The detection of genotoxin-induced DNA adducts in the common mussel Mytilus edulis.

abstract：:The objective of the present study was to develop a cellular phenotype assay for nucleotide excision repair (NER), using benzo[a]pyrene diol epoxide (BPDE) as model mutagen. Since in vitro exposure to BPDE may lead to DNA strand breaks resulting from both direct interaction with DNA and incisions introduced by the rep...

journal_title：Mutagenesis

authors： Vande Loock K,Decordier I,Ciardelli R,Haumont D,Kirsch-Volders M

更新日期：2010-01-01 00:00:00

abstract：:An immunohistochemical method for the determination of 6-thioguanine resistant (TGr) lymphocytes in human blood samples has been developed. The new technique is a modification of the autoradiographic assay and uses labelling of DNA synthesizing cells by bromodeoxyuridine (BrdU) instead of tritiated thymidine. The labe...

journal_title：Mutagenesis

authors： Montero R,Norppa H,Autio K,Lindholm C,Ostrosky-Wegman P,Sorsa M

更新日期：1991-03-01 00:00:00

abstract：:Radiation-induced exchange aberrations are thought to arise as a consequence of misrejoining of free ends of DNA double strand breaks (dsbs). In quiescent mammalian cells this process of misrejoining is prevalently taken up by the non-homologous end joining (NHEJ) process. In order to investigate the role of glutathio...

journal_title：Mutagenesis

authors： Dutta A,Chakraborty A,Saha A,Ray S,Chatterjee A

更新日期：2005-09-01 00:00:00

abstract：:We have investigated the genotoxicity of lambda-cyhalothrin (LCT) (CAS registry No. 91465-08-06), a pyrethroid insecticide, in bone marrow cells and in colonic crypt epithelial cells of groups of four rats per dose treated in vivo by gavage at doses of 0.8, 3.06 and 6.12 mg/kg body weight (body wt). We measured genoto...

journal_title：Mutagenesis

authors： Celik A,Mazmanci B,Camlica Y,Askin A,Cömelekoglu U

更新日期：2005-03-01 00:00:00

abstract：:The non-syndromic cleft lip and/or palate (NSCL/P) is a common birth defect caused by a combination of genetic and environmental factors. The possible role of genome instability on NSCL/P patient needs more investigation, since DNA metabolism is an essential cellular function to keep cells with normal genotypes and ge...

journal_title：Mutagenesis

authors： Xavier LA,Bezerra JF,de Rezende AA,Oliveira RA,Dalmolin RJ,do Amaral VS

更新日期：2017-03-01 00:00:00

abstract：:Blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) is an autosomal dominant disorder characterized by blepharophimosis, ptosis and epicanthus inversus. Based on the presence and absence of premature ovarian failure, two clinical types have been distinguished. Both types of BPES have been mapped to chromosome ...

journal_title：Mutagenesis

authors： Tang S,Wang X,Lin L,Sun Y,Wang Y,Yu H

更新日期：2006-01-01 00:00:00

abstract：:3 beta-Hydroxy-13 alpha-amino-13,17-seco-5 alpha-androstan-17-oic-13,17-lactam-p-bis(2-chloroethyl) aminophenoxyacetate (NSC 294859) is a new modified steroidal alkylating agent. This compound was given by i.p. administration to mice bearing different types of tumour. It was found to exhibit good activity in L1210 and...

journal_title：Mutagenesis

authors： Tselepi MR,Demopoulos NA,Catsoulacos P

更新日期：1989-09-01 00:00:00

abstract：:This study investigated the effect of glucose and fructose, and advanced glycation end-products (AGEs) on genome damage in WIL2-NS cells, measured using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. The effect of AGEs was investigated using the bovine serum albumin (AGE-BSA) model system induced either w...

journal_title：Mutagenesis

authors： Deo P,McCullough CL,Almond T,Jaunay EL,Donnellan L,Dhillon VS,Fenech M

更新日期：2020-03-27 00:00:00

abstract：:The mechanism responsible for the modification of mutagenicity by chlorophyllin has been investigated using mutagenic compounds with different mechanisms of action, including the monofunctional alkylating agents, N-methyl-N'-nitrosourea (MNU) and ethylmethanesulphonate (EMS); nitrosamines related to tobacco products, ...

journal_title：Mutagenesis

authors： Romert L,Curvall M,Jenssen D

更新日期：1992-09-01 00:00:00

abstract：:AHH-1 TK+/- is a human B cell-derived lymphoblastoid cell line that constitutively expresses a high level of the cytochrome CYP1A1. The MCL-5 cell line was developed by transfection of AHH-1 with cDNAs encoding the human cytochrome P450s, CYP1A2, CYP2A6, CYP2E1, CYP3A4 and microsomal epoxide hydrolase carried in plasm...

