Abstract:
:Nitropyrenes are a class of polycyclic hydrocarbons that have been shown to be potent point mutagens in the Salmonella/mammalian microsome assay. However, relatively few data have been published on their ability to induce chromosome aberrations. In this paper we report the cytogenetic activity of 1-nitropyrene, 1-nitrosopyrene, and 1-aminopyrene in cultured Chinese hamster cells (Don:Wg3h). Following treatment with 1-nitrosopyrene, high levels of both chromosome and chromatid types of aberrations were induced, the most notable effect being an increase in chromatid exchanges. Much weaker, but statistically significant, responses were also obtained for 1-nitropyrene and 1-aminopyrene.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Lafi A,Parry JMdoi
10.1093/mutage/2.1.23subject
Has Abstractpub_date
1987-01-01 00:00:00pages
23-6issue
1eissn
0267-8357issn
1464-3804journal_volume
2pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::Fanconi anaemia (FA) is a rare autosomal recessive disease characterized by increased spontaneous and DNA crosslinker-induced chromosome instability, progressive pancytopenia and cancer susceptibility. An increasing number of genes are involved in FA, including the breast cancer susceptibility gene BRCA2. Five of the ...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/17.6.529
更新日期:2002-11-01 00:00:00
abstract::Antiretroviral therapy for the human immunodeficiency virus-1 (HIV-1) typically includes two nucleoside reverse transcriptase inhibitors (NRTIs). 3'-Azido-3'-deoxythymidine (AZT, Zidovudine) plus 2'-deoxy-3'-thiacytidine (3TC, Lamivudine) is a combination that is used frequently. The NRTIs are mutagenic nucleoside ana...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei019
更新日期:2005-03-01 00:00:00
abstract::Neurocysticercosis (NCC) has been associated with a high frequency of DNA damage in human circulating lymphocytes and more recently with the development of hematological malignancies. Chronic inflammation, a common feature of helminthic infections, has been proposed to play a key role in carcinogenesis induced by para...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/16.6.495
更新日期:2001-11-01 00:00:00
abstract::BRAF alterations represent a novel indicator of the progression and aggressiveness of thyroid carcinogenesis. So, the main aim of the study was to elucidate the involvement of BRAF gene mutations and its expression in Kashmiri (North India) patients and investigate their association with clinico-pathological character...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/get066
更新日期:2014-03-01 00:00:00
abstract::A modified protocol is described for the in vitro analysis of micronuclei in whole blood or separated lymphocyte cultures. The induction of binucleate cells by various concentrations of cytochalasin B (3, 4.5 or 6 micrograms/ml) was examined at two harvest times (68 or 72 h). An optimal yield was obtained by adding cy...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.4.317
更新日期:1993-07-01 00:00:00
abstract::This study investigated the effect of glucose and fructose, and advanced glycation end-products (AGEs) on genome damage in WIL2-NS cells, measured using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. The effect of AGEs was investigated using the bovine serum albumin (AGE-BSA) model system induced either w...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geaa002
更新日期:2020-03-27 00:00:00
abstract::Mutation induction by UV irradiation was studied in a retroviral vector integrated in one copy per cell at various chromosomal positions. As a mutational target, hamster hprt cDNA was present on the retroviral vector. To minimize the influence of repair we used repair-deficient hamster cells, V-H1 and UV5, as a recipi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.5.399
更新日期:1993-09-01 00:00:00
abstract::The accumulation of mitochondrial DNA (mtDNA) mutations has been proposed as an underlying cause of the ageing process and mutations have been associated with cancer in many tissues, including human skin. This involvement is linked to the key roles of mitochondrial function and mtDNA in oxidative stress production and...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/gep061
更新日期:2010-03-01 00:00:00
abstract::Potential toxicological interactions of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and/or dibutyl phthalate (DBP) with ozone were investigated. Male and female B6C3F1 mice were exposed to ozone (0.5 p.p.m.), NNK (1.0 mg/kg), DBP (5000 p.p.m.) and different combinations of these toxicants 6 h/day for 16,...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/17.4.331
