Abstract:
:Coal miners are exposed to coal dust, containing mineral particles, inorganic compounds and polycyclic aromatic hydrocarbons, and to ionizing radiation. These factors can induce oxidative stress and promote inflammation that leads to DNA damage. The aim of this investigation is to analyse the degree of DNA damage in miners working in underground coal mines in Kemerovo Region (Russian Federation) using the cytokinesis-block micronucleus assay (CBMN) in peripheral blood lymphocytes. The exposed group included 143 coal miners (mean age = 50.11±7.36 years; mean length of service in coal mining conditions = 23.26±9.66 years). As a control group, we have used venous blood extracted from 127 healthy non-exposed men. The mean age in this group was 47.67±8.45 years. We have discovered that coal miners are characterized by a significant increase in the frequency of binucleated lymphocytes with micronuclei (MN), nucleoplasmic bridges (NPBs) and protrusions (NBUDs) compared to non-exposed donors. In addition, we report, for the first time, a reduction of cell proliferation in a cohort of coal miners. These data are evidence of the genotoxic and cytostatic effects of occupational harmful factors of the coal mining industry. No correlation between the level of chromosome damage and age, smoking status or length of service in coal mining conditions were discovered. We suggest that the CBMN assay would be useful in biomonitoring studies to monitor hygiene and prevention strategies in occupational settings in coal mining countries.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Sinitsky MY,Minina VI,Gafarov NI,Asanov MA,Larionov AV,Ponasenko AV,Volobaev VP,Druzhinin VGdoi
10.1093/mutage/gew038subject
Has Abstractpub_date
2016-11-01 00:00:00pages
669-675issue
6eissn
0267-8357issn
1464-3804pii
gew038journal_volume
31pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::The aim of the present study was to develop and standardize a cytogenetic approach for evaluation of the mutagenic potential of chemicals that induce cell cycle arrest in the G2 phase. Even though cytogenetic end-points such as sister chromatid exchange (SCE) have been extensively used to indirectly assess the DNA-dam...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geg034
更新日期:2003-11-01 00:00:00
abstract::Male infertility is a relevant social and medical problem. Male infertility is mostly caused by genetic disorders. The purpose of the study was to analyze the correlation of chromosome aberrations, as well as DNA fragmentation and various manifestations of spermatogenesis disorder. Sperm samples of 58 males with infer...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew020
更新日期:2016-09-01 00:00:00
abstract::A perceived disadvantage of transgenic rodent mutation assays is that spontaneous mutant frequencies are high compared to those of endogenous genes and may consequently reduce sensitivity to induced mutation. We have previously argued that unrepaired G:T mismatches from spontaneous deamination of 5-methylcytosine at C...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen026
更新日期:2008-09-01 00:00:00
abstract::3 beta-Hydroxy-13 alpha-amino-13,17-seco-5 alpha-androstan-17-oic-13,17-lactam-p-bis(2-chloroethyl) aminophenoxyacetate (NSC 294859) is a new modified steroidal alkylating agent. This compound was given by i.p. administration to mice bearing different types of tumour. It was found to exhibit good activity in L1210 and...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.5.361
更新日期:1989-09-01 00:00:00
abstract::Alcohol drinking-derived acetaldehyde is believed to cross-link DNA and induce sister chromatid exchanges in peripheral blood lymphocytes. However, little population data are available to illustrate effects of alcohol-derived acetaldehyde on DNA migration as assayed by the comet assay in peripheral lymphocytes. In the...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gep008
更新日期:2009-07-01 00:00:00
abstract::Monitoring oxidative stress in vivo is made easier by the ability to use samples obtained non-invasively, such as urine. The analysis of DNA oxidation, by measurement of oxidized 2'-deoxyribonucleosides in urine, particularly 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), has been reported extensively in the literatur...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq031
更新日期:2010-09-01 00:00:00
abstract::The restriction site mutation method described by Parry et al. (1990) for isolating mutant sequences directly from DNA is discussed and an additional enrichment step proposed using magnetic beads. ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/5.6.523
更新日期:1990-11-01 00:00:00
