Abstract:
:The spectrum of mutations induced by cobalt(II) chloride (CoCl2) was examined using plasmid pUB3 DNA, which was propagated after transfection into Escherichia coli SY1032/pKY241 host cells. The vector plasmid carried an E.coli supF suppressor tRNA gene as a target for mutations. After CoCl2 treatment, 64 independent nalidixic acid-resistant, ampicillin-resistant and Lac+ (SupF-) clones were obtained and the altered sequences of the mutated supF genes were determined. Deletions and frameshifts were the predominant mutational event (61%) induced by CoCl2 and base substitutions were induced to a lesser degree (29%). Analysis of sequence alterations at all the sites of mutation revealed that: (i) 18 of 19 base substitutions and eight of 10 frameshifts occurred at G:C sites, suggesting that the formation of N7G-Co(II) adducts may be responsible for premutagenic lesions of these mutations; (ii) short sequence repeats were mostly found at the sites of deletions and frameshifts. Slippage-misalignment is also suggested to be a mechanism for the induction of mutations at these sites.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Ogawa HI,Ohyama Y,Ohsumi Y,Kakimoto K,Kato Y,Shirai Y,Nunoshiba T,Yamamoto Kdoi
10.1093/mutage/14.2.249subject
Has Abstractpub_date
1999-03-01 00:00:00pages
249-53issue
2eissn
0267-8357issn
1464-3804journal_volume
14pub_type
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