当前位置： SCI文献检索 > MUTAGENESIS期刊下所有文献 > Investigating genetic damage in workers occupationally exposed to methotrexate using three genetic end-points.

Investigating genetic damage in workers occupationally exposed to methotrexate using three genetic end-points.

Abstract：

:Genetic damage in workers occupationally exposed to an antineoplastic drug was studied using the micronucleus (MN) test, the comet assay, the hprt gene mutation assay and the TCR gene mutation assay. The subjects were divided into two groups: (i) 21 workers from a plant producing methotrexate (MTX); (ii) 21 controls were matched according to age, gender and smoking. Fresh blood samples were collected from the workers and controls. The results of the MN test showed that the mean micronuclei rate (MNR) and mean micronucleated cell rate (MCR) in workers were 10.10 +/- 0.95 per thousand and 8.05 +/- 0.75 per thousand, respectively, which were significantly higher than those (5.48 +/- 0.82 per thousand and 4.38 +/- 0.58 per thousand) in controls (P < 0.01). It was found in the comet assay that the mean tail length (MTL) of workers and controls were 1.30 +/- 0.06 microm and 0.07 +/- 0.01 microm, respectively. There was a significant difference between workers and controls for MTL (P < 0.01), but the difference between the mean tail moment (MTM, 0.23 +/- 0.03) of workers and MTM (0.17 +/- 0.04) of controls was not significant (P > 0.05). The results of hprt gene mutation assay showed that the average mutation frequency (Mf-hprt) of hprt in workers was 1.00 +/- 0.02 per thousand, which was significantly higher than that (0.86 +/- 0.01 per thousand) in controls (P < 0.01). Meanwhile, the results of TCR gene mutation assay indicated that Mfs-TCR gene mutation frequencies of workers and controls were 6.87 +/- 0.52 x 10(-4) and 1.67 +/- 0.14 x 10(-4), respectively, which were significantly different (P < 0.01). The results of our experiment suggest that genetic damage is detectable in the 21 workers occupationally exposed to methotrexate.

journal_name

Mutagenesis

journal_title

Mutagenesis

authors

Deng H,Zhang M,He J,Wu W,Jin L,Zheng W,Lou J,Wang B

doi

10.1093/mutage/gei048

subject

Has Abstract

pub_date

2005-09-01 00:00:00

pages

351-7

issue

5

eissn

0267-8357

issn

1464-3804

pii

gei048

journal_volume

20

pub_type

杂志文章

相关文献

MUTAGENESIS文献大全
  • The Cox-2 -1195 G > A polymorphism and cancer risk: a meta-analysis of 25 case-control studies.

    abstract：:Cyclooxygenase 2 (Cox-2, a rate-limiting enzyme in the conversion of arachidonic acid to prostanoids) has been implicated in several physiological and pathological processes, and it has been reported that polymorphisms in the regulatory region of Cox-2 might influence its expression, contributing to the interindividua...

    journal_title：Mutagenesis

    pub_type： 杂志文章,meta分析

    doi：10.1093/mutage/ger040

    authors： Tang Z,Nie ZL,Pan Y,Zhang L,Gao L,Zhang Q,Qu L,He B,Song G,Zhang Y,Shukui Wang

    更新日期：2011-11-01 00:00:00

  • Preterm newborns show slower repair of oxidative damage and paternal smoking associated DNA damage.

    abstract：:Newborns have to cope with hypoxia during delivery and a sudden increase in oxygen at birth. Oxygen will partly be released as reactive oxygen species having the potential to cause damage to DNA and proteins. In utero, increase of most (non)-enzymatic antioxidants occurs during last weeks of gestation, making preterm ...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/ges022

    authors： Vande Loock K,Ciardelli R,Decordier I,Plas G,Haumont D,Kirsch-Volders M

    更新日期：2012-09-01 00:00:00

  • Detection of nitro-azabenzo[a]pyrene derivatives in the semivolatile phase originating from airborne particulate matter, diesel and gasoline vehicles.

