Abstract:
:Hepatic microsomes from rats pretreated with PCB were found to metabolize the food mutagen 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (PhIP) to two major metabolites, one of which was identified as the N-hydroxy derivative, 2-hydroxy-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine (N-OH-PhIP). This identification was based on mass spectral (MS), UV and HPLC data by comparison with N-OH-PhIP prepared by chemical synthesis, as well as the specific activity of the compound in the Ames Salmonella test. Synthetic N-OH-PhIP was prepared by catalytic reduction of the nitro derivative of PhIP, which was synthesized from PhIP by diazotization and reaction with sodium nitrite. N-OH-PhIP was mutagenic to Salmonella typhimurium TA98 without metabolic activation and had a specific mutagenic activity of 2700 revertants/nmol. N-OH-PhIP thus seems to be a proximate mutagenic metabolite of PhIP. Other direct acting mutagens were not detected in the microsomal incubation mixture after HPLC separation. N-OH-PhIP also induced sister chromatid exchange (SCE) in Chinese hamster ovary cells (CHO cells) without metabolic activation. The specific activity of N-OH-PhIP in this assay was approximately 3 times higher than the activity of PhIP with microsomal activation.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Frandsen H,Rasmussen ES,Nielsen PA,Farmer P,Dragsted L,Larsen JCdoi
10.1093/mutage/6.1.93subject
Has Abstractpub_date
1991-01-01 00:00:00pages
93-8issue
1eissn
0267-8357issn
1464-3804journal_volume
6pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::The effects of deficiency in the DNA mismatch repair (MMR) protein Pms2 were investigated using the endogenous mouse Hprt and Tk genes as reporters of intragenic mutation and loss of heterozygosity (LOH). Pms2(-/-)Tk(+/-), Pms2(+/+)Tk(+/-), Pms2(+/-)Tk(+/-) and Pms2(-/-)Tk(-/-) mice were bred from Pms2(+/-)Tk(+/-) mic...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geg007
更新日期:2003-07-01 00:00:00
abstract::This is a review of the work on molecular dosimetry carried out by several laboratories within the third Environmental Programme of the Commission of the European Communities. The molecular dosimetry project is a coordinated research project in which several laboratories cooperated on various aspects of quantitative c...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/3.3.179
更新日期:1988-05-01 00:00:00
abstract::The non-syndromic cleft lip and/or palate (NSCL/P) is a common birth defect caused by a combination of genetic and environmental factors. The possible role of genome instability on NSCL/P patient needs more investigation, since DNA metabolism is an essential cellular function to keep cells with normal genotypes and ge...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew068
更新日期:2017-03-01 00:00:00
abstract::AHH-1 TK+/- is a human B cell-derived lymphoblastoid cell line that constitutively expresses a high level of the cytochrome CYP1A1. The MCL-5 cell line was developed by transfection of AHH-1 with cDNAs encoding the human cytochrome P450s, CYP1A2, CYP2A6, CYP2E1, CYP3A4 and microsomal epoxide hydrolase carried in plasm...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.4.417
更新日期:1999-07-01 00:00:00
abstract::Colcemid (COM) and econazole (EZ) were tested for induction of mitotic arrest and C-mitotic effects in mouse bone marrow cells and for meiotic delay and induction of hyperploidy in mouse spermatocytes after single injection. Doses of 1 and 3 mg/kg COM were used and bone marrow and spermatocytes were sampled at 2, 6, 1...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.4.409
更新日期:1998-07-01 00:00:00
abstract::The objective of the present study was to develop a cellular phenotype assay for nucleotide excision repair (NER), using benzo[a]pyrene diol epoxide (BPDE) as model mutagen. Since in vitro exposure to BPDE may lead to DNA strand breaks resulting from both direct interaction with DNA and incisions introduced by the rep...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gep039
更新日期:2010-01-01 00:00:00
abstract::The International Agency for Research on Cancer has classified several antineoplastic drugs in Group 1 (human carcinogens), among which chlorambucil, cyclophosphamide (CP) and tamoxifen, Group 2A (probable human carcinogens), among which cisplatin, etoposide, N-ethyl- and N-methyl-N-nitrosourea, and Group 2B (possible...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq102
更新日期:2011-05-01 00:00:00
