Direct and indirect non-disjunction in the origin of trisomy in cultured human lymphocytes.

abstract：:The micronucleus assay (MN assay) is a well-established assay in genetic toxicology, biomonitoring of mutagen-exposed populations and chromosomal radiosensitivity testing. To evaluate the effect of storage time on the chromosomal radiosensitivity assessment in lymphocytes, micronuclei (MN) yields in blood samples rece...

journal_title：Mutagenesis

authors： Baeyens A,Herd O,Francies FZ,Cairns A,Katzman G,Murdoch M,Padiachy D,Morford M,Vral A,Slabbert JP

更新日期：2016-03-01 00:00:00

abstract：:Alcohol drinking-derived acetaldehyde is believed to cross-link DNA and induce sister chromatid exchanges in peripheral blood lymphocytes. However, little population data are available to illustrate effects of alcohol-derived acetaldehyde on DNA migration as assayed by the comet assay in peripheral lymphocytes. In the...

journal_title：Mutagenesis

authors： Lu Y,Morimoto K

更新日期：2009-07-01 00:00:00

abstract：:The International Agency for Research on Cancer has classified several antineoplastic drugs in Group 1 (human carcinogens), among which chlorambucil, cyclophosphamide (CP) and tamoxifen, Group 2A (probable human carcinogens), among which cisplatin, etoposide, N-ethyl- and N-methyl-N-nitrosourea, and Group 2B (possible...

journal_title：Mutagenesis

authors： Villarini M,Dominici L,Piccinini R,Fatigoni C,Ambrogi M,Curti G,Morucci P,Muzi G,Monarca S,Moretti M

更新日期：2011-05-01 00:00:00

abstract：:The non-syndromic cleft lip and/or palate (NSCL/P) is a common birth defect caused by a combination of genetic and environmental factors. The possible role of genome instability on NSCL/P patient needs more investigation, since DNA metabolism is an essential cellular function to keep cells with normal genotypes and ge...

journal_title：Mutagenesis

authors： Xavier LA,Bezerra JF,de Rezende AA,Oliveira RA,Dalmolin RJ,do Amaral VS

更新日期：2017-03-01 00:00:00

abstract：:Activation of the human bladder carcinogen 4-aminobiphenyl (ABP) and its N-hydroxy derivatives was investigated using lysates and subcellular enzyme preparations from cultured human uroepithelial cells (HUC). Mutagenic activation was determined using Salmonella typhimurium strains TA98; TA98/1,8-DNP6, a derivative def...

journal_title：Mutagenesis

authors： Hatcher JF,Rao KP,Swaminathan S

更新日期：1993-03-01 00:00:00

abstract：:Over the past several years, we have been evaluating the mutagenicity and clastogenicity of compounds capable of Michael-type reactions. These compounds, including acrylamide, several acrylate and methacrylate esters, vinyl sulfones, and phorone, have been evaluated using TK+/- -3.7.2C mouse lymphoma cells. Mutagenic ...

journal_title：Mutagenesis

authors： Dearfield KL,Harrington-Brock K,Doerr CL,Rabinowitz JR,Moore MM

更新日期：1991-11-01 00:00:00

abstract：:Swiss albino mice were exposed to formulated cypermethrin (CMR) and/or or chlorpyrifos (CPF) through oral gavages for 60 days. Test doses of CMR (0.69, 1.38 or 2.76mg/kg/day) or CPF (0.5, 1.0 or 2.0mg/kg/day) or CMR + CPF (0.69 + 0.5, 1.38 + 1.0 or 2.76 + 2.0mg/kg/day) were based on the acute oral median lethal doses ...

journal_title：Mutagenesis

authors： Chauhan LK,Varshney M,Pandey V,Sharma P,Verma VK,Kumar P,Goel SK

更新日期：2016-11-01 00:00:00

abstract：:Deregulation of control of the apoptotic process in Fanconi anaemia (FA) appears to be one of the main features of this disease at the cellular level. We show here that FA cells are resistant to treatments with rhodamine-1,2,3 and doxycycline, which both interfere with mitochondrial functionality by different mechanis...

journal_title：Mutagenesis

authors： Bogliolo M,Borghini S,Abbondandolo A,Degan P

更新日期：2002-01-01 00:00:00

abstract：:An established human hepatoma cell strain (Hep G2) was used in micronuclei (MN) and sister chromatid exchange (SCE) assays to evaluate the clastogenic potential of several indirectly-acting mutagenic carcinogens. Benzo[a]pyrene, cyclophosphamide, dimethyl nitrosamine, hexamethylphosphoramide, pyrene and safrole were s...

journal_title：Mutagenesis

authors： Natarajan AT,Darroudi F

更新日期：1991-09-01 00:00:00

abstract：:Inter-individual differences in susceptibility to breast cancer are partially mediated through the levels of endogenous and exogenous steroid hormones. The CYP17 gene encodes P450c17alpha, an enzyme that is involved in the metabolism of steroid hormones. Increased endogenous steroid hormone levels have been associated...

