Abstract:
:We examined the blocking ability of poly(vinyl alcohol) (PVA) in enzyme immunoassays by coating polystyrene microtiter wells with PVA of different molecular weights (MW) and percent hydrolysis (%Hyd). Blocking ability was measured by the differences in non-specific binding of an anti-rabbit IgG-horseradish peroxidase conjugate to coated and uncoated wells. PVA with a MW of 124,000-186,000 and > 99 %Hyd was the most effective in suppressing the binding of the conjugate. This PVA at 0.5% (w/v) was significantly better at reducing non-specific binding than commonly used blocking agents and did not interfere with the specific binding of the conjugate to antigen-coated microtiter wells.
journal_name
Immunol Investjournal_title
Immunological investigationsauthors
Rodda DJ,Yamazaki Hdoi
10.3109/08820139409066836subject
Has Abstractpub_date
1994-11-01 00:00:00pages
421-8issue
6-7eissn
0882-0139issn
1532-4311journal_volume
23pub_type
杂志文章abstract::Antisera to the chemically synthesized trisaccharide, Gal beta 1-3(GlcNAc beta 1-6)GalNAc, and disaccharide, GlcNAc beta 1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically s...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109082633
更新日期:1991-08-01 00:00:00
abstract::In this study we examined a panel of CD4+ antigen specific/MHC restricted T cell clones for their ability to secrete IL-2, IL-4, and IFN-gamma upon stimulation with con A, three lymphokines which are diagnostic for the TH1 and TH2 subtypes of helper T cells. Eight of the twelve clones we analyzed did not fit the class...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139109054925
更新日期:1991-02-01 00:00:00
abstract::Interleukin-31 (IL-31) has diverse biological functions. Increased IL-31 has been found in some skin and autoimmune diseases. There has been no study reporting the association between IL-31 and primary biliary cholangitis (PBC). This study was designed to determine serum IL-31 level and to explore its diagnostic value...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2020.1785490
更新日期:2020-07-02 00:00:00
abstract::IL-2 production by the phytohemaglutinin (PHA)-stimulated mononuclear cells (MNC) of peripheral blood (PB) and synovial fluid (SF) from patients with rheumatoid arthritis (RA), other arthritic diseases (OAD) and age-matched normal controls were studied, and the activity of IL-2 inhibitor in sera of studied subject was...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139509060720
更新日期:1995-11-01 00:00:00
abstract:OBJECTIVE:To study the relation between CD226 rs763361 gene polymorphism and CD226 serum level and to evaluate their role in susceptibility and disease activity of RA in a cohort of Egyptian individuals. METHODS:The serum level of CD226 was measured using a suitable ELISA kit and the CD226 rs763361 gene polymorphism w...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2018.1423570
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:Rheumatic fever (RF) and its sequel rheumatic heart disease (RHD) is an autoimmune disease caused by an abnormal host immune response to group A streptococcus (GAS) infection. The HLA class II molecules are entailed in immune-mediated infectious, inflammatory, and autoimmune diseases including RHD. However, ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2020.1822864
更新日期:2020-09-23 00:00:00
abstract::CD44 comprises a family of type I transmembrane glycoproteins that is expressed on a wide range of cells including those of epithelial, lymphoid and myeloid lineage. Although expression of CD44 in the small intestine is typically localised in the crypts of Lieberkuhn, we have reported the expression of CD44 on mature,...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820130009060867
更新日期:2000-08-01 00:00:00
abstract::For many years, isolation in tissue culture (TC) was considered the test of choice for diagnosis of Chlamydia trachomatis infection. Non-culture tests, such as direct fluorescent antibody (DFA) and enzyme immunoassay (EIA) which detected chlamydial antigens in clinical specimens, made chlamydia diagnostic tests more w...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139709048923
更新日期:1997-01-01 00:00:00
abstract::Adjuvant interleukin (IL)-2 immunotherapy has been used in the treatment of different malignant dieseases. However, clinical results have been rather disappointing. Therefore, further investigations on IL-2-induced mediators of cytotoxicity seem to be necessary in order to possibly create cytokine cocktails which coul...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1081/imm-120030916
更新日期:2004-05-01 00:00:00
abstract::Studies demonstrated that deficiency in 17β-estradiol (E2) in postmenopausal women influences their immune system. However, few studies have reported alterations in immunologic presentation during nonnatural menopause in young females. Here we compared the differences in immune response between young C57BL/6N mice wit...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1267203
