Microwell mutation assays: evaluation of ethylmethanesulphonate, benzo[a]pyrene and benzidine using the tk locus in L5178Y mouse lymphoma cells.

abstract：:Alcohol drinking-derived acetaldehyde is believed to cross-link DNA and induce sister chromatid exchanges in peripheral blood lymphocytes. However, little population data are available to illustrate effects of alcohol-derived acetaldehyde on DNA migration as assayed by the comet assay in peripheral lymphocytes. In the...

journal_title：Mutagenesis

authors： Lu Y,Morimoto K

更新日期：2009-07-01 00:00:00

abstract：:The cytogenetic effects of mimosine, a naturally occurring plant amino acid known to arrest cell-cycle progression at the G1-S border in cultured cells, have been studied. It was found that mimosine inhibits the cell-cycle progression in a dose-dependent manner in primary and transformed Chinese hamster fibroblasts as...

journal_title：Mutagenesis

authors： Jha AN,Hande PM,Mullenders LH,Natarajan AT

更新日期：1995-09-01 00:00:00

abstract：:Over the past several years, we have been evaluating the mutagenicity and clastogenicity of compounds capable of Michael-type reactions. These compounds, including acrylamide, several acrylate and methacrylate esters, vinyl sulfones, and phorone, have been evaluated using TK+/- -3.7.2C mouse lymphoma cells. Mutagenic ...

journal_title：Mutagenesis

authors： Dearfield KL,Harrington-Brock K,Doerr CL,Rabinowitz JR,Moore MM

更新日期：1991-11-01 00:00:00

abstract：:Chromatographic profiles of DNA adducts formed following treatment of Chinese hamster ovary (CHO) cells or C3H10T1/2 mouse embryo fibroblast cells with benzo[a]pyrene (BP), in the presence or absence of rat liver S9 fraction, have been obtained by both direct labelling ([3H]BP) and 32P-post-labelling methods. The prin...

journal_title：Mutagenesis

authors： Watson WP,Smith RJ,Ryan D,Meyer AL

更新日期：1990-07-01 00:00:00

abstract：:1,8-Dinitropyrene (1,8-DNP) was clearly mutagenic at the hprt locus in CHO cells, but not detectably mutagenic in V79 cells, following a 3-h treatment period. Preliminary data indicate that CHO, but not V79, cells have measurable levels of N-acetyltransferase activity, and this may contribute to the differential sensi...

journal_title：Mutagenesis

authors： O'Donovan MR

更新日期：1990-05-01 00:00:00

abstract：:In a sample of healthy subjects (23-59 years old), comprising 49 non-smokers and 23 smokers, we studied the effects of smoking on DNA repair synthesis (UDS) in peripheral lymphocytes. DNA repair after UV-irradiation was measured by [3H]TdR incorporation into DNA (semi-conservative synthesis was inhibited by hydroxyure...

journal_title：Mutagenesis

authors： Celotti L,Furlan D,Ferraro P,Levis AG

更新日期：1989-03-01 00:00:00

abstract：:Non-homologous end joining (NHEJ), the major pathway of double-strand break (DSB) repair in mammalian cells, comprises two subpathways: one that requires the three core factors Ku70/80, DNA-PKcs and XRCC4/LigIV (DNA-PK-dependent NHEJ) and the other that is independent of these factors. Using a cell-free NHEJ assay, we...

journal_title：Mutagenesis

authors： Kuhfittig-Kulle S,Feldmann E,Odersky A,Kuliczkowska A,Goedecke W,Eggert A,Pfeiffer P

更新日期：2007-05-01 00:00:00

abstract：:4-Nitroquinoline 1-oxide (4NQO) is used as a positive control in various genotoxicity assays because of its known mutagenic and carcinogenic properties. The chemical is converted into 4-hydroxyaminoquinoline 1-oxide and gives rise to three main DNA adducts, N-(deoxyguanosin-8-yl)-4AQO, 3-(desoxyguanosin-N (2)-yl)-4AQO...

journal_title：Mutagenesis

authors： Brüsehafer K,Manshian BB,Doherty AT,Zaïr ZM,Johnson GE,Doak SH,Jenkins GJ

更新日期：2016-03-01 00:00:00

abstract：:Macrocyclic polyethers (crown ethers) are a family of compounds which possess the ability to transport ionic species across natural and artificial membranes. Because of this characteristic, they have wide applications in industry and are being investigated for their potential as pharmacologic agents. However, these co...

journal_title：Mutagenesis

authors： Arenaz P,Bitticks L,Pannell K,Garcia S

更新日期：1989-11-01 00:00:00

abstract：:The complementation group (CG) assignment is presented for 74 moderately sensitive (approximately 2-4x sensitivity of parental line based on ratio of D10s) UV-sensitive mutants of Chinese hamster ovary (CHO) cells from the Facility for Automated Experiments in Cell Biology (FAECB) collection. The distribution of mutan...