abstract::We employed the three-way differential staining technique (TWD), which allows SCEs to be distinguished on a per generation basis by scoring third metaphases (M3), in order to study the spontaneous levels of SCEs in normal and high frequency cells (HFCs) that occurred in the first (S1), second (S2) and third (S3) S pha...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/12.5.329
更新日期:1997-09-01 00:00:00
abstract::Exposure to ambient air particles is associated with elevated levels of DNA strand breaks (SBs) and endonuclease III, formamidopyrimidine DNA glycosylase (FPG) and oxoguanine DNA glycosylase-sensitive sites in cell cultures, animals and humans. In both animals and cell cultures, increases in SB and in oxidatively dama...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/geu035
更新日期:2015-01-01 00:00:00
abstract::A transgenic mouse strain with a high copy number of rescuable lacZ sequences was evaluated for its effectiveness in detecting lacZ- mutations in selected tissues. Procarbazine, cyclophosphamide, ethylnitrosourea, 7,12-dimethylbenz[a]anthracene (DMBA), acrylamide and chlorambucil were tested following either single or...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.1.7
更新日期:1993-01-01 00:00:00
abstract::The micronucleus test (MNT) is a well-established assay in genotoxicity testing and human biomonitoring. The cytokinesis-block micronucleus test (CBMNT) is the preferred method for measuring MN in cultured human lymphocytes from human subjects exposed to genotoxins. However, it is unclear to what extent mutagen exposu...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/geq061
更新日期:2011-01-01 00:00:00
abstract::Nitropyrenes are a class of polycyclic hydrocarbons that have been shown to be potent point mutagens in the Salmonella/mammalian microsome assay. However, relatively few data have been published on their ability to induce chromosome aberrations. In this paper we report the cytogenetic activity of 1-nitropyrene, 1-nitr...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.1.23
更新日期:1987-01-01 00:00:00
abstract::Three short-term assays (the SOS chromotest, the Ames-fluctuation test and the newt micronucleus test) were carried out to evaluate the genotoxicity of four trihalomethanes (chloroform, bromodichloromethane, chlorodibromomethane and bromoform). With the SOS chromotest, all the chemicals studied except chloroform were ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/10.4.333
更新日期:1995-07-01 00:00:00
abstract::Oligodontia, which is the congenital absence of six or more permanent teeth, excluding the third molars, may contribute to masticatory dysfunction, speech alteration, aesthetic problems and malocclusion. Msh homeobox 1 (MSX1) was the first gene identified as causing non-syndromic oligodontia. In this study, we identif...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geu019
更新日期:2014-09-01 00:00:00
abstract::In response to DNA damage, cell cycle arrest, apoptosis, and DNA repair are mediated by a TP53 pathway that induces p21(WAF1/Cip1). The chemotherapeutic drug cis-diamminedichloroplatinum-II (cisplatin) damages cellular DNA by forming cis-diammineplatinum-N(7)-d[GpG] and cis-diammine-platinum-N(7)-d[ApG] adducts. To in...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gel050
更新日期:2007-01-01 00:00:00
abstract::Despite their prime candidate status, polymorphisms near genes involved in DNA repair or in other functions related to genome stability have been conspicuously under-represented in the significant associations reported from genome-wide association studies (GWAS) of cancer susceptibility. In this study, we assessed a s...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ger070
更新日期:2012-03-01 00:00:00
abstract::Activation of the human bladder carcinogen 4-aminobiphenyl (ABP) and its N-hydroxy derivatives was investigated using lysates and subcellular enzyme preparations from cultured human uroepithelial cells (HUC). Mutagenic activation was determined using Salmonella typhimurium strains TA98; TA98/1,8-DNP6, a derivative def...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.2.113
更新日期:1993-03-01 00:00:00
abstract::Due to the very limited transduction capacity of hitherto available vectors, the success of gene therapy of tumours by means of suicide genes has so far essentially depended on the transfer of toxic drug metabolites from transduced (metabolizing) cells to adjacent non-transduced cells via gap junctions (bystander effe...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geh002
更新日期:2004-01-01 00:00:00
abstract::As part of the hazard and risk assessment of chemicals in man, it is important to assess the ability of a chemical to induce mutations in vivo. Because of the commonalities in the molecular initiating event, mutagenicity in vitro can correlate well to the in vivo endpoint for certain compound classes; however, the dif...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gey030
