Abstract:
:In order to validate the in vivo micronucleus test in mouse splenocytes using the cytokinesis block method, 14 compounds with various mechanisms of action were tested: three direct alkylating agents (mitomycin C, ethylnitrosourea, beta-propiolactone), seven indirect alkylating agents (cyclophosphamide, benzo[a]pyrene, diethylnitrosamine, dimethylnitrosamine, 4-aminophenol, 4-aminobiphenyl, 1,1-dimethylhydrazine), two intercalating agents (acridine orange, ethidium bromide) and two spindle poisons (vincristine, colchicine). Male mice were dosed once with the compound, and spleen samples were taken 2 or 14 days after treatment. A significant increase in the binucleated micronucleated splenocyte rate was observed with all the alkylating and intercalating agents at at least one sampling time. In contrast, no increase in the binucleated micronucleated splenocyte rate was observed with the spindle poisons. In conclusion, under these experimental conditions, this in vivo test seems appropriate for the detection of clastogenic compounds including compounds that cannot be detected in the bone marrow micronucleus test. The limit of this test, as expected, is the lack of detection of aneugenic compounds.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Benning V,Brault D,Duvinage C,Thybaud V,Melcion Cdoi
10.1093/mutage/9.3.199subject
Has Abstractpub_date
1994-05-01 00:00:00pages
199-204issue
3eissn
0267-8357issn
1464-3804journal_volume
9pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::The use of a white-ivory (wi) strain of Drosophila melanogaster carrying four copies of this allele, (wi)4, has proved to be useful in detecting somatic mutation in genotoxicity testing. Nevertheless, until now very little information exists about the nature of the genetic effects detected in such a strain. This work ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.2.187
更新日期:1999-03-01 00:00:00
abstract::The widespread production and use of nanoparticles calls for faster and more reliable methods to assess their safety. The main aim of this study was to investigate the genotoxicity of three reference TiO2 nanomaterials (NM) within the frame of the FP7-NANoREG project, with a particular focus on testing the applicabili...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew030
更新日期:2017-01-01 00:00:00
abstract::Comparative investigations of sister-chromatid exchange (SCE) and micronucleus induction in the bone marrow of rats, mice and Chinese hamsters with the cytostatic alkylating mutagen cyclophosphamide (CP) revealed remarkable species differences in their mutagenic responses. With both test systems the sensitivities of t...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/1.6.419
更新日期:1986-11-01 00:00:00
abstract::Radiation-induced exchange aberrations are thought to arise as a consequence of misrejoining of free ends of DNA double strand breaks (dsbs). In quiescent mammalian cells this process of misrejoining is prevalently taken up by the non-homologous end joining (NHEJ) process. In order to investigate the role of glutathio...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gei046
更新日期:2005-09-01 00:00:00
abstract::Studies in mono-culture of cells have shown that diesel exhaust particles (DEPs) increase the production of reactive oxygen species (ROS) and oxidative stress-related damage to DNA. However, the level of particle-generated genotoxicity may depend on interplay between different cell types, e.g. lung epithelium and immu...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ges035
更新日期:2012-11-01 00:00:00
abstract::Dibenz[a,h]anthracene (DB[a,h]A) is a polycyclic aromatic hydrocarbon that is a by-product of combustion and a potent carcinogen. Few studies have investigated the effects of DB[a,h]A on mRNA and microRNA expression to dissect the mechanisms involved in carcinogenesis. In this study, mature male mice (Muta(™)Mouse) we...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/get031
更新日期:2013-09-01 00:00:00
abstract::Macrocyclic polyethers (crown ethers) are a family of compounds which possess the ability to transport ionic species across natural and artificial membranes. Because of this characteristic, they have wide applications in industry and are being investigated for their potential as pharmacologic agents. However, these co...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.6.437
更新日期:1989-11-01 00:00:00
abstract::The mechanism responsible for the modification of mutagenicity by chlorophyllin has been investigated using mutagenic compounds with different mechanisms of action, including the monofunctional alkylating agents, N-methyl-N'-nitrosourea (MNU) and ethylmethanesulphonate (EMS); nitrosamines related to tobacco products, ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/7.5.349
