Abstract:
:Extracts from lean pork heated at 200 degrees C have a strong mutagenic activity in the Ames Salmonella assay (strain TA98 +S9). The formation of mutagenicity is highly temperature dependent, thus an extract heated at 100 degrees C is not mutagenic in this system. This paper shows that the 200 degrees C extract also causes mutations at the hprt locus in normal human fibroblasts, as demonstrated by a dose-dependent increase of 6-thioguanine resistant mutants. The mutation frequencies were increased 6 and 13 times, respectively, for extract concentrations corresponding to 100 and 200 mg meat/ml medium. Heterocyclic amines, previously shown to be present in the 200 degrees C extract are conceivably responsible for at least part of the observed mutagenicity. The extracts prepared at 100 degrees C had no significant effect on the mutant frequency in human fibroblasts. A pronounced, dose-dependent, decrease in cell survival was observed with both the 100 and the 200 degrees C extracts. The nature of the cytotoxic components is not clear and might be different in the two extracts.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Kolman A,Kleman M,Bohusová T,Gustafsson JA,Overvik Edoi
10.1093/mutage/7.2.141subject
Has Abstractpub_date
1992-03-01 00:00:00pages
141-4issue
2eissn
0267-8357issn
1464-3804journal_volume
7pub_type
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