The application of comparative genomic hybridization and fluorescence in situ hybridization to the characterization of genotoxicity screening tester strains AHH-1 and MCL-5.

abstract：:Tobacco farming is an important economic income in Brazil, although it has been challenged as regard the occupational exposure to both pesticides and nicotine endured by farmers. Chronic occupational exposure to complex mixtures can lead to health hazardous. We examined genomic instability and epigenetic changes in to...

journal_title：Mutagenesis

authors： Kahl VFS,Dhillon VS,Simon D,da Silva FR,Salvador M,Branco CDS,Cappetta M,Martínez-López W,Thiesen FV,Dias JF,Souza CT,Fenech M,da Silva J

更新日期：2018-04-13 00:00:00

abstract：:Oxidative DNA lesions, constantly generated by both endogenous and environmentally induced reactive oxygen species, are removed via the base excision repair pathway. In bacteria, Fpg and Nei DNA glycosylases, belonging to the helix-two-turn-helix (H2TH) structural superfamily, remove oxidised purines and pyrimidines, ...

journal_title：Mutagenesis

authors： Makasheva KA,Endutkin AV,Zharkov DO

更新日期：2020-02-13 00:00:00

abstract：:In the present study we evaluated campthotecin (CPT)-induced chromosomal damage in human lymphocytes in the G0 phase of the cell cycle as revealed by the premature chromosome condensation technique. The results obtained here indicate that CPT was able to induce chromosome fragments in the G0 phase of the cell cycle of...

journal_title：Mutagenesis

authors： Mosesso P,Fonti E,Bassi L,Lorenti Garcia C,Palitti F

更新日期：1999-01-01 00:00:00

abstract：:Chemical carcinogenesis can be induced by genotoxic (GTX) or non-genotoxic (NGTX) carcinogens. GTX carcinogens have a well-described mode of action. However, the complex mechanisms by which NGTX carcinogens act are less clear and may result in conflicting results between species [e.g. Wy-14,643 (Wy)]. We hypothesise t...

journal_title：Mutagenesis

authors： Rieswijk L,Brauers KJ,Coonen ML,van Breda SG,Jennen DG,Kleinjans JC

更新日期：2015-11-01 00:00:00

abstract：:Exposure to ambient air particles is associated with elevated levels of DNA strand breaks (SBs) and endonuclease III, formamidopyrimidine DNA glycosylase (FPG) and oxoguanine DNA glycosylase-sensitive sites in cell cultures, animals and humans. In both animals and cell cultures, increases in SB and in oxidatively dama...

journal_title：Mutagenesis

authors： Møller P,Hemmingsen JG,Jensen DM,Danielsen PH,Karottki DG,Jantzen K,Roursgaard M,Cao Y,Kermanizadeh A,Klingberg H,Christophersen DV,Hersoug LG,Loft S

更新日期：2015-01-01 00:00:00

abstract：:Among the tests used to determine the mutagenic potential of chemicals, the chromosomal aberrations and micronucleus assays play an important role. These tests score either chromosomal structural aberrations at metaphase or micronuclei at interphase. One of the hallmarks of apoptosis is DNA fragmentation into 50-300 k...

journal_title：Mutagenesis

authors： Meintières S,Biola A,Pallardy M,Marzin D

更新日期：2001-05-01 00:00:00

abstract：:Polychlorinated biphenyls (PCBs) are stable pollutants, which can be found in almost every compartment of terrestrial and aquatic ecosystems. They are very lipophilic and therefore have the potency of accumulating in the fat stores of animals. The mechanisms by which PCBs exert their adverse effects are still unclear....

journal_title：Mutagenesis

authors： Belpaeme K,Delbeke K,Zhu L,Kirsch-Volders M

更新日期：1996-09-01 00:00:00

abstract：:The use of a white-ivory (wi) strain of Drosophila melanogaster carrying four copies of this allele, (wi)4, has proved to be useful in detecting somatic mutation in genotoxicity testing. Nevertheless, until now very little information exists about the nature of the genetic effects detected in such a strain. This work ...

journal_title：Mutagenesis

authors： Suárez S,Cabré O,Velázquez A,Marcos R,Xamena N

更新日期：1999-03-01 00:00:00

abstract：:The toxicological relevance of the micronucleus (MN) test is well defined: it is a multi-target genotoxic endpoint, assessing not only clastogenic and aneugenic events but also some epigenetic effects, which is simple to score, accurate, applicable in different cell types. In addition, it is predictive for cancer, ame...

journal_title：Mutagenesis

authors： Kirsch-Volders M,Decordier I,Elhajouji A,Plas G,Aardema MJ,Fenech M

更新日期：2011-01-01 00:00:00

abstract：:The effect of cerium oxide nanoparticle (CeNP) in plants has elicited substantial controversy. While some investigators have reported that CeNP possesses antioxidant properties, others observed CeNP to induce reactive oxygen species (ROS). In spite of considerable research carried out on the effects of CeNP in metazoa...

