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Synthesis of nuclear lamins in BHK-21 cells synchronized with aphidicolin.

Abstract：

:Lamins A, B and C are the major proteins of mammalian nuclear lamina and have been well studied in BHK-21 cells. By synchronizing BHK-21 cells with aphidicolin, a potent inhibitor of DNA alpha-polymerase, we were able to detect a differential pattern of synthesis for nuclear lamins during the cell cycle. Lamin B starts to be synthesized only in S phase up to mitosis while synthesis of lamins A and C remain stable throughout the cell cycle. The precursor of lamin A see its half-life increase from a reported 63 min in interphase cells to 103 min in G2/M cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Foisy S,Bibor-Hardy V

doi

10.1016/s0006-291x(88)80825-8

subject

Has Abstract

pub_date

1988-10-14 00:00:00

pages

205-10

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(88)80825-8

journal_volume

156

pub_type

杂志文章

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