Abstract:
:The human FANCG/XRCC9 gene, which is defective in Fanconi anemia complementation group G (FA-G) cells, was first cloned by genetic complementation of the mitomycin C (MMC) sensitivity of CHO mutant UV40. The CHO NM3 mutant was subsequently assigned to the same complementation group. The parental AA8 CHO cells are hemizygous at the FancG locus, and we identified frameshift mutations that result in N-terminal truncations of the protein in both UV40 and NM3. Hypersensitivity to DNA cross-linking agents, such as MMC, typically characterizes FA cells. By introducing the native CHO FancG gene into mutant NM3, we demonstrate that hamster FancG fully corrects the 3-fold sensitivity to methyl methanesulfonate (MMS) as well as the 10-fold sensitivity to MMC, whereas resistance to ionizing radiation did not increase appreciably. In contrast, hamster cDNA transformants showed incomplete correction for both MMC and MMS sensitivity. The constitutively expressed FancG protein is present in the cytoplasmic, nuclear and chromatin fractions. FancG protein levels and subcellular localization do not change appreciably as a function of cell cycle position. Our results are consistent with roles of FancG in both the nuclear and cytoplasmic compartments to maintain genomic stability in response to various genotoxic agents.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Lamerdin JE,Yamada NA,George JW,Souza B,Christian AT,Jones NJ,Thompson LHdoi
10.1093/mutage/geh019subject
Has Abstractpub_date
2004-05-01 00:00:00pages
237-44issue
3eissn
0267-8357issn
1464-3804journal_volume
19pub_type
杂志文章相关文献
MUTAGENESIS文献大全abstract::In order to discriminate between different mechanisms put forward to explain the low observed yields of reciprocal translocation configurations at diakinesis-metaphase I spermatocytes following treatment of mouse stem cell spermatogonia with chemical clastogens, the physiological states of stem cells at the moment of ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/7.4.271
更新日期:1992-07-01 00:00:00
abstract::The old herbal drug aristolochic acid (AA), derived from Aristolochia spp., has been associated with the development of a novel nephropathy, designated aristolochic acid nephropathy (AAN), and urothelial cancer in AAN patients. There is clear evidence that the major components of the plant extract AA, aristolochic aci...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/17.4.265
更新日期:2002-07-01 00:00:00
abstract::Recent studies suggest increased cancer risk in patients with type 2 diabetes mellitus (T2DM) compared with healthy individuals. The present study aims to assess whether T2DM is associated with increased genome instability and whether a healthy diet with natural foods can improve genome stability in peripheral blood l...
journal_title:Mutagenesis
pub_type: 杂志文章,随机对照试验
doi:10.1093/mutage/ges073
更新日期:2013-03-01 00:00:00
abstract::The cytokinesis-block micronucleus cytome (CBMNCyt) assay is a widely used technique for measuring DNA damage in human populations. The formation of micronuclei (MN) in dividing cells can result from chromosome breakage due to unrepaired or mis-repaired DNA lesions or chromosome malsegregation due to mitotic malfuncti...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/geq076
更新日期:2011-01-01 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disorder of the elderly with a complex etiology due to the interaction between genetic and environmental factors. At least 15% of cases are inherited as an autosomal dominant mutation, but the majority are sporadic. We evaluated cytogenetic alterations, both spontaneous ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/16.4.323
更新日期:2001-07-01 00:00:00
abstract::We report on a new method for the study of radiation-induced chromatid breaks in stimulated human peripheral blood T lymphocytes, involving a combination of a 1-h colcemid block and a short (15 min) calyculin A treatment. We find that this procedure eliminates the problem of centromere splitting when calyculin A is us...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen009
