Abstract:
:With the aim of optimizing the efficiency of S9 fractions used in in vitro mutagenicity assays, different schemes for the induction of liver enzymes in rats were tried and the amount of S9 fraction required was assessed. The activity of 2-anthramine (2AA), 2-acetylaminofluorene (2AAF), 3-methylcholanthrene (3MTCL) and benzo[a]pyrene in bacterial mutagenicity tests was compared with the enzymatic activity in S9 fractions obtained from rats treated with either phenobarbital (NaPB), beta-naphthoflavone (betaNF) or combinations of both. Three pool systems prepared with different amounts of NaPB-induced S9 and betaNF-induced S9 were also analyzed for their activation capacities. Profiles of standard plate incorporation assays with Salmonella typhimurium TA98 increased with the amount of S9 fraction added for all drugs tested, except for 2AA, which showed a maximun of activity at low protein concentrations. According to these profiles, an optimal S9 protein content of 700-1000 microg/plate was estimated. For 2AAF and 3MTCL an S9 fraction obtained following a simultaneous treatment with NaPB (i.p.) and betaNF (oral gavage) (NaPB + betaNF) yielded the greatest response. This preparation was the only one which produced positive activation with 3MTCL as test drug. With the other test drugs all the S9 fractions were very active, including the NAPB + betaNF-induced S9. Both Phase I and Phase II cytochrome P450 enzymatic activities were enhanced in this S9 fraction. These results suggest that the simultaneous treatment (NaPB + betaNF) would be an adequate inducer for in vitro activation when used at 700-1000 microg protein/plate.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
García Franco S,Domínguez G,Pico JCdoi
10.1093/mutage/14.3.323subject
Has Abstractpub_date
1999-05-01 00:00:00pages
323-6issue
3eissn
0267-8357issn
1464-3804journal_volume
14pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::The genotoxicity of alpha-hexachlorocyclohexane (alpha-HCH) was evaluated in primary cultures of mouse, rat and human hepatocytes. DNA fragmentation was measured by the alkaline elution technique and DNA repair synthesis by quantitative autoradiography. A 20 h exposure to subtoxic concentrations ranging from 0.056 to ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.1.79
更新日期:1996-01-01 00:00:00
abstract::Radiation-induced exchange aberrations are thought to arise as a consequence of misrejoining of free ends of DNA double strand breaks (dsbs). In quiescent mammalian cells this process of misrejoining is prevalently taken up by the non-homologous end joining (NHEJ) process. In order to investigate the role of glutathio...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei046
更新日期:2005-09-01 00:00:00
abstract::Basic extracts isolated from lamb meat treated at various temperatures were tested for mutagenicity in Salmonella typhimurium strain TA98 in the presence of S9 mix. Samples of ground lamb patties were cooked for 10 min per side at 100, 150, 200, 250 and 300 degrees C. Low cooking temperatures resulted in products with...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.3.228
更新日期:1989-05-01 00:00:00
abstract::The effects of salts of 27 elements on recombination and on the production of disomic and/or diploid spores during meiosis of Saccharomyces cerevisiae has been investigated. Be(NO3)2, MgSO4, FeSO4, CuSO4, AgNO3, Na2HAsO4 were inactive on the events studied during meiotic cell division. AuCl4, CdCl2, C4H6O4Pb, SnCl2, K...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/1.1.21
更新日期:1986-01-01 00:00:00
abstract::The major risk factor for skin cancer is exposure to UV radiation from sunlight, but other environmental exposures may also play a role in combination with UV. We have studied the effects of combined exposure of primary human skin cells in vitro to UVA, UVB or UVC with benzo[a] pyrene (BaP), an environmental carcinoge...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei042
更新日期:2005-07-01 00:00:00
abstract::Newborns have to cope with hypoxia during delivery and a sudden increase in oxygen at birth. Oxygen will partly be released as reactive oxygen species having the potential to cause damage to DNA and proteins. In utero, increase of most (non)-enzymatic antioxidants occurs during last weeks of gestation, making preterm ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ges022
更新日期:2012-09-01 00:00:00
abstract::Protection from chronic exposure to cosmic radiation, which is primarily composed of protons, in future manned missions to Mars and beyond is considered to be a key unresolved issue. To model the effects of cosmic radiation on a living cell, we used Saccharomyces cerevisiae cells harboring various deletions of DNA rep...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gem055
更新日期:2008-03-01 00:00:00