更新日期:2002-07-01 00:00:00
abstract::Radiation-induced exchange aberrations are thought to arise as a consequence of misrejoining of free ends of DNA double strand breaks (dsbs). In quiescent mammalian cells this process of misrejoining is prevalently taken up by the non-homologous end joining (NHEJ) process. In order to investigate the role of glutathio...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei046
更新日期:2005-09-01 00:00:00
abstract::The aim of the present work was to investigate the possible interference of cytochalasin B (cyt B) with low concentration treatment with colchicine in the induction of chromosome/chromatid loss and micronuclei in human lymphocytes mitotically activated in vitro. Thus, cells from a single female donor were treated with...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.1.43
更新日期:1999-01-01 00:00:00
abstract::Non-homologous end joining (NHEJ), the major pathway of double-strand break (DSB) repair in mammalian cells, comprises two subpathways: one that requires the three core factors Ku70/80, DNA-PKcs and XRCC4/LigIV (DNA-PK-dependent NHEJ) and the other that is independent of these factors. Using a cell-free NHEJ assay, we...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gem007
更新日期:2007-05-01 00:00:00
abstract::Deregulation of control of the apoptotic process in Fanconi anaemia (FA) appears to be one of the main features of this disease at the cellular level. We show here that FA cells are resistant to treatments with rhodamine-1,2,3 and doxycycline, which both interfere with mitochondrial functionality by different mechanis...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/17.1.25
更新日期:2002-01-01 00:00:00
abstract::Detailed dose-response data for two reference aneugens, vincristine and nocodazole, have been derived for the mouse bone marrow micronucleus assay. Positive data have also been described for six other micronucleus-inducing agents: cyclophosphamide; aniline; urethane; 1,2-dimethylhydrazine; 7,12-dimethylbenzanthracene ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/6.3.193
更新日期:1991-05-01 00:00:00
abstract::Radiation-induced bystander effects have been evaluated extensively, including the involvement of the mitogen-activated protein kinase (MAPK) pathways. However, few studies have examined the ability of chemicals to induce bystander effects, and the molecular mechanisms involved in chemical bystander effects have not b...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq003
更新日期:2010-05-01 00:00:00
abstract::The aim of the present work was to investigate the processes involved in the origin of trisomic karyotypes, i.e. co-migration of sister chromatids (mitotic non-disjunction, MND) and recovery of micronuclei (MN) originating from lagging chromosomes/chromatids at anaphase (mitotic indirect non-disjunction, MIND), and to...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.6.557
更新日期:1999-11-01 00:00:00
abstract::A perceived disadvantage of transgenic rodent mutation assays is that spontaneous mutant frequencies are high compared to those of endogenous genes and may consequently reduce sensitivity to induced mutation. We have previously argued that unrepaired G:T mismatches from spontaneous deamination of 5-methylcytosine at C...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen026
更新日期:2008-09-01 00:00:00
abstract::Activation of the human bladder carcinogen 4-aminobiphenyl (ABP) and its N-hydroxy derivatives was investigated using lysates and subcellular enzyme preparations from cultured human uroepithelial cells (HUC). Mutagenic activation was determined using Salmonella typhimurium strains TA98; TA98/1,8-DNP6, a derivative def...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.2.113
更新日期:1993-03-01 00:00:00
abstract::Prostate cancer is a major health burden, being the second most commonly diagnosed malignancy in men worldwide. Overtreatment represents a major problem in prostate cancer therapy, leading to significant long-term quality-of-life effects for patients and a broad socio-ecological burden. Biomarkers that could facilitat...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geaa010
更新日期:2020-07-11 00:00:00
