Molecular dosimetry of alkylating agents: quantitative comparison of genetic effects on the basis of DNA adduct formation.

abstract：:The objective of the present study was to develop a cellular phenotype assay for nucleotide excision repair (NER), using benzo[a]pyrene diol epoxide (BPDE) as model mutagen. Since in vitro exposure to BPDE may lead to DNA strand breaks resulting from both direct interaction with DNA and incisions introduced by the rep...

journal_title：Mutagenesis

authors： Vande Loock K,Decordier I,Ciardelli R,Haumont D,Kirsch-Volders M

更新日期：2010-01-01 00:00:00

abstract：:Ionising radiation induces several isolated and clustered DNA lesions in human cells. Depending on the type of lesions, DNA repair pathways get activated to maintain the integrity of the genome. Base excision repair (BER) pathway is known to repair single-strand breaks and base damages through short- and long-patch ge...

journal_title：Mutagenesis

authors： Toprani SM,Das B

更新日期：2015-03-01 00:00:00

abstract：:The effects of salts of 27 elements on recombination and on the production of disomic and/or diploid spores during meiosis of Saccharomyces cerevisiae has been investigated. Be(NO3)2, MgSO4, FeSO4, CuSO4, AgNO3, Na2HAsO4 were inactive on the events studied during meiotic cell division. AuCl4, CdCl2, C4H6O4Pb, SnCl2, K...

journal_title：Mutagenesis

authors： Sora S,Agostoni Carbone ML,Pacciarini M,Magni GE

更新日期：1986-01-01 00:00:00

abstract：:Cell transformation assays (CTAs) have long been proposed as in vitro methods for the identification of potential chemical carcinogens. Despite showing good correlation with rodent bioassay data, concerns over the subjective nature of using morphological criteria for identifying transformed cells and a lack of underst...

journal_title：Mutagenesis

authors： Creton S,Aardema MJ,Carmichael PL,Harvey JS,Martin FL,Newbold RF,O'Donovan MR,Pant K,Poth A,Sakai A,Sasaki K,Scott AD,Schechtman LM,Shen RR,Tanaka N,Yasaei H

更新日期：2012-01-01 00:00:00

abstract：:Alzheimer's disease (AD) is a neurodegenerative disorder of the elderly with a complex etiology due to the interaction between genetic and environmental factors. At least 15% of cases are inherited as an autosomal dominant mutation, but the majority are sporadic. We evaluated cytogenetic alterations, both spontaneous ...

journal_title：Mutagenesis

authors： Trippi F,Botto N,Scarpato R,Petrozzi L,Bonuccelli U,Latorraca S,Sorbi S,Migliore L

更新日期：2001-07-01 00:00:00

abstract：:Fanconi anaemia (FA) patients show cellular sensitivity to a variety of clastogens and prominently to cross-linking agents. Although there is a long-standing clinical impression of radiosensitivity, in vitro studies have yielded conflicting results. In this study, initial radiation-induced DNA damage and kinetics of D...

journal_title：Mutagenesis

authors： Mohseni-Meybodi A,Mozdarani H,Mozdarani S

更新日期：2009-01-01 00:00:00

abstract：:On April 6, 1993, near the town of Tomsk (Russia) there was an accident at the Siberian Chemical Plant (SCP) which resulted in extensive contamination of an area of 250 km(2) to the north of SCP with long-lived radionuclides such as (239)Pu, (137)Cs and (90)Sr. Cytogenetic methods and electron spin resonance (ESR) spe...

journal_title：Mutagenesis

authors： Ilyinskikh NN,Ilyinskikh IN,Porovskiy VA,Natarajan AT,Suskov II,Smirenniy LN,Ilyinskikh EN

更新日期：1999-09-01 00:00:00

abstract：:We report on a new method for the study of radiation-induced chromatid breaks in stimulated human peripheral blood T lymphocytes, involving a combination of a 1-h colcemid block and a short (15 min) calyculin A treatment. We find that this procedure eliminates the problem of centromere splitting when calyculin A is us...

journal_title：Mutagenesis

authors： Shovman O,Riches AC,Adamson D,Bryant PE

更新日期：2008-07-01 00:00:00

abstract：:Carbaryl causes depolymerization of spindle microtubules and apparent uncoupling of karyokinesis and cytokinesis in mitotic V79 cells. The metabolite 1-naphthol has virtually identical effects at equimolar concentrations. The closely related 2-naphthol causes similar configurations, but at a much lower frequency than ...

journal_title：Mutagenesis

authors： Renglin A,Olsson A,Wachtmeister CA,Onfelt A

更新日期：1998-07-01 00:00:00

abstract：:The use of DNA adduct analysis has previously focused on the use of marine organisms for biomonitoring, whereas similar investigations in freshwater organisms are sparse. In that context, we have investigated the relevance of DNA adducts as biomarkers of genotoxicity in the freshwater mussels Dreissena polymorpha. The...

