Abstract:
:Mutation induction by UV irradiation was studied in a retroviral vector integrated in one copy per cell at various chromosomal positions. As a mutational target, hamster hprt cDNA was present on the retroviral vector. To minimize the influence of repair we used repair-deficient hamster cells, V-H1 and UV5, as a recipient for the vector. There is no major influence of chromosomal position on UV-induced mutation frequency and spectrum because no statistically significant difference between mutation induction in retroviral cDNA copies integrated at different chromosomal sites was observed. However, a major difference was found in mutation induction between the endogenous hamster hprt gene and the retroviral cDNA copies. Most noticeable was the absence in the cDNA of the strong strand bias for mutation induction, which was reported for the endogenous hprt gene. Our results with the hprt cDNA exclude as a general phenomenon a difference in mutation induction for leading and lagging strand DNA replication, which was proposed as an explanation for this strand bias in the endogenous gene. The similarity of mutation induction in the different retroviral cDNA copies, all directly surrounded by the same DNA sequence elements, together with the marked difference between the mutation induction in the endogenous gene and the cDNA copies may point to an important role of chromatin structure in mutation induction.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Tasseron-de Jong JG,den Dulk H,Lichtenauer-Kaligis EG,Kroone RC,Giphart-Gassler M,van de Putte Pdoi
10.1093/mutage/8.5.399subject
Has Abstractpub_date
1993-09-01 00:00:00pages
399-406issue
5eissn
0267-8357issn
1464-3804journal_volume
8pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::The repairability during G(1) of DNA lesions eliciting sister chromatid exchanges (SCE) induced by methylmethanesulfonate (MMS) and ethylmethanesulfonate (EMS) in BrdU-substituted and unsubstituted DNA strands was determined in murine salivary gland cells in vivo. The SCE frequency was determined after exposure to MMS...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/18.1.13
更新日期:2003-01-01 00:00:00
abstract::Carbon nanotubes (CNTs) are fibrous carbon-based nanomaterials with a potential to cause carcinogenesis in humans. Alterations in DNA methylation on cytosine-phosphate-guanidine (CpG) sites are potential markers of exposure-induced carcinogenesis. This study examined cytotoxicity, genotoxicity and DNA methylation alte...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew053
更新日期:2017-01-01 00:00:00
abstract::This paper presents the results obtained within the framework of an EU research project aimed at investigating the relationship between occupational exposure to pesticides and the induction of cytogenetic damage. Populations from Greece, Spain, Poland and Hungary, all of them characterised by intensive agricultural ac...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/18.3.249
更新日期:2003-05-01 00:00:00
abstract::Fanconi anaemia (FA) patients show cellular sensitivity to a variety of clastogens and prominently to cross-linking agents. Although there is a long-standing clinical impression of radiosensitivity, in vitro studies have yielded conflicting results. In this study, initial radiation-induced DNA damage and kinetics of D...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen052
更新日期:2009-01-01 00:00:00
abstract::Ionising radiation induces several isolated and clustered DNA lesions in human cells. Depending on the type of lesions, DNA repair pathways get activated to maintain the integrity of the genome. Base excision repair (BER) pathway is known to repair single-strand breaks and base damages through short- and long-patch ge...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geu065
更新日期:2015-03-01 00:00:00
abstract::In a sample of healthy subjects (23-59 years old), comprising 49 non-smokers and 23 smokers, we studied the effects of smoking on DNA repair synthesis (UDS) in peripheral lymphocytes. DNA repair after UV-irradiation was measured by [3H]TdR incorporation into DNA (semi-conservative synthesis was inhibited by hydroxyure...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.2.82
更新日期:1989-03-01 00:00:00
abstract::Coleus aromaticus (Benth, Family: Laminaceae), Indian Oregano native to India and Mediterranean, is well known for its medicinal properties. A preliminary study was undertaken to elucidate in vitro free radical scavenging potential and inhibition of lipid peroxidation by C.aromaticus hydroalcoholic extract (CAE). Anti...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gel023