更新日期:2019-03-06 00:00:00
abstract::The old herbal drug aristolochic acid (AA), derived from Aristolochia spp., has been associated with the development of a novel nephropathy, designated aristolochic acid nephropathy (AAN), and urothelial cancer in AAN patients. There is clear evidence that the major components of the plant extract AA, aristolochic aci...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/17.4.265
更新日期:2002-07-01 00:00:00
abstract::Blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) is an autosomal dominant disorder characterized by blepharophimosis, ptosis and epicanthus inversus. Based on the presence and absence of premature ovarian failure, two clinical types have been distinguished. Both types of BPES have been mapped to chromosome ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei067
更新日期:2006-01-01 00:00:00
abstract::Tobacco farming is an important economic income in Brazil, although it has been challenged as regard the occupational exposure to both pesticides and nicotine endured by farmers. Chronic occupational exposure to complex mixtures can lead to health hazardous. We examined genomic instability and epigenetic changes in to...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gex045
更新日期:2018-04-13 00:00:00
abstract::The micronucleus assay (MN assay) is a well-established assay in genetic toxicology, biomonitoring of mutagen-exposed populations and chromosomal radiosensitivity testing. To evaluate the effect of storage time on the chromosomal radiosensitivity assessment in lymphocytes, micronuclei (MN) yields in blood samples rece...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev072
更新日期:2016-03-01 00:00:00
abstract::The cytokinesis-block micronucleus cytome (CBMNCyt) assay is a widely used technique for measuring DNA damage in human populations. The formation of micronuclei (MN) in dividing cells can result from chromosome breakage due to unrepaired or mis-repaired DNA lesions or chromosome malsegregation due to mitotic malfuncti...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/geq076
更新日期:2011-01-01 00:00:00
abstract::The tetrahydrofuran-type abasic site analog (THF) induces large deletion mutations in human cells. To compare the large deletions induced by THF on leading and lagging strand templates, plasmid DNAs bearing the analog at a specific position outside the supF gene were introduced into human U2OS cells. The replicated DN...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gez034
更新日期:2019-12-19 00:00:00
abstract::Hepatic microsomes from rats pretreated with PCB were found to metabolize the food mutagen 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) to two major metabolites, one of which was identified as the N-hydroxy derivative, 2-hydroxy-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (N-OH-PhIP). This identification w...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/6.1.93
更新日期:1991-01-01 00:00:00
abstract::Five selectively methylated analogs of the flame retardant tris(2,3-dibromopropyl)phosphate (Tris-BP) and of the nematocide 1,2-dibromo-3-chloropropane (DBCP) were synthesized and their relative mutagenicities determined in Salmonella typhimurium TA100 in the presence of rat liver microsomes. In all cases, methylation...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.4.287
更新日期:1987-07-01 00:00:00
abstract::Detailed dose-response data for two reference aneugens, vincristine and nocodazole, have been derived for the mouse bone marrow micronucleus assay. Positive data have also been described for six other micronucleus-inducing agents: cyclophosphamide; aniline; urethane; 1,2-dimethylhydrazine; 7,12-dimethylbenzanthracene ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/6.3.193
更新日期:1991-05-01 00:00:00
abstract::A substantial database on hprt mutant frequency in circulating T-cells derived from 153 normal humans was analysed to determine if any effect of alcohol consumption could be detected. Alcohol intake (as reported in a questionnaire completed at the time of the blood sample) ranged from 0 to 35+ units per week. Donors w...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/10.5.449
更新日期:1995-09-01 00:00:00
abstract::The effect of cerium oxide nanoparticle (CeNP) in plants has elicited substantial controversy. While some investigators have reported that CeNP possesses antioxidant properties, others observed CeNP to induce reactive oxygen species (ROS). In spite of considerable research carried out on the effects of CeNP in metazoa...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gey004
更新日期:2018-04-13 00:00:00