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND:Changes in cytokine expression have been frequently found in patients with inflammatory bowel disease (IBD). Cytokine values outside the normal range may be somewhat related to common polymorphisms within cytokine genes. OBJECTIVE:The present study was designed to investigate the possible association betwee...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1360343
更新日期:2017-10-01 00:00:00
abstract::Jeevaneeya rasayana is an ayurvedic polyherbal formulation, with antirheumatic potential. The present study investigates the therapeutic efficacy of isolated total alkaloid fraction of Jeevaneeya Rasayana (AJR) in treating rheumatoid arthritis in a rat model of Adjuvant-induced arthritis (AIA). Paw swelling, inflammat...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2014.936937
更新日期:2015-01-01 00:00:00
abstract::Myeloid derived suppressor cells (MDSCs) are a heterogeneous population of cells that inhibit anti-tumor immunity through a variety of mechanisms. Malignant gliomas are heavily infiltrated by myeloid cells, some of which appear to share biological functions of MDSCs. Our data with mouse de novo gliomas indicate critic...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.3109/08820139.2012.689591
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Molecular epidemiological studies have sought associations between interleukin-6 (IL-6) polymorphisms and the risk of systemic lupus erythematosus (SLE); however, the results are controversial. Therefore, we conducted a meta-analysis with trial sequential analysis to evaluate a more accurate estimation of th...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2020.1769646
更新日期:2021-02-01 00:00:00
abstract::Objectives: Type 1 diabetes mellitus (T1D) has been disclosed to be associated with an elevated risk of cardiovascular disease (CVD), as well as increased risks of losing bone mass and progression of osteoporosis (OP). Osteoprotegerin (OPG), as a decoy receptor, has been demonstrated to play a critical role in bone me...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1710531
更新日期:2021-02-01 00:00:00
abstract::Results of recent studies indicated that a monoclonal ART18 antibody recognizes the receptor sites or closely related structures for IL-2 on activated rat T-cells. The major histocompatibility complex (MHC) class II (Ia) antigens (RT1B and RT1D in rat), detected with OX6 antibody, and OX17 antibody, may be present on ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138709087087
更新日期:1987-06-01 00:00:00
abstract::Th17 cells of the intestine and colon can produce several important cytokines during mucosal inflammation. However, few studies have focused on the role of IL-26 in intestinal inflammations. Colonic epithelial cells express receptors for IL-26, and this cytokine has been shown to induce the HT-29 colonic epithelial ce...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1594247
更新日期:2019-11-01 00:00:00
abstract::In order to characterize the immune response to BK virus, a human polyomavirus containing dsDNA and host cell histones, we followed the appearance of antibodies in five outbred rabbits after intravenous inoculation with purified infectious BK virus without any adjuvant. The animals were followed for 15 weeks after the...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139009042033
更新日期:1990-04-01 00:00:00
abstract::It has become apparent that extreme environmental conditions of Antarctic continent alters many immune responses. The present study was conducted on 28th Indian Antarctic expeditioners. The investigations were carried out to explore the effect of multiple stresses like isolation, cold and UV exposure on human immunity...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2010.494194
更新日期:2010-01-01 00:00:00
abstract::Vitamin D deficiency and vitamin D receptor (VDR) gene polymorphisms have been reported in autoimmune diseases. However, their role in Behçet's disease (BD) and scleroderma remains inconclusive. Our aim was to evaluate vitamin D receptor (ApaI, TaqI) gene polymorphisms in relation to Behçet's disease and scleroderma i...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2016.1180302
更新日期:2016-08-01 00:00:00
abstract::In the present study, we evaluated two novel technologies, the chemiluminescent immunoassay (CIA) QUANTA Flash on BIO-FLASH (Inova Diagnostics, San Diego, CA, USA) and the addressable laser bead immunoassay (ALBIA) on BioPlex™ 2200 (Bio-Rad, Hercules, CA, USA) for the detection of anti-cardiolipin IgG/IgM (aCL) and an...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2019.1647233
更新日期:2020-02-01 00:00:00
abstract::Prolonged survival of C57BL/6 (B6) mice bearing syngenic EL-4 leukemia cells resulted from immunization with irradiated EL-4 cells on the day of inoculation of live leukemia cells. No prolongation of survival was observed if the irradiated cells were injected 6 or 13 days after live cell inoculation. Protection also w...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138509022676
更新日期:1985-12-01 00:00:00
abstract::In the last 15 years, it has become apparent that ovarian cancer is recognized by the immune system, taking into account that T cell infiltration can be associated with increased overall survival. Several studies indicate that a correct combination of cluster of differentiation 8 and cluster of differentiation 4 T cel...
