Abstract:
:Studies in mono-culture of cells have shown that diesel exhaust particles (DEPs) increase the production of reactive oxygen species (ROS) and oxidative stress-related damage to DNA. However, the level of particle-generated genotoxicity may depend on interplay between different cell types, e.g. lung epithelium and immune cells. Macrophages have important immune defence functions by engulfing insoluble foreign materials, including particles, although they might also promote or enhance inflammation. We investigated the effect of co-culturing type II lung epithelial A549 cells with macrophages upon treatment with standard reference DEPs, SRM2975 and SRM1650b. The exposure to DEPs did not affect the colony-forming ability of A549 cells in co-culture with THP-1a cells. The DEPs generated DNA strand breaks and oxidatively damaged DNA, measured using the alkaline comet assay as formamidopyrimidine-DNA glycosylase or oxoguanine DNA glycosylase (hOGG1) sensitive sites, in mono-cultures of A549 or THP-1a and co-cultures of A549 and THP-1a cells. The strongest genotoxic effects were observed in A549 mono-cultures and SRM2975 was more potent than SRM1650b. The ROS production only increased in cells exposed to SRM2975, with strongest concentration-dependent effect in the THP-1a mono-cultures. The basal respiration level in THP-1a cells increased on exposure to SRM1650b and SRM2975 without indication of mitochondrial dysfunction. This is consistent with activation of the cells and there was no direct relationship between levels of respiration and ROS production. In conclusion, exposure of mono-cultured cells to DEPs generated oxidative stress to DNA, whereas co-cultures with macrophages had lower levels of oxidatively damaged DNA than A549 epithelial cells.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Jantzen K,Roursgaard M,Desler C,Loft S,Rasmussen LJ,Møller Pdoi
10.1093/mutage/ges035subject
Has Abstractpub_date
2012-11-01 00:00:00pages
693-701issue
6eissn
0267-8357issn
1464-3804pii
ges035journal_volume
27pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::Hyperbaric oxygen (HBO) treatment as used therapeutically has been shown to induce DNA damage in the alkaline comet assay with leukocytes from test subjects. Using formamidopyrimidine-DNA glycosylase, a DNA repair enzyme which specifically nicks DNA at sites of 8-oxoguanines and formamidopyrimidines, we have detected ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.1.85
更新日期:1998-01-01 00:00:00
abstract::The micronucleus assay (MN assay) is a well-established assay in genetic toxicology, biomonitoring of mutagen-exposed populations and chromosomal radiosensitivity testing. To evaluate the effect of storage time on the chromosomal radiosensitivity assessment in lymphocytes, micronuclei (MN) yields in blood samples rece...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev072
更新日期:2016-03-01 00:00:00
abstract::During the past four years several livestock farms (sheep, cattle and river buffalo) in the provinces of Naples and Caserta (southern Italy) have been unable to sell their milk and other dairy products due to the levels of dioxins (17 different types) present in the milk mass exceeding the value permitted [3 pg/g of f...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei076
更新日期:2006-01-01 00:00:00
abstract::AHH-1 TK+/- is a human B cell-derived lymphoblastoid cell line that constitutively expresses a high level of the cytochrome CYP1A1. The MCL-5 cell line was developed by transfection of AHH-1 with cDNAs encoding the human cytochrome P450s, CYP1A2, CYP2A6, CYP2E1, CYP3A4 and microsomal epoxide hydrolase carried in plasm...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.4.417
更新日期:1999-07-01 00:00:00
abstract::The Mre11 complex (Mre11-Rad50-Nbs1) is involved in a diverse array of DNA metabolic processes including the response to DNA double-strand breaks (DSBs). The structure of Rad50 plays a key role in the DNA-binding and end-bridging activity of the complex. An interesting feature within the central portion of the Rad50 p...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gem011
更新日期:2007-07-01 00:00:00
abstract::We have investigated the genotoxicity of lambda-cyhalothrin (LCT) (CAS registry No. 91465-08-06), a pyrethroid insecticide, in bone marrow cells and in colonic crypt epithelial cells of groups of four rats per dose treated in vivo by gavage at doses of 0.8, 3.06 and 6.12 mg/kg body weight (body wt). We measured genoto...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei020
更新日期:2005-03-01 00:00:00
abstract::Photomutagenicity assays are required for regulatory submissions of some chemicals. As yet there are no well-validated protocols available for these assays. Critical factors which may contribute to the ability of a bacterial assay to detect photomutagens (e.g. dose of UV and test chemical, exposure conditions, light s...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/9.5.459
更新日期:1994-09-01 00:00:00
abstract::Macrocyclic polyethers (crown ethers) are a family of compounds which possess the ability to transport ionic species across natural and artificial membranes. Because of this characteristic, they have wide applications in industry and are being investigated for their potential as pharmacologic agents. However, these co...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.6.437
更新日期:1989-11-01 00:00:00
abstract::The induction of localized DNA damage within a discrete nuclear volume is an important tool in DNA repair studies. Both charged particle irradiation and laser microirradiation (LMI) systems allow for such a localized damage induction, but the results obtained are difficult to compare, as the delivered laser dose canno...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq005
更新日期:2010-05-01 00:00:00
abstract::In Saccharomyces cerevisiae, disruption of genes by deletion allowed elucidation of the molecular mechanisms of a series of human diseases, such as in Wilson disease (WD). WD is a disorder of copper metabolism, due to inherited mutations in human copper-transporting ATPase (ATP7B). An orthologous gene is present in S....