journal_title：Mutagenesis

authors： Châtel A,Faucet-Marquis V,Perret M,Gourlay-Francé C,Uher E,Pfohl-Leszkowicz A,Vincent-Hubert F

更新日期：2012-11-01 00:00:00

abstract：:The micronucleus assay (MN assay) is a well-established assay in genetic toxicology, biomonitoring of mutagen-exposed populations and chromosomal radiosensitivity testing. To evaluate the effect of storage time on the chromosomal radiosensitivity assessment in lymphocytes, micronuclei (MN) yields in blood samples rece...

journal_title：Mutagenesis

authors： Baeyens A,Herd O,Francies FZ,Cairns A,Katzman G,Murdoch M,Padiachy D,Morford M,Vral A,Slabbert JP

更新日期：2016-03-01 00:00:00

abstract：:Basic extracts isolated from lamb meat treated at various temperatures were tested for mutagenicity in Salmonella typhimurium strain TA98 in the presence of S9 mix. Samples of ground lamb patties were cooked for 10 min per side at 100, 150, 200, 250 and 300 degrees C. Low cooking temperatures resulted in products with...

journal_title：Mutagenesis

authors： Sflomos C,Papadopoulou R,Athanasiou K

更新日期：1989-05-01 00:00:00

abstract：:Aneuploidy has important consequences for human health. There is a requirement to detect aneugens that may not be fully satisfied by the current test batter. New cytogenetic techniques look promising. ...

journal_title：Mutagenesis

authors：

更新日期：1998-07-01 00:00:00

abstract：:In order to discriminate between different mechanisms put forward to explain the low observed yields of reciprocal translocation configurations at diakinesis-metaphase I spermatocytes following treatment of mouse stem cell spermatogonia with chemical clastogens, the physiological states of stem cells at the moment of ...

journal_title：Mutagenesis

authors： van Buul PP,Seelen CM,Schöppink K,Goudzwaard JH

更新日期：1992-07-01 00:00:00

abstract：:Five independently isolated glutathione-deficient (gsh-) mutants of Saccharomyces cerevisae with maximally 6% residual glutathione content have been analysed genetically. Complementation as well as tetrad analysis of the homo- and heterozygous diploids constructed by suitable crosses of the five mutants indicated that...

journal_title：Mutagenesis

authors： Kistler M,Maier K,Eckardt-Schupp F

更新日期：1990-01-01 00:00:00

abstract：:Exposure to ambient air particles is associated with elevated levels of DNA strand breaks (SBs) and endonuclease III, formamidopyrimidine DNA glycosylase (FPG) and oxoguanine DNA glycosylase-sensitive sites in cell cultures, animals and humans. In both animals and cell cultures, increases in SB and in oxidatively dama...

journal_title：Mutagenesis

authors： Møller P,Hemmingsen JG,Jensen DM,Danielsen PH,Karottki DG,Jantzen K,Roursgaard M,Cao Y,Kermanizadeh A,Klingberg H,Christophersen DV,Hersoug LG,Loft S

更新日期：2015-01-01 00:00:00

abstract：:We have investigated the relative importance of DNA methylation-directed repair of replication errors to the production of mutation/gene rearrangements in mammalian cells with hypomethylated genomes. Using a series of sibling (Chinese hamster derived) cell clones with stably altered DNA methylation levels, we found th...

journal_title：Mutagenesis

authors： Diver WP,Woodcock DM

更新日期：1989-11-01 00:00:00

abstract：:Alterations of DNA repair enzymes and consequential triggering of aberrant DNA damage response (DDR) pathways are thought to play a pivotal role in genomic instabilities associated with cancer development, and are further thought to be important predictive biomarkers for therapy using the synthetic lethality paradigm....

journal_title：Mutagenesis

authors： Malfatti MC,Antoniali G,Codrich M,Burra S,Mangiapane G,Dalla E,Tell G

更新日期：2020-02-13 00:00:00

abstract：:Swiss albino mice were exposed to formulated cypermethrin (CMR) and/or or chlorpyrifos (CPF) through oral gavages for 60 days. Test doses of CMR (0.69, 1.38 or 2.76mg/kg/day) or CPF (0.5, 1.0 or 2.0mg/kg/day) or CMR + CPF (0.69 + 0.5, 1.38 + 1.0 or 2.76 + 2.0mg/kg/day) were based on the acute oral median lethal doses ...

journal_title：Mutagenesis

authors： Chauhan LK,Varshney M,Pandey V,Sharma P,Verma VK,Kumar P,Goel SK

更新日期：2016-11-01 00:00:00

abstract：:Published data regarding the association between the apurinic/apyrimidinic endonuclease 1 (APE1) T1349G (Asp148Glu) polymorphism and cancer risk show inconclusive results. To derive a more precise estimation of the relationship, we performed a meta-analysis of 27 published studies that included 12 432 cancer cases and...

