Preterm newborns show slower repair of oxidative damage and paternal smoking associated DNA damage.

abstract：:A transgenic mouse strain with a high copy number of rescuable lacZ sequences was evaluated for its effectiveness in detecting lacZ- mutations in selected tissues. Procarbazine, cyclophosphamide, ethylnitrosourea, 7,12-dimethylbenz[a]anthracene (DMBA), acrylamide and chlorambucil were tested following either single or...

journal_title：Mutagenesis

authors： Hoorn AJ,Custer LL,Myhr BC,Brusick D,Gossen J,Vijg J

更新日期：1993-01-01 00:00:00

abstract：:In the present study we evaluated campthotecin (CPT)-induced chromosomal damage in human lymphocytes in the G0 phase of the cell cycle as revealed by the premature chromosome condensation technique. The results obtained here indicate that CPT was able to induce chromosome fragments in the G0 phase of the cell cycle of...

journal_title：Mutagenesis

authors： Mosesso P,Fonti E,Bassi L,Lorenti Garcia C,Palitti F

更新日期：1999-01-01 00:00:00

abstract：:Previous reports showed the protective effect of the synthetic antioxidant butylated hydroxytoluene (BHT) against the chromosomal damage induced by bleomycin (BLM), cadmium chloride and potassium dichromate. To test the hypothesis that this effect was exerted by inhibition and/or scavenging of reactive oxygen species ...

journal_title：Mutagenesis

authors： Grillo CA,Dulout FN

更新日期：2006-11-01 00:00:00

abstract：:The induction of localized DNA damage within a discrete nuclear volume is an important tool in DNA repair studies. Both charged particle irradiation and laser microirradiation (LMI) systems allow for such a localized damage induction, but the results obtained are difficult to compare, as the delivered laser dose canno...

journal_title：Mutagenesis

authors： Splinter J,Jakob B,Lang M,Yano K,Engelhardt J,Hell SW,Chen DJ,Durante M,Taucher-Scholz G

更新日期：2010-05-01 00:00:00

abstract：:Among the tests used to determine the mutagenic potential of chemicals, the chromosomal aberrations and micronucleus assays play an important role. These tests score either chromosomal structural aberrations at metaphase or micronuclei at interphase. One of the hallmarks of apoptosis is DNA fragmentation into 50-300 k...

journal_title：Mutagenesis

authors： Meintières S,Biola A,Pallardy M,Marzin D

更新日期：2001-05-01 00:00:00

abstract：:Barrett's oesophagus (BO) carries an increased risk of progression to oesophageal adenocarcinoma. Chromoendoscopy with methylene blue (MB) can be used to facilitate identification of BO and target areas for biopsy. If photoexcited, MB can generate reactive oxygen species and genotoxic photodegradation products leading...

journal_title：Mutagenesis

authors： Sturmey RG,Wild CP,Hardie LJ

更新日期：2009-05-01 00:00:00

abstract：:The micronucleus assay (MN assay) is a well-established assay in genetic toxicology, biomonitoring of mutagen-exposed populations and chromosomal radiosensitivity testing. To evaluate the effect of storage time on the chromosomal radiosensitivity assessment in lymphocytes, micronuclei (MN) yields in blood samples rece...

journal_title：Mutagenesis

authors： Baeyens A,Herd O,Francies FZ,Cairns A,Katzman G,Murdoch M,Padiachy D,Morford M,Vral A,Slabbert JP

更新日期：2016-03-01 00:00:00

abstract：:Despite their prime candidate status, polymorphisms near genes involved in DNA repair or in other functions related to genome stability have been conspicuously under-represented in the significant associations reported from genome-wide association studies (GWAS) of cancer susceptibility. In this study, we assessed a s...

journal_title：Mutagenesis

authors： Tomlinson IP,Houlston RS,Montgomery GW,Sieber OM,Dunlop MG

更新日期：2012-03-01 00:00:00

abstract：:An established human hepatoma cell strain (Hep G2) was used in micronuclei (MN) and sister chromatid exchange (SCE) assays to evaluate the clastogenic potential of several indirectly-acting mutagenic carcinogens. Benzo[a]pyrene, cyclophosphamide, dimethyl nitrosamine, hexamethylphosphoramide, pyrene and safrole were s...

journal_title：Mutagenesis

authors： Natarajan AT,Darroudi F

更新日期：1991-09-01 00:00:00

abstract：:Oxidative DNA lesions, constantly generated by both endogenous and environmentally induced reactive oxygen species, are removed via the base excision repair pathway. In bacteria, Fpg and Nei DNA glycosylases, belonging to the helix-two-turn-helix (H2TH) structural superfamily, remove oxidised purines and pyrimidines, ...