更新日期:1992-09-01 00:00:00
abstract::Swiss albino mice were exposed to formulated cypermethrin (CMR) and/or or chlorpyrifos (CPF) through oral gavages for 60 days. Test doses of CMR (0.69, 1.38 or 2.76mg/kg/day) or CPF (0.5, 1.0 or 2.0mg/kg/day) or CMR + CPF (0.69 + 0.5, 1.38 + 1.0 or 2.76 + 2.0mg/kg/day) were based on the acute oral median lethal doses ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew031
更新日期:2016-11-01 00:00:00
abstract::Non-cytotoxic concentrations (1.5, 3 and 6 microM) of sodium arsenite (SA) were used to study its cytogenetic effects with special reference to the frequency and nature of chromosomal aberrations on the X chromosome at Xq21 and at centromeric regions in CHO9, EM-C11, V79, V-H4 and CHE cell lines using Giemsa and FISH ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.3.229
更新日期:1998-05-01 00:00:00
abstract::The non-syndromic cleft lip and/or palate (NSCL/P) is a common birth defect caused by a combination of genetic and environmental factors. The possible role of genome instability on NSCL/P patient needs more investigation, since DNA metabolism is an essential cellular function to keep cells with normal genotypes and ge...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew068
更新日期:2017-03-01 00:00:00
abstract::Gaulden reported a novel and unexpected mitomycin C (MMC) effect, namely a pronounced retardation of very late prophase and loss of chromosome orientation in neuroblasts of the grasshopper Chortophaga viridifasciate. Because this effect may be due to interactions of MMC with non-DNA targets, MMC was tested for its int...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.1.39
更新日期:1989-01-01 00:00:00
abstract::Many chemical carcinogens require metabolic activation to form genotoxic compounds in human. Standard in vitro genotoxicity assays performed with activation systems, such as rat liver S9, are recognised to lead to a high number of false positives. The aim of this study was to evaluate the suitability of differentiated...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq039
更新日期:2010-11-01 00:00:00
abstract::CASE, an artificial intelligence structure-activity relational system, was used to predict the carcinogenicity of a group of chemicals currently being tested in rodent bioassays by the US National Toxicology Program. The 'learning set' for the CASE predictions consisted of the results of previous 252 rodent carcinogen...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/5.5.425
更新日期:1990-09-01 00:00:00
abstract::Radiation-induced bystander effects have been evaluated extensively, including the involvement of the mitogen-activated protein kinase (MAPK) pathways. However, few studies have examined the ability of chemicals to induce bystander effects, and the molecular mechanisms involved in chemical bystander effects have not b...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq003
更新日期:2010-05-01 00:00:00
abstract::Coal miners are exposed to coal dust, containing mineral particles, inorganic compounds and polycyclic aromatic hydrocarbons, and to ionizing radiation. These factors can induce oxidative stress and promote inflammation that leads to DNA damage. The aim of this investigation is to analyse the degree of DNA damage in m...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gew038
更新日期:2016-11-01 00:00:00
abstract::The human metabolome-the complement of small molecule metabolites present in biofluids and tissues-represents a significant part of the internal chemical milieu and is therefore an important aspect of the human exposome. Metabolic profiling approaches, commonly referred to as metabonomics or metabolomics, permit detai...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/gev060
更新日期:2015-11-01 00:00:00
abstract::An established human hepatoma cell strain (Hep G2) was used in micronuclei (MN) and sister chromatid exchange (SCE) assays to evaluate the clastogenic potential of several indirectly-acting mutagenic carcinogens. Benzo[a]pyrene, cyclophosphamide, dimethyl nitrosamine, hexamethylphosphoramide, pyrene and safrole were s...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/6.5.399
更新日期:1991-09-01 00:00:00
abstract::Published data regarding the association between the apurinic/apyrimidinic endonuclease 1 (APE1) T1349G (Asp148Glu) polymorphism and cancer risk show inconclusive results. To derive a more precise estimation of the relationship, we performed a meta-analysis of 27 published studies that included 12 432 cancer cases and...