journal_title：Mutagenesis

authors： Sadhu A,Ghosh I,Moriyasu Y,Mukherjee A,Bandyopadhyay M

更新日期：2018-04-13 00:00:00

abstract：:In order to establish the capacity of Mytilus spp. to form genotoxin-DNA adducts, a series of in vitro and in vivo studies were conducted in which tissue samples and animals were exposed to five model genotoxins. Following the in vitro characterization of the major adducts induced by the compounds, a series of in vivo...

journal_title：Mutagenesis

authors： Harvey JS,Parry JM

更新日期：1997-05-01 00:00:00

abstract：:There are several reports demonstrating that aneugens may preferentially affect segregation of particular chromosomes in somatic cells. Much less is known on specific susceptibility of individual chromosomes to non-disjunction in mammalian meiosis in response to chemical exposures. To explore possible chromosome-speci...

journal_title：Mutagenesis

authors： Sun F,Yin H,Eichenlaub-Ritter U

更新日期：2001-09-01 00:00:00

abstract：:Well-established in vitro methods for testing the genotoxic potency of chemicals--such as the Ames/Salmonella test, the mouse lymphoma assay, the micronucleus test and the chromosomal aberration test--show a high false-positive rate for predicting in vivo genotoxicity and carcinogenicity. Thus, there is a need for mor...

journal_title：Mutagenesis

authors： Mathijs K,Brauers KJ,Jennen DG,Lizarraga D,Kleinjans JC,van Delft JH

更新日期：2010-11-01 00:00:00

abstract：:Nitropyrenes are a class of polycyclic hydrocarbons that have been shown to be potent point mutagens in the Salmonella/mammalian microsome assay. However, relatively few data have been published on their ability to induce chromosome aberrations. In this paper we report the cytogenetic activity of 1-nitropyrene, 1-nitr...

journal_title：Mutagenesis

authors： Lafi A,Parry JM

更新日期：1987-01-01 00:00:00

abstract：:A transgenic mouse strain with a high copy number of rescuable lacZ sequences was evaluated for its effectiveness in detecting lacZ- mutations in selected tissues. Procarbazine, cyclophosphamide, ethylnitrosourea, 7,12-dimethylbenz[a]anthracene (DMBA), acrylamide and chlorambucil were tested following either single or...

journal_title：Mutagenesis

authors： Hoorn AJ,Custer LL,Myhr BC,Brusick D,Gossen J,Vijg J

更新日期：1993-01-01 00:00:00

abstract：:As part of the third UKEMS collaborative trial, ICI Central Toxicology Laboratory (CTL) tested the three study chemicals ethylmethanesulphonate (EMS), benzo[a]pyrene (B[a]P) and benzidine (BZD) in a microwell adaptation of the L5178Y gene mutation assay using the thymidine kinase (tk) locus. Cofactors for metabolic ac...

journal_title：Mutagenesis

authors： Clay P,Cross MF

更新日期：1990-01-01 00:00:00

abstract：:The mechanistic/mammalian target of rapamycin (mTOR) is a conserved protein kinase that controls several anabolic processes required for cell growth and proliferation. As such, mTOR has been implicated in an increasing number of pathological conditions, including cancer, obesity, type 2 diabetes and neurodegeneration....

journal_title：Mutagenesis

authors： Cargnello M,Tcherkezian J,Roux PP

更新日期：2015-03-01 00:00:00

abstract：:Monitoring oxidative stress in vivo is made easier by the ability to use samples obtained non-invasively, such as urine. The analysis of DNA oxidation, by measurement of oxidized 2'-deoxyribonucleosides in urine, particularly 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), has been reported extensively in the literatur...

journal_title：Mutagenesis

authors： Evans MD,Saparbaev M,Cooke MS

更新日期：2010-09-01 00:00:00

abstract：:Recombinant lambda phage DNA containing segments of human O6-methylguanine-DNA transferase gene was employed to localize this gene among the human chromosomes using non-radioactive in situ hybridization technique. This gene was found to be present at the telomeric end, 26q, of the long arm of the chromosome 10. ...

journal_title：Mutagenesis

authors： Natarajan AT,Vermeulen S,Darroudi F,Valentine MB,Brent TP,Mitra S,Tano K

更新日期：1992-01-01 00:00:00

abstract：:Hyperbaric oxygen (HBO) treatment as used therapeutically has been shown to induce DNA damage in the alkaline comet assay with leukocytes from test subjects. Using formamidopyrimidine-DNA glycosylase, a DNA repair enzyme which specifically nicks DNA at sites of 8-oxoguanines and formamidopyrimidines, we have detected ...