abstract::There are several reports demonstrating that aneugens may preferentially affect segregation of particular chromosomes in somatic cells. Much less is known on specific susceptibility of individual chromosomes to non-disjunction in mammalian meiosis in response to chemical exposures. To explore possible chromosome-speci...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/16.5.407
更新日期:2001-09-01 00:00:00
abstract::A transgenic mouse strain with a high copy number of rescuable lacZ sequences was evaluated for its effectiveness in detecting lacZ- mutations in selected tissues. Procarbazine, cyclophosphamide, ethylnitrosourea, 7,12-dimethylbenz[a]anthracene (DMBA), acrylamide and chlorambucil were tested following either single or...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.1.7
更新日期:1993-01-01 00:00:00
abstract::Recombinant lambda phage DNA containing segments of human O6-methylguanine-DNA transferase gene was employed to localize this gene among the human chromosomes using non-radioactive in situ hybridization technique. This gene was found to be present at the telomeric end, 26q, of the long arm of the chromosome 10. ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/7.1.83
更新日期:1992-01-01 00:00:00
abstract::Due to the very limited transduction capacity of hitherto available vectors, the success of gene therapy of tumours by means of suicide genes has so far essentially depended on the transfer of toxic drug metabolites from transduced (metabolizing) cells to adjacent non-transduced cells via gap junctions (bystander effe...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geh002
更新日期:2004-01-01 00:00:00
abstract::In this study, we determined the induction and time-dependent accumulation of micronuclei in the peripheral blood of transgenic C57BL/6 p53+/- mice (p53+/- mice), FVB/N Tg.AC v-Ha-ras mice (Tg.AC mice) and their isogenic parental strains, FVB/N and C57BL/6 following inhalation exposure to benzene. Our objective was to...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/16.2.163
更新日期:2001-03-01 00:00:00
abstract::Alcohol drinking-derived acetaldehyde is believed to cross-link DNA and induce sister chromatid exchanges in peripheral blood lymphocytes. However, little population data are available to illustrate effects of alcohol-derived acetaldehyde on DNA migration as assayed by the comet assay in peripheral lymphocytes. In the...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gep008
更新日期:2009-07-01 00:00:00
abstract::Mutagens in the semivolatile phase of airborne particulate matter, diesel and gasoline engine emissions were investigated using chemical and biological assays. The previously unknown mutagens, 1-(3-)nitro-6-azabenzo[a]pyrenes (1-N-6-ABP and 3-N-6-ABP) and 1-(3-)nitro-6-azabenzo[a]pyrene-N-oxides (1-N-6-ABPO and 3-N-6-...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/9.1.47
更新日期:1994-01-01 00:00:00
abstract::Barrett's oesophagus (BO) carries an increased risk of progression to oesophageal adenocarcinoma. Chromoendoscopy with methylene blue (MB) can be used to facilitate identification of BO and target areas for biopsy. If photoexcited, MB can generate reactive oxygen species and genotoxic photodegradation products leading...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gep004
更新日期:2009-05-01 00:00:00
abstract::1,8-Dinitropyrene (1,8-DNP) was clearly mutagenic at the hprt locus in CHO cells, but not detectably mutagenic in V79 cells, following a 3-h treatment period. Preliminary data indicate that CHO, but not V79, cells have measurable levels of N-acetyltransferase activity, and this may contribute to the differential sensi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/5.3.275
更新日期:1990-05-01 00:00:00
abstract::Mutation induction by UV irradiation was studied in a retroviral vector integrated in one copy per cell at various chromosomal positions. As a mutational target, hamster hprt cDNA was present on the retroviral vector. To minimize the influence of repair we used repair-deficient hamster cells, V-H1 and UV5, as a recipi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.5.399
更新日期:1993-09-01 00:00:00
abstract::Colcemid (COM) and econazole (EZ) were tested for induction of mitotic arrest and C-mitotic effects in mouse bone marrow cells and for meiotic delay and induction of hyperploidy in mouse spermatocytes after single injection. Doses of 1 and 3 mg/kg COM were used and bone marrow and spermatocytes were sampled at 2, 6, 1...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.4.409
更新日期:1998-07-01 00:00:00
abstract::Inter-individual differences in susceptibility to breast cancer are partially mediated through the levels of endogenous and exogenous steroid hormones. The CYP17 gene encodes P450c17alpha, an enzyme that is involved in the metabolism of steroid hormones. Increased endogenous steroid hormone levels have been associated...