更新日期:2008-07-01 00:00:00
abstract::Many chemical carcinogens require metabolic activation to form genotoxic compounds in human. Standard in vitro genotoxicity assays performed with activation systems, such as rat liver S9, are recognised to lead to a high number of false positives. The aim of this study was to evaluate the suitability of differentiated...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq039
更新日期:2010-11-01 00:00:00
abstract::A perceived disadvantage of transgenic rodent mutation assays is that spontaneous mutant frequencies are high compared to those of endogenous genes and may consequently reduce sensitivity to induced mutation. We have previously argued that unrepaired G:T mismatches from spontaneous deamination of 5-methylcytosine at C...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gen026
更新日期:2008-09-01 00:00:00
abstract::The effects of deficiency in the DNA mismatch repair (MMR) protein Pms2 were investigated using the endogenous mouse Hprt and Tk genes as reporters of intragenic mutation and loss of heterozygosity (LOH). Pms2(-/-)Tk(+/-), Pms2(+/+)Tk(+/-), Pms2(+/-)Tk(+/-) and Pms2(-/-)Tk(-/-) mice were bred from Pms2(+/-)Tk(+/-) mic...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geg007
更新日期:2003-07-01 00:00:00
abstract::The principal hydroxy-metabolites of benzene--hydroquinone, catechol and phenol--were assayed in tests for mitotic segregation induction in Aspergillus nidulans diploid strain 19. Hydroquinone was the most effective chemical, increasing the frequency of mitotic segregants up to 10-fold at 1-3 mM. Catechol was similarl...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/2.3.235
更新日期:1987-05-01 00:00:00
abstract::Basic extracts isolated from lamb meat treated at various temperatures were tested for mutagenicity in Salmonella typhimurium strain TA98 in the presence of S9 mix. Samples of ground lamb patties were cooked for 10 min per side at 100, 150, 200, 250 and 300 degrees C. Low cooking temperatures resulted in products with...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.3.228
更新日期:1989-05-01 00:00:00
abstract::The use of DNA adduct analysis has previously focused on the use of marine organisms for biomonitoring, whereas similar investigations in freshwater organisms are sparse. In that context, we have investigated the relevance of DNA adducts as biomarkers of genotoxicity in the freshwater mussels Dreissena polymorpha. The...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/ges036
更新日期:2012-11-01 00:00:00
abstract::The micronucleus test (MNT) is a well-established assay in genotoxicity testing and human biomonitoring. The cytokinesis-block micronucleus test (CBMNT) is the preferred method for measuring MN in cultured human lymphocytes from human subjects exposed to genotoxins. However, it is unclear to what extent mutagen exposu...
journal_title:Mutagenesis
pub_type: 杂志文章,评审
doi:10.1093/mutage/geq061
更新日期:2011-01-01 00:00:00
abstract::ISIS 2302, a phosphorothioate oligodeoxynucleotide with antisense activity against human ICAM-1 mRNA, was evaluated in a battery of tests to assess genotoxic potential. There was no evidence of genotoxicity in three in vitro studies performed: (i) a bacterial reverse mutation test; (ii) a chromosomal aberration test i...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/17.3.201
更新日期:2002-05-01 00:00:00
abstract::There are several reports demonstrating that aneugens may preferentially affect segregation of particular chromosomes in somatic cells. Much less is known on specific susceptibility of individual chromosomes to non-disjunction in mammalian meiosis in response to chemical exposures. To explore possible chromosome-speci...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/16.5.407
更新日期:2001-09-01 00:00:00
abstract::We have conducted a randomized trial which investigated the ability of dietary changes (in particular diets rich in cruciferous vegetables and flavonoids), to increase urinary antimutagenicity and inhibit DNA damage in smokers. Ninety heavy smokers were recruited and randomly assigned to three groups, and were given t...