journal_title：Mutagenesis

authors： Makasheva KA,Endutkin AV,Zharkov DO

更新日期：2020-02-13 00:00:00

abstract：:Cyclooxygenase 2 (Cox-2, a rate-limiting enzyme in the conversion of arachidonic acid to prostanoids) has been implicated in several physiological and pathological processes, and it has been reported that polymorphisms in the regulatory region of Cox-2 might influence its expression, contributing to the interindividua...

journal_title：Mutagenesis

authors： Tang Z,Nie ZL,Pan Y,Zhang L,Gao L,Zhang Q,Qu L,He B,Song G,Zhang Y,Shukui Wang

更新日期：2011-11-01 00:00:00

abstract：:Activation of the human bladder carcinogen 4-aminobiphenyl (ABP) and its N-hydroxy derivatives was investigated using lysates and subcellular enzyme preparations from cultured human uroepithelial cells (HUC). Mutagenic activation was determined using Salmonella typhimurium strains TA98; TA98/1,8-DNP6, a derivative def...

journal_title：Mutagenesis

authors： Hatcher JF,Rao KP,Swaminathan S

更新日期：1993-03-01 00:00:00

abstract：:An immunohistochemical method for the determination of 6-thioguanine resistant (TGr) lymphocytes in human blood samples has been developed. The new technique is a modification of the autoradiographic assay and uses labelling of DNA synthesizing cells by bromodeoxyuridine (BrdU) instead of tritiated thymidine. The labe...

journal_title：Mutagenesis

authors： Montero R,Norppa H,Autio K,Lindholm C,Ostrosky-Wegman P,Sorsa M

更新日期：1991-03-01 00:00:00

abstract：:Alterations of DNA repair enzymes and consequential triggering of aberrant DNA damage response (DDR) pathways are thought to play a pivotal role in genomic instabilities associated with cancer development, and are further thought to be important predictive biomarkers for therapy using the synthetic lethality paradigm....

journal_title：Mutagenesis

authors： Malfatti MC,Antoniali G,Codrich M,Burra S,Mangiapane G,Dalla E,Tell G

更新日期：2020-02-13 00:00:00

abstract：:To evaluate the genetic damage induced by tuberculosis infection and also by a combination of therapeutic exposure to anti-tuberculosis (anti-TB) drugs (isoniazid + rifampicin + pyrazinamide + ethambutol or streptomycin) we have considered chromosome aberrations (CA) and micronuclei (MN) in binucleate peripheral blood...

journal_title：Mutagenesis

authors： Masjedi MR,Heidary A,Mohammadi F,Velayati AA,Dokouhaki P

更新日期：2000-11-01 00:00:00

abstract：:Isothiocyanates are plant-derived compounds that may be beneficial in the prevention of certain chronic diseases. Yet, by stimulating the production of reactive oxygen species (ROS), isothiocyanates can be genotoxic. Whether antioxidants influence isothiocyanate-induced genotoxicity is unclear, but this situation was ...

journal_title：Mutagenesis

authors： Hoffman JD,Ward WM,Loo G

更新日期：2015-05-01 00:00:00

abstract：:The major risk factor for skin cancer is exposure to UV radiation from sunlight, but other environmental exposures may also play a role in combination with UV. We have studied the effects of combined exposure of primary human skin cells in vitro to UVA, UVB or UVC with benzo[a] pyrene (BaP), an environmental carcinoge...

journal_title：Mutagenesis

authors： Crallan RA,Ingham E,Routledge MN

更新日期：2005-07-01 00:00:00

abstract：:As part of the hazard and risk assessment of chemicals in man, it is important to assess the ability of a chemical to induce mutations in vivo. Because of the commonalities in the molecular initiating event, mutagenicity in vitro can correlate well to the in vivo endpoint for certain compound classes; however, the dif...

journal_title：Mutagenesis

authors： Tennant RE,Guesné SJ,Canipa S,Cayley A,Drewe WC,Honma M,Masumura K,Morita T,Stalford SA,Williams RV

更新日期：2019-03-06 00:00:00

abstract：:This study investigated the effect of glucose and fructose, and advanced glycation end-products (AGEs) on genome damage in WIL2-NS cells, measured using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. The effect of AGEs was investigated using the bovine serum albumin (AGE-BSA) model system induced either w...

journal_title：Mutagenesis

authors： Deo P,McCullough CL,Almond T,Jaunay EL,Donnellan L,Dhillon VS,Fenech M

更新日期：2020-03-27 00:00:00

abstract：:Implementation of the seventh amendment to the EU Cosmetics Directive has driven much research into suitable in vitro alternative assays to support satisfactory risk assessments. One such assay is the reconstructed skin micronucleus (RSMN) assay. First reported in 2006, further development occurred and a standard prot...

journal_title：Mutagenesis

authors： Kidd D,Phillips S,Chirom T,Mason N,Smith R,Saul J,Whitwell J,Clements J

更新日期：2019-12-03 00:00:00

abstract：:Quantitative and qualitative aspects of the in vivo micronucleus-inducing potential of five chemicals were studied using flow cytometric enumeration of micronucleated polychromatic peripheral blood erythrocytes in mice. The chemicals were hydroquinone, vinblastine sulphate, chloral hydrate (tested in two different mou...