journal_title:Mutagenesis
pub_type: 杂志文章,meta分析
doi:10.1093/mutage/gep036
更新日期:2009-11-01 00:00:00
abstract::The complementation group (CG) assignment is presented for 74 moderately sensitive (approximately 2-4x sensitivity of parental line based on ratio of D10s) UV-sensitive mutants of Chinese hamster ovary (CHO) cells from the Facility for Automated Experiments in Cell Biology (FAECB) collection. The distribution of mutan...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/9.4.301
更新日期:1994-07-01 00:00:00
abstract::The importance of diet in DNA damage prevention is well established; however, the comparison of weight loss diets with different micronutrient and macronutrient profiles on genome stability in peripheral blood lymphocytes (PBLs) has not been studied. This study tested the hypothesis that genome stability in PBLs of ov...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gep006
更新日期:2009-05-01 00:00:00
abstract::Well-established in vitro methods for testing the genotoxic potency of chemicals--such as the Ames/Salmonella test, the mouse lymphoma assay, the micronucleus test and the chromosomal aberration test--show a high false-positive rate for predicting in vivo genotoxicity and carcinogenicity. Thus, there is a need for mor...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq040
更新日期:2010-11-01 00:00:00
abstract::The objective of the present study was to develop a cellular phenotype assay for nucleotide excision repair (NER), using benzo[a]pyrene diol epoxide (BPDE) as model mutagen. Since in vitro exposure to BPDE may lead to DNA strand breaks resulting from both direct interaction with DNA and incisions introduced by the rep...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gep039
更新日期:2010-01-01 00:00:00
abstract::Mutation induction by UV irradiation was studied in a retroviral vector integrated in one copy per cell at various chromosomal positions. As a mutational target, hamster hprt cDNA was present on the retroviral vector. To minimize the influence of repair we used repair-deficient hamster cells, V-H1 and UV5, as a recipi...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.5.399
更新日期:1993-09-01 00:00:00
abstract::Implementation of the seventh amendment to the EU Cosmetics Directive has driven much research into suitable in vitro alternative assays to support satisfactory risk assessments. One such assay is the reconstructed skin micronucleus (RSMN) assay. First reported in 2006, further development occurred and a standard prot...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gez037
更新日期:2019-12-03 00:00:00
abstract::The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent n...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.2.249
更新日期:1999-03-01 00:00:00
abstract::The clastogenic potential of sodium fluoride was determined both in vitro (using cultured human lymphocytes) and in vivo (using the rat bone-marrow micronucleus test). The incidence of chromosome aberrations in human lymphocyte cultures exposed to 20 or 40 micrograms/ml sodium fluoride (3 and 9% respectively) was sign...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.6.497
更新日期:1987-11-01 00:00:00
abstract::Down syndrome (DS) is the most common chromosomal abnormality. Many studies have assessed the association between maternal gene polymorphisms involved in folate metabolism and the risk of having a DS offspring, but data are conflicting. Our study aimed to arrive at a more accurate estimation. Therefore, we carried out...
journal_title:Mutagenesis
pub_type: 杂志文章,meta分析
doi:10.1093/mutage/get045
更新日期:2013-11-01 00:00:00
abstract::The effect of cerium oxide nanoparticle (CeNP) in plants has elicited substantial controversy. While some investigators have reported that CeNP possesses antioxidant properties, others observed CeNP to induce reactive oxygen species (ROS). In spite of considerable research carried out on the effects of CeNP in metazoa...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gey004
更新日期:2018-04-13 00:00:00
abstract::The development and use of chromosome-specific DNA probes to label entire human chromosomes has been an important advance in molecular cytogenetics. Fluorescence in situ hybridization using whole chromosome-specific DNA probes has been used to study both numerical and structural chromosomal aberrations in many human c...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.3.189
更新日期:1993-05-01 00:00:00