abstract::This is an investigation of the long-term effects of a shift from a mixed diet to a lacto-vegetarian diet and of a return to a mixed diet on the mutagenic activity in urine and faeces. The participants were 20 normal weight, non-smoking subjects. Dietary surveys and urinary and faecal samples were collected before and...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.2.167
更新日期:1998-03-01 00:00:00
abstract::A modified method for the 32P-postlabeling assay that permits standardized quantitation for specific polycyclic aromatic hydrocarbon (PAH)-DNA adducts is described. This method has been designed to test the components of the 32P-postlabeling assay for use in the chemically specific detection of individual adducts with...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.2.121
更新日期:1993-03-01 00:00:00
abstract::The induction of aneuploidy in cultured mammalian cells has been studied using two chemical agents, diethylstilboestrol dipropionate and Colcemid, which act on the mitotic spindle, in order to investigate different methods of scoring and statistical analysis. Two cell lines, one human and the other Chinese hamster, we...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.5.391
更新日期:1987-09-01 00:00:00
abstract::Chromatographic profiles of DNA adducts formed following treatment of Chinese hamster ovary (CHO) cells or C3H10T1/2 mouse embryo fibroblast cells with benzo[a]pyrene (BP), in the presence or absence of rat liver S9 fraction, have been obtained by both direct labelling ([3H]BP) and 32P-post-labelling methods. The prin...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/5.4.381
更新日期:1990-07-01 00:00:00
abstract::The clastogenic potential of sodium fluoride was determined both in vitro (using cultured human lymphocytes) and in vivo (using the rat bone-marrow micronucleus test). The incidence of chromosome aberrations in human lymphocyte cultures exposed to 20 or 40 micrograms/ml sodium fluoride (3 and 9% respectively) was sign...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.6.497
更新日期:1987-11-01 00:00:00
abstract::In order to validate the in vivo micronucleus test in mouse splenocytes using the cytokinesis block method, 14 compounds with various mechanisms of action were tested: three direct alkylating agents (mitomycin C, ethylnitrosourea, beta-propiolactone), seven indirect alkylating agents (cyclophosphamide, benzo[a]pyrene,...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/9.3.199
更新日期:1994-05-01 00:00:00
abstract::Coal miners are exposed to coal dust, containing mineral particles, inorganic compounds and polycyclic aromatic hydrocarbons, and to ionizing radiation. These factors can induce oxidative stress and promote inflammation that leads to DNA damage. The aim of this investigation is to analyse the degree of DNA damage in m...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew038
更新日期:2016-11-01 00:00:00
abstract::Urine and serum samples of rats bearing three different experimental tumours (hepatocellular carcinoma, myelomonocytic leukemia and mesoblastic nephroma) were investigated for mutagenicity with the Ames Salmonella test. Enhancement of mutagenic activity in TA98 and TA100 was observed only in the case of urine samples ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/10.1.43
更新日期:1995-01-01 00:00:00
abstract::This paper presents the results obtained within the framework of an EU research project aimed at investigating the relationship between occupational exposure to pesticides and the induction of cytogenetic damage. Populations from Greece, Spain, Poland and Hungary, all of them characterised by intensive agricultural ac...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/18.3.249
更新日期:2003-05-01 00:00:00
abstract::The antineoplastic drug etoposide (ET) inhibits topoisomerase II (topo II) activity by forming a ternary complex (DNA-ET-topo II). This complex prevents the DNA-strand-rejoining activity of topo II and may result in structural chromosome aberrations. Inhibition of topo II activity may also predispose cells to aneuploi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.4.357
更新日期:1996-07-01 00:00:00
abstract::In Saccharomyces cerevisiae, disruption of genes by deletion allowed elucidation of the molecular mechanisms of a series of human diseases, such as in Wilson disease (WD). WD is a disorder of copper metabolism, due to inherited mutations in human copper-transporting ATPase (ATP7B). An orthologous gene is present in S....
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gev062
更新日期:2016-01-01 00:00:00