journal_title：Mutagenesis

authors： Corso C,Parry EM

更新日期：1999-07-01 00:00:00

abstract：:Detailed dose-response data for two reference aneugens, vincristine and nocodazole, have been derived for the mouse bone marrow micronucleus assay. Positive data have also been described for six other micronucleus-inducing agents: cyclophosphamide; aniline; urethane; 1,2-dimethylhydrazine; 7,12-dimethylbenzanthracene ...

journal_title：Mutagenesis

authors： Tinwell H,Ashby J

更新日期：1991-05-01 00:00:00

abstract：:In response to DNA damage, cell cycle arrest, apoptosis, and DNA repair are mediated by a TP53 pathway that induces p21(WAF1/Cip1). The chemotherapeutic drug cis-diamminedichloroplatinum-II (cisplatin) damages cellular DNA by forming cis-diammineplatinum-N(7)-d[GpG] and cis-diammine-platinum-N(7)-d[ApG] adducts. To in...

journal_title：Mutagenesis

authors： van Gijssel HE,Leil TA,Weinberg WC,Divi RL,Olivero OA,Poirier MC

更新日期：2007-01-01 00:00:00

abstract：:A simplified method, using recombinant interleukin-2, foetal bovine serum and freeze-killed feeder cells, has been developed for the mass culture of T-lymphocytes derived from human peripheral blood. In this protocol, bulk cultures can be cryopreserved approximately 8 days after initiation, and subsequent mass culture...

journal_title：Mutagenesis

authors： O'Donovan MR,Freemantle MR,Hull G,Bell DA,Arlett CF,Cole J

更新日期：1995-05-01 00:00:00

abstract：:Three short-term assays (the SOS chromotest, the Ames-fluctuation test and the newt micronucleus test) were carried out to evaluate the genotoxicity of four trihalomethanes (chloroform, bromodichloromethane, chlorodibromomethane and bromoform). With the SOS chromotest, all the chemicals studied except chloroform were ...

journal_title：Mutagenesis

authors： Le Curieux F,Gauthier L,Erb F,Marzin D

更新日期：1995-07-01 00:00:00

abstract：:I suggest that there are several good reasons for retaining mammalian cell mutation assays in a basic test package for genotoxicity and that the possible existence of a non-clastogenic mammalian mutagen is not one of them. I put forward a number of propositions, not necessarily original, concerning in vitro assays, in...

journal_title：Mutagenesis

authors： Green MH

更新日期：1988-09-01 00:00:00

abstract：:The nucleoside analog zidovudine (3'-azido-3'-deoxythymidine, AZT), by itself or in combination with other anti- retroviral drugs, is used perinatally to prevent mother to child transmission of human immunodeficiency virus type 1. AZT is mutagenic in vitro and mutagenic and carcinogenic when administered to neonatal m...

journal_title：Mutagenesis

authors： Von Tungeln LS,Dobrovolsky VN,Bishop ME,Shaddock JG,Heflich RH,Beland FA

更新日期：2004-07-01 00:00:00

abstract：:Male infertility is a relevant social and medical problem. Male infertility is mostly caused by genetic disorders. The purpose of the study was to analyze the correlation of chromosome aberrations, as well as DNA fragmentation and various manifestations of spermatogenesis disorder. Sperm samples of 58 males with infer...

journal_title：Mutagenesis

authors： Alchinbayev MK,Aralbayeva AN,Tuleyeva LN,Duysenbayeva SM,Makazhanov MA

更新日期：2016-09-01 00:00:00

abstract：:Comparative investigations of sister-chromatid exchange (SCE) and micronucleus induction in the bone marrow of rats, mice and Chinese hamsters with the cytostatic alkylating mutagen cyclophosphamide (CP) revealed remarkable species differences in their mutagenic responses. With both test systems the sensitivities of t...

journal_title：Mutagenesis

authors： Madle E,Korte A,Beek B

更新日期：1986-11-01 00:00:00

abstract：:The presence of centromeric DNA was studied in micronuclei isolated from the blood of male ddY mice after five weekly intraperitoneal injections of mitomycin C (MMC), 1-beta-D-arabinofuranosylcytosine (Ara-C), colchicine (COL) or vinblastine sulfate (VBL). In agreement with our earlier findings, about half of the micr...

journal_title：Mutagenesis

authors： Mäki-Paakkanen J,Hayashi M,Suzuki T,Tanabe H,Honma M,Sofuni T

更新日期：1995-11-01 00:00:00

abstract：:Genetic damage in workers occupationally exposed to an antineoplastic drug was studied using the micronucleus (MN) test, the comet assay, the hprt gene mutation assay and the TCR gene mutation assay. The subjects were divided into two groups: (i) 21 workers from a plant producing methotrexate (MTX); (ii) 21 controls w...

journal_title：Mutagenesis

authors： Deng H,Zhang M,He J,Wu W,Jin L,Zheng W,Lou J,Wang B

更新日期：2005-09-01 00:00:00

abstract：:Isothiocyanates are plant-derived compounds that may be beneficial in the prevention of certain chronic diseases. Yet, by stimulating the production of reactive oxygen species (ROS), isothiocyanates can be genotoxic. Whether antioxidants influence isothiocyanate-induced genotoxicity is unclear, but this situation was ...