    abstract：:Mutagens in the semivolatile phase of airborne particulate matter, diesel and gasoline engine emissions were investigated using chemical and biological assays. The previously unknown mutagens, 1-(3-)nitro-6-azabenzo[a]pyrenes (1-N-6-ABP and 3-N-6-ABP) and 1-(3-)nitro-6-azabenzo[a]pyrene-N-oxides (1-N-6-ABPO and 3-N-6-...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/9.1.47

    authors： Sera N,Fukuhara K,Miyata N,Tokiwa H

    更新日期：1994-01-01 00:00:00

  • DNA damage and DNA damage response in human bronchial epithelial BEAS-2B cells following exposure to 2-nitrobenzanthrone and 3-nitrobenzanthrone: role in apoptosis.

    abstract：:Nitro-polycyclic aromatic hydrocarbons (nitro-PAHs) are mutagenic and carcinogenic environmental pollutants found in diesel exhaust and on urban air pollution particles. In the present study, human bronchial epithelial BEAS-2B cells were exposed to 2-nitrobenzanthrone (2-NBA) and 3-nitrobenzanthrone (3-NBA). DNA damag...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/ger035

    authors： Oya E,Ovrevik J,Arlt VM,Nagy E,Phillips DH,Holme JA

    更新日期：2011-11-01 00:00:00

  • Urinary and serum mutagenicity studies with rats bearing experimental tumours.

    abstract：:Urine and serum samples of rats bearing three different experimental tumours (hepatocellular carcinoma, myelomonocytic leukemia and mesoblastic nephroma) were investigated for mutagenicity with the Ames Salmonella test. Enhancement of mutagenic activity in TA98 and TA100 was observed only in the case of urine samples ...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/10.1.43

    authors： Varga C,Pocsai Z,Kertai P

    更新日期：1995-01-01 00:00:00

  • The application of comparative genomic hybridization and fluorescence in situ hybridization to the characterization of genotoxicity screening tester strains AHH-1 and MCL-5.

    abstract：:AHH-1 TK+/- is a human B cell-derived lymphoblastoid cell line that constitutively expresses a high level of the cytochrome CYP1A1. The MCL-5 cell line was developed by transfection of AHH-1 with cDNAs encoding the human cytochrome P450s, CYP1A2, CYP2A6, CYP2E1, CYP3A4 and microsomal epoxide hydrolase carried in plasm...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/14.4.417

    authors： Corso C,Parry EM

    更新日期：1999-07-01 00:00:00

  • In vitro genotoxicity testing using the micronucleus assay in cell lines, human lymphocytes and 3D human skin models.

    abstract：:The toxicological relevance of the micronucleus (MN) test is well defined: it is a multi-target genotoxic endpoint, assessing not only clastogenic and aneugenic events but also some epigenetic effects, which is simple to score, accurate, applicable in different cell types. In addition, it is predictive for cancer, ame...

    journal_title：Mutagenesis

    pub_type： 杂志文章,评审

    doi：10.1093/mutage/geq068

    authors： Kirsch-Volders M,Decordier I,Elhajouji A,Plas G,Aardema MJ,Fenech M

    更新日期：2011-01-01 00:00:00

  • Enhanced synergism between caffeine and mitomycin C in the induction of cytogenetic aberrations in thymidine kinase-deficient Friend murine erythroleukaemia cells.

    abstract：:The thymidine kinase-deficient subclone, 707BUF, of the Friend murine leukaemia cell line exhibits increased sensitivity to the induction of cytogenetic aberrations by mitomycin C (MMC) relative to wild-type clone 707. It has been suggested that thymidine kinase-deficient cells may be highly mutagen-sensitive through ...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/1.3.173

    authors： McKelvey VJ,McKenna PG

    更新日期：1986-05-01 00:00:00

  • The hOGG1 Ser326Cys polymorphism contributes to cancer susceptibility: evidence from 83 case-control studies.

    abstract：:The Ser326Cys polymorphism in the human 8-oxogunaine DNA glycosylase (hOGG1) gene had been implicated in cancer susceptibility. Studies investigating the associations between the Ser326Cys polymorphism and cancer susceptibility showed conflicting results. To derive a more precise estimation of the relationship, a meta...

    journal_title：Mutagenesis

    pub_type： 杂志文章,meta分析

    doi：10.1093/mutage/ger083

    authors： Wang W,Wang M,Chen Y,Zhang Z,Wang S,Xu M,Wang B,Zhao Q,Zhang Z

    更新日期：2012-05-01 00:00:00

  • Inter-individual variation in the mutagenic activation of 2-acetylaminofluorene by human liver in relation to animal metabolic models.