journal_title：Mutagenesis

authors： Speit G,Dennog C,Lampl L

更新日期：1998-01-01 00:00:00

abstract：:Three short-term assays (the SOS chromotest, the Ames-fluctuation test and the newt micronucleus test) were carried out to evaluate the genotoxicity of four trihalomethanes (chloroform, bromodichloromethane, chlorodibromomethane and bromoform). With the SOS chromotest, all the chemicals studied except chloroform were ...

journal_title：Mutagenesis

authors： Le Curieux F,Gauthier L,Erb F,Marzin D

更新日期：1995-07-01 00:00:00

abstract：:A modified method for the 32P-postlabeling assay that permits standardized quantitation for specific polycyclic aromatic hydrocarbon (PAH)-DNA adducts is described. This method has been designed to test the components of the 32P-postlabeling assay for use in the chemically specific detection of individual adducts with...

journal_title：Mutagenesis

authors： Shields PG,Harris CC,Petruzzelli S,Bowman ED,Weston A

更新日期：1993-03-01 00:00:00

abstract：:Genotoxic stress causes a reduced stability of the plant genome and has a detrimental effect on plant growth and productivity. Double-strand breaks (DSBs) are the most harmful of all DNA lesions because they cause the loss of genetic information on both strands of the DNA helix. In the presented study the coding and g...

journal_title：Mutagenesis

authors： Stolarek M,Gruszka D,Braszewska-Zalewska A,Maluszynski M

更新日期：2015-11-01 00:00:00

abstract：:We have investigated the genotoxicity of lambda-cyhalothrin (LCT) (CAS registry No. 91465-08-06), a pyrethroid insecticide, in bone marrow cells and in colonic crypt epithelial cells of groups of four rats per dose treated in vivo by gavage at doses of 0.8, 3.06 and 6.12 mg/kg body weight (body wt). We measured genoto...

journal_title：Mutagenesis

authors： Celik A,Mazmanci B,Camlica Y,Askin A,Cömelekoglu U

更新日期：2005-03-01 00:00:00

abstract：:The antineoplastic drug etoposide (ET) inhibits topoisomerase II (topo II) activity by forming a ternary complex (DNA-ET-topo II). This complex prevents the DNA-strand-rejoining activity of topo II and may result in structural chromosome aberrations. Inhibition of topo II activity may also predispose cells to aneuploi...

journal_title：Mutagenesis

authors： Mailhes JB,Marchetti F,Young D,London SN

更新日期：1996-07-01 00:00:00

abstract：:Chinese hamster ovary cells were exposed to N-hydroxy-2-aminofluorene, N-hydroxy-N'-acetylbenzidine and 1-nitrosopyrene, and the resulting DNA adducts, sister-chromatid exchanges (SCEs) and mutations at the hypoxanthine-guanine phosphoribosyl transferase locus were quantified. Each agent produced a major DNA adduct su...

journal_title：Mutagenesis

authors： Heflich RH,Morris SM,Beranek DT,McGarrity LJ,Chen JJ,Beland FA

更新日期：1986-05-01 00:00:00

abstract：:Vitamins with antioxidant properties have the ability to act as pro-oxidants, inducing oxidative damage and oxidative stress as opposed to preventing it. While vitamin supplements are commonly consumed, the scientific evidence for their health beneficial effects is inconclusive. In fact, even harmful effects have been...

journal_title：Mutagenesis

authors： Bergström T,Ersson C,Bergman J,Möller L

更新日期：2012-07-01 00:00:00

abstract：:In this study, we determined the induction and time-dependent accumulation of micronuclei in the peripheral blood of transgenic C57BL/6 p53+/- mice (p53+/- mice), FVB/N Tg.AC v-Ha-ras mice (Tg.AC mice) and their isogenic parental strains, FVB/N and C57BL/6 following inhalation exposure to benzene. Our objective was to...

journal_title：Mutagenesis

authors： Healy LN,Pluta LJ,James RA,Janszen DB,Torous D,French JE,Recio L

更新日期：2001-03-01 00:00:00

abstract：:Implementation of the seventh amendment to the EU Cosmetics Directive has driven much research into suitable in vitro alternative assays to support satisfactory risk assessments. One such assay is the reconstructed skin micronucleus (RSMN) assay. First reported in 2006, further development occurred and a standard prot...

journal_title：Mutagenesis

authors： Kidd D,Phillips S,Chirom T,Mason N,Smith R,Saul J,Whitwell J,Clements J

更新日期：2019-12-03 00:00:00

abstract：:Sanfetrinem cilexetil, a hexetil ester of a Trinem antibiotic, does not induce micronuclei in rat bone marrow cells or induce DNA repair synthesis in rat hepatocytes following oral dosing. However, in vitro chromosome damage and mutations are induced in mammalian cells lacking carboxylesterase activity (human lymphocy...

journal_title：Mutagenesis

authors： Oliver J,Naidoo A,Vandin L,Pugnaghi F,Gatehouse D,Comelli R

更新日期：2000-01-01 00:00:00