journal_title：Mutagenesis

authors： Grawé J,Nüsse M,Adler ID

更新日期：1997-01-01 00:00:00

abstract：:Well-established in vitro methods for testing the genotoxic potency of chemicals--such as the Ames/Salmonella test, the mouse lymphoma assay, the micronucleus test and the chromosomal aberration test--show a high false-positive rate for predicting in vivo genotoxicity and carcinogenicity. Thus, there is a need for mor...

journal_title：Mutagenesis

authors： Mathijs K,Brauers KJ,Jennen DG,Lizarraga D,Kleinjans JC,van Delft JH

更新日期：2010-11-01 00:00:00

abstract：:In the light of the CEC aneuploidy programme eight known or suspected aneugens were evaluated with the human lymphocyte 'in vitro' micronucleus test using the cytochalasin-B technique. Only colchicine, chloral hydrate and hydroquinone induced dose-dependent increases in micronucleus frequencies. The other five chemica...

journal_title：Mutagenesis

authors： Van Hummelen P,Kirsch-Volders M

更新日期：1992-11-01 00:00:00

abstract：:Recent problems with the supply of Difco bacto agar have forced some laboratories to evaluate alternative agars for use in the Salmonella/microsome assay. This led to the independent observation in two laboratories (Boots and Glaxo) that Salmonella typhimurium TA97a is sensitive to certain types of agar that may be us...

journal_title：Mutagenesis

authors： Wilcox P,Wedd DJ,Williams WR,Mee CD,O'Donovan MR

更新日期：1992-01-01 00:00:00

abstract：:The principal hydroxy-metabolites of benzene--hydroquinone, catechol and phenol--were assayed in tests for mitotic segregation induction in Aspergillus nidulans diploid strain 19. Hydroquinone was the most effective chemical, increasing the frequency of mitotic segregants up to 10-fold at 1-3 mM. Catechol was similarl...

journal_title：Mutagenesis

authors： Crebelli R,Conti G,Carere A

更新日期：1987-05-01 00:00:00

abstract：:Non-homologous end joining (NHEJ), the major pathway of double-strand break (DSB) repair in mammalian cells, comprises two subpathways: one that requires the three core factors Ku70/80, DNA-PKcs and XRCC4/LigIV (DNA-PK-dependent NHEJ) and the other that is independent of these factors. Using a cell-free NHEJ assay, we...

journal_title：Mutagenesis

authors： Kuhfittig-Kulle S,Feldmann E,Odersky A,Kuliczkowska A,Goedecke W,Eggert A,Pfeiffer P

更新日期：2007-05-01 00:00:00

abstract：:As part of the third UKEMS collaborative trial, ICI Central Toxicology Laboratory (CTL) tested the three study chemicals ethylmethanesulphonate (EMS), benzo[a]pyrene (B[a]P) and benzidine (BZD) in a microwell adaptation of the L5178Y gene mutation assay using the thymidine kinase (tk) locus. Cofactors for metabolic ac...

journal_title：Mutagenesis

authors： Clay P,Cross MF

更新日期：1990-01-01 00:00:00

abstract：:Deregulation of control of the apoptotic process in Fanconi anaemia (FA) appears to be one of the main features of this disease at the cellular level. We show here that FA cells are resistant to treatments with rhodamine-1,2,3 and doxycycline, which both interfere with mitochondrial functionality by different mechanis...

journal_title：Mutagenesis

authors： Bogliolo M,Borghini S,Abbondandolo A,Degan P

更新日期：2002-01-01 00:00:00

abstract：:Folic acid (FA), the most oxidized and stable form of folate, is commonly used as a dietary supplement and in culture media. FA must be reduced and methylated to become the metabolically active form found in blood and utilized by tissues, i.e. 5-methyltetrahydrofolate (5-MeTHF). 5-MeTHF is the methyl group donor requi...

journal_title：Mutagenesis

authors： Wang X,Fenech M

更新日期：2003-01-01 00:00:00

abstract：:Swiss albino mice were exposed to formulated cypermethrin (CMR) and/or or chlorpyrifos (CPF) through oral gavages for 60 days. Test doses of CMR (0.69, 1.38 or 2.76mg/kg/day) or CPF (0.5, 1.0 or 2.0mg/kg/day) or CMR + CPF (0.69 + 0.5, 1.38 + 1.0 or 2.76 + 2.0mg/kg/day) were based on the acute oral median lethal doses ...

journal_title：Mutagenesis

authors： Chauhan LK,Varshney M,Pandey V,Sharma P,Verma VK,Kumar P,Goel SK

更新日期：2016-11-01 00:00:00