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev062
更新日期:2016-01-01 00:00:00
abstract::A modified protocol is described for the in vitro analysis of micronuclei in whole blood or separated lymphocyte cultures. The induction of binucleate cells by various concentrations of cytochalasin B (3, 4.5 or 6 micrograms/ml) was examined at two harvest times (68 or 72 h). An optimal yield was obtained by adding cy...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.4.317
更新日期:1993-07-01 00:00:00
abstract::The thymidine kinase-deficient subclone, 707BUF, of the Friend murine leukaemia cell line exhibits increased sensitivity to the induction of cytogenetic aberrations by mitomycin C (MMC) relative to wild-type clone 707. It has been suggested that thymidine kinase-deficient cells may be highly mutagen-sensitive through ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/1.3.173
更新日期:1986-05-01 00:00:00
abstract::The non-syndromic cleft lip and/or palate (NSCL/P) is a common birth defect caused by a combination of genetic and environmental factors. The possible role of genome instability on NSCL/P patient needs more investigation, since DNA metabolism is an essential cellular function to keep cells with normal genotypes and ge...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew068
更新日期:2017-03-01 00:00:00
abstract::Potential toxicological interactions of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and/or dibutyl phthalate (DBP) with ozone were investigated. Male and female B6C3F1 mice were exposed to ozone (0.5 p.p.m.), NNK (1.0 mg/kg), DBP (5000 p.p.m.) and different combinations of these toxicants 6 h/day for 16,...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/17.4.331
更新日期:2002-07-01 00:00:00
abstract::We have conducted a randomized trial which investigated the ability of dietary changes (in particular diets rich in cruciferous vegetables and flavonoids), to increase urinary antimutagenicity and inhibit DNA damage in smokers. Ninety heavy smokers were recruited and randomly assigned to three groups, and were given t...