journal_title:Mutagenesis
pub_type: 杂志文章,meta分析
doi:10.1093/mutage/17.2.119
更新日期:2002-03-01 00:00:00
abstract::BRAF alterations represent a novel indicator of the progression and aggressiveness of thyroid carcinogenesis. So, the main aim of the study was to elucidate the involvement of BRAF gene mutations and its expression in Kashmiri (North India) patients and investigate their association with clinico-pathological character...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/get066
更新日期:2014-03-01 00:00:00
abstract::Escherichia coli K12ND-160 possesses characteristics which make it highly suitable for studies on mutagenic/antimutagenic specificity and has been used to investigate such effects for several agents. Nitrofurazone (NFZ) was used as a mutagen at non-lethal concentrations; it causes mutation from fucose sensitivity to r...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.1.31
更新日期:1989-01-01 00:00:00
abstract::We have characterized six SV40-transformed xeroderma pigmentosum cell lines (XP20S and XP12BE derived from female donors, XP12RO-SV, XP3BR/12SV, XP4PA-SV and XP8CAC-SV from male donors) for their usability in HPRT mutation studies. All cell lines exhibit hypersensitivity, compared with MRC5CV1 cells, towards the cytot...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/12.5.391
更新日期:1997-09-01 00:00:00
abstract::Recent studies suggest increased cancer risk in patients with type 2 diabetes mellitus (T2DM) compared with healthy individuals. The present study aims to assess whether T2DM is associated with increased genome instability and whether a healthy diet with natural foods can improve genome stability in peripheral blood l...
journal_title:Mutagenesis
pub_type: 杂志文章,随机对照试验
doi:10.1093/mutage/ges073
更新日期:2013-03-01 00:00:00
abstract::Cell transformation assays (CTAs) have long been proposed as in vitro methods for the identification of potential chemical carcinogens. Despite showing good correlation with rodent bioassay data, concerns over the subjective nature of using morphological criteria for identifying transformed cells and a lack of underst...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ger053
更新日期:2012-01-01 00:00:00
abstract::Wood dust is recognised as a human carcinogen, based on the strong association of wood dust exposure and the elevated risk of malignant tumours of the nasal cavity and paranasal sinuses [sino-nasal cancer (SNC)]. The study aimed to assess genetic damage in workers exposed to wood dust using biomarkers in both buccal a...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geu003
更新日期:2014-05-01 00:00:00
abstract::On April 6, 1993, near the town of Tomsk (Russia) there was an accident at the Siberian Chemical Plant (SCP) which resulted in extensive contamination of an area of 250 km(2) to the north of SCP with long-lived radionuclides such as (239)Pu, (137)Cs and (90)Sr. Cytogenetic methods and electron spin resonance (ESR) spe...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.5.473
更新日期:1999-09-01 00:00:00
abstract::The principal hydroxy-metabolites of benzene--hydroquinone, catechol and phenol--were assayed in tests for mitotic segregation induction in Aspergillus nidulans diploid strain 19. Hydroquinone was the most effective chemical, increasing the frequency of mitotic segregants up to 10-fold at 1-3 mM. Catechol was similarl...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.3.235
更新日期:1987-05-01 00:00:00
abstract::A modified protocol is described for the in vitro analysis of micronuclei in whole blood or separated lymphocyte cultures. The induction of binucleate cells by various concentrations of cytochalasin B (3, 4.5 or 6 micrograms/ml) was examined at two harvest times (68 or 72 h). An optimal yield was obtained by adding cy...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.4.317
更新日期:1993-07-01 00:00:00
abstract::Aneuploidy has important consequences for human health. There is a requirement to detect aneugens that may not be fully satisfied by the current test batter. New cytogenetic techniques look promising. ...
journal_title:Mutagenesis
pub_type:
doi:10.1093/mutage/13.4.417
更新日期:1998-07-01 00:00:00
abstract::Each of the Escherichia coli tester strains in the WP3101P-WP3106P series contains an F' plasmid with a different base substitution mutation within the lacZ gene. Each of the six possible base substitution mutations, therefore, can be assayed with these strains by Lac(+) reversion. We used the strains to characterize ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/15.4.317
更新日期:2000-07-01 00:00:00