Abstract:
:We report on a new method for the study of radiation-induced chromatid breaks in stimulated human peripheral blood T lymphocytes, involving a combination of a 1-h colcemid block and a short (15 min) calyculin A treatment. We find that this procedure eliminates the problem of centromere splitting when calyculin A is used alone for a longer period and produces metaphase spreads with superior quality. By this procedure, the chromosomes and the chromatid breaks are expanded and thereby make for improved break scoring. In a comparison of the new technique with the conventional colcemid block method, we show a close proportionality between the frequencies of chromatid breaks scored with the two methods. The frequency of chromatid breaks with the new method was found to be significantly higher than that with colcemid alone, adding a higher sensitivity to the assay as an additional advantage.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Shovman O,Riches AC,Adamson D,Bryant PEdoi
10.1093/mutage/gen009subject
Has Abstractpub_date
2008-07-01 00:00:00pages
267-70issue
4eissn
0267-8357issn
1464-3804pii
gen009journal_volume
23pub_type
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