journal_title：Mutagenesis

authors： Ye Z,Parry JM

更新日期：2002-03-01 00:00:00

abstract：:During the past four years several livestock farms (sheep, cattle and river buffalo) in the provinces of Naples and Caserta (southern Italy) have been unable to sell their milk and other dairy products due to the levels of dioxins (17 different types) present in the milk mass exceeding the value permitted [3 pg/g of f...

journal_title：Mutagenesis

authors： Perucatti A,Di Meo GP,Albarella S,Ciotola F,Incarnato D,Jambrenghi AC,Peretti V,Vonghia G,Iannuzzi L

更新日期：2006-01-01 00:00:00

abstract：:Protection from chronic exposure to cosmic radiation, which is primarily composed of protons, in future manned missions to Mars and beyond is considered to be a key unresolved issue. To model the effects of cosmic radiation on a living cell, we used Saccharomyces cerevisiae cells harboring various deletions of DNA rep...

journal_title：Mutagenesis

authors： Rostek C,Turner EL,Robbins M,Rightnar S,Xiao W,Obenaus A,Harkness TA

更新日期：2008-03-01 00:00:00

abstract：:The principal hydroxy-metabolites of benzene--hydroquinone, catechol and phenol--were assayed in tests for mitotic segregation induction in Aspergillus nidulans diploid strain 19. Hydroquinone was the most effective chemical, increasing the frequency of mitotic segregants up to 10-fold at 1-3 mM. Catechol was similarl...

journal_title：Mutagenesis

authors： Crebelli R,Conti G,Carere A

更新日期：1987-05-01 00:00:00

abstract：:The genotoxic effects of benzene in lung cells of mice exposed to single acute doses by inhalation have been estimated by cytogenetic analysis of micronuclei in primary cultures of lung fibroblasts. Mice were nose-only exposed to 1000 p.p.m. for 30 or 60 min or to 3500 p.p.m. for 30 min and sacrificed 24 h after the e...

journal_title：Mutagenesis

authors： Ranaldi R,Bassani B,Villani P,Lombardi CC,Tanzarella C,Pacchierotti F

更新日期：1998-09-01 00:00:00

abstract：:Aneuploidy has important consequences for human health. There is a requirement to detect aneugens that may not be fully satisfied by the current test batter. New cytogenetic techniques look promising. ...

journal_title：Mutagenesis

authors：

更新日期：1998-07-01 00:00:00

abstract：:We have conducted a randomized trial which investigated the ability of dietary changes (in particular diets rich in cruciferous vegetables and flavonoids), to increase urinary antimutagenicity and inhibit DNA damage in smokers. Ninety heavy smokers were recruited and randomly assigned to three groups, and were given t...

journal_title：Mutagenesis

authors： Talaska G,Al-Zoughool M,Malaveille C,Fiorini L,Schumann B,Vietas J,Peluso M,Munnia A,Bianchini M,Allegro G,Matullo G,Sacerdote C,Vineis P

更新日期：2006-05-01 00:00:00

abstract：:Chromatographic profiles of DNA adducts formed following treatment of Chinese hamster ovary (CHO) cells or C3H10T1/2 mouse embryo fibroblast cells with benzo[a]pyrene (BP), in the presence or absence of rat liver S9 fraction, have been obtained by both direct labelling ([3H]BP) and 32P-post-labelling methods. The prin...

journal_title：Mutagenesis

authors： Watson WP,Smith RJ,Ryan D,Meyer AL

更新日期：1990-07-01 00:00:00

abstract：:The search for micronuclei (MN) in binucleated cells is not always the best choice to recognize microtubule-perturbing agents, as they give rise to (micronucleated) mononucleated cells, mainly via mitotic slippage. We therefore treated peripheral lymphocytes with vincristine (VCR), nocodazole (NOC) and colcemid (COL):...

journal_title：Mutagenesis

authors： Guccini S,Lombardi S,Pisani A,Piaggi S,Scarpato R

更新日期：2012-11-01 00:00:00

abstract：:On April 6, 1993, near the town of Tomsk (Russia) there was an accident at the Siberian Chemical Plant (SCP) which resulted in extensive contamination of an area of 250 km(2) to the north of SCP with long-lived radionuclides such as (239)Pu, (137)Cs and (90)Sr. Cytogenetic methods and electron spin resonance (ESR) spe...

journal_title：Mutagenesis

authors： Ilyinskikh NN,Ilyinskikh IN,Porovskiy VA,Natarajan AT,Suskov II,Smirenniy LN,Ilyinskikh EN

更新日期：1999-09-01 00:00:00

abstract：:The repairability during G(1) of DNA lesions eliciting sister chromatid exchanges (SCE) induced by methylmethanesulfonate (MMS) and ethylmethanesulfonate (EMS) in BrdU-substituted and unsubstituted DNA strands was determined in murine salivary gland cells in vivo. The SCE frequency was determined after exposure to MMS...