    abstract：:A relatively large, reproducible inter-individual variation was found in the ability of 17 human liver S9 samples to mediate the mutagenicity of 2-acetylaminofluorene (2AAF) in Salmonella typhimurium strain TA98. In an animal model, variation in metabolic activation of 2AAF did not appear to relate to the phenotype of...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/3.4.323

    authors： Smith AJ,Chipman JK

    更新日期：1988-07-01 00:00:00

  • Biomonitoring of four European populations occupationally exposed to pesticides: use of micronuclei as biomarkers.

    abstract：:This paper presents the results obtained within the framework of an EU research project aimed at investigating the relationship between occupational exposure to pesticides and the induction of cytogenetic damage. Populations from Greece, Spain, Poland and Hungary, all of them characterised by intensive agricultural ac...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/18.3.249

    authors： Pastor S,Creus A,Parrón T,Cebulska-Wasilewska A,Siffel C,Piperakis S,Marcos R

    更新日期：2003-05-01 00:00:00

  • Direct and indirect non-disjunction in the origin of trisomy in cultured human lymphocytes.

    abstract：:The aim of the present work was to investigate the processes involved in the origin of trisomic karyotypes, i.e. co-migration of sister chromatids (mitotic non-disjunction, MND) and recovery of micronuclei (MN) originating from lagging chromosomes/chromatids at anaphase (mitotic indirect non-disjunction, MIND), and to...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/14.6.557

    authors： Minissi S,Degrassi F,Tanzarella C,Gustavino B

    更新日期：1999-11-01 00:00:00

  • Reversible inhibition of mammalian tubulin assembly in vitro and effects in Saccharomyces cerevisiae D61.M by mitomycin C.

    abstract：:Gaulden reported a novel and unexpected mitomycin C (MMC) effect, namely a pronounced retardation of very late prophase and loss of chromosome orientation in neuroblasts of the grasshopper Chortophaga viridifasciate. Because this effect may be due to interactions of MMC with non-DNA targets, MMC was tested for its int...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/4.1.39

    authors： Albertini S,Friederich U,Würgler FE

    更新日期：1989-01-01 00:00:00

  • The influence of blood storage time and general anaesthesia on chromosomal radiosensitivity assessment.

    abstract：:The micronucleus assay (MN assay) is a well-established assay in genetic toxicology, biomonitoring of mutagen-exposed populations and chromosomal radiosensitivity testing. To evaluate the effect of storage time on the chromosomal radiosensitivity assessment in lymphocytes, micronuclei (MN) yields in blood samples rece...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/gev072

    authors： Baeyens A,Herd O,Francies FZ,Cairns A,Katzman G,Murdoch M,Padiachy D,Morford M,Vral A,Slabbert JP

    更新日期：2016-03-01 00:00:00

  • Sensitivity of Salmonella typhimurium TA97a to the type of agar used for preparation of Vogel-Bonner plates.

    abstract：:Recent problems with the supply of Difco bacto agar have forced some laboratories to evaluate alternative agars for use in the Salmonella/microsome assay. This led to the independent observation in two laboratories (Boots and Glaxo) that Salmonella typhimurium TA97a is sensitive to certain types of agar that may be us...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/7.1.13

    authors： Wilcox P,Wedd DJ,Williams WR,Mee CD,O'Donovan MR

    更新日期：1992-01-01 00:00:00

  • Genotoxicity in mouse lymphoma cells of chemicals capable of Michael addition.

    abstract：:Over the past several years, we have been evaluating the mutagenicity and clastogenicity of compounds capable of Michael-type reactions. These compounds, including acrylamide, several acrylate and methacrylate esters, vinyl sulfones, and phorone, have been evaluated using TK+/- -3.7.2C mouse lymphoma cells. Mutagenic ...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/6.6.519

    authors： Dearfield KL,Harrington-Brock K,Doerr CL,Rabinowitz JR,Moore MM

    更新日期：1991-11-01 00:00:00

  • The DNA repair gene APE1 T1349G polymorphism and cancer risk: a meta-analysis of 27 case-control studies.

    abstract：:Published data regarding the association between the apurinic/apyrimidinic endonuclease 1 (APE1) T1349G (Asp148Glu) polymorphism and cancer risk show inconclusive results. To derive a more precise estimation of the relationship, we performed a meta-analysis of 27 published studies that included 12 432 cancer cases and...