journal_title:Mutagenesis
pub_type: 杂志文章,随机对照试验
doi:10.1093/mutage/gel021
更新日期:2006-05-01 00:00:00
abstract::The toxicological relevance of the micronucleus (MN) test is well defined: it is a multi-target genotoxic endpoint, assessing not only clastogenic and aneugenic events but also some epigenetic effects, which is simple to score, accurate, applicable in different cell types. In addition, it is predictive for cancer, ame...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/geq068
更新日期:2011-01-01 00:00:00
abstract::The clastogenic potential of sodium fluoride was determined both in vitro (using cultured human lymphocytes) and in vivo (using the rat bone-marrow micronucleus test). The incidence of chromosome aberrations in human lymphocyte cultures exposed to 20 or 40 micrograms/ml sodium fluoride (3 and 9% respectively) was sign...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.6.497
更新日期:1987-11-01 00:00:00
abstract::In the light of the CEC aneuploidy programme eight known or suspected aneugens were evaluated with the human lymphocyte 'in vitro' micronucleus test using the cytochalasin-B technique. Only colchicine, chloral hydrate and hydroquinone induced dose-dependent increases in micronucleus frequencies. The other five chemica...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/7.6.447
更新日期:1992-11-01 00:00:00
abstract::There are several reports demonstrating that aneugens may preferentially affect segregation of particular chromosomes in somatic cells. Much less is known on specific susceptibility of individual chromosomes to non-disjunction in mammalian meiosis in response to chemical exposures. To explore possible chromosome-speci...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/16.5.407
更新日期:2001-09-01 00:00:00
abstract::The human lymphocyte micronucleus (MN) test combined with fluorescence in situ hybridization (FISH) of a centromeric probe is considered a useful screening assay to distinguish between clastogenic and aneugenic agents. Four suspected aneuploidy-inducing chemicals, acetaldehyde (AA), diethylstilbestrol (DES), diethylst...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.3.285
更新日期:1996-05-01 00:00:00
abstract::The partial hepatectomy method, co-treatment method with mitogens and an in vivo/in vitro assay method have been reported as in vivo liver micronucleus (MN) assays. These methods have disadvantages with respect to widespread use as an in vivo assay, i.e. they are time consuming, labour intensive and there is the possi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen047
更新日期:2009-01-01 00:00:00
abstract::Protection from chronic exposure to cosmic radiation, which is primarily composed of protons, in future manned missions to Mars and beyond is considered to be a key unresolved issue. To model the effects of cosmic radiation on a living cell, we used Saccharomyces cerevisiae cells harboring various deletions of DNA rep...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gem055
更新日期:2008-03-01 00:00:00
abstract::Genotoxic stress causes a reduced stability of the plant genome and has a detrimental effect on plant growth and productivity. Double-strand breaks (DSBs) are the most harmful of all DNA lesions because they cause the loss of genetic information on both strands of the DNA helix. In the presented study the coding and g...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev033
更新日期:2015-11-01 00:00:00
abstract::A perceived disadvantage of transgenic rodent mutation assays is that spontaneous mutant frequencies are high compared to those of endogenous genes and may consequently reduce sensitivity to induced mutation. We have previously argued that unrepaired G:T mismatches from spontaneous deamination of 5-methylcytosine at C...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen026
更新日期:2008-09-01 00:00:00
abstract::The human JC virus (JCV) is potentially carcinogenic to humans as a Group 2B carcinogen, and it is ubiquitous in human populations. To investigate whether the small tumour (ST) antigen of the JCV contributes to genomic instability, we established cell lines stably expressing the JCV ST and examined its role in DNA rep...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev004
更新日期:2015-07-01 00:00:00
abstract::The adaptive response is a phenomenon by which cells exposed to low, non-cytotoxic doses of a genotoxicant become significantly resistant to a subsequent higher dose of the same or another genotoxic agent. Induction of the adaptive response has been mainly studied using ionizing radiation and alkylating agents as geno...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/12.6.457
更新日期:1997-11-01 00:00:00
abstract::The importance of diet in DNA damage prevention is well established; however, the comparison of weight loss diets with different micronutrient and macronutrient profiles on genome stability in peripheral blood lymphocytes (PBLs) has not been studied. This study tested the hypothesis that genome stability in PBLs of ov...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gep006
更新日期:2009-05-01 00:00:00
abstract::FD&C Red No.3 (erythrosine) has been used as a dye in foods, drugs and cosmetics since its approval by the US Department of Agriculture in 1907. In 1977 the Certified Color Manufacturers' Association (CCMA) initiated studies on FD&C Red No.3 including chronic toxicity and carcinogenicity studies in rats and mice. Data...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/1.4.253
更新日期:1986-07-01 00:00:00
abstract::The genotoxicity of alpha-hexachlorocyclohexane (alpha-HCH) was evaluated in primary cultures of mouse, rat and human hepatocytes. DNA fragmentation was measured by the alkaline elution technique and DNA repair synthesis by quantitative autoradiography. A 20 h exposure to subtoxic concentrations ranging from 0.056 to ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.1.79
更新日期:1996-01-01 00:00:00
abstract::Five independently isolated glutathione-deficient (gsh-) mutants of Saccharomyces cerevisae with maximally 6% residual glutathione content have been analysed genetically. Complementation as well as tetrad analysis of the homo- and heterozygous diploids constructed by suitable crosses of the five mutants indicated that...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/5.1.39
更新日期:1990-01-01 00:00:00