journal_title：Mutagenesis

authors： Busch D,Greiner C,Rosenfeld KL,Ford R,de Wit J,Hoeijmakers JH,Thompson LH

更新日期：1994-07-01 00:00:00

abstract：:Isothiocyanates are plant-derived compounds that may be beneficial in the prevention of certain chronic diseases. Yet, by stimulating the production of reactive oxygen species (ROS), isothiocyanates can be genotoxic. Whether antioxidants influence isothiocyanate-induced genotoxicity is unclear, but this situation was ...

journal_title：Mutagenesis

authors： Hoffman JD,Ward WM,Loo G

更新日期：2015-05-01 00:00:00

abstract：:In order to discriminate between different mechanisms put forward to explain the low observed yields of reciprocal translocation configurations at diakinesis-metaphase I spermatocytes following treatment of mouse stem cell spermatogonia with chemical clastogens, the physiological states of stem cells at the moment of ...

journal_title：Mutagenesis

authors： van Buul PP,Seelen CM,Schöppink K,Goudzwaard JH

更新日期：1992-07-01 00:00:00

abstract：:3 beta-Hydroxy-13 alpha-amino-13,17-seco-5 alpha-androstan-17-oic-13,17-lactam-p-bis(2-chloroethyl) aminophenoxyacetate (NSC 294859) is a new modified steroidal alkylating agent. This compound was given by i.p. administration to mice bearing different types of tumour. It was found to exhibit good activity in L1210 and...

journal_title：Mutagenesis

authors： Tselepi MR,Demopoulos NA,Catsoulacos P

更新日期：1989-09-01 00:00:00

abstract：:Three short-term assays (the SOS chromotest, the Ames-fluctuation test and the newt micronucleus test) were carried out to evaluate the genotoxicity of four trihalomethanes (chloroform, bromodichloromethane, chlorodibromomethane and bromoform). With the SOS chromotest, all the chemicals studied except chloroform were ...

journal_title：Mutagenesis

authors： Le Curieux F,Gauthier L,Erb F,Marzin D

更新日期：1995-07-01 00:00:00

abstract：:As part of the hazard and risk assessment of chemicals in man, it is important to assess the ability of a chemical to induce mutations in vivo. Because of the commonalities in the molecular initiating event, mutagenicity in vitro can correlate well to the in vivo endpoint for certain compound classes; however, the dif...

journal_title：Mutagenesis

authors： Tennant RE,Guesné SJ,Canipa S,Cayley A,Drewe WC,Honma M,Masumura K,Morita T,Stalford SA,Williams RV

更新日期：2019-03-06 00:00:00

abstract：:The genotoxicity of atenolol, a beta-blocker antihypertensive drug, both in vitro and in vivo, was cytogenetically tested for its ability to induce sister chromatid exchange (SCE) and micronuclei (MN) in cultured peripheral lymphocytes. Also, fluorescence in situ hybridization (FISH) with a centromeric probe was perfo...

journal_title：Mutagenesis

authors： Télez M,Martínez B,Criado B,Lostao CM,Penagarikano O,Ortega B,Flores P,Ortiz-Lastra E,Alonso RM,Jiménez RM,Arrieta I

更新日期：2000-05-01 00:00:00

abstract：:Mutagens in the semivolatile phase of airborne particulate matter, diesel and gasoline engine emissions were investigated using chemical and biological assays. The previously unknown mutagens, 1-(3-)nitro-6-azabenzo[a]pyrenes (1-N-6-ABP and 3-N-6-ABP) and 1-(3-)nitro-6-azabenzo[a]pyrene-N-oxides (1-N-6-ABPO and 3-N-6-...

journal_title：Mutagenesis

authors： Sera N,Fukuhara K,Miyata N,Tokiwa H

更新日期：1994-01-01 00:00:00

abstract：:Genetic damage in workers occupationally exposed to an antineoplastic drug was studied using the micronucleus (MN) test, the comet assay, the hprt gene mutation assay and the TCR gene mutation assay. The subjects were divided into two groups: (i) 21 workers from a plant producing methotrexate (MTX); (ii) 21 controls w...

journal_title：Mutagenesis

authors： Deng H,Zhang M,He J,Wu W,Jin L,Zheng W,Lou J,Wang B

更新日期：2005-09-01 00:00:00

abstract：:The tetrahydrofuran-type abasic site analog (THF) induces large deletion mutations in human cells. To compare the large deletions induced by THF on leading and lagging strand templates, plasmid DNAs bearing the analog at a specific position outside the supF gene were introduced into human U2OS cells. The replicated DN...

journal_title：Mutagenesis

authors： Suzuki T,Katayama Y,Komatsu Y,Kamiya H

更新日期：2019-12-19 00:00:00

abstract：:With the aim of optimizing the efficiency of S9 fractions used in in vitro mutagenicity assays, different schemes for the induction of liver enzymes in rats were tried and the amount of S9 fraction required was assessed. The activity of 2-anthramine (2AA), 2-acetylaminofluorene (2AAF), 3-methylcholanthrene (3MTCL) and...