abstract::Isothiocyanates are plant-derived compounds that may be beneficial in the prevention of certain chronic diseases. Yet, by stimulating the production of reactive oxygen species (ROS), isothiocyanates can be genotoxic. Whether antioxidants influence isothiocyanate-induced genotoxicity is unclear, but this situation was ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev003
更新日期:2015-05-01 00:00:00
abstract::Zeocin is a member of the bleomycin/phleomycin family of antibiotics, known to bind and cleave DNA. We established human SK-OV-3 cells that stably express the Zeocin resistance gene (Sh ble) using an ecdysone-inducible mammalian expression system. Surprisingly, our results demonstrated that Zeocin, added in the cultur...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei016
更新日期:2005-03-01 00:00:00
abstract::The repairability during G(1) of DNA lesions eliciting sister chromatid exchanges (SCE) induced by methylmethanesulfonate (MMS) and ethylmethanesulfonate (EMS) in BrdU-substituted and unsubstituted DNA strands was determined in murine salivary gland cells in vivo. The SCE frequency was determined after exposure to MMS...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/18.1.13
更新日期:2003-01-01 00:00:00
abstract::Genotoxic stress causes a reduced stability of the plant genome and has a detrimental effect on plant growth and productivity. Double-strand breaks (DSBs) are the most harmful of all DNA lesions because they cause the loss of genetic information on both strands of the DNA helix. In the presented study the coding and g...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev033
更新日期:2015-11-01 00:00:00
abstract::The principal hydroxy-metabolites of benzene--hydroquinone, catechol and phenol--were assayed in tests for mitotic segregation induction in Aspergillus nidulans diploid strain 19. Hydroquinone was the most effective chemical, increasing the frequency of mitotic segregants up to 10-fold at 1-3 mM. Catechol was similarl...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.3.235
更新日期:1987-05-01 00:00:00
abstract::Primary liver fibroblasts were applied in a cytokinesis-block micronucleus assay in combination with fluorescence in situ hybridization (FISH) using two protocols. In protocol A (Prot. A), cytochalasin B (Cyt B) was added at the end of the treatment time directly to the medium containing the standard compounds, wherea...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/15.2.143
更新日期:2000-03-01 00:00:00
abstract::Famotidine, a histamine H2-receptor antagonist, was devoid of mutagenic activity in seven his- Salmonella typhimurium strains (TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102) and was equitoxic in repair-proficient (WP2) and repair-deficient (WP2uvrA, WP67, CM561, CM571, WP100 and CM871) Escherichia coli strains, ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/1.2.125
更新日期:1986-03-01 00:00:00
abstract::Fanconi anaemia (FA) patients show cellular sensitivity to a variety of clastogens and prominently to cross-linking agents. Although there is a long-standing clinical impression of radiosensitivity, in vitro studies have yielded conflicting results. In this study, initial radiation-induced DNA damage and kinetics of D...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen052
更新日期:2009-01-01 00:00:00
abstract::The mechanism responsible for the modification of mutagenicity by chlorophyllin has been investigated using mutagenic compounds with different mechanisms of action, including the monofunctional alkylating agents, N-methyl-N'-nitrosourea (MNU) and ethylmethanesulphonate (EMS); nitrosamines related to tobacco products, ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/7.5.349
更新日期:1992-09-01 00:00:00
abstract::The dose effect between nucleoplasmic bridges (NPB) and relatively low doses of ionising radiation remains unknown. Accordingly, this study investigated the NPB frequencies in human peripheral blood lymphocytes exposed to low-dose (60)Co γ-rays. Complex anomalies, including fused nuclei (FUS), horse-shoe nuclei (HS) a...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew001
更新日期:2016-07-01 00:00:00
abstract::Detailed dose-response data for two reference aneugens, vincristine and nocodazole, have been derived for the mouse bone marrow micronucleus assay. Positive data have also been described for six other micronucleus-inducing agents: cyclophosphamide; aniline; urethane; 1,2-dimethylhydrazine; 7,12-dimethylbenzanthracene ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/6.3.193
更新日期:1991-05-01 00:00:00
