mannosyl residues of the N-linked oligosaccharides of glycoproteins. We have studied the interaction of the champedak seed lectin with human serum glycoproteins that were resolved by 2-dimensional (2-D) gel electrophore...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-100104021
更新日期:2001-05-01 00:00:00
abstract::The lectin from Amaranthus leucocarpus (ALL) is specific for N-acetyl-D-galactosamine and inhibits phagocytosis of Leishmania mexicana promastigotes in Balb/c mice peritoneal macrophages by 38%. The lipophosphoglycan (LPG) purified from L. mexicana inhibits penetration of promastigotes into peritoneal macrophages by 3...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract:BACKGROUND:The pathological mechanisms associated with the occurrence and development of Behcet's disease (BD) are not yet known. Two large genome-wide association surveys revealed an association between interleukin (IL)-23R single nucleotide polymorphism and BD. This study aimed to investigate the association between ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1698600
更新日期:2020-08-01 00:00:00
abstract::Although the T cell costimulatory molecules CD2 and CD28 are enriched within the immunological synapse (IS), it has been suggested that costimulatory molecules need not be localized to the contact site between a T cell and an antigen-presenting cell (APC) in order to costimulate T cell activation. To determine whether...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130701555035
更新日期:2008-01-01 00:00:00
abstract::The contribution of interleukin-2 (IL-2)-responsive bystander cells to the proliferative responses of human peripheral blood T lymphocytes to antigens used for sensitization such as Purified Protein Derivative (PPD), Tetanus toxoid (T T) and Influenza virus was investigated. Marked proliferation of the unfractionated ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809055720
更新日期:1988-03-01 00:00:00
abstract::A well-characterized recombinant anti-idiotype to an anti-DNA antibody can be useful for studies of the regulation of anti-DNA-producing B cells. Using a hybridoma technique, a monoclonal anti-idiotypic antibody, designated O2F3, was obtained, and its scFv gene was constructed. O2F3 single chain Fv (scFv) was produced...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120016241
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND:IL-23/Th17 signaling pathway plays a crucial role in the cell-mediated immune response against bacterial infections and also in the pathogenesis of inflammatory and autoimmune diseases. Recent studies indicate that Th17 cell-associated cytokines are involved in the progression and maintenance of valvular les...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2018.1493053
更新日期:2018-10-01 00:00:00
abstract::Monocyte chemoattractant protein 1 (MCP-1) plays an important role in the development of periodontitis. The purpose of this investigation was to evaluate association of the MCP-1 gene polymorphism with chronic periodontitis in patients with end-stage renal disease (ESRD). One hundred fifty ESRD patients with chronic ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1702052
更新日期:2020-11-01 00:00:00
abstract::Genome-wide association studies have led to the discovery of several susceptibility genes related to autoimmune thyroid diseases (AITDs). However, controversial results have been reported regarding the role of single-nucleotide polymorphism (SNP) of CD40 in the disease susceptibility. The objective of this study was t...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1319382
更新日期:2017-08-01 00:00:00
abstract::We investigated the functional and subset surface marker characteristics of supramammary lymph node T cell populations at sites proximal and distal to the mammary region of goats repeatedly injected with heat-treated Staphylococcus aureus antigen (HK-SAC). Flow cytometric studies showed quantitative differences in CD4...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139209066184
更新日期:1992-04-01 00:00:00
abstract::We report a method combining mixed lymphocyte reaction (MLR) using a carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeling technique, intracellular cytokine immunofluorescence staining (ICIS), and multiparameter flow cytometry for simultaneous determination of proliferation and cytokine-secreting activity in...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120038079
更新日期:2004-08-01 00:00:00
abstract::Previous work had implicated T cells within the stimulating population as having a significant role in the MLR. We now report that depletion of T cells from the stimulators caused a reduction in proliferative response in mixed leukocyte reaction (MLR) by 50% or more. This response could be restored by adding T-enriche...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138609042020
更新日期:1986-03-01 00:00:00
abstract:OBJECTIVE:This study aimed to examine the association of three functional IRF5 rs10954213, rs3757385, and rs41298401 polymorphisms with susceptibility to unexplained recurrent pregnancy loss (RPL) among Iranian women from south of Iran. METHODS:176 women with unexplained RPL and 173 healthy postmenopausal controls wer...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1225759
更新日期:2017-01-01 00:00:00
abstract::Neutrophil chemotactic defects have been reported previously in patients with hyper-IgE syndrome. Bi-allelic mutations in dedicator of cytokinesis 8 (DOCK8) gene usually cause an autosomal recessive hyper-IgE syndrome phenotype. Data are lacking about expression of DOCK8 protein in neutrophils or the possible role of ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1567533
更新日期:2019-05-01 00:00:00
abstract::Polycyclic aromatic hydrocarbons (PAHs) are widely distributed and relocated in the environment as a result of the incomplete combustion of organic matter. Many PAHs and their epoxides are highly toxic, mutagenic and/or carcinogenic to microorganisms as well as to higher systems including humans. BP is one of the most...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2015.1043669
更新日期:2015-01-01 00:00:00
abstract::As a result of strong experimental data supporting effectiveness and safety, herb-based immunomodulators are paving way as alternative sources of potent adjuvants for vaccines. In this study, the immunostimulatory and adjuvant properties of AcF1, a flavonoids-rich fraction of Alchornea cordifolia extract, was evaluate...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820130903496793
更新日期:2010-01-01 00:00:00