journal_title：Mutagenesis

authors： Hoffman JD,Ward WM,Loo G

更新日期：2015-05-01 00:00:00

abstract：:The genotoxic effects of benzene in lung cells of mice exposed to single acute doses by inhalation have been estimated by cytogenetic analysis of micronuclei in primary cultures of lung fibroblasts. Mice were nose-only exposed to 1000 p.p.m. for 30 or 60 min or to 3500 p.p.m. for 30 min and sacrificed 24 h after the e...

journal_title：Mutagenesis

authors： Ranaldi R,Bassani B,Villani P,Lombardi CC,Tanzarella C,Pacchierotti F

更新日期：1998-09-01 00:00:00

abstract：:Prostate cancer is a major health burden, being the second most commonly diagnosed malignancy in men worldwide. Overtreatment represents a major problem in prostate cancer therapy, leading to significant long-term quality-of-life effects for patients and a broad socio-ecological burden. Biomarkers that could facilitat...

journal_title：Mutagenesis

authors： Brezina S,Feigl M,Gumpenberger T,Staudinger R,Baierl A,Gsur A

更新日期：2020-07-11 00:00:00

abstract：:Nitro-polycyclic aromatic hydrocarbons (nitro-PAHs) are mutagenic and carcinogenic environmental pollutants found in diesel exhaust and on urban air pollution particles. In the present study, human bronchial epithelial BEAS-2B cells were exposed to 2-nitrobenzanthrone (2-NBA) and 3-nitrobenzanthrone (3-NBA). DNA damag...

journal_title：Mutagenesis

authors： Oya E,Ovrevik J,Arlt VM,Nagy E,Phillips DH,Holme JA

更新日期：2011-11-01 00:00:00

abstract：:Cyclooxygenase 2 (Cox-2, a rate-limiting enzyme in the conversion of arachidonic acid to prostanoids) has been implicated in several physiological and pathological processes, and it has been reported that polymorphisms in the regulatory region of Cox-2 might influence its expression, contributing to the interindividua...

journal_title：Mutagenesis

authors： Tang Z,Nie ZL,Pan Y,Zhang L,Gao L,Zhang Q,Qu L,He B,Song G,Zhang Y,Shukui Wang

更新日期：2011-11-01 00:00:00

abstract：:The International Agency for Research on Cancer has classified several antineoplastic drugs in Group 1 (human carcinogens), among which chlorambucil, cyclophosphamide (CP) and tamoxifen, Group 2A (probable human carcinogens), among which cisplatin, etoposide, N-ethyl- and N-methyl-N-nitrosourea, and Group 2B (possible...

journal_title：Mutagenesis

authors： Villarini M,Dominici L,Piccinini R,Fatigoni C,Ambrogi M,Curti G,Morucci P,Muzi G,Monarca S,Moretti M

更新日期：2011-05-01 00:00:00

abstract：:Fanconi anaemia (FA) is a rare autosomal recessive disease characterized by increased spontaneous and DNA crosslinker-induced chromosome instability, progressive pancytopenia and cancer susceptibility. An increasing number of genes are involved in FA, including the breast cancer susceptibility gene BRCA2. Five of the ...

journal_title：Mutagenesis

authors： Bogliolo M,Cabré O,Callén E,Castillo V,Creus A,Marcos R,Surrallés J

更新日期：2002-11-01 00:00:00

abstract：:Cyclophosphamide (CP)-induced micronuclei were evaluated in females of two strains of mice (C57BL and CD1), in F1 hybrid females and in fetuses (day 13 of gestation) obtained from different crosses. F1 adult hybrids from a cross between the strain with a high level of induced micronuclei (C57BL) and the strain with a ...

journal_title：Mutagenesis

authors： Barale R,Migliore L,Zucconi D,Ciranni R,Casini D,Carducci A,Loprieno N

更新日期：1987-05-01 00:00:00

abstract：:Despite their prime candidate status, polymorphisms near genes involved in DNA repair or in other functions related to genome stability have been conspicuously under-represented in the significant associations reported from genome-wide association studies (GWAS) of cancer susceptibility. In this study, we assessed a s...

journal_title：Mutagenesis

authors： Tomlinson IP,Houlston RS,Montgomery GW,Sieber OM,Dunlop MG

更新日期：2012-03-01 00:00:00

abstract：:O6-Methylguanine-DNA methyltransferase (MT) specific activity (fmol/mg protein) was measured in human T lymphocytes which were maintained as exponentially growing cultures from 6 to 17 days. The T lymphocytes were not transformed and were grown under the same conditions used previously for determination of spontaneous...

journal_title：Mutagenesis

authors： Bartlett JD,Robison SH

更新日期：1990-03-01 00:00:00