Abstract:
:Zeocin is a member of the bleomycin/phleomycin family of antibiotics, known to bind and cleave DNA. We established human SK-OV-3 cells that stably express the Zeocin resistance gene (Sh ble) using an ecdysone-inducible mammalian expression system. Surprisingly, our results demonstrated that Zeocin, added in the culture medium to maintain the expression of the ecdysone receptor, was responsible for the formation of DNA strand breaks in the recombinant cells. This suggests that the Zeocin is not completely detoxified and is still able to cleave DNA, despite the stable expression of the Sh ble gene in the recombinant clones. Our study indicates that one needs to be very cautious in the interpretation of data involving stable cell lines selected with Zeocin.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Oliva-Trastoy M,Defais M,Larminat Fdoi
10.1093/mutage/gei016subject
Has Abstractpub_date
2005-03-01 00:00:00pages
111-4issue
2eissn
0267-8357issn
1464-3804pii
gei016journal_volume
20pub_type
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