    journal_title：Mutagenesis

    pub_type： 杂志文章,meta分析

    doi：10.1093/mutage/gep036

    authors： Gu D,Wang M,Wang M,Zhang Z,Chen J

    更新日期：2009-11-01 00:00:00

  • Relationships between the DNA adducts and the mutations and sister-chromatid exchanges produced in Chinese hamster ovary cells by N-hydroxy-2-aminofluorene, N-hydroxy-N'-acetylbenzidine and 1-nitrosopyrene.

    abstract：:Chinese hamster ovary cells were exposed to N-hydroxy-2-aminofluorene, N-hydroxy-N'-acetylbenzidine and 1-nitrosopyrene, and the resulting DNA adducts, sister-chromatid exchanges (SCEs) and mutations at the hypoxanthine-guanine phosphoribosyl transferase locus were quantified. Each agent produced a major DNA adduct su...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/1.3.201

    authors： Heflich RH,Morris SM,Beranek DT,McGarrity LJ,Chen JJ,Beland FA

    更新日期：1986-05-01 00:00:00

  • Genome damage in peripheral blood lymphocytes of diabetic and non-diabetic individuals after intervention with vegetables and plant oil.

    abstract：:Recent studies suggest increased cancer risk in patients with type 2 diabetes mellitus (T2DM) compared with healthy individuals. The present study aims to assess whether T2DM is associated with increased genome instability and whether a healthy diet with natural foods can improve genome stability in peripheral blood l...

    journal_title：Mutagenesis

    pub_type： 杂志文章,随机对照试验

    doi：10.1093/mutage/ges073

    authors： Müllner E,Brath H,Toferer D,Adrigan S,Bulla MT,Stieglmayer R,Wallner M,Marek R,Wagner KH

    更新日期：2013-03-01 00:00:00

  • Industrial Genotoxicology Group (IGG): Aneuploidy, White House Hotel, London, UK, 16 May 1997.

    abstract：:Aneuploidy has important consequences for human health. There is a requirement to detect aneugens that may not be fully satisfied by the current test batter. New cytogenetic techniques look promising. ...

    journal_title：Mutagenesis

    pub_type：

    doi：10.1093/mutage/13.4.417

    authors：

    更新日期：1998-07-01 00:00:00

  • Aneuploidies level in sperm nuclei in patients with infertility.

    abstract：:Male infertility is a relevant social and medical problem. Male infertility is mostly caused by genetic disorders. The purpose of the study was to analyze the correlation of chromosome aberrations, as well as DNA fragmentation and various manifestations of spermatogenesis disorder. Sperm samples of 58 males with infer...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/gew020

    authors： Alchinbayev MK,Aralbayeva AN,Tuleyeva LN,Duysenbayeva SM,Makazhanov MA

    更新日期：2016-09-01 00:00:00

  • A preliminary evaluation of the performance of the Muta Mouse (lacZ) and Big Blue (lacI) transgenic mouse mutation assays.

    abstract：:All of the published mutagenicity data generated using the Muta Mouse (lacZ) and the Big Blue (lacI) transgenic mouse mutation assays have been re-presented in a standard format. To date, 26 chemicals have been evaluated in one or other of the assays, and eight tissues have been sampled. The present analysis should ex...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/9.4.367

    authors： Morrison V,Ashby J

    更新日期：1994-07-01 00:00:00

  • Applications of the comet assay in particle toxicology: air pollution and engineered nanomaterials exposure.

    abstract：:Exposure to ambient air particles is associated with elevated levels of DNA strand breaks (SBs) and endonuclease III, formamidopyrimidine DNA glycosylase (FPG) and oxoguanine DNA glycosylase-sensitive sites in cell cultures, animals and humans. In both animals and cell cultures, increases in SB and in oxidatively dama...

    journal_title：Mutagenesis

    pub_type： 杂志文章,评审

    doi：10.1093/mutage/geu035

    authors： Møller P,Hemmingsen JG,Jensen DM,Danielsen PH,Karottki DG,Jantzen K,Roursgaard M,Cao Y,Kermanizadeh A,Klingberg H,Christophersen DV,Hersoug LG,Loft S

    更新日期：2015-01-01 00:00:00

  • Analysis of eight known or suspected aneugens by the in vitro human lymphocyte micronucleus test.