journal_title:Immunological investigations
pub_type: 杂志文章,评审
doi:10.1080/08820139.2016.1186689
更新日期:2016-11-01 00:00:00
abstract::Polyclonal immune monkey serum raised against schizonts of Plasmodium knowlesi (H-strain) showed the presence of antibodies to lactate dehydrogenase (LDH) of P. knowlesi by immunodot enzyme staining method. The anti-LDH antibodies are most probably directed towards an epitope distinct from the catalytic site as shown ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820138809030584
更新日期:1988-08-01 00:00:00
abstract::Hashimoto's thyroiditis (HT) is a T cell-mediated autoimmune disease. However, little is known about the role of different subsets of natural killer (NK) and natural killer T (NKT) cells at the early stage of the HT process. A total of 45 euthyroid patients with new onset HT and 40 age/gender-matched healthy controls ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2013.854377
更新日期:2014-01-01 00:00:00
abstract::Porphyromonas gingivalis (P.g) is the primary bacterial agent in many forms of chronic periodontitis. Since polymorphonuclear leukocytes (PMNs) are first-line responders to P.g.- induced inflammation, and fibrinogen is important for in vivo PMN in this disease, we have studied the effect of N-formyl-methionyl-leucyl-p...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820130500496811
更新日期:2006-01-01 00:00:00
abstract::This research was undertaken to test the in vitro anti-inflammatory action of 5,7,4'-trimethoxy-4-phenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin produced by Streptomyces aureofaciens CMUAc130. The effects of the two coumarins were investigated on the formation of NO, PGE2, and TNF-alpha and also on inducible nitric...
journal_title:Immunological investigations
pub_type: 杂志文章,收录出版
doi:10.1080/08820130600992115
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Natural killer (NK) cells are the potential modulators of inflammatory reactions that exert several unique biological effects and could lead to future adverse events of coronary artery disease (CAD). HYPOTHESIS:The purpose of this study was to find out the possible association of modulation in NK cell, TNK ...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.1080/08820139.2017.1306866
更新日期:2017-07-01 00:00:00
abstract::We investigated the functional and subset surface marker characteristics of supramammary lymph node T cell populations at sites proximal and distal to the mammary region of goats repeatedly injected with heat-treated Staphylococcus aureus antigen (HK-SAC). Flow cytometric studies showed quantitative differences in CD4...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139209066184
更新日期:1992-04-01 00:00:00
abstract::Uric acid can be generated in the gastrointestinal (GI) tract from the breakdown of nucleotides ingested in the diet or from purines released from host cells as a result of pathogen-induced cell damage. Xanthine oxidase (XO) is the enzyme that converts hypoxanthine or xanthine into uric acid, a reaction that also gene...
journal_title:Immunological investigations
pub_type: 杂志文章
doi:10.3109/08820139.2013.864667
更新日期:2014-01-01 00:00:00