journal_title：Mutagenesis

authors： García Franco S,Domínguez G,Pico JC

更新日期：1999-05-01 00:00:00

abstract：:The principal hydroxy-metabolites of benzene--hydroquinone, catechol and phenol--were assayed in tests for mitotic segregation induction in Aspergillus nidulans diploid strain 19. Hydroquinone was the most effective chemical, increasing the frequency of mitotic segregants up to 10-fold at 1-3 mM. Catechol was similarl...

journal_title：Mutagenesis

authors： Crebelli R,Conti G,Carere A

更新日期：1987-05-01 00:00:00

abstract：:An established human hepatoma cell strain (Hep G2) was used in micronuclei (MN) and sister chromatid exchange (SCE) assays to evaluate the clastogenic potential of several indirectly-acting mutagenic carcinogens. Benzo[a]pyrene, cyclophosphamide, dimethyl nitrosamine, hexamethylphosphoramide, pyrene and safrole were s...

journal_title：Mutagenesis

authors： Natarajan AT,Darroudi F

更新日期：1991-09-01 00:00:00

abstract：:The XPF and ERCC1 proteins form a tight complex and function as an endonuclease to incise on the 5'-side of pyrimidine dimers in DNA. Levels of both proteins are extremely low in group F xeroderma pigmentosum (XP-F) cells. We transfected XP-F cells with the plasmids expressing XPF or ERCC1 and examined levels of both ...

journal_title：Mutagenesis

authors： Yagi T,Katsuya A,Koyano A,Takebe H

更新日期：1998-11-01 00:00:00

abstract：:A working party was set up by the UK Environmental Mutagen Society to consider alternatives to Aroclor 1254 (Aroclor)-induced S9 in in vitro genotoxicity assays, with the aims of considering whether a replacement for Aroclor in its role in general screening assays could be readily identified. The working party conclud...

journal_title：Mutagenesis

authors： Elliott BM,Combes RD,Elcombe CR,Gatehouse DG,Gibson GG,Mackay JM,Wolf RC

更新日期：1992-05-01 00:00:00

abstract：:The genotoxic effects of benzene in lung cells of mice exposed to single acute doses by inhalation have been estimated by cytogenetic analysis of micronuclei in primary cultures of lung fibroblasts. Mice were nose-only exposed to 1000 p.p.m. for 30 or 60 min or to 3500 p.p.m. for 30 min and sacrificed 24 h after the e...

journal_title：Mutagenesis

authors： Ranaldi R,Bassani B,Villani P,Lombardi CC,Tanzarella C,Pacchierotti F

更新日期：1998-09-01 00:00:00

abstract：:The mechanism responsible for the modification of mutagenicity by chlorophyllin has been investigated using mutagenic compounds with different mechanisms of action, including the monofunctional alkylating agents, N-methyl-N'-nitrosourea (MNU) and ethylmethanesulphonate (EMS); nitrosamines related to tobacco products, ...

journal_title：Mutagenesis

authors： Romert L,Curvall M,Jenssen D

更新日期：1992-09-01 00:00:00

abstract：:There is a correlation between intestinal cancer and diets high in meat, so fried beef, chicken, lamb, pork and fish were tested for their ability to induce mutations in the small intestine of mice. The mice were bred to be heterozygous at the Dlb-1 locus so that loss of the dominant Dlb-1 b allele by mutation could b...

journal_title：Mutagenesis

authors： Heddle JA,Knize MG,Dawod D,Zhang XB

更新日期：2001-03-01 00:00:00

abstract：:Alzheimer's disease (AD) is a neurodegenerative disorder of the elderly with a complex etiology due to the interaction between genetic and environmental factors. At least 15% of cases are inherited as an autosomal dominant mutation, but the majority are sporadic. We evaluated cytogenetic alterations, both spontaneous ...

journal_title：Mutagenesis

authors： Trippi F,Botto N,Scarpato R,Petrozzi L,Bonuccelli U,Latorraca S,Sorbi S,Migliore L

更新日期：2001-07-01 00:00:00

abstract：:It has been hypothesised that positive associations between age and levels of oxidative stress-generated damage to DNA may be related to an age-dependent decline in DNA repair activity. The objective of this study was to investigate the association between age and repair activity of oxidatively damaged DNA in peripher...

journal_title：Mutagenesis

authors： Løhr M,Jensen A,Eriksen L,Grønbæk M,Loft S,Møller P

更新日期：2015-09-01 00:00:00

abstract：:The Mre11 complex (Mre11-Rad50-Nbs1) is involved in a diverse array of DNA metabolic processes including the response to DNA double-strand breaks (DSBs). The structure of Rad50 plays a key role in the DNA-binding and end-bridging activity of the complex. An interesting feature within the central portion of the Rad50 p...

journal_title：Mutagenesis

authors： Cahill D,Carney JP

更新日期：2007-07-01 00:00:00