    abstract：:In the light of the CEC aneuploidy programme eight known or suspected aneugens were evaluated with the human lymphocyte 'in vitro' micronucleus test using the cytochalasin-B technique. Only colchicine, chloral hydrate and hydroquinone induced dose-dependent increases in micronucleus frequencies. The other five chemica...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/7.6.447

    authors： Van Hummelen P,Kirsch-Volders M

    更新日期：1992-11-01 00:00:00

  • Disomic and diploid meiotic products induced in Saccharomyces cerevisiae by the salts of 27 elements.

    abstract：:The effects of salts of 27 elements on recombination and on the production of disomic and/or diploid spores during meiosis of Saccharomyces cerevisiae has been investigated. Be(NO3)2, MgSO4, FeSO4, CuSO4, AgNO3, Na2HAsO4 were inactive on the events studied during meiotic cell division. AuCl4, CdCl2, C4H6O4Pb, SnCl2, K...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/1.1.21

    authors： Sora S,Agostoni Carbone ML,Pacciarini M,Magni GE

    更新日期：1986-01-01 00:00:00

  • A new cytogenetic approach for the evaluation of mutagenic potential of chemicals that induce cell cycle arrest in the G2 phase.

    abstract：:The aim of the present study was to develop and standardize a cytogenetic approach for evaluation of the mutagenic potential of chemicals that induce cell cycle arrest in the G2 phase. Even though cytogenetic end-points such as sister chromatid exchange (SCE) have been extensively used to indirectly assess the DNA-dam...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/geg034

    authors： Terzoudi GI,Malik SI,Pantelias GE,Margaritis K,Manola K,Makropoulos W

    更新日期：2003-11-01 00:00:00

  • Role of cerium oxide nanoparticle-induced autophagy as a safeguard to exogenous H2O2-mediated DNA damage in tobacco BY-2 cells.

    abstract：:The effect of cerium oxide nanoparticle (CeNP) in plants has elicited substantial controversy. While some investigators have reported that CeNP possesses antioxidant properties, others observed CeNP to induce reactive oxygen species (ROS). In spite of considerable research carried out on the effects of CeNP in metazoa...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/gey004

    authors： Sadhu A,Ghosh I,Moriyasu Y,Mukherjee A,Bandyopadhyay M

    更新日期：2018-04-13 00:00:00

  • Workers exposed to wood dust have an increased micronucleus frequency in nasal and buccal cells: results from a pilot study.

    abstract：:Wood dust is recognised as a human carcinogen, based on the strong association of wood dust exposure and the elevated risk of malignant tumours of the nasal cavity and paranasal sinuses [sino-nasal cancer (SNC)]. The study aimed to assess genetic damage in workers exposed to wood dust using biomarkers in both buccal a...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/geu003

    authors： Bruschweiler ED,Hopf NB,Wild P,Huynh CK,Fenech M,Thomas P,Hor M,Charriere N,Savova-Bianchi D,Danuser B

    更新日期：2014-05-01 00:00:00

  • The expanding role of mTOR in cancer cell growth and proliferation.

    abstract：:The mechanistic/mammalian target of rapamycin (mTOR) is a conserved protein kinase that controls several anabolic processes required for cell growth and proliferation. As such, mTOR has been implicated in an increasing number of pathological conditions, including cancer, obesity, type 2 diabetes and neurodegeneration....

    journal_title：Mutagenesis

    pub_type： 杂志文章,评审

    doi：10.1093/mutage/geu045

    authors： Cargnello M,Tcherkezian J,Roux PP

    更新日期：2015-03-01 00:00:00

  • Vitamin D3 as adjuvant in the treatment of type 2 diabetes mellitus: modulation of genomic and biochemical instability.

    abstract：:Type 2 diabetes mellitus has undergone a worldwide growth in incidence in the world and has now acquired epidemic status. There is a strong link between type 2 diabetes and vitamin D deficiency. Because vitamin D has beneficial effects on glucose homeostasis, the aim of this study was to evaluate the influence of vita...

    journal_title：Mutagenesis

    pub_type： 杂志文章

    doi：10.1093/mutage/gez001

    authors： Fagundes GE,Macan TP,Rohr P,Damiani AP,Da Rocha FR,Pereira M,Longaretti LM,Vilela TC,Ceretta LB,Mendes C,Silveira PCL,Teixeira JPF,de Andrade VM

    更新日期：2019-05-29 00:00:00