journal_title：Mutagenesis

authors： Gu D,Wang M,Wang M,Zhang Z,Chen J

更新日期：2009-11-01 00:00:00

abstract：:Non-cytotoxic concentrations (1.5, 3 and 6 microM) of sodium arsenite (SA) were used to study its cytogenetic effects with special reference to the frequency and nature of chromosomal aberrations on the X chromosome at Xq21 and at centromeric regions in CHO9, EM-C11, V79, V-H4 and CHE cell lines using Giemsa and FISH ...

journal_title：Mutagenesis

authors： Radha S,Natarajan AT

更新日期：1998-05-01 00:00:00

abstract：:Polychlorinated biphenyls (PCBs) are stable pollutants, which can be found in almost every compartment of terrestrial and aquatic ecosystems. They are very lipophilic and therefore have the potency of accumulating in the fat stores of animals. The mechanisms by which PCBs exert their adverse effects are still unclear....

journal_title：Mutagenesis

authors： Belpaeme K,Delbeke K,Zhu L,Kirsch-Volders M

更新日期：1996-09-01 00:00:00

abstract：:The old herbal drug aristolochic acid (AA), derived from Aristolochia spp., has been associated with the development of a novel nephropathy, designated aristolochic acid nephropathy (AAN), and urothelial cancer in AAN patients. There is clear evidence that the major components of the plant extract AA, aristolochic aci...

journal_title：Mutagenesis

authors： Arlt VM,Stiborova M,Schmeiser HH

更新日期：2002-07-01 00:00:00

abstract：:A working party was set up by the UK Environmental Mutagen Society to consider alternatives to Aroclor 1254 (Aroclor)-induced S9 in in vitro genotoxicity assays, with the aims of considering whether a replacement for Aroclor in its role in general screening assays could be readily identified. The working party conclud...

journal_title：Mutagenesis

authors： Elliott BM,Combes RD,Elcombe CR,Gatehouse DG,Gibson GG,Mackay JM,Wolf RC

更新日期：1992-05-01 00:00:00

abstract：:A simplified method, using recombinant interleukin-2, foetal bovine serum and freeze-killed feeder cells, has been developed for the mass culture of T-lymphocytes derived from human peripheral blood. In this protocol, bulk cultures can be cryopreserved approximately 8 days after initiation, and subsequent mass culture...

journal_title：Mutagenesis

authors： O'Donovan MR,Freemantle MR,Hull G,Bell DA,Arlett CF,Cole J

更新日期：1995-05-01 00:00:00

abstract：:The over-production of reactive oxygen species (ROS) can cause oxidative damage to a large number of molecules, including DNA, and has been associated with the pathogenesis of several disorders, such as diabetes mellitus (DM), dyslipidemia and periodontitis (PD). We hypothesise that the presence of these diseases coul...

journal_title：Mutagenesis

authors： Corbi SC,Bastos AS,Orrico SR,Secolin R,Dos Santos RA,Takahashi CS,Scarel-Caminaga RM

更新日期：2014-11-01 00:00:00

abstract：:We have conducted a randomized trial which investigated the ability of dietary changes (in particular diets rich in cruciferous vegetables and flavonoids), to increase urinary antimutagenicity and inhibit DNA damage in smokers. Ninety heavy smokers were recruited and randomly assigned to three groups, and were given t...

journal_title：Mutagenesis

authors： Talaska G,Al-Zoughool M,Malaveille C,Fiorini L,Schumann B,Vietas J,Peluso M,Munnia A,Bianchini M,Allegro G,Matullo G,Sacerdote C,Vineis P

更新日期：2006-05-01 00:00:00

abstract：:Blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) is an autosomal dominant disorder characterized by blepharophimosis, ptosis and epicanthus inversus. Based on the presence and absence of premature ovarian failure, two clinical types have been distinguished. Both types of BPES have been mapped to chromosome ...

journal_title：Mutagenesis

authors： Tang S,Wang X,Lin L,Sun Y,Wang Y,Yu H

更新日期：2006-01-01 00:00:00

abstract：:The presence of an adaptive response to low doses of alkylating chemicals in the phototrophic bacteria Rhodobacter sphaeroides and R.capsulatus has been studied. Results obtained show that both strains display this repair response against the challenge doses of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), when they ar...

journal_title：Mutagenesis

authors： Vericat JA,Barbé J

更新日期：1988-03-01 00:00:00

abstract：:With the aim of optimizing the efficiency of S9 fractions used in in vitro mutagenicity assays, different schemes for the induction of liver enzymes in rats were tried and the amount of S9 fraction required was assessed. The activity of 2-anthramine (2AA), 2-acetylaminofluorene (2AAF), 3-methylcholanthrene (3MTCL) and...

journal_title：Mutagenesis

authors： García Franco S,Domínguez G,Pico JC

更新日期：1999-05-01 00:00:00