journal_title:Mutagenesis
pub_type: 杂志文章,随机对照试验
doi:10.1093/mutage/gel021
更新日期:2006-05-01 00:00:00
abstract::The XPF and ERCC1 proteins form a tight complex and function as an endonuclease to incise on the 5'-side of pyrimidine dimers in DNA. Levels of both proteins are extremely low in group F xeroderma pigmentosum (XP-F) cells. We transfected XP-F cells with the plasmids expressing XPF or ERCC1 and examined levels of both ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.6.595
更新日期:1998-11-01 00:00:00
abstract::Monitoring oxidative stress in vivo is made easier by the ability to use samples obtained non-invasively, such as urine. The analysis of DNA oxidation, by measurement of oxidized 2'-deoxyribonucleosides in urine, particularly 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), has been reported extensively in the literatur...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq031
更新日期:2010-09-01 00:00:00
abstract::Well-established in vitro methods for testing the genotoxic potency of chemicals--such as the Ames/Salmonella test, the mouse lymphoma assay, the micronucleus test and the chromosomal aberration test--show a high false-positive rate for predicting in vivo genotoxicity and carcinogenicity. Thus, there is a need for mor...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geq040
更新日期:2010-11-01 00:00:00
abstract::Black tea theafulvins, a fraction of thearubigins isolated from black tea aqueous infusions, potentiated the mutagenic activity of the mycotoxin aflatoxin B(1) in the Ames test, in the presence of a hepatic S9 activation system derived from Aroclor 1254-treated rats. In contrast, when the S9 activation system was repl...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/18.2.145
更新日期:2003-03-01 00:00:00
abstract::We employed the three-way differential staining technique (TWD), which allows SCEs to be distinguished on a per generation basis by scoring third metaphases (M3), in order to study the spontaneous levels of SCEs in normal and high frequency cells (HFCs) that occurred in the first (S1), second (S2) and third (S3) S pha...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/12.5.329
更新日期:1997-09-01 00:00:00
abstract::Mutagens in the semivolatile phase of airborne particulate matter, diesel and gasoline engine emissions were investigated using chemical and biological assays. The previously unknown mutagens, 1-(3-)nitro-6-azabenzo[a]pyrenes (1-N-6-ABP and 3-N-6-ABP) and 1-(3-)nitro-6-azabenzo[a]pyrene-N-oxides (1-N-6-ABPO and 3-N-6-...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/9.1.47
更新日期:1994-01-01 00:00:00
abstract::Inter-individual differences in susceptibility to breast cancer are partially mediated through the levels of endogenous and exogenous steroid hormones. The CYP17 gene encodes P450c17alpha, an enzyme that is involved in the metabolism of steroid hormones. Increased endogenous steroid hormone levels have been associated...
journal_title:Mutagenesis
pub_type: 杂志文章,meta分析
doi:10.1093/mutage/17.2.119
更新日期:2002-03-01 00:00:00
abstract::The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent n...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/14.2.249
更新日期:1999-03-01 00:00:00
abstract::Alterations of DNA repair enzymes and consequential triggering of aberrant DNA damage response (DDR) pathways are thought to play a pivotal role in genomic instabilities associated with cancer development, and are further thought to be important predictive biomarkers for therapy using the synthetic lethality paradigm....
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gez051
更新日期:2020-02-13 00:00:00
abstract::In a sample of healthy subjects (23-59 years old), comprising 49 non-smokers and 23 smokers, we studied the effects of smoking on DNA repair synthesis (UDS) in peripheral lymphocytes. DNA repair after UV-irradiation was measured by [3H]TdR incorporation into DNA (semi-conservative synthesis was inhibited by hydroxyure...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/4.2.82
更新日期:1989-03-01 00:00:00
abstract::The aim of the present study was to develop and standardize a cytogenetic approach for evaluation of the mutagenic potential of chemicals that induce cell cycle arrest in the G2 phase. Even though cytogenetic end-points such as sister chromatid exchange (SCE) have been extensively used to indirectly assess the DNA-dam...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/geg034
更新日期:2003-11-01 00:00:00
abstract::As part of the hazard and risk assessment of chemicals in man, it is important to assess the ability of a chemical to induce mutations in vivo. Because of the commonalities in the molecular initiating event, mutagenicity in vitro can correlate well to the in vivo endpoint for certain compound classes; however, the dif...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/gey030
更新日期:2019-03-06 00:00:00
abstract::Transgenic B6C3F1 and C57BL/6 mice containing a lambda shuttle vector that carries a lacI target and an alpha lacZ reporter gene have been constructed for use in in vivo mutagenesis assays. After chemical treatment of mice carrying the lacI target gene, genomic DNA is isolated and the shuttle vector is recovered by ex...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/8.3.265
更新日期:1993-05-01 00:00:00
abstract::Carbaryl causes depolymerization of spindle microtubules and apparent uncoupling of karyokinesis and cytokinesis in mitotic V79 cells. The metabolite 1-naphthol has virtually identical effects at equimolar concentrations. The closely related 2-naphthol causes similar configurations, but at a much lower frequency than ...
journal_title:Mutagenesis
pub_type: 杂志文章
doi:10.1093/mutage/13.4.345
更新日期:1998-07-01 00:00:00