更新日期:2006-07-01 00:00:00
abstract::An established human hepatoma cell strain (Hep G2) was used in micronuclei (MN) and sister chromatid exchange (SCE) assays to evaluate the clastogenic potential of several indirectly-acting mutagenic carcinogens. Benzo[a]pyrene, cyclophosphamide, dimethyl nitrosamine, hexamethylphosphoramide, pyrene and safrole were s...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/6.5.399
更新日期:1991-09-01 00:00:00
abstract::Induced genomic instability in the human B lymphoblastoid cell line TK6 manifests itself as increases in end-to-end chromosome fusions and non-reciprocal chromosome translocations. It is not associated with elevated frequencies of specific locus mutations or other cytogenetic alterations. Previous studies on a limited...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geh060
更新日期:2004-11-01 00:00:00
abstract::We have investigated the relative importance of DNA methylation-directed repair of replication errors to the production of mutation/gene rearrangements in mammalian cells with hypomethylated genomes. Using a series of sibling (Chinese hamster derived) cell clones with stably altered DNA methylation levels, we found th...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.6.462
更新日期:1989-11-01 00:00:00
abstract::Chinese hamster ovary cells were exposed to N-hydroxy-2-aminofluorene, N-hydroxy-N'-acetylbenzidine and 1-nitrosopyrene, and the resulting DNA adducts, sister-chromatid exchanges (SCEs) and mutations at the hypoxanthine-guanine phosphoribosyl transferase locus were quantified. Each agent produced a major DNA adduct su...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/1.3.201
更新日期:1986-05-01 00:00:00
abstract::Transgenic B6C3F1 and C57BL/6 mice containing a lambda shuttle vector that carries a lacI target and an alpha lacZ reporter gene have been constructed for use in in vivo mutagenesis assays. After chemical treatment of mice carrying the lacI target gene, genomic DNA is isolated and the shuttle vector is recovered by ex...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.3.265
更新日期:1993-05-01 00:00:00
abstract::Radiation-induced exchange aberrations are thought to arise as a consequence of misrejoining of free ends of DNA double strand breaks (dsbs). In quiescent mammalian cells this process of misrejoining is prevalently taken up by the non-homologous end joining (NHEJ) process. In order to investigate the role of glutathio...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei046
更新日期:2005-09-01 00:00:00
abstract::A transgenic mouse strain with a high copy number of rescuable lacZ sequences was evaluated for its effectiveness in detecting lacZ- mutations in selected tissues. Procarbazine, cyclophosphamide, ethylnitrosourea, 7,12-dimethylbenz[a]anthracene (DMBA), acrylamide and chlorambucil were tested following either single or...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.1.7
更新日期:1993-01-01 00:00:00
abstract::Antiretroviral therapy for the human immunodeficiency virus-1 (HIV-1) typically includes two nucleoside reverse transcriptase inhibitors (NRTIs). 3'-Azido-3'-deoxythymidine (AZT, Zidovudine) plus 2'-deoxy-3'-thiacytidine (3TC, Lamivudine) is a combination that is used frequently. The NRTIs are mutagenic nucleoside ana...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei019
更新日期:2005-03-01 00:00:00
abstract::Autosomal recessive woolly hair/hypotrichosis (ARWH/HT: OMIM #278150/604379) is a rare hereditary hair disease characterized by tightly curled hair at birth which can lead to sparse hair later in life. The mutations in both LIPH and LPAR6/P2RY5 are responsible for autosomal recessive woolly hair with or without hypotr...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gex043
更新日期:2017-12-31 00:00:00
abstract::The antineoplastic drug etoposide (ET) inhibits topoisomerase II (topo II) activity by forming a ternary complex (DNA-ET-topo II). This complex prevents the DNA-strand-rejoining activity of topo II and may result in structural chromosome aberrations. Inhibition of topo II activity may also predispose cells to aneuploi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.4.357
更新日期:1996-07-01 00:00:00
abstract::Inter-individual differences in susceptibility to breast cancer are partially mediated through the levels of endogenous and exogenous steroid hormones. The CYP17 gene encodes P450c17alpha, an enzyme that is involved in the metabolism of steroid hormones. Increased endogenous steroid hormone levels have been associated...