journal_title：Mutagenesis

authors： González-Beltrán F,Morales-Ramírez P

更新日期：2003-01-01 00:00:00

abstract：:The clastogenic potential of sodium fluoride was determined both in vitro (using cultured human lymphocytes) and in vivo (using the rat bone-marrow micronucleus test). The incidence of chromosome aberrations in human lymphocyte cultures exposed to 20 or 40 micrograms/ml sodium fluoride (3 and 9% respectively) was sign...

journal_title：Mutagenesis

authors： Albanese R

更新日期：1987-11-01 00:00:00

abstract：:Non-homologous end joining (NHEJ), the major pathway of double-strand break (DSB) repair in mammalian cells, comprises two subpathways: one that requires the three core factors Ku70/80, DNA-PKcs and XRCC4/LigIV (DNA-PK-dependent NHEJ) and the other that is independent of these factors. Using a cell-free NHEJ assay, we...

journal_title：Mutagenesis

authors： Kuhfittig-Kulle S,Feldmann E,Odersky A,Kuliczkowska A,Goedecke W,Eggert A,Pfeiffer P

更新日期：2007-05-01 00:00:00

abstract：:Radiation-induced exchange aberrations are thought to arise as a consequence of misrejoining of free ends of DNA double strand breaks (dsbs). In quiescent mammalian cells this process of misrejoining is prevalently taken up by the non-homologous end joining (NHEJ) process. In order to investigate the role of glutathio...

journal_title：Mutagenesis

authors： Dutta A,Chakraborty A,Saha A,Ray S,Chatterjee A

更新日期：2005-09-01 00:00:00

abstract：:Cell transformation assays (CTAs) have long been proposed as in vitro methods for the identification of potential chemical carcinogens. Despite showing good correlation with rodent bioassay data, concerns over the subjective nature of using morphological criteria for identifying transformed cells and a lack of underst...

journal_title：Mutagenesis

authors： Creton S,Aardema MJ,Carmichael PL,Harvey JS,Martin FL,Newbold RF,O'Donovan MR,Pant K,Poth A,Sakai A,Sasaki K,Scott AD,Schechtman LM,Shen RR,Tanaka N,Yasaei H

更新日期：2012-01-01 00:00:00

abstract：:Niclosamide produces genotoxic effects, such as point mutations in Salmonella sp., sperm-head abnormalities in mice and clastogenic effects in human lymphocytes in vitro and in vivo. As cytochrome P450 could be involved in the bioactivation of niclosamide, we investigated which subfamily was involved. We used liver mi...

journal_title：Mutagenesis

authors： Beristain-Castillo E,Martínez-Vázquez M,Camacho-Carranza R,Espinosa-Aguirre JJ

更新日期：2013-11-01 00:00:00

abstract：:Adducts of 1,2-epoxy-3-butene (EB) with haemoglobin were monitored in 17 workers from the 1,3-butadiene (BD) production unit of a petrochemical plant and in nine referents employed at the same factory but not exposed to BD. The air concentrations of BD were determined using stationary and personal monitoring. The ambi...

journal_title：Mutagenesis

authors： Osterman-Golkar S,Peltonen K,Anttinen-Klemetti T,Landin HH,Zorcec V,Sorsa M

更新日期：1996-03-01 00:00:00

abstract：:I suggest that there are several good reasons for retaining mammalian cell mutation assays in a basic test package for genotoxicity and that the possible existence of a non-clastogenic mammalian mutagen is not one of them. I put forward a number of propositions, not necessarily original, concerning in vitro assays, in...

journal_title：Mutagenesis

authors： Green MH

更新日期：1988-09-01 00:00:00

abstract：:A pronase digestion procedure for the isolation of gastric mucosal cells was evaluated for its usefulness in measuring unscheduled DNA synthesis (UDS). The method has been claimed to be suited for assessing the genotoxicity potential of compounds. Compounds were given orally to rats. After 13 h [3H]thymidine was injec...

journal_title：Mutagenesis

authors： Larsson H,Fryklund J,Helander HF,Wallmark B

更新日期：1991-01-01 00:00:00

abstract：:Sanfetrinem cilexetil, a hexetil ester of a Trinem antibiotic, does not induce micronuclei in rat bone marrow cells or induce DNA repair synthesis in rat hepatocytes following oral dosing. However, in vitro chromosome damage and mutations are induced in mammalian cells lacking carboxylesterase activity (human lymphocy...

journal_title：Mutagenesis

authors： Oliver J,Naidoo A,Vandin L,Pugnaghi F,Gatehouse D,Comelli R

更新日期：2000-01-01 00:00:00

abstract：:We report on a new method for the study of radiation-induced chromatid breaks in stimulated human peripheral blood T lymphocytes, involving a combination of a 1-h colcemid block and a short (15 min) calyculin A treatment. We find that this procedure eliminates the problem of centromere splitting when calyculin A is us...

journal_title：Mutagenesis

authors： Shovman O,Riches AC,Adamson D,Bryant PE

更新日期：2008-07-01 00:00:00