abstract::Potential toxicological interactions of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and/or dibutyl phthalate (DBP) with ozone were investigated. Male and female B6C3F1 mice were exposed to ozone (0.5 p.p.m.), NNK (1.0 mg/kg), DBP (5000 p.p.m.) and different combinations of these toxicants 6 h/day for 16,...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/17.4.331
更新日期:2002-07-01 00:00:00
abstract::The partial hepatectomy method, co-treatment method with mitogens and an in vivo/in vitro assay method have been reported as in vivo liver micronucleus (MN) assays. These methods have disadvantages with respect to widespread use as an in vivo assay, i.e. they are time consuming, labour intensive and there is the possi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen047
更新日期:2009-01-01 00:00:00
abstract::The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent n...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.2.249
更新日期:1999-03-01 00:00:00
abstract::Barrett's oesophagus (BO) carries an increased risk of progression to oesophageal adenocarcinoma. Chromoendoscopy with methylene blue (MB) can be used to facilitate identification of BO and target areas for biopsy. If photoexcited, MB can generate reactive oxygen species and genotoxic photodegradation products leading...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gep004
更新日期:2009-05-01 00:00:00
abstract:UNLABELLED:Gastric cancer is the second leading cause of cancer-related death worldwide with a low 5-year survival (S5y) after initial diagnosis. Although aberrant Wnt/β-catenin (CTNNB1) signaling has been observed in multiple human cancers, there is no information on the role of CTNNB1 polymorphisms in gastric cancer ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ges027
更新日期:2012-11-01 00:00:00
abstract::The present study was undertaken to compare the antimutagenic activity of aqueous extracts, at the concentrations used for human consumption, from green, black and decaffeinated black tea. Antimutagenic potential was evaluated against three indirect-acting dietary carcinogens, Glu-P-1, benzo(a)pyrene and nitrosopyrrol...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.6.597
更新日期:1996-11-01 00:00:00
abstract::In order to discriminate between different mechanisms put forward to explain the low observed yields of reciprocal translocation configurations at diakinesis-metaphase I spermatocytes following treatment of mouse stem cell spermatogonia with chemical clastogens, the physiological states of stem cells at the moment of ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/7.4.271
更新日期:1992-07-01 00:00:00
abstract::Non-cytotoxic concentrations (1.5, 3 and 6 microM) of sodium arsenite (SA) were used to study its cytogenetic effects with special reference to the frequency and nature of chromosomal aberrations on the X chromosome at Xq21 and at centromeric regions in CHO9, EM-C11, V79, V-H4 and CHE cell lines using Giemsa and FISH ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.3.229
更新日期:1998-05-01 00:00:00
abstract::Recent problems with the supply of Difco bacto agar have forced some laboratories to evaluate alternative agars for use in the Salmonella/microsome assay. This led to the independent observation in two laboratories (Boots and Glaxo) that Salmonella typhimurium TA97a is sensitive to certain types of agar that may be us...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/7.1.13
更新日期:1992-01-01 00:00:00
abstract::The major risk factor for skin cancer is exposure to UV radiation from sunlight, but other environmental exposures may also play a role in combination with UV. We have studied the effects of combined exposure of primary human skin cells in vitro to UVA, UVB or UVC with benzo[a] pyrene (BaP), an environmental carcinoge...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei042
更新日期:2005-07-01 00:00:00
abstract::The nucleoside analog zidovudine (3'-azido-3'-deoxythymidine, AZT), by itself or in combination with other anti- retroviral drugs, is used perinatally to prevent mother to child transmission of human immunodeficiency virus type 1. AZT is mutagenic in vitro and mutagenic and carcinogenic when administered to neonatal m...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geh033
更新日期:2004-07-01 00:00:00
abstract::A relatively large, reproducible inter-individual variation was found in the ability of 17 human liver S9 samples to mediate the mutagenicity of 2-acetylaminofluorene (2AAF) in Salmonella typhimurium strain TA98. In an animal model, variation in metabolic activation of 2AAF did not appear to relate to the phenotype of...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/3.4.323
更新日期:1988-07-01 00:00:00