journal_title:Mutagenesis
pub_type: 杂志文章,meta分析
doi:10.1093/mutage/17.2.119
更新日期:2002-03-01 00:00:00
abstract::Many chronic inflammatory conditions are associated with an increased risk of cancer development. At the site of inflammation, cellular DNA is damaged by hypochlorous acid (HOCl), a potent oxidant generated by myeloperoxidase. 8-Chloro-2'-deoxyguanosine (8-Cl-dG) is a major DNA adduct formed by HOCl and has been detec...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ges056
更新日期:2013-01-01 00:00:00
abstract::Previous reports showed the protective effect of the synthetic antioxidant butylated hydroxytoluene (BHT) against the chromosomal damage induced by bleomycin (BLM), cadmium chloride and potassium dichromate. To test the hypothesis that this effect was exerted by inhibition and/or scavenging of reactive oxygen species ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gel046
更新日期:2006-11-01 00:00:00
abstract::The aim of the present work was to investigate the possible interference of cytochalasin B (cyt B) with low concentration treatment with colchicine in the induction of chromosome/chromatid loss and micronuclei in human lymphocytes mitotically activated in vitro. Thus, cells from a single female donor were treated with...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.1.43
更新日期:1999-01-01 00:00:00
abstract::Niclosamide produces genotoxic effects, such as point mutations in Salmonella sp., sperm-head abnormalities in mice and clastogenic effects in human lymphocytes in vitro and in vivo. As cytochrome P450 could be involved in the bioactivation of niclosamide, we investigated which subfamily was involved. We used liver mi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/get043
更新日期:2013-11-01 00:00:00
abstract::The effects of salts of 27 elements on recombination and on the production of disomic and/or diploid spores during meiosis of Saccharomyces cerevisiae has been investigated. Be(NO3)2, MgSO4, FeSO4, CuSO4, AgNO3, Na2HAsO4 were inactive on the events studied during meiotic cell division. AuCl4, CdCl2, C4H6O4Pb, SnCl2, K...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/1.1.21
更新日期:1986-01-01 00:00:00
abstract::Type 2 diabetes mellitus has undergone a worldwide growth in incidence in the world and has now acquired epidemic status. There is a strong link between type 2 diabetes and vitamin D deficiency. Because vitamin D has beneficial effects on glucose homeostasis, the aim of this study was to evaluate the influence of vita...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gez001
更新日期:2019-05-29 00:00:00
abstract::Implementation of the seventh amendment to the EU Cosmetics Directive has driven much research into suitable in vitro alternative assays to support satisfactory risk assessments. One such assay is the reconstructed skin micronucleus (RSMN) assay. First reported in 2006, further development occurred and a standard prot...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gez037
更新日期:2019-12-03 00:00:00
abstract::CASE, an artificial intelligence structure-activity relational system, was used to predict the carcinogenicity of a group of chemicals currently being tested in rodent bioassays by the US National Toxicology Program. The 'learning set' for the CASE predictions consisted of the results of previous 252 rodent carcinogen...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/5.5.425
更新日期:1990-09-01 00:00:00
abstract::As part of the third UKEMS collaborative trial, ICI Central Toxicology Laboratory (CTL) tested the three study chemicals ethylmethanesulphonate (EMS), benzo[a]pyrene (B[a]P) and benzidine (BZD) in a microwell adaptation of the L5178Y gene mutation assay using the thymidine kinase (tk) locus. Cofactors for metabolic ac...
journal_title:Mutagenesis
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:1990-01-01 00:00:00
abstract::The present study was undertaken to compare the antimutagenic activity of aqueous extracts, at the concentrations used for human consumption, from green, black and decaffeinated black tea. Antimutagenic potential was evaluated against three indirect-acting dietary carcinogens, Glu-P-1, benzo(a)pyrene and nitrosopyrrol...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/11.6.597
更新日期:1996-11-01 00:00:00
abstract::BRAF alterations represent a novel indicator of the progression and aggressiveness of thyroid carcinogenesis. So, the main aim of the study was to elucidate the involvement of BRAF gene mutations and its expression in Kashmiri (North India) patients and investigate their association with clinico-pathological character...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/get066
更新日期:2014-03-01 00:00:00
abstract::Many chemical carcinogens require metabolic activation to form genotoxic compounds in human. Standard in vitro genotoxicity assays performed with activation systems, such as rat liver S9, are recognised to lead to a high number of false positives. The aim of this study was to evaluate the suitability of differentiated...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq039
更新日期:2010-11-01 00:00:00